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Search Results: 1 - 10 of 8051 matches for " Felipe;Aguilera "
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Sylvatic triatominae: a new challenge in vector control transmission
Guhl, Felipe;Pinto, Nestor;Aguilera, Germán;
Memórias do Instituto Oswaldo Cruz , 2009, DOI: 10.1590/S0074-02762009000900012
Abstract: over the last 10 years, uruguay, chile and brazil have been certified as being free from disease transmission by triatoma infestans, the main domiciliated vector for chagas disease in the southern cone countries. this demonstrates that programmes addressing the vector for the disease's transmission are effective. these programmes have resulted in a dramatic decrease in the incidence of chagas disease in latin america. guatemala was certified a few months ago as being free from disease transmission by rhodnius prolixus, the main domiciliated vector for chagas disease in central american countries. however, the main concern for different countries' current control programmes is the continuity and sustainability of future vector control actions. the prevalence and incidence figures for individuals infected by trypanosoma cruzi in mexico and andean and central american countries highlights the need for broadened strategies in the struggle against the disease and its vectors. a number of triatomine insects are parasite vectors, each with a different life history. therefore, it is important that new vector control strategies be proposed, keeping in mind that some species are found in peridomiciliary areas and wild ecotopes. the only viable control strategy is to reduce human interactions with vector insects so that the re-infestation and re-colonisation of human habitats will not take place.
Actualización de la distribución geográfica y ecoepidemiología de la fauna de triatominos (Reduviidae: Triatominae) en Colombia
Guhl,Felipe; Aguilera,Germán; Pinto,Néstor; Vergara,Daniela;
Biomédica , 2007,
Abstract: information concerning triatomine records from provinces and municipalities was accumulated-including data indicating natural infections with trypanosomatides-that has been previously published or reported by colombian provincial health services and research institutes. altitude appeared to be the main factor responsible for the distribution of the insects. illustrations summarize the information provided by the above records. a triatomine fauna classification is presented that corresponds to the eco-epidemiological conditions of the country, considering altitude as the factor determining the geographical distribution of these vectors. rhodnius prolixus, triatoma dimidiata, triatoma maculata and triatoma venosa are considered the major transmission risk species in colombia, according to the frequency in which they are reported inside dwellings and peridomiciliary areas. entomological surveillance providess a necessary tool to reinforce the control strategies for chagas disease. this also allows the evaluation of transmission risk that the sylvatic triatomines represent in colombia.
Síndrome de Crouzon
Sonia,Ayala; Correa,Andres Felipe; Aguilera Castro,Fernando;
Revista Colombiana de Anestesiología , 2006,
Abstract: crouzon syndrome has a very low incidence in colombia, and very few cases are reported worldwide. little is known about the technical aspects of providing anesthesia for adult surgical patients with crouzon syndrome. a 41-year-old woman with crouzon syndrome was admitted to simon bolivar hospital for advanced craniofacial and maxillary reconstruction. she was treated by neurosurgical, maxillofacial surgical and anesthesia services. anesthetic and surgical management as well as treatment outcomes are presented. preanesthetic considerations, included airway maintenance, ophthalmologic evaluation and blood loss. perioperative management was a conventional one, with a combined technique. she was extubated in the intensive care unit with a smooth recovery. these techniques have been used with success in other patients with crouzon syndrome. a brief discussion of this case in the context of available literature is provided.
Estudio preliminar de la estructura genetica de Triatoma venosa (Hemiptera: Reduviidae) mediantemarcadores moleculares RAPD
ESPITIA,CLAUDIA; JARAMILLO,CARLOS; AGUILERA,GERMAN; PINTO,NESTOR; GUHL,FELIPE;
Revista Colombiana de Entomología , 2004,
Abstract: triatoma venosa is one of the main secondary vectors of chagas disease in colombia. to study its genetic structure, 30 adult individuals of t. venosa were collected in the municipalities of somondoco (villages of cabrera and barreras), guateque (villages of llano grande, tincachoque, and cantoras) and sutatenza (villages of sisquique, ovejeras, el gaque and piedra larga) department of boyaca, from two different habitats (peridomestic and domestic). genomic dna of each individual was amplified by rapd using 4 primers; the products were visualized after polyacrilamide gel electrophoresis followed by 6% silver staining. the dendrogram obtained after analysis with syntax 2000 and rapdplot software did not define a cluster for each insect population evaluated. when the binary matrix was analyzed with rapdfst software, the wright's fst was 0,047 and weir & cockerham's fst was 0,056. the effective migration rate in this analysis was 5,0 under wright's methodology and 4,2 under weir & cockerham's methodology. these preliminary results indicate a high mobility of the insects between the two habitats and a high genetic flow, which do not allow the establishment of genetic differentiation between the domestic and peridomestic populations analyzed.
