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Search Results: 1 - 10 of 144315 matches for " Fantò F "
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Effectiveness and safety of citicoline in mild vascular cognitive impairment: the IDEALE study
Cotroneo AM, Castagna A, Putignano S, Lacava R, Fantò F, Monteleone F, Rocca F, Malara A, Gareri P
Clinical Interventions in Aging , 2013, DOI: http://dx.doi.org/10.2147/CIA.S38420
Abstract: tiveness and safety of citicoline in mild vascular cognitive impairment: the IDEALE study Original Research (3198) Total Article Views Authors: Cotroneo AM, Castagna A, Putignano S, Lacava R, Fantò F, Monteleone F, Rocca F, Malara A, Gareri P Video abstract presented by Pietro Gareri Views: 164 Published Date February 2013 Volume 2013:8 Pages 131 - 137 DOI: http://dx.doi.org/10.2147/CIA.S38420 Received: 22 September 2012 Accepted: 14 November 2012 Published: 05 February 2013 Antonino Maria Cotroneo,1 Alberto Castagna,2 Salvatore Putignano,3 Roberto Lacava,2 Fausto Fantò,4 Francesco Monteleone,5 Filomena Rocca,2 Alba Malara,6 Pietro Gareri2 1ASL 2 Turin, Piedmont, 2Elderly Health Care, Ambulatory Center for Dementia, ASP Catanzaro, Calabria, 3ASL Napoli 1, Campania, 4University Hospital Orbassano, Turin, Piedmont, 5Regina Margherita Hospital, Rome, 6Nursing Home S Domenico Lamezia Terme, ASP Catanzaro, Calabria, Italy Background: The studio di intervento nel decadimento vascolare lieve (IDEALE study) was an open multicenter Italian study, the aim of which was to assess the effectiveness and safety of oral citicoline in elderly people with mild vascular cognitive impairment. Methods: The study was performed in 349 patients. The active or citicoline group was composed of 265 patients and included 122 men and 143 women of mean age 79.9 ± 7.8 years selected from six Italian regions. Inclusion criteria were age ≥ 65 years, Mini-Mental State Examination (MMSE) score ≥ 21, subjective memory complaints but no evidence of deficits on MMSE, and evidence of vascular lesions on neuroradiology. Those with probable Alzheimer's disease were excluded. The control group consisted of 84 patients, including 36 men and 48 women of mean age 78.9 ± 7.01 (range 67–90) years. Patients included in the study underwent brain computed tomography or magnetic resonance imaging, and plasma dosage of vitamin B12, folate, and thyroid hormones. Functional dependence was investigated by scores on the Activities of Daily Living (ADL) and Instrumental Activities of Daily Living (IADL) scales, mood was assessed by the Geriatric Depression Scale (GDS), and behavioral disorders using the Neuropsychiatric Inventory scale. Comorbidity was assessed using the Cumulative Illness Rating Scale. An assessment was made at baseline (T0), after 3 months (T1), and after 9 months (T2, ie, 6 months after T1). The main outcomes were an improvement in MMSE, ADL, and IADL scores in the study group compared with the control group. Side effects were also investigated. The study group was administered oral citicoline 500 mg twice a day throughout the study. Results: MMSE scores remained unchanged over time (22.4 ± 4 at T0; 22.7 ± 4 at T1; 22.9 ± 4 at T2), whereas a significant difference was found between the study and control groups, both in T1 and in T2. No differences were found in ADL and IADL scores between the two groups. A slight but not statistically significant difference was found in GDS score be
Don Davide Albertario propagandista antiebraico : l'accusa di omicidio rituale / Don Davide Albertario and the antisemite propaganda : the blood libel.
Di Fant, Annalisa
Storicamente , 2011,
Abstract: The article analyses the leading role played by the priest/journalist Davide Albertario in the catholic antisemite propaganda of the late 19th century. He was director of L'Osservatore Cattolico , a Milan-based daily paper belonging to the catholic press network controlled by the Vatican, that published many articles against the Jews, notably involving the blood libel.
