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Search Results: 1 - 10 of 148717 matches for " Fábio Correia Sampaio "
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Prevalence and severity of dental fluorosis among students from Jo?o Pessoa, PB, Brazil
Carvalho, Thiago Saads;Kehrle, Helen Moura;Sampaio, Fábio Correia;
Brazilian Oral Research , 2007, DOI: 10.1590/S1806-83242007000300002
Abstract: the aim of this study was to determine the prevalence and severity of dental fluorosis among 12-15-year-old students from jo?o pessoa, pb, brazil before starting a program of artificial fluoridation of drinking water. the use of fluoridated dentifrice was also surveyed. a sample of 1,402 students was randomly selected. however, 31 students refused to participate and 257 were not permanent residents in jo?o pessoa, thus leaving a final sample of 1,114 students. clinical exams were carried out by two calibrated dentists (kappa = 0.78) under natural indirect light. upper and lower front teeth were cleaned with gauze and dried, and then examined using the tf index for fluorosis. a questionnaire on dentifrice ingestion and oral hygiene habits was applied to the students. the results revealed that fluorosis prevalence in this age group was higher than expected (29.2%). most fluorosis cases were tf = 1 (66.8%), and the most severe cases were tf = 4 (2.2%). the majority of the students reported that they had been using fluoridated dentifrices since childhood; 95% of the participants preferred brands with a 1,500 ppm f concentration, and 40% remembered that they usually ingested or still ingest dentifrice during brushing. it was concluded that dental fluorosis prevalence among students in jo?o pessoa is higher than expected for an area with non-fluoridated water. however, although most students use fluoridated dentifrices, and almost half ingest slurry while brushing, the majority of cases had little aesthetic relevance from the professionals' point of view, thus suggesting that fluorosis is not a public health problem in the locality.
Influence of Sub-Lethal and Lethal Concentrations of Chlorhexidine on Morphology and Glucosyltransferase Genes Expression in Streptococcus mutans UA159  [PDF]
Andréa Cristina Barbosa da Silva, Rafael Nóbrega Stipp, Renata de Oliveira Mattos-Graner, Fábio Correia Sampaio, Demetrius Ant?nio Machado de Araújo
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.413105
Abstract: Chlorhexidine (CHX) is regarded as one of the most successful antiplaque agents in controlling the formation of dental biofilm. Nevertheless, molecular mechanisms of their effects in Streptococcus mutans are largely unknown. In this work, the effects of sub-lethal and lethal concentrations of chlorhexidine (CHX) on planktonic or biofilm-organized Streptococcus mutans cells were investigated in dose- and time-dependent manner. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) for planktonic cells and biofilm conditions were determined by standard methods. Quantitative PCR (qPCR) was used to quantify the relative levels of glucosyltransferase B (gtfB), gtfC and gtfD transcription of S. mutans in the presence of CHX. The CHX activity in the initial biofilm structure and morphological alterations in planktonic cells were examined by Scanning Electron Microscopy (SEM). The results indicate that CHX increased expression of gtfC and gtfD in planktonic S. mutans cells and CHX reduced the expression of gtfB, gtfC, and gtfD in biofilms. High concentrations of CHX resulted in several wilted S. mutans planktonic cells with spilled intracellular material, while decreased cells’ chain length and matrix was found when the initial biofilm was exposed to increasing concentrations of CHX. CHX’s effects against bacteria depend on the type of growth organization and the concentration and time of exposure to the drug. At sub-lethal concentrations, CHX affects the expression of glucosyltransferases, which may have anticariogenic effect.
