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Search Results: 1 - 10 of 1809 matches for " Fabiano;ZANETTI "
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Calculating rabies virus neutralizing antibodies titres by flow cytometry
BORDIGNON, Juliano;COMIN, Fabiano;FERREIRA, Sílvia Córdoba P.;CAPORALE, Graciane M. M.;LIMA FILHO, José Hermênio Cavalcante;ZANETTI, Carlos Roberto;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2002, DOI: 10.1590/S0036-46652002000300007
Abstract: the determination of the rabies neutralizing antibody (vna) response after immunization against rabies is an acceptable index of the efficacy of a vaccine and a successful treatment. several tests have been developed in attempt to improve the assessment of vna, from mice inoculation to cell-culture fluorescence inhibition tests. all of them, however, present special difficulties in terms of reading or accuracy. the present study describes a neutralization test performed in cell-culture appraised by flow cytometry (fc). serial dilutions of the serum samples were mixed in vitro with rabies virus before the addition of bhk-21 cells. after 24h-incubation, cells were released by trypsin treatment, fixed and permeabilized with a p-formaldehyde solution and stained with a rabies virus nucleocapsid protein-specific antibody conjugate. the percentage of virus infection inhibition caused by specific antibodies present in the serum were evaluated in a beckton & dickinson facscalibur? flow cytometer. a correlation curve between the iu/ml content and the percentage of infective inhibition was built with a reference serum and the vna titers of serum samples were obtained by extrapolation. titers obtained by fc and standard test showed an effective pairing results (p < 0.01), with a correlation coefficient (r) = 0.7. these results permit to envisage the fc as a suitable technique to evaluate vna in sera from immunized animals and likely in human serum samples. nevertheless, new studies comparing fc to gold-standard techniques are required for determining the fc values of sensibility and specificity .
Antibody response in cattle after vaccination with inactivated and attenuated rabies vaccines
RODRIGUES da SILVA, Andréa de Cássia;CAPORALE, Graciane Maria Medeiros;GON?ALVES, Celso Alberto;TARGUETA, Mosar Couteiro;COMIN, Fabiano;ZANETTI, Carlos Roberto;KOTAIT, Ivanete;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2000, DOI: 10.1590/S0036-46652000000200006
Abstract: despite the absence of current official reports showing the number of cattle infected by rabies, it is estimated that nearly 30,000 bovines are lost each year in brazil. in order to minimize the important economic losses, control of the disease is achieved by eliminating bat colonies and by herd vaccination. in this study, we compare the antibody response in cattle elicited by vaccination with an attenuated era vaccine (aevac) and an inactivated-adjuvanted pv (ipvvac) vaccine. the antibody titers were appraised by cell-culture neutralization test and elisa, and the percentage of seropositivity was ascertained for a period of 180 days. ipvvac elicited complete seropositivity rates from day 30 to day 150, and even on day 180, 87% of the sera showed virus-neutralizing antibody titers (vna) higher than 0.5iu/ml. there were no significant differences between the vna titers and seropositivity rates obtained with ipvvac in the two methods tested. aevac, however, elicited significantly lower titers than those observed in the group receiving inactivated vaccine. in addition, the profiles of antirabies igg antibodies, evaluated by elisa, and vna, appraised by cell-culture neutralization test, were slightly different, when both vaccines were compared.
O intelectual modernista como artista: Rubén Darío
Zanetti, Susana;
Tempo Social , 2007, DOI: 10.1590/S0103-20702007000100002
Abstract: this article reviews the intellectual career of rubén darío, profiling the artist's position in modernism (1880-1910), the first concrete movement among hispano-american writers, which substantially renewed the literary output in spanish. incorporated into the modern market as a journalist and immigrant, the article follows darío's intellectual life from the archaic cultural context of his native nicaragua to some of the foremost hispano-american (buenos aires) and european (madrid, paris) centres, and considers his demands for aesthetic autonomy and his constant affirmation of the poet's authority to intervene in discussions relating to modern society and culture. here we see the emergence of his commitment to the creation of a latin america founded on new ties of solidarity and union with spain, in the face of the threatening advance of the united states.
