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Search Results: 1 - 10 of 22405 matches for " Estevao Souza "
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New insights about host response to smallpox using microarray data
Gustavo H Esteves, Ana CQ Simoes, Estevao Souza, Rodrigo A Dias, Raydonal Ospina, Thiago M Venancio
BMC Systems Biology , 2007, DOI: 10.1186/1752-0509-1-38
Abstract: We used KEGG pathways annotations to define groups of genes (or modules), and subsequently compared them to macaque survival times. This technique provided additional insights about the host response to this disease, such as increased expression of the cytokines and ECM receptors in the individuals with higher survival times. These results could indicate that these gene groups could influence an effective response from the host to smallpox.Macaques with higher survival times clearly express some specific pathways previously unidentified using regular gene-by-gene approaches. Our work also shows how third party analysis of public datasets can be important to support new hypotheses to relevant biological problems.Large scale gene expression analysis with microarray technology is expanding and generating a large amount of high quality, publicly available data. In the present work we analyzed a dataset derived from monkeys infected by smallpox, published by Rubins et al [1]. Smallpox is a lethal disease that was endemic in many parts of the world until eradicated by a massive immunization program developed by the World Health Organization. Its fatality rate was estimated to be 30%, and the survivors often had disfiguring scars [2].There are serious concerns about the use of smallpox as a bioweapon [3,4]. Recently, some health care workers were vaccinated by the UK government for the analysis of antibody responses [5]. Pox viruses display unique abilities to interfere with the host immune system, producing immune modulators [6] and there are at least 16 viral genes involved in combating the host immune response [7]. The original study's goal was to analyze the evolution of the gene expression of the peripheral blood cells of variola-infected monkeys, so as to clarify the biological processes associated with host-pathogen interactions [1].Among the important results was the absence of a TNF-α/NF-κB-activated transcriptional mechanism during systemic infection, which could
Controles microbiológicos y puntos de control en una planta elaboradora de filet de merluza para exportación
Estevao Belchior,Silvia; Pucci,Oscar Héctor;
Archivos Latinoamericanos de Nutrición , 2000,
Abstract: microbiological controls and critical control points during control "for export" hake fillets manufacturing process. indicator and food borne pathogens microorganisms in the "for export" hake fillets manufacturing were investigated in this study. critical control points were identified and prevention activities and control were proposed during seafood elaboration process. 45 samples of hake from sequential processing operation stages, 15 ice samples and 12 water samples from utensil washing, were collected. the samples were analyzed for their content of aerobic mesophilic bacteria, psychrotrophic bacteria, enterobacteria, total and fecal coliform bacteria, staphylococcus aureus and the presence of escherichia coli, salmonella and shigella. the analysis of the samples collected from the factory revealed that the amounts of aerobic mesophiles bacteria increased during manual filleting and packaging, in comparison with raw material. psychrotrophic bacteria were the predominant microorganisms, specially in hake samples. in addition, high levels of enterobacteria, which do not occur normally in fish, were detected in raw hake samples. staphylococcus aureus, escherichia coli, salmonella and shigella, were not isolated from any samples in this study. the goal of this work is to establish microbiological risks in the hake fillets manufacturing process and, therefore to make possible corrective and control actions to assure the quality and safety of seafood.
Diagnóstico de pseudotuberculosis en ovinos patagónicos Diagnosis of caseous lymphadenitis in sheep from Patagonia
S. Estevao Belchior,A. Gallardoz,A. ábalos,Y. Díaz
Revista argentina de microbiolog?-a , 2007,
Abstract: La linfadenitis caseosa (LAC) es una enfermedad bacteriana supurativa crónica que afecta a ovinos. El agente etiológico es Corynebacterium pseudotuberculosis. El diagnóstico diferencial con otras afecciones que presentan manifestaciones clínicas similares sólo puede hacerse sobre la base del aislamiento y la identificación del agente etiológico. El objetivo de este trabajo fue caracterizar metabólica y genéticamente al agente causal de abscesos granulomatosos observados en ovinos en la región patagónica. En las muestras, se observó un contenido caseoso rodeado de una membrana fibrosa, y en el examen histopatológico, un centro de necrosis caseosa rodeado por células epitelioides, linfocitos y polinucleares. Mediante estudios microscópicos, bacteriológicos y moleculares fue confirmada la infección causada por C. pseudotuberculosis biovar ovis. Caseous lymphadenitis (CLA) is a chronic bacterial, infectious and contagious disease caused by Corynebacterium pseudotuberculosis. It affects sheep and results in abscesses of the lymph nodes in subcutaneous tissue, as well as in internal organs such as lungs, liver and kidneys. Differential diagnosis of the disease is based on the isolation and biochemical identification of the etiological agent. The purpose of this study was to characterize the bacteria isolated from typical CLA lesions in sheep from Patagonia, Argentina, at metabolic and genetic levels. Macroscopic observations show a fibrous membrane containing caseous necrotic tissue. Histopathological analysis shows an eosinophilic necrotic area surrounded by epitheloid cells and polymorphonuclear infiltration. Other analyses performed such as microscopic observations, in vitro culture, biochemical tests and 16s rDNA sequencing confirmed diagnosis of caseous lymphadenitis due to C. pseudotuberculosis.