Gas discharges from four remote volcanoes in northern Chile (Putana, Olca, Irruputuncu and Alitar): a geochemical survey
Franco Tassi,Felipe Aguilera,Orlando Vaselli,Thomas Darrah
Annals of Geophysics , 2011, DOI: 10.4401/ag-5173
Abstract: We analyzed gas samples collected from fumaroles and bubbling pools at Irruputuncu, Putana, Olca and Alitar volcanoes located in the central Andes volcanic zone (northern Chile). The Irruputuncu and Putana fumarolic discharges showed outlet temperatures ranging from 83 C to 240 C and from 82 C to 88 C, respectively. The chemical and isotopic (3He/4He, d13C-CO2, d18O-H2O and dD-H2O) compositions of these discharges were similar to medium-to-high temperature volcanic gases from other active volcanoes in this sector of the Andean volcanic chain (e.g. Lascar volcano). Inorganic and organic gas geothermometers for the H2O-CO2-CO-H2, CO2-CH4 and C2-C3 alkenes-alkanes systems indicated equilibrium temperatures that exceed 500 C at the gas sources. These relatively high temperatures are in agreement with the presence of relevantly high concentrations of magmatic gas emissions, including SO2. Olca and Alitar volcano fluid chemistries indicated lower amounts of magmatic-derived gas species, while both the helium and the water isotopic compositions suggested significant fractions of shallow, crustal/meteoric-originated fluids. These indicate contributions from a hydrothermal environment with temperatures <400 C. The geochemical and isotopic features derived from the present study show that the Irruputuncu, Putana, Olca and Alitar volcanoes should be considered as active and thus warrant periodic geochemical monitoring to determine the evolution of these systems and their potential hazards.
Updated geographical distribution and ecoepidemiology of the triatomine fauna (Reduviidae: Triatominae) in Colombia Actualización de la distribución geográfica y ecoepidemiología de la fauna de triatominos (Reduviidae: Triatominae) en Colombia
Felipe Guhl,Germán Aguilera,Néstor Pinto,Daniela Vergara
Biomédica , 2010,
Abstract: Information concerning triatomine records from provinces and municipalities was accumulated-including data indicating natural infections with trypanosomatides-that has been previously published or reported by Colombian provincial health services and research institutes. Altitude appeared to be the main factor responsible for the distribution of the insects. Illustrations summarize the information provided by the above records. A triatomine fauna classification is presented that corresponds to the eco-epidemiological conditions of the country, considering altitude as the factor determining the geographical distribution of these vectors. Rhodnius prolixus, Triatoma dimidiata, Triatoma maculata and Triatoma venosa are considered the major transmission risk species in Colombia, according to the frequency in which they are reported inside dwellings and peridomiciliary areas. Entomological surveillance providess a necessary tool to reinforce the control strategies for Chagas disease. This also allows the evaluation of transmission risk that the sylvatic triatomines represent in Colombia. La presente publicación recopila la información de registros de triatominos y datos sobre infección natural con tripanosomátidos a nivel departamental y municipal, publicada hasta la fecha así como la reportada por los servicios departamentales de salud e institutos de investigación. Se presentan figuras elaboradas de acuerdo a la información suministrada por los registros y una clasificación de la fauna triatomínica de acuerdo a las condiciones ecoepidemiológicas del país, teniendo en cuenta la altitud como factor determinante en la distribución de estos insectos. Teniendo en cuenta la frecuencia con que se reportan en el domicilio y peridomicilio, se consideran las siguientes especies como las de mayor riesgo de transmisión en Colombia: Rhodnius prolixus, Triatoma dimidiata, Triatoma maculata y Triatoma venosa. Se resalta la importancia de la vigilancia entomológica como herramienta indispensable para reforzar las estrategias de control de la transmisión de la enfermedad de Chagas, permitiendo también la evaluación del riesgo que representan las especies de triatominos silvestres en Colombia.
Síndrome de Crouzon
Ayala Sonia,Andres Felipe Correa,Fernando Aguilera Castro
Revista Colombiana de Anestesiología , 2006,
Abstract: El síndrome de Crouzon tiene una incidencia sumamente baja en nuestro medio y son pocos los casos que se reportan mundialmente. Poco se conoce acerca de técnicas anestésicas específicas y menos en pacientes en edad adulta. Se presenta el caso de una mujer de 41 a os con la enfermedad, para avance craneofacial y reconstrucción maxilar en varios tiempos hasta su estadio terapéutico final. El abordaje del caso se hizo por neurocirugía, cirugía maxilofacial y el grupo de anestesia del Hospital Simón Bolívar; en la valoración preanestésica se hizo énfasis en los predictores de vía aérea difícil, evaluación oftalmológica y en la pérdida sanguínea; se descartaron otras anomalías estructurales. El manejo transoperatorio inicial, que fue el mayor, se hizo convencionalmente con una técnica mixta; se trasladó a UCI para extubación programada, evolucionando satisfactoriamente. Se hicieron otros procedimientos para completar el avance basados en la misma técnica anestésica. La paciente evoluciona adecuadamente y es dada de alta. Se hace una discusión breve del caso, basados en la literatura disponible, concluyendo con algunas recomendaciones; se muestran los resultados del tratamiento. Crouzon syndrome has a very low incidence in Colombia, and very few cases are reported worldwide. Little is known about the technical aspects of providing anesthesia for adult surgical patients with Crouzon syndrome. A 41-year-old woman with Crouzon syndrome was admitted to Simon Bolivar Hospital for advanced craniofacial and maxillary reconstruction. She was treated by neurosurgical, maxillofacial surgical and anesthesia services. Anesthetic and surgical management as well as treatment outcomes are presented. Preanesthetic considerations, included airway maintenance, ophthalmologic evaluation and blood loss. Perioperative management was a conventional one, with a combined technique. She was extubated in the intensive care unit with a smooth recovery. These techniques have been used with success in other patients with Crouzon syndrome. A brief discussion of this case in the context of available literature is provided.
