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Search Results: 1 - 10 of 68812 matches for " Felipe Rodrigues da;Vinecky "
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RBCS1 expression in coffee: Coffea orthologs, Coffea arabica homeologs, and expression variability between genotypes and under drought stress
Pierre Marraccini, Luciana P Freire, Gabriel SC Alves, Natalia G Vieira, Felipe Vinecky, Sonia Elbelt, Humberto JO Ramos, Christophe Montagnon, Luiz GE Vieira, Thierry Leroy, David Pot, Vania A Silva, Gustavo C Rodrigues, Alan C Andrade
BMC Plant Biology , 2011, DOI: 10.1186/1471-2229-11-85
Abstract: Expression of the RBCS1 gene was analysed in the allopolyploid context of C. arabica, which originates from a natural cross between the C. canephora and C. eugenioides species. Our study revealed the existence of two homeologous RBCS1 genes in C. arabica: one carried by the C. canephora sub-genome (called CaCc) and the other carried by the C. eugenioides sub-genome (called CaCe). Using specific primer pairs for each homeolog, expression studies revealed that CaCe was expressed in C. eugenioides and C. arabica but was undetectable in C. canephora. On the other hand, CaCc was expressed in C. canephora but almost completely silenced in non-introgressed ("pure") genotypes of C. arabica. However, enhanced CaCc expression was observed in most C. arabica cultivars with introgressed C. canephora genome. In addition, total RBCS1 expression was higher for C. arabica cultivars that had recently introgressed C. canephora genome than for "pure" cultivars. For both species, water stress led to an important decrease in the abundance of RBCS1 transcripts. This was observed for plants grown in either greenhouse or field conditions under severe or moderate drought. However, this reduction of RBCS1 gene expression was not accompanied by a decrease in the corresponding protein in the leaves of C. canephora subjected to water withdrawal. In that case, the amount of RBCS1 was even higher under drought than under unstressed (irrigated) conditions, which suggests great stability of RBCS1 under adverse water conditions. On the other hand, for C. arabica, high nocturnal expression of RBCS1 could also explain the accumulation of the RBCS1 protein under water stress. Altogether, the results presented here suggest that the content of RBCS was not responsible for the loss of photosynthetic capacity that is commonly observed in water-stressed coffee plants.We showed that the CaCe homeolog was expressed in C. eugenioides and non-introgressed ("pure") genotypes of C. arabica but that it was undetec
Brazilian coffee genome project: an EST-based genomic resource
Vieira, Luiz Gonzaga Esteves;Andrade, Alan Carvalho;Colombo, Carlos Augusto;Moraes, Ana Heloneida de Araújo;Metha, ?ngela;Oliveira, Angélica Carvalho de;Labate, Carlos Alberto;Marino, Celso Luis;Monteiro-Vitorello, Claúdia de Barros;Monte, Damares de Castro;Giglioti, éder;Kimura, Edna Teruko;Romano, Eduardo;Kuramae, Eiko Eurya;Lemos, Eliana Gertrudes Macedo;Almeida, Elionor Rita Pereira de;Jorge, érika C.;Albuquerque, érika V. S.;Silva, Felipe Rodrigues da;Vinecky, Felipe;Sawazaki, Haiko Enok;Dorry, Hamza Fahmi A.;Carrer, Helaine;Abreu, Ilka Nacif;Batista, Jo?o A. N.;Teixeira, Jo?o Batista;Kitajima, Jo?o Paulo;Xavier, Karem Guimar?es;Lima, Liziane Maria de;Camargo, Luis Eduardo Aranha de;Pereira, Luiz Filipe Protasio;Coutinho, Luiz Lehmann;Lemos, Manoel Victor Franco;Romano, Marcelo Ribeiro;Machado, Marcos Antonio;Costa, Marcos Mota do Carmo;Sá, Maria Fátima Grossi de;Goldman, Maria Helena S.;Ferro, Maria Inês T.;Tinoco, Maria Laine Penha;Oliveira, Mariana C.;Van Sluys, Marie-Anne;Shimizu, Milton Massao;Maluf, Mirian Perez;Eira, Mirian Therezinha Souza da;Guerreiro Filho, Oliveiro;Arruda, Paulo;Mazzafera, Paulo;Mariani, Pilar Drummond Sampaio Correa;Oliveira, Regina L.B.C. de;Harakava, Ricardo;Balbao, Silvia Filippi;Tsai, Siu Mui;Mauro, Sonia Marli Zingaretti di;Santos, Suzana Neiva;Siqueira, Walter José;Costa, Gustavo Gilson Lacerda;Formighieri, Eduardo Fernandes;Carazzolle, Marcelo Falsarella;Pereira, Gon?alo Amarante Guimar?es;
Brazilian Journal of Plant Physiology , 2006, DOI: 10.1590/S1677-04202006000100008
Abstract: coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. the genus coffea belongs to the rubiaceae family which includes other important plants. the genus contains about 100 species but commercial production is based only on two species, coffea arabica and coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. the brazilian coffee genome project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. we have single-pass sequenced a total of 214,964 randomly picked clones from 37 cdna libraries of c. arabica, c. canephora and c. racemosa, representing specific stages of cells and plant development that after trimming resulted in 130,792, 12,381 and 10,566 sequences for each species, respectively. the ests clustered into 17,982 clusters and 32,155 singletons. blast analysis of these sequences revealed that 22 % had no significant matches to sequences in the national center for biotechnology information database (of known or unknown function). the generated coffee est database resulted in the identification of close to 33,000 different unigenes. annotated sequencing results have been stored in an online database at http://www.lge.ibi.unicamp.br/cafe. resources developed in this project provide genetic and genomic tools that may hold the key to the sustainability, competitiveness and future viability of the coffee industry in local and international markets.