Women navigating on Internet. Weaving in the age of the Web Donne in viaggio nella Rete. Tessiture al tempo di Internet
Federica Zanetti
Ricerche di Pedagogia e Didattica , 2011,
Abstract: The space offered by Internet as a place for developing new possibilities of exchange, of participation and of revindication may appear to have been “conquered” or “stolen” by women seeking to make a break with the tenets that continuously reconfirm gender stereotypes and to deconstruct those erotic-pornographic images in which the body of the woman is increasingly instrumentalised. It is an attempt to present an alternative to the distorted vision of sexuality involving forms of violence and exploitation which are increasingly found in the social space in Internet. If on one hand we cannot ignore the tendency to re-propose and reconfirm stereotypes linked to gender, that reinforce a dominant male culture, on the other, Internet can be recognized as a multi-perspective space made up of representations and auto-representations expressing a wide variety of points of view, in which culture and gender identity are constructed and transformed: a tool of female empowerment. Internet is also made up of female voices which live and tell. Women who navigate write, learn, create and maintain relationships. In fact, it is these women “navigators”, weavers of the Web, who are experimenting with new forms of nomadism, of research, of respect for their own culture and their own identity. The space offered by Internet as a place for developing new possibilities of exchange, of participation and of revindication may appear to have been “conquered” or “stolen” by women seeking to make a break with the tenets that continuously reconfirm gender stereotypes and to deconstruct those erotic-pornographic images in which the body of the woman is increasingly instrumentalised. It is an attempt to present an alternative to the distorted vision of sexuality involving forms of violence and exploitation which are increasingly found in the social space in Internet. If on one hand we cannot ignore the tendency to re-propose and reconfirm stereotypes linked to gender, that reinforce a dominant male culture, on the other, Internet can be recognized as a multi-perspective space made up of representations and auto-representations expressing a wide variety of points of view, in which culture and gender identity are constructed and transformed: a tool of female empowerment. Internet is also made up of female voices which live and tell. Women who navigate write, learn, create and maintain relationships. In fact, it is these women “navigators”, weavers of the Web, who are experimenting with new forms of nomadism, of research, of respect for their own culture and their own identity.
La Manufacture d’Armes de Saint-étienne : un patrimoine militaire saisi par l’économie créative
Thomas Zanetti
In Situ : Revue de Patrimoines , 2011, DOI: 10.4000/insitu.206
Abstract: Cet article prend pour objet d’analyse un projet de renouvellement urbain d’un espace hérité de l’activité industrielle et militaire : la Manufacture d’Armes de Saint-étienne. L’histoire et les caractéristiques patrimoniales de ce site, à la fois dans leurs dimensions matérielles et immatérielles, sont mobilisées dans un projet urbain hautement stratégique, et donc soumis à de multiples enjeux : la Cité du Design de Saint-étienne. Cet équipement s’insère quant à lui dans un cadre plus large de concurrence métropolitaine et de course à l’attractivité des territoires. En effet, dans une ville marquée par le traumatisme issu du déclin de l’industrie, l’existence d’un patrimoine militaire est con ue comme une opportunité de spécifier localement la mise en uvre des bonnes pratiques urbaines en circulation dans les villes européennes. Les responsables de la Cité ont donc choisi un haut lieu de l’histoire industrielle et militaire locale pour accueillir le futur symbole de la modernité stéphanoise, dans le but de tirer de cette inscription patrimoniale une plus-value historique nécessaire à la réussite du projet dans un contexte de marketing urbain. Toutefois, le processus de patrimonialisation de la Manufacture d’Armes a donné naissance à un profond conflit opposant des associations locales de protection du patrimoine aux responsables de l’urbanisme. Il importe alors de questionner l’entreprise de valorisation du patrimoine militaire en analysant quels sont les héritages mémoriels associés à l’action publique urbaine contemporaine, voire instrumentés par sa stratégie de développement territorial. This article is an analysis of a project of urban renewal concerning a space inherited from industrial and military activity: the arms factory of Saint-étienne. The history and the heritage characteristics of the site, in both its tangible and intangible dimensions, have been mobilized in a highly strategic urban project, the City of the Design, at the meeting point of many issues. This new design centre may be understood in a wider framework of metropolitan competition and the competition for the attractiveness of territories. Indeed, in a city marked by the traumatism stemming from the decline of the industry, the existence of a military heritage is seen as an opportunity to specify locally the implementation of the good urban practices in circulation in European cities. The persons in charge of the City thus chose a remarkable place of the local industrial and military history to welcome the future symbol of the local modernity, with the aim of benefittin