Caracterización de polaciones microbianas presentes en la macroalga comestible Monostroma undulatum, Wittrock
Gallardo,Adriana Alicia; Risso,Susana; Fajardo,María Angélica; Estevao Belchior,Silvia;
Archivos Latinoamericanos de Nutrición , 2004,
Abstract: characterization of microbial population present in the edible seaweed, monostroma undulatum, wittrock. the microbiological quality of monostroma undulatum, wittrock from the southern argentinean coast, was studied for its application for human food. also the diversity and function of the native bacterial population to this green seaweed was analyzed. samples were collected in puerto deseado, province of santa cruz, southern argentina (47o 45?l.s., 65o 55?l.w). the samples were analyzed for the presence of psycotrophic heterotrophic bacteria, marine heterotrophic bacteria, low nutritional request bacteria (lnrb), marine low nutritional request bacteria (lnrb marine), vibrio spp, total and thermotolerant colifom bacteria, anaerobic sulfite reducing bacteria, yeasts and moulds. the isolates were identified using standard techniques based on morphologic, physiologic and metabolic characteristics. among the gram-negative bacteria isolated, the predominant genera belonged to vibrio (20%), e. coli inactiva (18%), flavobacterium (11%), flexibacter (9%), moraxella (9%), alcaligenes/pseudomonas group (9%), aeromonas (2%), acinetobacter (2%). cotophaga (2%), photobacterium (2%), ps/caulobacter/alteromonas/spirillum group (2), the main genus of gram-positive bacteria was staphylococcus. were not detected human pathogenic bacteria. focai contamination indicator bacteria were not isolated from fresh seaweed and seawater. these results showed an adequate microbilogical quality of seaweed acceptable for human food. the bacterial population associated to monostroma undulatum, consisted of gram-negative, marine and psycotrophic microorganisms, including vibrios and enterobacteria as their main components. also the identified bacteria showed a great capacity to hydrolyze different substrates and so they might contribute to the balance of this marine ecosystem.
Desarrollo de una prueba de ELISA para detectar anticuerpos en carneros vacunados o infectados con Corynebacterium pseudotuberculosis
Solanet,Juan J.; Malena,Rosana; Estein,Silvia M.; Estevao Belchior,Silvia; Paolicchi,Fernando A.;
Revista argentina de microbiolog?-a , 2011,
Abstract: the aim of this study was to evaluate an indirect specific elisa developed for the detection of humoral immune response in vaccinated sheep and/or challenged with a corynebacterium pseudotuberculosis strain. healthy 4 month-old lambs were distributed into 4 groups: group 1 immunized (g1, n = 5), group 2 vaccinated/inoculated (g2, n = 8), group 3 inoculated (g3, n = 2) and group 4 control (g4, n = 2). groups g1 and g2 received two doses of an experimental bacterin. four weeks postvaccination, g2 and g3 groups were challenged with a c. pseudotuberculosis strain. serological titers were studied by elisa for 7 months and pathological studies were performed in groups g2, g3 and g4 by taking lung and lymph node samples for bacteriology and histopathology. the inoculated strain in g2 and g3 animals reproduced the macroscopic and microscopic lesions typical of caseous lymphadenitis (cl) and was isolated from the inoculation site, lymph nodes and/or lung in 7/8 animals from g2, and 2/2 animals of g3. the developed elisa test had sensitivity and specificity of 98% and 100% respectively, detected significant differences between serological reactors of different experimental groups and allowed to establish a relationship with the type of treatment. we conclude that the developed elisa may be a useful tool to identify infected animals with positive clinical cl.