Urinary fluoride excretion in children exposed to fluoride toothpaste and to different water fluoride levels in a tropical area of Brazil
Forte, Franklin Delano Soares;Moimaz, Suzely Adas Saliba;Sampaio, Fábio Correia;
Brazilian Dental Journal , 2008, DOI: 10.1590/S0103-64402008000300007
Abstract: the aim of this study was to evaluate the urinary fluoride excretion of 2- to 7-year-old children exposed to different water fluoride concentrations in the city of catolé do rocha, pb, brazil. forty-two children were allocated to 3 groups according to the concentration of fluoride in the water: g1 (n=10; 0.5-1.0 ppm f), g2 (n=17; 1.1-1.5 ppm f) and g3 (n= 15; >1.51 ppm f). the study was carried out in two 1-week phases with 1-month interval between the moments of data collection: in the first phase, the children used a fluoride toothpaste (ft) (1,510 ppm f) for 1 week, whereas in the second phase a non-fluoride toothpaste (nft) was used. the urine was collected in a 24-h period in each week-phase according to marthaler's protocol. the urinary fluoride excretion data expressed as mean (sd) in μg/24 h were: g1-ft= 452.9 (290.2); g1-nft= 435.1 (187.0); g2-ft= 451.4 (224.0); g2-nft= 430.3 (352.5); g3-ft=592.3 (390.5); and g3-nft=623.6 (408.7). there was no statistically significant difference between the water fluoride groups, and regardless of the week phase (anova, p>0.05). the use of fluoride toothpaste (1,510 ppmf) did not promote an increase in urinary fluoride excretion. there was a trend, though not significant, as to the increase of urine fluoride concentration in relation to fluoride concentrations in the water. the excretion values suggest that some children are under risk to develop dental fluorosis and information about the appropriate use of fluoride is necessary in this area.
Natural fluoride levels from public water supplies in Piauí State, Brazil
Silva,Josiene Saibrosa da; Moreno,Wallesk Gomes; Forte,Franklin Delano Soares; Sampaio,Fábio Correia;
Ciência & Saúde Coletiva , 2009, DOI: 10.1590/S1413-81232009000600030
Abstract: the aim of this work was to determine the natural fluoride concentrations in public water supplies in piauí state, brazil, in order to identify cities in risk for high prevalence of dental fluorosis. for each city, two samples of drinking water were collected in the urban area: one from the main public water supply and another from a public or residential tap from the same source. fluoride analyses were carried out in duplicate using a specific ion electrode and tisab ii. from a total of 222 cities in piauí, 164 (73.8%) samples were analyzed. urban population in these towns corresponds to 92.5% of the whole state with an estimated population of 1,654,563 inhabitants from the total urban population (1,788,590 inhabitants). a total of 151 cities showed low fluoride levels (<0.30 mg/l) and 13 were just below optimum fluoride concentration in the drinking water (0.31-0.59 mg/l). high natural fluoride concentration above 0.81 mg/l was not observed in any of the surveyed cities. as a conclusion, most of the cities in piauí have low fluoride concentration in the drinking water. the risk for a high prevalence of dental fluorosis in these urban areas due to natural fluoride in the water supplies is very unlikely. thus, surveys about the dental fluorosis prevalence in piauí should be related with data about the consumption of fluoridated dentifrices and other fluoride sources.
Condi??o socioecon?mica e prevalência de más oclus?es em crian?as de 5 e 12 anos na USF Castelo Branco III: Jo?o Pessoa/Paraíba
Alves, Jo?o Anselmo de Oliveira;Forte, Franklin Delano Soares;Sampaio, Fábio Correia;
Revista Dental Press de Ortodontia e Ortopedia Facial , 2009, DOI: 10.1590/S1415-54192009000300008
Abstract: aim: to verify the prevalence of malocclusions and social-economic conditions of children aged 5 and 12 years old in the familiar health unit of castelo branco iii, jo?o pessoa/pb. methods: 49 children aged 5 and 12 years old were examined. the exams followed the methodology of the who (1997) and sb brazil (2001), and was used the criterion of the who (1997) and the dental aesthetic index (dai). results: it was found that most of the children had already gone to the dental office (mainly of the ones of the public network). the presence of malocclusions was observed in 33.3% of the 5 years old children and 40.7% of the 12 years old, there was no association with the gender in both ages (p > 0.05). most of the mothers presented education above 8 years (80.8% for the 5 years old children and 92.5% for the 12 years old), related income below two minimum salaries in both ages studied. it was observed the access to mouth health products (97.9% had toothbrush) and a good frequency of brushing was related (64.6% brushed 3 or more times a day). conclusion: there was no agreement between the criterion adopted for definition of malocclusion and self perception towards the appearance, treatment and mastication. facing this, it was necessary the implementation of public politics for prevention and orthodontic treatment in these children.
