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Search Results: 1 - 10 of 3966 matches for " Eun Chul Jang "
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Enhancement in electron transport and light emission efficiency of a Si nanocrystal light-emitting diode by a SiCN/SiC superlattice structure
Chul Huh, Bong K Kim, Byoung-Jun Park, Eun-Hye Jang and Sang-Hyeob Kim
Nanoscale Research Letters , 2013, DOI: 10.1186/1556-276X-8-14
Abstract: We report an enhancement in light emission efficiency of Si nanocrystal (NC) light-emitting diodes (LEDs) by employing 5.5 periods of SiCN/SiC superlattices (SLs). SiCN and SiC layers in SiCN/SiC SLs were designed by considering the optical bandgap to induce the uniform electron sheet parallel to the SL planes. The electrical property of Si NC LED with SiCN/SiC SLs was improved. In addition, light output power and wall-plug efficiency of the Si NC LED with SiCN/SiC SLs were also enhanced by 50% and 40%, respectively. This was attributed to both the formation of two-dimensional electron gas, i.e., uniform electron sheet parallel to the SiCN/SiC SL planes due to the conduction band offset between the SiCN layer and SiC layer, and an enhanced electron transport into the Si NCs due to a lower tunneling barrier height. We show here that the use of the SiCN/SiC SL structure can be very useful in realizing a highly efficient Si NC LED.
Efficient Production of Retroviruses Using PLGA/bPEI-DNA Nanoparticles and Application for Reprogramming Somatic Cells
Eun Jin Seo, Il Ho Jang, Eun Kyoung Do, Hyo Cheon Cheon, Soon Chul Heo, Yang Woo Kwon, Geun Ok Jeong, Ba Reun Kim, Jae Ho Kim
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0076875
Abstract: Reprogramming of somatic cells to pluripotent cells requires the introduction of factors driving fate switches. Viral delivery has been the most efficient method for generation of induced pluripotent stem cells. Transfection, which precedes virus production, is a commonly-used process for delivery of nucleic acids into cells. The aim of this study is to evaluate the efficiency of PLGA/ bPEI nanoparticles in transfection and virus production. Using a modified method of producing PLGA nanoparticles, PLGA/bPEI-DNA nanoparticles were examined for transfection efficiency and virus production yield in comparison with PLGA-DNA, bPEI-DNA nanoparticles or liposome-DNA complexes. After testing various ratios of PLGA, bPEI, and DNA, the ratio of 6:3:1 (PLGA:bPEI:DNA, w/w/w) was determined to be optimal, with acceptable cellular toxicity. PLGA/bPEI-DNA (6:3:1) nanoparticles showed superior transfection efficiency, especially in multiple gene transfection, and viral yield when compared with liposome-DNA complexes. The culture supernatants of HEK293FT cells transfected with PLGA/bPEI-DNA of viral constructs containing reprogramming factors (Oct4, Sox2, Klf4, or c-Myc) successfully and more efficiently generated induced pluripotent stem cell colonies from mouse embryonic fibroblasts. These results strongly suggest that PLGA/bPEI-DNA nanoparticles can provide significant advantages in studying the effect of multiple factor delivery such as in reprogramming or direct conversion of cell fate.
Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L
Eun-Hwa Sohn, Seon-A Jang, Haemi Joo, Sulkyoung Park, Se-Chan Kang, Chul-Hoon Lee, Sun-Young Kim
Clinical and Molecular Allergy , 2011, DOI: 10.1186/1476-7961-9-4
Abstract: This study examined the effect of ALBE on the release of β-hexosaminidase in antigen-stimulated-RBL-2H3 cells. We also evaluated the ConA-induced expression of IL-4, IL-5, mitogen-activated protein kinases (MAPKs), and nuclear factor (NF)-κB using RT-PCR, Western blotting, and ELISA in mouse splenocytes after ALBE treatment.We observed significant inhibition of β-hexosaminidase release in RBL-2H3 cells and suppressed mRNA expression and protein secretion of IL-4 and IL-5 induced by ConA-treated primary murine splenocytes after ALBE treatment. Additionally, ALBE (100 μg/mL) suppressed not only the transcriptional activation of NF-κB, but also the phosphorylation of MAPKs in ConA-treated primary splenocytes.These results suggest that ALBE inhibits the expression of IL-4 and IL-5 by downregulating MAPKs and NF-κB activation in ConA-treated splenocytes and supports the hypothesis that ALBE may have beneficial effects in the treatment of allergic diseases, including atopic dermatitis.Atopic dermatitis is a chronic, allergic inflammatory skin disorder characterized by pruritic chronic eczema, elevated serum IgE levels, and massive cellular infiltrates, including eosinophils, mast cells, and lymphocytes [1,2]. Because mast cells play essential roles in provoking the pathogenesis of allergic reactions via the degranulation process, measuring the degree of degranulation reflects the level of mast cell activation. β-Hexosaminidase released by these cells during this process has been reported to be a suitable marker for determining the degree of degranulation [3]. After an allergen triggers the allergic reactions, allergic mediators, including histamine, cytokines, and arachidonic acid derivatives, provoke acute and chronic allergic inflammation responses [4,5]. Various cells involved in the allergic reaction infiltrate the lesion. Among these, T helper 2 (Th2) cells are the most important cell type involved in atopic dermatitis development. Th2 cells release cytokines, such a
Pancreatic Lipase Inhibition by C-Glycosidic Flavones Isolated from Eremochloa ophiuroides
Eun Mi Lee,Seung Sik Lee,Byung Yeoup Chung,Jae-Young Cho,In Chul Lee,So Ra Ahn,Soo Jeung Jang,Tae Hoon Kim
Molecules , 2010, DOI: 10.3390/molecules15118251
Abstract: Activity-guided fractionation of a methanolic extract of the leaves of Eremochloa ophiuroides (centipede grass) using a pancreatic lipase inhibitory assay led to the isolation and identification of a new C-glycosidic flavone, luteolin 6-C-b-D-boivinopyranoside (1), as well as eight known compounds. The structures of these compounds were established on the basis of interpretation of their spectroscopic data. Among these isolates, the C-glycosidic flavones 1–5 showed potent inhibitory effects on pancreatic lipase, with IC50 values ranging from 18.5 ± 2.6 to 50.5 ± 3.9 mM.
Natural Killer Cells from Patients with Chronic Rhinosinusitis Have Impaired Effector Functions
Ji Heui Kim, Gye Eun Kim, Gye Song Cho, Hyung-Joon Kwon, Chul Hyun Joo, Hun Sik Kim, Yong Ju Jang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0077177
Abstract: Natural killer (NK) cells are multicompetent lymphocytes of the innate immune system that play a central role in host defense and immune regulation. Although increasing evidence suggests that innate immunity plays a key role in the pathogenesis of chronic rhinosinusitis (CRS), the role of NK cells in CRS has been poorly studied. This study aimed to characterize the peripheral blood NK cells from patients with CRS, and to compare the functions of these cells with those from non-CRS controls. The correlation between NK cell functional activity and prognosis was also assessed. Eighteen CRS patients and 19 healthy non-CRS controls were included. The patients with CRS were classified into two subgroups, namely a treatment-responsive group and recalcitrant group. NK cell degranulation was determined by measuring the cell surface expression of CD107a against 721.221 and K562 cells. Intracytoplasmic cytokine production was determined by flow cytometry. Compared to the controls, the NK cells of CRS group had an impaired ability to degranulate and to produce cytokines such as IFN-γ and TNF-α. The recalcitrant subgroup showed the most severe defects in NK cell effector functions. Moreover, the decreased NK cell functions in patients with CRS were associated with poor prognostic factors such as concomitant asthma and peripheral blood eosinophilia. NK cells, which were originally named for their ability to mediate spontaneous cytotoxicity towards diseased cells including infected cells, may play an important role in regulating the inflammatory process in CRS pathogenesis.
