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Search Results: 1 - 10 of 176751 matches for " Evandro Leite de; "
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Linfoma n?o-Hodgkin em tireóide: relato de caso
Azambuja, Evandro de;Azevedo, Sérgio Jobim de;Moreira, Roger;Castro, Rodrigo C. Leite de;
Arquivos Brasileiros de Endocrinologia & Metabologia , 2004, DOI: 10.1590/S0004-27302004000300014
Abstract: the authors describe the case of a 33 year-old white female, without any clinical or laboratorial evidence of thyroiditis or clinical hypothyroidism, who presented with a rapidly enlarging anterior neck mass. diagnosis of a follicular non-hodgkin's lymphoma was made through histopathological and immunohistochemical analysis.
Effect of cinnamomum zeylanicum blume essential oil on the rowth and morphogenesis of some potentially pathogenic Aspergillus species
Carmo, Egberto Santos;Lima, Edeltrudes de Oliveira;Souza, Evandro Leite de;Sousa, Frederico Barbosa de;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000100021
Abstract: cinnamomum zeylanicum blume is known for a wide range of medicinal properties. this study aimed to assess the interference of c. zeylanicum essential oil on the growth and morphogenesis of some potentially pathogenic aspergillus species. the essential oil presented strong antifungal effect causing the growth inhibition of the assayed strains and development of large growth inhibition zones. mic50 and mic90 values were 40 and 80 μl/ml, respectively. 80, 40 and 20 μl/ml of the oil strongly inhibited the radial mycelial growth of a. niger, a. flavus and a. fumigatus along 14 days. 80 and 40 μl/ml of the oil caused a 100% inhibition of the fungal spore germination. main morphological changes observed under light microscopy provided by the essential oil in the fungal strains were decreased conidiation, leakage of cytoplasm, loss of pigmentation and disrupted cell structure indicating fungal wall degeneration. it is concluded that c. zeylanicum essential oil could be known as potential antifungal compound, particularly, to protect against the growth of aspergillus species.
Bacteriocins: molecules of fundamental impact on the microbial ecology and potential food biopreservatives
Souza, Evandro Leite de;Silva, Clemilson Antonio da;Sousa, Cristina Paiva de;
Brazilian Archives of Biology and Technology , 2005, DOI: 10.1590/S1516-89132005000500008
Abstract: bacteriocins are proteic molecules synthesized for various lineages of gram-positive and gram-negative bacteria when exposed to stressful conditions. bacteriocins have been characterized as molecules of high antimicrobial property even at low concentrations, provoking the microbial survival inhibition by antibiosis. these substances have their synthesis mediated for genetic mechanisms and develop their lethal action on the microbial cell by multiples mechanisms that can act of isolated or concomitant way culminating with microbial cell killing. this molecules class presents characteristic of stability to heat, low ph, refrigeration and freezing, and resistance to weak organics solvents, salts and enzymes. on the other hand, they are very sensitive to proteolytic enzymes action. bacteriocins could appear as potential agents to be applied in food conservation systems in order to provide microbiologically stable foods.
The potential of Origanum vulgare L. (Lamiaceae) essential oil in inhibiting the growth of some food-related Aspergillus species
Carmo, Egberto Santos;Lima, Edeltrudes de Oliveira;Souza, Evandro Leite de;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000200030
Abstract: origanum vulgare l. (lamiaceae) has been currently known for their interesting antimicrobial activity being regarded as alternative antimicrobial for use is food conservation systems. this study aimed to evaluate the effectiveness of o. vulgare essential oil in inhibiting the growth of some food-related aspergillus species (a. flavus, a. parasiticus, a. terreus, a. ochraceus, a. fumigatus and a. niger). the essential oil revealed a strong anti-aspergillus property providing an inhibition of all assayed mould strains. mic values were between 80 and 20 μl/ml being found a mic50 of 40 μl/ml. the essential oil at concentration of 80 and 40 μl/ml provided a fungicidal effect on a. flavus, a. fumigatus and a. niger noted by a total inhibition of the radial mycelial growth along 14 days of interaction. in addition, the essential oil was able to inhibit the mould spores germination when assayed at concentrations of 80 and 40 μl/ml. our results showed the interesting anti-aspergillus activity of o. vulgare essential oil supporting their possible use as anti-mould compound in food conservation.