Hipossaliva o e aumento da glicose salivar em diabéticos
Adriana da Rosa Moreira,Maria Sueli Marques Soares,Isabela Albuquerque Passos,Fábio Correia Sampaio
Odonto , 2007,
Abstract: O objetivo desse trabalho foi avaliar o fluxo salivar e comparar as taxas de glicose salivar e sangüínea em diabéticos. A amostra foi constituída por 60 indivíduos, de ambos os sexos, com idade de 7 a 18 anos, sendo 30 diabéticos tipo 1, atendidos no servi o de endocrinologia pediátrica do PAM de Jaguaribe, Jo o Pessoa – PB/ Brasil, e 30 escolares, n o diabéticos, pareados em idade e sexo. Foram determinadas as taxas de fluxo salivar em repouso e estimulado, de glicose salivar e a glicemia capilar. Utilizou-se teste t de Student, considerando significantes valores de pd"0,05. Entre os pacientes diabéticos a média de idade foi de 13±2,8 anos, sendo 46,7% do sexo masculino e 53,3% do feminino. No controle, a média de idade foi de 11±2,8 anos, 53,3% eram do sexo masculino e 46,7% do feminino. A média da glicemia capilar entre os diabéticos foi de 216,6±119,5 mg/dL, enquanto que nos controles foi de 91±15,9mg/dL. A média da glicose salivar foi de 66 x 10-4 mg/dL nos diabéticos e 22 x 10-4 mg/dL no grupo controle (p=0,014). Nos diabéticos a média do FSR foi de 0,15±0,11 mL/min e no controle foi de 0,36±0,29 mL/min (p=0,001), enquanto a média do FSE foi de 2,32±1,03 mL/min nos diabéticos e 2,5±1,22 mL/min no controle (p=0,57). Concluiu-se que os diabéticos apresentaram níveis de glicose salivar mais elevada e redu o do fluxo salivar quando comparados aos n o diabéticos. Sugere-se que o diabetes altera a secre o e a composi o do fluxo salivar.
Minimum inhibitory concentration of adherence of Punica granatum Linn (pomegranate) gel against S. mutans, S. mitis and C. albicans
Vasconcelos, Laurylene César de Souza;Sampaio, Fábio Correia;Sampaio, Maria Carmélia Correia;Pereira, Maria do Socorro Vieira;Higino, Jane Sheila;Peixoto, Maria Helena Pereira;
Brazilian Dental Journal , 2006, DOI: 10.1590/S0103-64402006000300009
Abstract: the purpose of this study was to investigate the antimicrobial effect of a punica granatum linn (pomegranate) phytotherapeutic gel and miconazole (daktarin? oral gel) against three standard streptococci strains (mutans atcc 25175, sanguis atcc 10577 and mitis atcc 9811), s. mutans clinically isolated and candida albicans either alone or in association. the effect of minimum inhibitory concentrations of the gels on the adherence of these microorganisms to glass was assessed in the presence of 5% sucrose, using increasing and doubled concentrations of the diluted solution of the gels ranging from 1:1 to 1:1024. the minimum inhibitory concentrations of adherence of punica granatum l. gel against the test organisms were: 1:16 for s. mutans (atcc), s. mutans (ci) and s. sanguis; 1:128 for s. mitis and 1:64 for c. albicans. the minimum inhibitory concentrations of adherence of miconazole against the same organisms were: 1:512, 1:64, 1:4, 1:128 and 1:16, respectively. in experiments with three and four associated microorganisms, the punica granatum l. gel had greater efficiency in inhibiting microbial adherence than the miconazole. the results of this study suggest that this phytotherapeutic agent might be used in the control of adherence of different microorganisms in the oral cavity.