ABCD2 Is a Direct Target of β-Catenin and TCF-4: Implications for X-Linked Adrenoleukodystrophy Therapy
Chul-Yong Park, Han-Soo Kim, Jiho Jang, Hyunji Lee, Jae Souk Lee, Jeong-Eun Yoo, Dongjin R. Lee, Dong-Wook Kim
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0056242
Abstract: X-linked adrenoleukodystrophy (X-ALD) is a peroxisomal disorder caused by mutations in the ABCD1 gene that encodes the peroxisomal ATP-binding cassette (ABC) transporter subfamily D member 1 protein (ABCD1), which is referred to as the adrenoleukodystrophy protein (ALDP). Induction of the ABCD2 gene, the closest homolog of ABCD1, has been mentioned as a possible therapeutic option for the defective ABCD1 protein in X-ALD. However, little is known about the transcriptional regulation of ABCD2 gene expression. Here, through in silico analysis, we found two putative TCF-4 binding elements between nucleotide positions ?360 and ?260 of the promoter region of the ABCD2 gene. The transcriptional activity of the ABCD2 promoter was strongly increased by ectopic expression of β-catenin and TCF-4. In addition, mutation of either or both TCF-4 binding elements by site-directed mutagenesis decreased promoter activity. This was further validated by the finding that β-catenin and the promoter of the ABCD2 gene were pulled down with a β-catenin antibody in a chromatin immunoprecipitation assay. Moreover, real-time PCR analysis revealed that β-catenin and TCF-4 increased mRNA levels of ABCD2 in both a hepatocellular carcinoma cell line and primary fibroblasts from an X-ALD patient. Interestingly, we found that the levels of very long chain fatty acids were decreased by ectopic expression of ABCD2-GFP as well as β-catenin and TCF-4. Taken together, our results demonstrate for the first time the direct regulation of ABCD2 by β-catenin and TCF-4.
Anti-Diarrheal Effects of a Combination of Korean Traditional Herbal Extracts and Dioctahedral Smectite on Piglet Diarrhea Caused by Escherichia coli and Salmonella typhimurium
Won-Chul Jung§, Chun-Nam Cha§1, Yeo-Eun Lee2, Chang-Yeol Yoo2, 3, Eun-Kee Park4, Suk Kim and Hu-Jang Lee*
Pakistan Veterinary Journal , 2011,
Abstract: The present study evaluated anti-diarrheal effects of a mixture of Coptidis rhizoma, Lonicerae flos, and Paeonia japonica (1:1:1, v/v/v) methanol extracts and dioctahedral smectite (CLPD) on piglet diarrhea caused by Escherichia coli (E. coli) and Salmonella typhimurium (S. typhimurium). Diarrhea index of group 1 administered by 0.5% CLPD mixed with feed, decreased with the passage of time and was insignificantly differed compared to that of control. In group 2 administered by 1.0% CLPD mixed with feed, diarrhea index was significantly decreased compared to that of control and group I during overall experimental periods (P<0.05). After administration of CLPD mixed with feed, the number of E. coli and S. typhimurium in piglet feces of group 1 except for the 1st day was significantly decreased compared to that of the control group (P<0.05), and the number of E. coli and S. typhimurium in piglet feces of group 2 except for the 1st day was significantly decreased compared to that of the control group and group I (P<0.05). This study showed that CLPD had anti-diarrheal effect on E. coli and S. typhimurium causing diarrhea in piglets. CLPD could be an effective candidate for the treatment of enteric bacterial infections in piglets.
Therapeutic Effects of Autologous Tumor-Derived Nanovesicles on Melanoma Growth and Metastasis
Eun-Young Lee, Kyong-Su Park, Yae Jin Yoon, Jaewook Lee, Hyung-Geun Moon, Su Chul Jang, Kyoung-Ho Choi, Yoon-Keun Kim, Yong Song Gho
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0033330
Abstract: Cancer vaccines with optimal tumor-associated antigens show promise for anti-tumor immunotherapy. Recently, nano-sized vesicles, such as exosomes derived from tumors, were suggested as potential antigen candidates, although the total yield of exosomes is not sufficient for clinical applications. In the present study, we developed a new vaccine strategy based on nano-sized vesicles derived from primary autologous tumors. Through homogenization and sonication of tumor tissues, we achieved high yields of vesicle-bound antigens. These nanovesicles were enriched with antigenic membrane targets but lacked nuclear autoantigens. Furthermore, these nanovesicles together with adjuvant activated dendritic cells in vitro, and induced effective anti-tumor immune responses in both primary and metastatic melanoma mouse models. Therefore, autologous tumor-derived nanovesicles may represent a novel source of antigens with high-level immunogenicity for use in acellular vaccines without compromising safety. Our strategy is cost-effective and can be applied to patient-specific cancer therapeutic vaccination.