The Nexus of Prematurity, Birth Defects, and Intrauterine Growth Restriction: A Role for Plac1-Regulated Pathways
Michael E. Fant
Frontiers in Pediatrics , 2014, DOI: 10.3389/fped.2014.00008
Abstract: Epidemiological studies have demonstrated an increased prevalence of birth defects and intrauterine growth restriction (IUGR) among infants born prematurely suggesting they share common biological determinants. The identification of key regulatory pathways contributing to this nexus is essential to ongoing efforts to develop effective intervention strategies. Plac1 is a paternally imprinted and X-linked gene that conforms to this paradigm. Examination of a mutant mouse model has confirmed that Plac1 is essential for normal placental development and function. Moreover, it is expressed throughout the developing embryo indicating that it also has broad relevance to embryogenesis. Most notably, its absence in the developing embryo is associated with abnormal brain development and an increased risk of lethal, postnatal hydrocephalus identifying it as a novel, X-linked determinant of brain development. The essential and non-redundant roles of Plac1 in placental and neurological development represent a novel regulatory paradigm for embryonic growth and pregnancy maintenance. Regulatory pathways influenced, in part, by Plac1 are likely to contribute to the observed nexus of IUGR, prematurity, and birth defects.
A Case of Subacute Cutaneous Lupus Erythematosus in a Patient with Mixed Connective Tissue Disease: Successful Treatment with Plasmapheresis and Rituximab
M. Fantò,S. Salemi,F. Socciarelli,A. Bartolazzi,G. A. Natale,I. Casorelli,A. Pavan,S. Vaglio,R. Di Rosa,R. D'Amelio
Case Reports in Rheumatology , 2013, DOI: 10.1155/2013/857694
Abstract: A 30-year-old woman affected by Mixed Connective Tissue Disease with scleroderma spectrum developed a facial eruption, a clinical and histological characteristic of subacute cutaneous lupus erythematosus (SCLE). Speckled anti-nuclear antibodies, high-titer anti-ribonucleoprotein1, anti-Sm, anti-Cardiolipin (aCL) IgG/IgM, and anti-Ro/SSA antibodies were positive. SCLE was resistant to Azathioprine, Hydroxychloroquine, and Methotrexate while Mycophenolate Mofetil was suspended due to side effects. Subsequently, the patient was treated with three cycles of therapeutic plasma exchange (TPE) followed, one month after the last TPE, by the anti-CD20 antibody Rituximab (RTX) (375?mg/m2 weekly for 4 weeks). Eight and 16 months later the patient received other two TPE and RTX cycles, respectively. This therapeutic approach has allowed to obtain a complete skin healing persistent even after 8-month follow-up. Moreover, mitigation of Raynaud's phenomenon, resolution of alopecia, and a decline of aCL IgG/IgM and anti-Ro/SSA antibodies were observed. 1. Introduction Mixed Connective Tissue Disease (MCTD) is currently defined as an overlapping syndrome with clinical features of Systemic Sclerosis (SSc), Systemic Lupus Erythematosus (SLE), Rheumatoid arthritis (RA), and Polymyositis/Dermatomyositis (PM/DM); the presence of high-titer anti-ribonucleoprotein1 (U1RNP) or speckled anti-nuclear antibodies (ANA) at titer ≥1?:?2,000 is necessary for the diagnosis. The disease affects mainly women in the third decade of life (from 80 to 90%) but it has been also reported in children and in over-80-year-old people [1]. The most frequent clinical manifestations are Raynaud's phenomenon (RP), swollen hands, sclerodactyly, arthritis, myalgias, and oesophageal dysmotility, and also alopecia, malar rash, lymphadenopathy, or kidney damage can be present. Rarely, subacute cutaneous lupus erythematosus (SCLE), characterized by annular or papulosquamous lesions, photosensitivity, and presence of anti-Ro/SSA and anti-La/SSB antibodies, has been described in MCTD patients [2, 3]. MCTD therapy should be identified for each patient depending on the affected organ, but generally there is a good response to steroids, different types of vasodilators, and immunosuppressive agents such as Hydroxychloroquine (HCQ), Azathioprine (AZA), Methotrexate (MTX), or Cyclophosphamide (CYC) [1]. 2. Case Presentation A case of a thirty-year-old woman affected by MCTD with scleroderma spectrum and epilepsy since she was fifteen is here reported. At the beginning she presented fever up to 40°C, arthalgias
Variational Scheme to Compute Protein Reaction Pathways using Atomistic Force Fields with Explicit Solvent
S. a Beccara,L. Fant,P. Faccioli
Quantitative Biology , 2014,
Abstract: We introduce a variational approximation to the microscopic dynamics of rare conformational transitions of macromolecules. Within this framework it is possible to simulate on a small computer cluster reactions as complex as protein folding, using state of the art all-atom force fields in explicit solvent. We test this method against molecular dynamics (MD) simulations of the folding of an alpha- and a beta-protein performed with the same all-atom force field on the Anton supercomputer. We find that our approach yields results consistent with those of MD simulations, at a computational cost orders of magnitude smaller.