Sensitivity of spoiling and pathogen food-related bacteria to Origanum vulgare L. (Lamiaceae) essential oil
Souza, Evandro Leite de;Stamford, Tania Lúcia Montenegro;Lima, Edeltrudes de Oliveira;
Brazilian Journal of Microbiology , 2006, DOI: 10.1590/S1517-83822006000400023
Abstract: origanum vulgare l. (oregano), lamiaceae, has been known as plant specie with prominent biological properties for a long time. this study aimed to evaluate the antibacterial activity of origanum vulgare essential oil on various gram-positive and gram-negative spoiling and/or pathogen food-related bacteria, as well as to observe its antimicrobial effectiveness in a food conservation micromodel. the results showed a strong antibacterial activity of the assayed essential oil noted by large growth inhibition zones (30-37 mm). mic values were between 20-40 μl/ml for the most bacteria strains. essential oil was able to cause significant (p<0.05) inhibitory effect on the bacteria viability providing a bacteriostatic effect after 24hours of exposure. in addition, the mic provided a significant (p<0.05) decrease of the autochthonous bacterial flora in ground meat samples stored under refrigeration. these results support the possibility of using origanum vulgare essential oil as alternative antimicrobial compound in food conservation systems.
Inhibitory effect of the essential oil from Cinnamomum zeylanicum Blume leaves on some food-related bacteria
Trajano, Vinicius Nogueira;Lima, Edeltrudes de Oliveira;Travassos, Ant?nio Eustáquio;Souza, Evandro Leite de;
Ciência e Tecnologia de Alimentos , 2010, DOI: 10.1590/S0101-20612010000300032
Abstract: cinnamomum zeylanicum blume, lauraceae, has long been known for having many biological properties. this study aimed to identify the constituents of the essential oil from c. zeylanicum leaves using gc-ms and to assess its inhibitory effect on salmonella enterica, escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa based on mic and mbc determination and kill-time study. eugenol (73.27%) was the most prevalent compound in the essential oil followed by trans-β-cariophyllene (5.38%), linalool (3.31%), and alcohol cinamic acetate (2.53%). the results showed an interesting antibacterial activity of the oil with mic ranging from 1.25 to 10 μl.ml-1. mbc values were in the range of 20 - 80 μl.ml-1. a concentration of 10 and 40 μl.ml-1 of the essential oil caused a fast and steady decrease in viable cell count (2 to 5 log cycles) of all assayed strains along 24 hours. a concentration of 40 μl.ml-1 of the oil provided a total elimination of the initial inocula of s. aureus after 2 hours. these results show the possibility of regarding the essential oil from c. zeylanicum leaves as alternative sources of antimicrobial compounds to be applied in food conservation systems.
Inhibitory action of some essential oils and phytochemicals on the growth of various moulds isolated from foods
Souza, Evandro Leite de;Lima, Edeltrudes de Oliveira;Freire, Kristerson Reinaldo de Luna;Sousa, Cristina Paiva de;
Brazilian Archives of Biology and Technology , 2005, DOI: 10.1590/S1516-89132005000200011
Abstract: the aim of this study was to determine the sensitivity profile of mould strains isolated from foods to some essential oils and phytochemicals. the assayed mould strains were: fusarium spp., rhizopus spp., aspergillus flavus, a. niger and penicillium spp. according to results, lippia alba n.e. brown, peumus boldus molina, lippia microphylla phil., citrus limon risso and cymbopogon citratus stapf. essential oil and the phytochemicals citral, eugenol and mircene showed prominent antimould activity. among the products that evidenced antimould activity, citral and eugenol showed the lowest minimum inhibitory concentrations, which was 1% and 4%, respectively, for the most of the tested mould strains.