A Poética do Cotidiano em Curtas-metragens: os conflitos sociais entre o drama e a tragédia
ZANETTI, Daniela
Contemporanea : Revista de Comunica??o e Cultura , 2009,
Abstract: The article brings analyses of some award-winning short films that compose a sample of what is called the film periphery, presenting some features of this audiovisual production, shown in various festivals, and also available in the Internet. The content is part of a larger study, which seeks to map these audiovisual products, examine them and understand their ways of representation and of discursive production. The film analysis with focus on narrative strategies was used as the basis for the study of these short films, aiming to identify the ways of approach of the subjects like violence and drug trafficking. As a result, the films present some characteristics in common, specially with regard to the construction of the characters and of the represented spaces (slums, suburbs), and to the “moral” of the stories, which do not bring a comforting end. It is also important to note that the short films analysed do not represent the totality of the cinema of the periphery (which is heterogeneous), but a part of this particular production,specially the one related to the context of Rio de Janeiro city.
A cultura do compartilhamento e a reprodutibilidade dos conteúdos
Daniela Zanetti
Ciberlegenda , 2011,
Abstract: Diante de um acentuado discurso que coloca a convergência das mídias como fator ao mesmo tempo desestabilizador de estruturas tradicionais de veicula o de conteúdo na rede e também de inova o, o artigo desenvolve a ideia de “cultura do compartilhamento” no contexto da efetiva o das plataformas de arquivamento e de disponibiliza o dos mais diferentes tipos de conteúdos na rede, principalmente a partir do surgimento da Web 2.0. Para tanto, apresenta algumas características dessa prática recentemente institucionalizada e legitimada, relativas à sua constitui o no ambito da cibercultura.
Jovens feministas do Rio de Janeiro: trajetórias, pautas e rela??es intergeracionais
Zanetti, Julia Paiva;
Cadernos Pagu , 2011, DOI: 10.1590/S0104-83332011000100004
Abstract: throughout its history feminism was supported by many young generations, but only very recently young women began to claim knowledge, and opened discussions in specific areas within the youth movement, as black and lesbian feminists have done in previous decades. starting from the life stories of four young feminists in the rio de janeiro metropolitan area, this paper aims to contribute to the understanding of how young people have approached, inserted, and seen them selves within the movement.
El comercio azucarero cubano y la segunda guerra mundial
Zanetti Lecuona, Oscar;
América Latina en la historia económica , 2009,
Abstract: an analysis of the impact of second world war on cuban sugar commerce. since years before the war, cuba regulated sugar exports in connection with the american sugar quotas and the international sugar agreement signed in london, in 1937. the current article studies the adaptation of this sugar trade system to the war conditions, mainly when cuba sold the whole sugar harvest to the u.s., as well as the criteria of the principal interest groups in the island about this commercial policy and the future of sugar economy.
En camino al 98: Cánovas y el problema económico de Cuba (1878-1881)
Zanetti Lecuona, óscar
Revista de Indias , 1998,
Abstract: In 1878 the Cuban economy faced serious difficulties. The devastation caused by the first war of independence was aggravated by the negative trend affecting Cuba's staples in the international market. These economic problems ranked high among those that the Spanish administration was excepted to addres at the time, in introducing reforms in the colonial regime. The author analyzes the policy that the Spanish government adopted. En 1878, la economía cubana enfrentaba serias dificultades, A las devastaciones de la primera guerra independentista se unían tendencias muy desfavorables para la realización de los principales productos de la isla en el mercado internacional. Entre las reformas que se esperaba introduciría Espa a en su régimen colonial figuraban las que debían aportar soluciones a los problemas económicos de Cuba. El trabajo analiza la política seguida por el gobierno espa ol ante dicha situación.
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