Nuclei of Non-Muscle Cells Bind Centrosome Proteins upon Fusion with Differentiating Myoblasts
Xavier Fant,Vlastimil Srsen,Aude Espigat-Georger,Andreas Merdes
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0008303
Abstract: In differentiating myoblasts, the microtubule network is reorganized from a centrosome-bound, radial array into parallel fibres, aligned along the long axis of the cell. Concomitantly, proteins of the centrosome relocalize from the pericentriolar material to the outer surface of the nucleus. The mechanisms that govern this relocalization are largely unknown.
The placental cholinergic system: localization to the cytotrophoblast and modulation of nitric oxide
Md Badiul Bhuiyan, Ferid Murad, Michael E Fant
Cell Communication and Signaling , 2006, DOI: 10.1186/1478-811x-4-4
Abstract: Using immunohistochemical techniques, ChAT was observed primarily within the cytotrophoblasts of preterm placentae as well as some mesenchymal elements. Similar intense immunostaining of the cytotrophoblast was observed for endothelium-derived nitric oxide synthase (eNOS) suggesting that ACh may interact with nitric oxide (NO)-dependent signaling pathways. The ability of carbamylcholine (CCh), an ACh analogue, to stimulate a rise in intracellular Ca++ and NO production in trophoblasts was therefore tested using the BeWob30 choriocarcinoma cell as a model system. First, CCh significantly increased intracellular calcium as assessed by fluorescence microscopy. We then examined the ability of CCh to stimulate NO production by measuring total nitrite/nitrate production in conditioned media using chemiluminescence-based analysis. CCh, alone, had no effect on NO production. However, CCh increased measurable NO approximately 100% in the presence of 10 nM estradiol. This stimulatory effect was inhibited by 1 (micro)M scopolamine suggesting mediation via muscarinic receptors. Estradiol, alone, had no effect on total NO or eNOS protein or mRNA.These data demonstrate that placental ChAT localizes to the cytotrophoblast and some mesenchymal cells in human placenta. It further suggests that ACh acts via muscarinic receptors on the trophoblast cell membrane to modulate NO in an estrogen-dependent manner.The presence of acetylcholine (ACh) in the human placenta, a non-innervated tissue, was first reported in 1933 by Chang and Gaddum [1]. Subsequent studies have documented the presence of all components of the cholinergic system in this tissue [see ref. [2] for a review]. The placenta-derived acetylcholine synthesizing enzyme, choline acetyltransferase (ChAT), was identified and reported by Comline in 1954 and purified to homogeneity by Hersh and Peete in 1977 [3,4]. Fant and Harbison later confirmed the presence of its degradative enzyme, acetylcholinesterase (AChE), and identified
Centrosome proteins form an insoluble perinuclear matrix during muscle cell differentiation
Vlastimil Srsen, Xavier Fant, Rebecca Heald, Catherine Rabouille, Andreas Merdes
BMC Cell Biology , 2009, DOI: 10.1186/1471-2121-10-28
Abstract: We performed studies on mouse myoblast cell lines that were induced to differentiate in culture, to characterize the early events of centrosome protein re-localization. We demonstrate that this re-localization occurs already at the single cell stage, prior to fusion into myotubes. Centrosome proteins that accumulate at the nuclear surface form an insoluble matrix that can be reversibly disassembled if isolated nuclei are exposed to mitotic cytoplasm from Xenopus egg extract. Our microscopy data suggest that this perinuclear matrix of centrosome proteins consists of a system of interconnected fibrils.Our data provide new insights into the reorganization of centrosome proteins during muscular differentiation, at the structural and biochemical level. Because we observe that centrosome protein re-localization occurs early during differentiation, we believe that it is of functional importance for the reorganization of the cytoskeleton in the differentiation process.The formation of muscle during embryonic development involves the differentiation of myoblasts into long, fibrous cells. In this differentiation process, myoblasts withdraw from the cell cycle and fuse into multinucleate, syncytial myotubes [1]. The microtubule cytoskeleton is reorganized from a radial network into a parallel array of filaments aligned along the long axis of the cells [2]. It is believed that this reorganization is a prerequisite for the elongation and fusion of myoblasts, and for the subsequent alignment and organization of sarcomeres [3-7]. Microtubule reorganization is paralleled by reorganization of centrosomal proteins: myoblasts possess a morphologically recognizable centrosome with characteristic marker proteins concentrated in the pericentriolar material, whereas myotubes show perinuclear localization of a multitude of centrosome proteins [8-10]. Consequently, polymerization of microtubules is initiated in part from the surface of the nucleus [8,10,11]. It has been reported that reorga