Anti-staphylococcal effectiveness of nisaplin in refrigerated pizza doughs
Freitas, Wilma Carla de;Souza, Evandro Leite de;Sousa, Cristina Paiva de;Travassos, Ant?nio Eustáquio Resende;
Brazilian Archives of Biology and Technology , 2008, DOI: 10.1590/S1516-89132008000300021
Abstract: this study evaluated the effectiveness of nisaplin, commercial product having nisin as active component, in decreasing the staphylococcal population in refrigerated pizza doughs. the refrigerated pizza dough pieces randomly chosen were dipped in the solutions with nisaplin concentrations of 1.0 x 10-3 g and 1.0 x 10-2 g nisaplin/ml named for the treatment a and b and kept under refrigeration (7 °c ± 1 °c). on times 0, 15 and 30 days post treatment the staphylococcus spp. count was carried out. the results showed that both nisaplin treatments were able to reduce the staphylococcus spp. count (cfu/g) in the refrigerated pizza doughs. however, only treatment b showed statistically significant reducer effect (p < 0.05) on the count providing a decrease of 1.0 and 0.98 log cycles, respectively, after 15 and 30 days post treatment. these data suggest that nisin could appear as promising alternative to control the survival of the pathogen microorganisms in the foods, particularly, staphylococcus in the refrigerated pizza doughs.
Inhibitory effect of beta-pinene, alpha-pinene and eugenol on the growth of potential infectious endocarditis causing Gram-positive bacteria
Leite, Aristides Medeiros;Lima, Edeltrudes de Oliveira;Souza, Evandro Leite de;Diniz, Margareth de Fátima Formiga Melo;Trajano, Vinícius Nogueira;Medeiros, Isac Almeida de;
Revista Brasileira de Ciências Farmacêuticas , 2007, DOI: 10.1590/S1516-93322007000100015
Abstract: this study was led with the purpose of evaluating the effectiveness of eugenol, b-pinene and a-pinene in inhibiting the growth of potential infectious endocarditis causing gram-positive bacteria. the phytochemicals minimum inhibitory concentration-mic was determined by solid medium diffusion procedure, while the interference of the mic values on the bacterial cell viability was performed by viable cells count. staphylococcus aureus, s. epidermidis, streptococcus pneumoniae and s. pyogenes strains were used as test microorganisms. the assayed phytochemicals showed effectiveness in inhibiting all assayed bacteria strains presenting mic values between 2.5 and 40 μl/ml. eugenol showed the lowest mic values which were between 2.5 and 5 μl/ml for the most bacteria strains. mic values found to the phytochemicals were able to inhibit the cell viability of s. aureus providing a total elimination of the bacteria inoculum in a maximum time of 24 hours of exposure. these data showed the interesting antibacterial property of the assayed phytochemicals and support their possible and rational use in the antimicrobial therapy.
Preliminary study of the molluscicidal and larvicidal properties of some essential oils and phytochemicals from medicinal plants
Leite, Aristides M.;Lima, Edeltrudes de O.;Souza, Evandro L. de;Diniz, Margareth de F. F. M.;Leite, S?nia Pereira;Xavier, Aline L.;Medeiros, Isac A. de;
Revista Brasileira de Farmacognosia , 2009, DOI: 10.1590/S0102-695X2009000600008
Abstract: this study aimed to evaluate the molluscicidal and larvicidal activity of some essential oils and phytochemicals from medicinal plants. molluscicide and larvicidal activity were determined by, respectively, the lethality bioassays using artemia salina leach. artemiidae and aedes aegypti l. culicidae larvae. essential oils from eugenia uniflora l. myrtaceae, laurus nobilis l. lauraceae, origanum vulgare l. lamiaceae and the phytochemicals α-pinene and eugenol presented citotoxicity toward artemia salina with cl50 values between 9.59 and 253.43 μl/ml. essential oils from e. uniflora, m. piperita, o. vulgare and r. officinalis showed embryotoxicity on aedes aegypti larvae with a viability inhibition between 40 and 100%. these results show the bioactivity of the assayed essential oils and phytochemicals and, partially, justify their insertion in further evaluation in order to establish a safe exploitation of their biological potentiality.
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