Clinical implications of fatty pancreas: Correlations between fatty pancreas and metabolic syndrome
Jun Seok Lee, Sang Heum Kim, Dae Won Jun, Jee Hye Han, Eun Chul Jang, Ji Young Park, Byung Kwan Son, Seong Hwan Kim, Yoon Ju Jo, Young Sook Park, Yong Soo Kim
World Journal of Gastroenterology , 2009,
Abstract: AIM: To investigate the clinical implications of lipid deposition in the pancreas (fatty pancreas).METHODS: The subjects of this study were 293 patients who had undergone abdominal computed tomography (CT) and sonography. Fatty pancreas was diagnosed by sonographic findings and subdivided into mild, moderate, and severe fatty pancreas groups comparing to the retroperitoneal fat echogenicity.RESULTS: Fatty pancreas was associated with higher levels for visceral fat, waist circumference, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol, triglyceride, high density lipoprotein, free fatty acid, γ-GTP, insulin, and the homeostasis model assessment of insulin resistance (HOMA-IR) than the control group (P < 0.05). HOMA-IR, visceral fat, triglyceride, and ALT also tended to increase with the degree of fat deposition in the pancreas on sonography. In a multivariate logistic regression analysis, HOMA-IR, visceral fat, and ALT level were independently related to fatty pancreas after adjustment for age, body mass index, and lipid profile. The incidence of metabolic syndrome in the fatty pancreas group was significantly higher than in the control group, and the numbers of metabolic syndrome parameters were significantly higher in the fatty pancreas group (P < 0.05).CONCLUSION: Sonographic fatty pancrease showed higher insulin resistance, visceral fat area, triglyceride, and ALT levels than normal pancreases. Fatty pancreas also showed a strong correlation with metabolic syndrome.
Cough in the Elderly Population: Relationships with Multiple Comorbidity
Woo-Jung Song, Alyn H. Morice, Min-Hye Kim, Seung-Eun Lee, Eun-Jung Jo, Sang-Min Lee, Ji-Won Han, Tae Hui Kim, Sae-Hoon Kim, Hak-Chul Jang, Ki Woong Kim, Sang-Heon Cho, Kyung-Up Min, Yoon-Seok Chang
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0078081
Abstract: Background The epidemiology of cough in the elderly population has not been studied comprehensively. The present study aimed to investigate the epidemiology of cough in a community elderly population, particularly in relation with their comorbidity. Methods A cross-sectional analysis was performed using a baseline dataset from the Korean Longitudinal Study on Health and Aging, a community-based elderly population cohort study. Three types of cough (frequent cough, chronic persistent cough, and nocturnal cough) were defined using questionnaires. Comorbidity was examined using a structured questionnaire. Health-related quality of life was assessed using the Short Form 36 questionnaire. Results The prevalence was 9.3% for frequent cough, 4.6% for chronic persistent cough, and 7.3% for nocturnal cough. In multivariate logistic regression analyses, smoking, asthma and allergic rhinitis were found to be risk factors for cough in the elderly. Interestingly, among comorbidities, constipation and uncontrolled diabetes mellitus (HbA1c ≥ 8%) were also found to have positive associations with elderly cough. In the Short Form 36 scores, chronic persistent cough was independently related to impairment of quality of life, predominantly in the mental component. Conclusions Cough has a high prevalence and is detrimental to quality of life in the elderly. Associations with smoking, asthma and rhinitis confirmed previous findings in younger populations. Previously unrecognised relationships with constipation and uncontrolled diabetes mellitus suggested the multi-faceted nature of cough in the elderly.
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