Rese a de "A memória da Copa de 70: esquecimentos e lembrancas do futebol na construcao da identidade nacional" de Marco Antonio Santoro Salvador y Antonio Jorge Goncalves Soares
Felipe Rodrigues da Costa,Wagner dos Santos
Movimento , 2011,
Abstract:
Mathematical Modeling for Isotherms of Mango Pulp Powder, Obtained by Atomization  [PDF]
érica Mil? de Freitas Felipe Rocha, Sueli Rodrigues, Marcos Rodrigues Amorim Afonso, José Maria Correia da Costa
Journal of Encapsulation and Adsorption Sciences (JEAS) , 2014, DOI: 10.4236/jeas.2014.41002
Abstract: Mango, as the other fruits, is a perishable food that can be easily deteriorated and presents high levels of post-harvest loss. Therefore, dehydration is an important alternative to use the production excess. In this context, this work aimed at evaluating the hygroscopic behavior of the integral mango pulp powder, obtained by atomization, by means of adsorption isotherm. After applying BET’s, GAB’s, Henderson’s and Oswin’s mathematical models, it was possible to identify that the equations for two models were perfectly adjusted to the experimental results of mango pulp powder. Henderson’s model was the best adjusted experimental curve and showed the lowest average error (E) and the highest determination coefficient (R2) at all temperatures studied.
Influência do grau de satura o na difus o de cloretos no concreto: vis o geral de sua importancia na
André Tavares da Cunha Guimar?es,Felipe Trez Rodrigues
Revista Teoria e Prática na Engenharia Civil , 2010,
Abstract:
Adipocyte Derived Mesenchymal Stromal Cell and Platelet Lysate: Ideal Cell and Supplement for the Treatment of Immune-Inflammatory Diseases?  [PDF]
Felipe Rodrigues, Annelise Pezzi, álvaro Laureano, Vanessa Valim, Bruna Zambonato, Alice Dahmer, Letícia Baggio, Filipe Sehn, Ianaê Wilke, Maria Aparecida L. da Silva, Bruna Amorin
CellBio (CellBio) , 2016, DOI: 10.4236/cellbio.2016.52002
Abstract: Background: Mesenchymal stromal cells (MSC) are being tested for the treatment of immune diseases. MSC are present in several adult tissues which milieu may influence MSC behavior particularly under inflammatory conditions. Additionally, culture conditions also can modify cell function or state of activation. Methods: To address the influence of the MSC source on its characteristics, we studied a xenofree, platelet lysate supplemented MSC from dental pulp, adipose tissue and bone marrow, co-cultured with isolated T cells and PBMC subset, and studied the effect of culture animal or human supplements immunomodulatory effect. Results: All three sources were efficient in inhibiting T cells. Among all MSC sources, as also described by others, adipose MSC was capable to significantly induce Treg phenotype and decrease T CD8+. Furthermore, comparing fetal bovine serum and platelet lysate, results demonstrate that platelet lysate alone is capable to induce immunomodulatory phenotype. Additional studies have to be made to elucidate the PL immunomodulatory effect.
3D Printed Scaffolds as a New Perspective for Bone Tissue Regeneration: Literature Review  [PDF]
Raquel Couto de Azevedo Gon?alves Mota, Emerson Oliveira da Silva, Felipe Fortes de Lima, Lívia Rodrigues de Menezes, Antonio Carlos Santos Thiele
Materials Sciences and Applications (MSA) , 2016, DOI: 10.4236/msa.2016.78039
Abstract: Due to the high incidence of bone fractures in the population, it became necessary to produce scaffolds that are able to assist in tissue regeneration. It is necessary to find an appropriate balance between the mechanical and biological properties, in order to mimic the natural tissue, these properties are directly related to the architecture and their degree of porosity, as well as the size of their pores and their interconnectivity. In this perspective, the 3D printing stands out, where the structure is obtained layer by layer, according to a predetermined computational model which provides a greater control of architecture and scaffold geometry and overcomes, in this way, the limitations of traditional techniques of scaffolds manufacturing. In this way, the objective of this seminar is to present the state of the art of the polymer scaffolds produced by 3D printing and applied to bone tissue regeneration, highlighting the advantages and limitations of this process.