Radiographic diagnosis of incipient proximal caries: an ex-vivo study
Silva Neto, José Moreira da;Santos, Rosenês Lima dos;Sampaio, Maria Carmeli Correia;Sampaio, Fábio Correia;Passos, Isabela Albuquerque;
Brazilian Dental Journal , 2008, DOI: 10.1590/S0103-64402008000200002
Abstract: the aim of this ex vivo study was to compare visual clinical and radiographic examinations to the histological analysis for proximal caries diagnosis in extracted permanent molars and premolars. the relationship between clinical aspects and carious lesions was also evaluated. eighty-eight proximal surfaces (44 freshly extracted teeth) were longitudinally sectioned with a 370-μm diamond disk, thinned with wet silicon carbide paper and observed with a stereomicroscope at x40 magnification. sensitivity and specificity were 65.6% and 83.3% for clinical examination and 29.7% and 95.8% for radiographic examination, respectively. kappa values ranged from 0.64 to 0.91. the white spots corresponded to lesions restricted to enamel, while the dark spots corresponded to lesions that reached the dentinoenamel junction. in most cases, cavitation corresponded to dentin lesions. it may be concluded that interproximal radiographic examination is not a reliable method for detection of incipient proximal carious lesions.
Streptococcus mutans in denture stomatitis patients under antifungal therapy
Vasconcelos, Laurylene César de S.;Sampaio, Fábio Correia;Sampaio, Maria Carméli Correia;Pereira, Maria do Socorro Vieira;Peixoto, Maria Helena Pereira;
Revista Odonto Ciência , 2010, DOI: 10.1590/S1980-65232010000200003
Abstract: purpose: to assess the number of streptococcus mutans in saliva of patients with denture stomatitis before and after antifungal therapy. methods: after examining 93 patients, 47 were selected for fungal test. then, from this sample, thirty patients were selected: 15 with positive and 15 with negative diagnosis for candidiasis that were evaluated for s. mutans counting, salivary flow and buffer capacity evaluation. oral hygiene and prosthesis hygiene, period using prosthesis, lesion type and salivary data were related with clinical laboratorial characteristics of the patients with candida. results: the most frequent lesions were type i (43.5%) and ii (53.5%). the amount of s. mutans was six times higher in patients with candidiasis and it was associated with low salivary flow and poor oral hygiene. after therapy, a reduction of s. mutans was verified particularly in patients with normal salivary flow. the values ranged from 0.01 to 3.88 x 104 cfu/ml. conclusion: the data suggest that streptococcus spp collaborates with candida spp in the etiology and pathogenesis of denture stomatitis. the use of oral antimicrobial agents may provide a beneficial effect for denture stomatitis patients that are under antifungal therapy and that have poor oral hygiene and unfavorable salivary parameters.
Fluoride intake from drinking water and dentifrice by children living in a tropical area of Brazil
Omena, Leila Maria F.;Silva, Milton F. de A.;Pinheiro, Cleone Calheiros;Cavalcante, Jairo C.;Sampaio, Fábio Correia;
Journal of Applied Oral Science , 2006, DOI: 10.1590/S1678-77572006000500015
Abstract: objective: to assess fluoride (f-) intake from water and toothpaste by children aged 18 to 36 months and to monitor the f- concentrations in the drinking water system in a tropical city of brazil. methods: children (n=58) aged 18-36 months, all lifetime residents of penedo, state of alagoas, brazil, participated in this study. water f- analyses were carried out in 7 different occasions at least a week apart. for 2 days all the water drunk by each child was accounted for. fluoride intake from water for each child was estimated using the mean f- concentration of water in 7 different occasions. fluoride intake from toothpaste was estimated by subtracting the recovered post-brushing f- from the original amount placed in the toothbrush. the f- intake from water and toothpaste was estimated by dividing the total amount of f- ingested by the weight of each child. results: the mean f- concentration in the drinking water was 0.94 ppm (mean range 0.78-1.1 ppm), which is above the 0.7 ppm recommended for this area of brazil. mean total f- intake from water and toothpaste was 0.128 mg f-/kg body weight/day. the daily means of f- intake from water and toothpaste were 0.021 and 0.107 mg f-/kg body weight, respectively. ninety six percent of children showed f- intake above 0.07 mg f-/kg body weight/day. conclusions: children in penedo are at risk for developing dental fluorosis due to high f- intake from fluoridated toothpastes. water fluoridation showed low contribution to the total f- intake. however, high water f- concentrations in the water indicate the need of surveillance of the artificial water fluoridation system.
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