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Search Results: 1 - 10 of 3486 matches for " Eui-Sic Cho "
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Fibroblast Growth Factor-4 Enhances Proliferation of Mouse Embryonic Stem Cells via Activation of c-Jun Signaling
Sung-Ho Kook, Young-Mi Jeon, Shin-Saeng Lim, Moon-Ju Jang, Eui-Sic Cho, Seung-Yeop Lee, Ki-Choon Choi, Jong-Ghee Kim, Jeong-Chae Lee
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0071641
Abstract: Fibroblast growth factor-4 (FGF4) is expressed in embryonic stages and in adult tissues, where it plays critical roles in modulating multiple cellular functions. However, the exact roles of FGF4 on proliferation and differentiation of embryonic stem cells (ESCs) are not completely understood. Exogenous addition of FGF4 stimulated proliferation of mouse ESCs (mESCs), as proven by the increases in DNA synthesis and cell cycle regulatory protein induction. These increases were almost completely inhibited by pre-treating cells with anti-FGF4 antibody. FGF4 also activated c-Jun N-terminal kinase (JNK) and extracellular-signal regulated kinase (ERK) signaling, but not p38 kinase. Blockage of JNK signaling by SP600125 or by transfection with its specific siRNA significantly inhibited FGF4-stimulated cell proliferation through the suppression of c-Jun induction and activator protein-1 (AP-1) activity. However, ERK or p38 kinase inhibitor did not affect FGF4-stimulated proliferation in mESCs. FGF4 suppressed osteogenic differentiation of mESCs by inhibiting expression of transcription factors involved in bone formation. Further, exogenous FGF4 addition stimulated proliferation of human periodontal ligament stem cells (hPDLSCs) and bone marrow mesenchymal stem cells (BMMSCs) via activation of ERK signaling. FGF4 also augmented mineralization of hPDLSCs, but not of BMMSCs. Collectively, it is suggested that FGF4 triggers proliferation of stem cells by activating MAPK-mediated signaling, while it affects differently osteogenic differentiation according to the origins of stem cells.
Crosstalk between Nuclear Factor I-C and Transforming Growth Factor-β1 Signaling Regulates Odontoblast Differentiation and Homeostasis
Dong-Seol Lee, Won-Joon Yoon, Eui Sic Cho, Heung-Joong Kim, Richard M. Gronostajski, Moon-Il Cho, Joo-Cheol Park
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0029160
Abstract: Transforming growth factor-β1 (TGF-β1) signaling plays a key role in vertebrate development, homeostasis, and disease. Nuclear factor I-C (NFI-C) has been implicated in TGF-β1 signaling, extracellular matrix gene transcription, and tooth root development. However, the functional relationship between NFI-C and TGF-β1 signaling remains uncharacterized. The purpose of this study was to identify the molecular interactions between NFI-C and TGF-β1 signaling in mouse odontoblasts. Real-time polymerase chain reaction and western analysis demonstrated that NFI-C expression levels were inversely proportional to levels of TGF-β1 signaling molecules during in vitro odontoblast differentiation. Western blot and immunofluorescence results showed that NFI-C was significantly degraded after TGF-β1 addition in odontoblasts, and the formation of the Smad3 complex was essential for NFI-C degradation. Additionally, ubiquitination assay results showed that Smurf1 and Smurf2 induced NFI-C degradation and polyubiquitination in a TGF-β1-dependent manner. Both kinase and in vitro binding assays revealed that the interaction between NFI-C and Smurf1/Smurf2 requires the activation of the mitogen-activated protein kinase pathway by TGF-β1. Moreover, degradation of NFI-C induced by TGF-β1 occurred generally in cell types other than odontoblasts in normal human breast epithelial cells. In contrast, NFI-C induced dephosphorylation of p-Smad2/3. These results show that crosstalk between NFI-C and TGF-β1 signaling regulates cell differentiation and homeostatic processes in odontoblasts, which might constitute a common cellular mechanism.
Controllability of Touch-screen Phones based on Various Grip Postures
Youngjae Im,Sungil Cho,Sungjoon Park,Eui S. Jung
Lecture Notes in Engineering and Computer Science , 2010,
Seebeck effect at the atomic scale
Eui-Sup Lee,Sanghee Cho,Ho-Ki Lyeo,Yong-Hyun Kim
Physics , 2013, DOI: 10.1103/PhysRevLett.112.136601
Abstract: The atomic variations of electronic wavefunctions at the surface and electron scattering near a defect have been detected unprecedentedly by tracing thermoelectric voltages given a temperature bias [Cho et al., Nature Mater. 12, 913 (2013)]. Because thermoelectricity, or Seebeck effect, is associated with heat-induced electron diffusion, how the thermoelectric signal is related to the atomic-scale wavefunctions and what the role of the temperature is at such a length scale remain very unclear. Here we show that coherent electron and heat transport through a point-like contact produces an atomic Seebeck effect, which is described by mesoscopic Seebeck coefficient multiplied with an effective temperature drop at the interface. The mesoscopic Seebeck coefficient is approximately proportional to the logarithmic energy derivative of local density of states at the Fermi energy. We deduced that the effective temperature drop at the tip-sample junction could vary at a sub-angstrom scale depending on atom-to-atom interaction at the interface. A computer-based simulation method of thermoelectric images is proposed, and a point defect in graphene was identified by comparing experiment and the simulation of thermoelectric imaging.
Colorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells
Bok Hong, Ji-Hoon Cho, Hyunjung Kim, Eun-Jeong Choi, Sangchul Rho, Jongmin Kim, Ji Kim, Dong-Sic Choi, Yoon-Keun Kim, Daehee Hwang, Yong Gho
BMC Genomics , 2009, DOI: 10.1186/1471-2164-10-556
Abstract: We present potential roles of CRC cell-derived microvesicles in tumor progression via a global comparative microvesicular and cellular transcriptomic analysis of human SW480 CRC cells. We first identified 11,327 microvesicular mRNAs involved in tumorigenesis-related processes that reflect the physiology of donor CRC cells. We then found 241 mRNAs enriched in the microvesicles above donor cell levels, of which 27 were involved in cell cycle-related processes. Network analysis revealed that most of the cell cycle-related microvesicle-enriched mRNAs were associated with M-phase activities. The integration of two mRNA datasets showed that these M-phase-related mRNAs were differentially regulated across CRC patients, suggesting their potential roles in tumor progression. Finally, we experimentally verified the network-driven hypothesis by showing a significant increase in proliferation of endothelial cells treated with the microvesicles.Our study demonstrates that CRC cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells, suggesting that microvesicles of cancer cells can be involved in tumor growth and metastasis by facilitating angiogenesis-related processes. This information will help elucidate the pathophysiological functions of tumor-derived microvesicles, and aid in the development of cancer diagnostics, including colorectal cancer.During growth or activation, a variety of cell types, including hematopoietic, epithelial, and tumor cells, shed small membrane vesicles called microvesicles (exosomes) [1-6]. These microvesicles, which are 30-200 nm in diameter, are derived from the endosomal membrane compartment following the fusion of multivesicular bodies with the plasma membrane. Cells also release microvesicles directly by the outward budding of the plasma membrane in a calcium-dependent manner [4]. Microvesicles have been also found in various body fluids, such as plasma, malignant pleural effusion, and
Molecular Mechanism of Macrophage Activation by Red Ginseng Acidic Polysaccharide from Korean Red Ginseng
Se Eun Byeon,Jaehwi Lee,Ji Hye Kim,Woo Seok Yang,Yi-Seong Kwak,Sun Young Kim,Eui Su Choung,Man Hee Rhee,Jae Youl Cho
Mediators of Inflammation , 2012, DOI: 10.1155/2012/732860
Abstract: Red ginseng acidic polysaccharide (RGAP), isolated from Korean red ginseng, displays immunostimulatory and antitumor activities. Even though numerous studies have been reported, the mechanism as to how RGAP is able to stimulate the immune response is not clear. In this study, we aimed to explore the mechanism of molecular activation of RGAP in macrophages. RGAP treatment strongly induced NO production in RAW264.7 cells without altering morphological changes, although the activity was not strong compared to LPS-induced dendritic-like morphology in RAW264.7 cells. RGAP-induced NO production was accompanied with enhanced mRNA levels of iNOS and increases in nuclear transcription factors such as NF-κB, AP-1, STAT-1, ATF-2, and CREB. According to pharmacological evaluation with specific enzyme inhibitors, Western blot analysis of intracellular signaling proteins and inhibitory pattern using blocking antibodies, ERK, and JNK were found to be the most important signaling enzymes compared to LPS signaling cascade. Further, TLR2 seems to be a target surface receptor of RGAP. Lastly, macrophages isolated from RGS2 knockout mice or wortmannin exposure strongly upregulated RGAP-treated NO production. Therefore, our results suggest that RGAP can activate macrophage function through activation of transcription factors such as NF-κB and AP-1 and their upstream signaling enzymes such as ERK and JNK.