Measurement of the optical density of packable composites: comparison between direct and indirect digital systems
Graziottin, Luiz Felipe Rodrigues;Costa, Nilza Pereira da;Silveira, Ivori Dutra da;Veeck, Elaine Bauer;
Pesquisa Odontológica Brasileira , 2002, DOI: 10.1590/S1517-74912002000400004
Abstract: the aim of this study was to measure the optical density of four packable composite resins with widths of 1, 2, 3 and 4 mm, by means of digora? (direct) and dentscan dentview? (indirect) digital imaging systems, in order to compare both methods. twenty acrylic plates, with the proposed thicknesses, were used, each one containing a sample of each resin. each acrylic plate was radiographed three times, under a standardized technique. for the digora? system, an optical plate was used under each resin sample, and, for the dentscan dentview? system, occlusal films were employed, totalizing 60 exposures for each system. optical plates and films were scanned and three consecutive optical readouts were carried out, totalizing 1,440 readouts. the results were submitted to statistical analysis and revealed that the average optical density of the four resins always increased as thickness increased. regarding the comparisons between the composite resins, in both analysis the resin with the greater optical density was surefil? followed by prodigy? condensable, alert? and solitaire?. the correlations between the results of digora? and dentscan dentview? were significant for the different thicknesses and materials. the observed tendency is that as the values obtained with the digora? system increase, so do the values obtained with dentscan dentview?. while thickness increased, the values of optical density in both digora? and dentscan dentview? tended to approach each other. the digora? system presented smaller amplitude between the results obtained in adjacent thicknesses.
Measurement of the optical density of packable composites: comparison between direct and indirect digital systems
Graziottin Luiz Felipe Rodrigues,Costa Nilza Pereira da,Silveira Ivori Dutra da,Veeck Elaine Bauer
Pesquisa Odontológica Brasileira , 2002,
Abstract: The aim of this study was to measure the optical density of four packable composite resins with widths of 1, 2, 3 and 4 mm, by means of Digora (direct) and DentScan DentView (indirect) digital imaging systems, in order to compare both methods. Twenty acrylic plates, with the proposed thicknesses, were used, each one containing a sample of each resin. Each acrylic plate was radiographed three times, under a standardized technique. For the Digora system, an optical plate was used under each resin sample, and, for the DentScan DentView system, occlusal films were employed, totalizing 60 exposures for each system. Optical plates and films were scanned and three consecutive optical readouts were carried out, totalizing 1,440 readouts. The results were submitted to statistical analysis and revealed that the average optical density of the four resins always increased as thickness increased. Regarding the comparisons between the composite resins, in both analysis the resin with the greater optical density was SurefilTM followed by ProdigyTM Condensable, AlertTM and Solitaire . The correlations between the results of Digora and DentScan DentView were significant for the different thicknesses and materials. The observed tendency is that as the values obtained with the Digora system increase, so do the values obtained with DentScan DentView . While thickness increased, the values of optical density in both Digora and DentScan DentView tended to approach each other. The Digora system presented smaller amplitude between the results obtained in adjacent thicknesses.
Spinning Gland Transcriptomics from Two Main Clades of Spiders (Order: Araneae) - Insights on Their Molecular, Anatomical and Behavioral Evolution
Francisco Prosdocimi,Daniela Bittencourt,Felipe Rodrigues da Silva,Matias Kirst,Paulo C. Motta,Elibio L. Rech
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0021634
Abstract: Characterized by distinctive evolutionary adaptations, spiders provide a comprehensive system for evolutionary and developmental studies of anatomical organs, including silk and venom production. Here we performed cDNA sequencing using massively parallel sequencers (454 GS-FLX Titanium) to generate ~80,000 reads from the spinning gland of Actinopus spp. (infraorder: Mygalomorphae) and Gasteracantha cancriformis (infraorder: Araneomorphae, Orbiculariae clade). Actinopus spp. retains primitive characteristics on web usage and presents a single undifferentiated spinning gland while the orbiculariae spiders have seven differentiated spinning glands and complex patterns of web usage. MIRA, Celera Assembler and CAP3 software were used to cluster NGS reads for each spider. CAP3 unigenes passed through a pipeline for automatic annotation, classification by biological function, and comparative transcriptomics. Genes related to spider silks were manually curated and analyzed. Although a single spidroin gene family was found in Actinopus spp., a vast repertoire of specialized spider silk proteins was encountered in orbiculariae. Astacin-like metalloproteases (meprin subfamily) were shown to be some of the most sampled unigenes and duplicated gene families in G. cancriformis since its evolutionary split from mygalomorphs. Our results confirm that the evolution of the molecular repertoire of silk proteins was accompanied by the (i) anatomical differentiation of spinning glands and (ii) behavioral complexification in the web usage. Finally, a phylogenetic tree was constructed to cluster most of the known spidroins in gene clades. This is the first large-scale, multi-organism transcriptome for spider spinning glands and a first step into a broad understanding of spider web systems biology and evolution.
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