Multispectral Image Feature Points
Cristhian Aguilera,Fernando Barrera,Felipe Lumbreras,Angel D. Sappa,Ricardo Toledo
Sensors , 2012, DOI: 10.3390/s120912661
Abstract: This paper presents a novel feature point descriptor for the multispectral image case: Far-Infrared and Visible Spectrum images. It allows matching interest points on images of the same scene but acquired in different spectral bands. Initially, points of interest are detected on both images through a SIFT-like based scale space representation. Then, these points are characterized using an Edge Oriented Histogram (EOH) descriptor. Finally, points of interest from multispectral images are matched by finding nearest couples using the information from the descriptor. The provided experimental results and comparisons with similar methods show both the validity of the proposed approach as well as the improvements it offers with respect to the current state-of-the-art.
MOLECULAR ANALYSIS IN CHILEAN COMMERCIAL GASTROPODS BASED ON 16S rRNA, COI AND ITS1-5.8S rDNA-ITS2 SEQUENCES
Aguilera-Mu?oz,Felipe; Lafarga-Cruz,Fabiola; Gallardo-Escárate,Cristian;
Gayana (Concepción) , 2009, DOI: 10.4067/S0717-65382009000100003
Abstract: gastropod mollusks are part of the principal marine resources cultivated and commercialized in chile. there are native chilean species such as loco (concholepas concholepas), locate (thais chocolata), trumulco snail (chorus giganteus), keyhole limpets (fissurella spp.), tegula snail (tegula atra) as well as exotic species such as red abalone (haliotis rufescens) and japanese abalone (haliotis discus hannai). despite their importance as marine resources, molecular genetic studies establishing phylogenetic relationships and estimating population genetic parameters are scarce. the aim of this study is to establish a molecular approach among the main commercial gastropod species in chile. the mitochondrial genes 16s rrna and coi, and the nuclear ribosomal region itsl-5.8srdna-its2 were amplified by pcr and sequencing. alignment analysis was used to determine systematic relationships at the specific level for the species studied. the results revealed that 7 species are grouped in 4 genetically distinct families (haliotidae, trochidae, muricidae and fissurellidae). in comparison with coi sequencing, 16s rrna and itsl-5.8srdna-its2 sequencing were relatively more conserved with a divergence percentage for 16s rdna and itsl-5.8srdna-its2 of 1.2% and 1.8%, respectively, contrasting with the value of 10% obtained for coi in abalone.
AUTHENTICATION OF COMMERCIAL CHILEAN MOLLUSKS USING RIBOSOMAL INTERNAL TRANSCRIBED SPACER (ITS) AS SPECIE-SPECIFIC DNA MARKER
Aguilera-Mu?oz,Felipe; Valenzuela-Mu?oz,Valentina; Gallardo-Escárate,Cristian;
Gayana (Concepción) , 2008, DOI: 10.4067/S0717-65382008000200007
Abstract: contemporary world food safety standards have emphasized the implementation of efficient food traceability systems, including the correct labeling of products for the authentication of the commercialized species. in this context, chile has reached an important development in agricultural exportations, reaching near us$ 3.82 billion in 2007. since the identification based on morphologic and organoleptic characteristics is complex during the elaboration process of aquaculture products, molecular tools need to be developed in order to genetically trace aquaculture products. this study analyzed five species of chilean commercial mo husks using molecular dna markers. from the partial sequences of the ribosomal region itsl-5.8srdna-its2, the specific primers for the internal transcribed spacers 1 and 2 (its1, its2) were designed. these primers in 8 fresh species of mollusks, 5 commercial presentations and 3 processed species, obtaining specie-specific band patterns in all cases for both its. additionally, the primers are shown to be highly specific and of replicable amplification products, independently from the commercial presentation of the studied species. genetically trace aquaculture products. the presence of fraud for species substitution between canned abalone (haliotis) and loco (concholepas) species is discussed.
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