How Current Clinical Practice Guidelines for Low Back Pain Reflect Traditional Medicine in East Asian Countries: A Systematic Review of Clinical Practice Guidelines and Systematic Reviews
Hyun-Woo Cho, Eui-Hyoung Hwang, Byungmook Lim, Kwang-Ho Heo, Jian-Ping Liu, Kiichiro Tsutani, Myeong Soo Lee, Byung-Cheul Shin
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0088027
Abstract: Objectives The aims of this study were to investigate whether there is a gap between evidence of traditional medicine (TM) interventions in East-Asian countries from the current Clinical Practice Guidelines (CPGs) and evidence from current systematic reviews and meta-analyses (SR-MAs) and to analyze the impact of this gap on present CPGs. Methods We examined 5 representative TM interventions in the health care systems of East-Asian countries. We searched seven relevant databases for CPGs to identify whether core CPGs included evidence of TM interventions, and we searched 11 databases for SR-MAs to re-evaluate current evidence on TM interventions. We then compared the gap between the evidence from CPGs and SR-MAs. Results Thirteen CPGs and 22 SR-MAs met our inclusion criteria. Of the 13 CPGs, 7 CPGs (54%) mentioned TM interventions, and all were for acupuncture (only one was for both acupuncture and acupressure). However, the CPGs did not recommend acupuncture (or acupressure). Of 22 SR-MAs, 16 were for acupuncture, 5 for manual therapy, 1 for cupping, and none for moxibustion and herbal medicine. Comparing the evidence from CPGs and SR-MAs, an underestimation or omission of evidence for acupuncture, cupping, and manual therapy in current CPGs was detected. Thus, applying the results from the SR-MAs, we moderately recommend acupuncture for chronic LBP, but we inconclusively recommend acupuncture for (sub)acute LBP due to the limited current evidence. Furthermore, we weakly recommend cupping and manual therapy for both (sub)acute and chronic LBP. We cannot provide recommendations for moxibustion and herbal medicine due to a lack of evidence. Conclusions The current CPGs did not fully reflect the evidence for TM interventions. As relevant studies such as SR-MAs are conducted and evidence increases, the current evidence on acupuncture, cupping, and manual therapy should be rigorously considered in the process of developing or updating the CPG system.
Interleukin-1α and tumor necrosis factor-α expression on the compressed side of gingiva during orthodontic tooth movement  [PDF]
Hyoung-Seon Baik, Chong-Kwan Kim, Won Hee Lim, Youn Sic Chun
Open Journal of Stomatology (OJST) , 2012, DOI: 10.4236/ojst.2012.23033
Abstract: Objectives: The aim of this study was to investigate the effect of orthodontic loading on the expression of interleukin-1α (IL-1α) and tumor necrosis factor-α (TNF-α) in compressed gingiva. Materials and Methods: Twenty-four male Wistar rats were used with four rats as controls at day 0. In ten rats, corticotomy was performed on either left or right side and the re-maining side was served as control, and killed at 7 and 14 days. In the remaining ten rats, maxillary right and left first molars were moved orthodontically with a constant force of 20 g; appliance and appliance in conjunction with cortictomy (appliance-corticotomy) were sequentially alternated between left and right sides and euthanized at 7 and 14 days. Real time polymerase chain reactions of compressed gingiva excised from the euthanized rats was performed to measure mRNA expressions of IL-1α and TNF-α. Results: There were no significant differences in the expression of IL-1α between all the groups. On the other hand, TNF-α from the pressure side in the ap- pliance group and the appliance in conjunction with corticotomy group showed significant increase on the 7th day compared to that of either control or corticotomy group. Conclusions: Orthodontic loading induced the elevation of TNF-α in compressed gingiva
Expression of MMP-9 and -13 on the Pressure Side under Orthodontic Loading  [PDF]
Su-Jung Mah, Yoonjung Lee, Youn-Sic Chun, Won Hee Lim
Open Journal of Stomatology (OJST) , 2014, DOI: 10.4236/ojst.2014.48055
Abstract: Purpose: The purpose of this study was to investigate the expression of MMP-9 and MMP-13 on compression side of tooth movement. Material and Methods: Twenty male Wistar rats were used for this experiment. Maxillary right first molar was moved orthodontically with a constant force of 20 g and on the side of the maxillary left first molar was used as control group. On the 4th, 10th and 17th days after experiment, 5 rats were euthanized, respectively. Histologic process was processed for immunohistochemical staining of MMP-9 and -13. Results: MMP-9 and -13 expressions were upregulated at day 4 after tooth movement. The expression of MMP-9 was not observed at days 7, 10 and 14, while the expression of MMP-13 was greatly increased at days 7 and 10. Conclusion: This study suggests that different characteristics of MMPs expression may contribute to the remodeling process of collagen fibers in the PDL during tooth movement.
Lower bounds of the Dirac eigenvalues on compact Riemannian spin manifolds with locally product structure
Eui Chul Kim
Mathematics , 2004,
Abstract: We study some similarities between almost product Riemannian structures and almost Hermitian structures. Inspired by the similarities, we prove lower eigenvalue estimates for the Dirac operator on compact Riemannian spin manifolds with locally product structures. We also provide some examples (limiting manifolds) for the limiting case of the estimates.
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