Otx2 ChIP-seq Reveals Unique and Redundant Functions in the Mature Mouse Retina
Alexander Samuel, Michael Housset, Bruno Fant, Thomas Lamonerie
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0089110
Abstract: During mouse retinal development and into adulthood, the transcription factor Otx2 is expressed in pigment epithelium, photoreceptors and bipolar cells. In the mature retina, Otx2 ablation causes photoreceptor degeneration through a non-cell-autonomous mechanism involving Otx2 function in the supporting RPE. Surprisingly, photoreceptor survival does not require Otx2 expression in the neural retina, where the related Crx homeobox gene, a major regulator of photoreceptor development, is also expressed. To get a deeper view of mouse Otx2 activities in the neural retina, we performed chromatin-immunoprecipitation followed by massively parallel sequencing (ChIP-seq) on Otx2. Using two independent ChIP-seq assays, we identified consistent sets of Otx2-bound cis-regulatory elements. Comparison with our previous RPE-specific Otx2 ChIP-seq data shows that Otx2 occupies different functional domains of the genome in RPE cells and in neural retina cells and regulates mostly different sets of genes. To assess the potential redundancy of Otx2 and Crx, we compared our data with Crx ChIP-seq data. While Crx genome occupancy markedly differs from Otx2 genome occupancy in the RPE, it largely overlaps that of Otx2 in the neural retina. Thus, in accordance with its essential role in the RPE and its non-essential role in the neural retina, Otx2 regulates different gene sets in the RPE and the neural retina, and shares an important part of its repertoire with Crx in the neural retina. Overall, this study provides a better understanding of gene-regulatory networks controlling photoreceptor homeostasis and disease.
PLAC1 Expression Decreases in Chorionic Villi in Response to Labor
Yahdira M. Rodriguez-Prado,Xiaoyuan Kong,Michael E. Fant
ISRN Obstetrics and Gynecology , 2013, DOI: 10.1155/2013/704252
Abstract: PLAC1 (Placenta-Specific 1) is a recently described, trophoblast-expressed gene essential for normal placental development. The protein localizes to the microvillus membrane surface of the syncytiotrophoblast in direct proximity to the maternal compartment. Although its role has not been defined, increased circulating levels of human PLAC1 mRNA in maternal blood are associated with preeclampsia. Furthermore, PLAC1-null mice exhibit decreased viability in the peripartum period suggesting a role in pregnancy maintenance late in gestation. We examined PLAC1 gene expression in the human placenta during normal pregnancy and pregnancies associated with maternal diabetes and preeclampsia using quantitative, real time PCR (q-RT-PCR). Although there was no apparent difference in PLAC1 gene expression among human pregnancies complicated by diabetes or preeclampsia, an unexpected effect of labor was noted at term. PLAC1 expression in placentae delivered vaginally following induced or spontaneous labor was significantly reduced compared to placentae not exposed to labor making it one of only a few placental genes influenced by labor. The significance of this finding is unknown. Viewed in the context of its importance in placental development, however, these findings are consistent with a role for PLAC1 in the maintenance of the maternal-fetal interface. 1. Introduction PLAC1 is a recently identified X-linked gene [1]. Compared to normal adult tissues, its expression is restricted primarily to cells of trophoblast lineage. The putative PLAC1 protein contains a signal peptide and a ZP3 motif (zona pellucida 3) suggesting it targets the secretory pathway and likely interacts with other membrane-associated proteins [2, 3]. Subsequent studies have confirmed its localization to the maternal surface of the syncytiotrophoblast, placing it in direct contact with the maternal compartment, suggesting it may be involved in protein interactions at the maternal-fetal interface [4]. Several studies have identified PLAC1 as a potential biomarker for gestational pathologies relevant to human health. Farina et al. (2005) first demonstrated that circulating PLAC1 mRNA in maternal blood was diminished in pregnancies associated with threatened abortion prior to 20 weeks gestation [5]. Subsequently, elevated levels of circulating PLAC1 mRNA were observed in pregnancies complicated by preeclampsia and were directly related to disease severity [6, 7]. Recently, we reported that women can become sensitized to the PLAC1 antigen during pregnancy and the presence of anti-PLAC1 antibodies may
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