Desarrollo de una prueba de ELISA para detectar anticuerpos en carneros vacunados o infectados con Corynebacterium pseudotuberculosis Development of an ELISA test to detect antibodies in vaccinated sheep or infected Corynebacterium pseudotuberculosis
Juan J. Solanet,Rosana Malena,Silvia M. Estein,Silvia Estevao Belchior
Revista argentina de microbiolog?-a , 2011,
Abstract: El objetivo de este trabajo fue evaluar un ELISA indirecto desarrollado para medir la respuesta inmune humoral en carneros vacunados contra la linfoadenitis caseosa (LC) y/o desafiados con una cepa de Corynebacterium pseudotuberculosis homóloga. Se distribuyeron corderos de 4 meses clínicamente sanos en 4 grupos: grupo 1, corderos vacunados (G1, n = 5); grupo 2, corderos vacunados e inoculados (G2, n = 8); grupo 3, corderos inoculados (G3, n = 2); y grupo 4, control (G4, n = 2). Los animales del G1 y del G2 recibieron dos dosis de una bacterina experimental; los del G2 y del G3 fueron desafiados con una cepa de C. pseudotuberculosis cuatro semanas posvacunación. Se estudiaron por ELISA los títulos serológicos durante 7 meses y se efectuaron las necropsias en los grupos G2, G3 y G4. Se tomaron muestras de pulmón y linfonódulos para efectuar estudios bacteriológicos e histopatológicos. La cepa inoculada en los animales del G2 y del G3 reprodujo las lesiones macroscópicas y microscópicas típicas de la LC; ésta fue aislada del sitio de inoculación, de linfonódulos o de pulmón en 7/8 animales del G2 y en 2/2 animales del G3. La prueba de ELISA, con una sensibilidad del 98% y una especificidad del 100%, detectó diferencias significativas entre los serorreactores de los diferentes grupos experimentales y permitió establecer una relación con el tipo de tratamiento aplicado. Se concluye que el ELISA desarrollado puede ser una herramienta útil para identificar animales infectados y con clínica positiva a la LC. The aim of this study was to evaluate an indirect specific ELISA developed for the detection of humoral immune response in vaccinated sheep and/or challenged with a Corynebacterium pseudotuberculosis strain. Healthy 4 month-old lambs were distributed into 4 groups: Group 1 immunized (G1, n = 5), Group 2 vaccinated/inoculated (G2, n = 8), Group 3 inoculated (G3, n = 2) and Group 4 control (G4, n = 2). Groups G1 and G2 received two doses of an experimental bacterin. Four weeks postvaccination, G2 and G3 groups were challenged with a C. pseudotuberculosis strain. Serological titers were studied by ELISA for 7 months and pathological studies were performed in groups G2, G3 and G4 by taking lung and lymph node samples for bacteriology and histopathology. The inoculated strain in G2 and G3 animals reproduced the macroscopic and microscopic lesions typical of caseous lymphadenitis (CL) and was isolated from the inoculation site, lymph nodes and/or lung in 7/8 animals from G2, and 2/2 animals of G3. The developed ELISA test had sensitivity and specificity of 98% a
Contenido en algunos nutrientes del alga marina comestible, Monostroma undulatum, Wittrock de la costa patogónica argentina
Risso,Susana; Escudero,Carlos; Estevao Belchior,Silvia; de Portela4,María Luz; Fajardo,María Angélica;
Archivos Latinoamericanos de Nutrición , 2003,
Abstract: summary: chemical composition and seasonal fluctuations of the edible green seaweed, monostroma undulatum, wittrock, from the southern argentina coast. the chemical composition of green seaweed, monostroma undulatum, wittrock, growing in the southern argentine coast, was studied to obtain information regarding nutritional value. samples were collected in puerto deseado, province of santa cruz (47o 45?l.s., 65o 55?l.w.), from october to december 1999 and 2000. it has been analyzed six sample during this period. algae were washed with sea water and dried at room temperature for 24 hs. moisture, nitrogen, lipids and ashes were determined according to aoac; fiber (total, soluble and insoluble), according to lahaye. after mineralization with nitric acid, sodium and potassium were determined by flame photometry, calcium by complexometric method, and phosphorus by gomori?s method. the ranges expressed per 100 g dry algae were: protein (nx6,25): 12.89-21.85; ashes (g): 33.92-40.05; lipid (g): 0.32-1.47; total fiber (g): 14,36-19,6; digestible carbohydrates (calculated by difference) (g): 20.86-32.48; sodium (g): 7.39-13.11; potassium (g): 1.38-3.18; calcium (mg): 149-226; phosphorus (mg): 190-447; vitamin c (mg): 159-455. these results show that this green seaweed is an important source for protein, fiber, macronutrients minerals and vitamin c, during the macroscopic period. besides, there was an important fluctuation that must be taken into account to consider the commercial collection to use it in human nutrition.
Diagnóstico de pseudotuberculosis en ovinos patagónicos
Estevao Belchior,S.; Gallardoz,A.; ábalos,A.; Díaz,Y.; álvarez,L.; Callejo,R.; Prieto,M.; Jodor,N.; Jensen,O.;
Revista argentina de microbiolog?-a , 2007,
Abstract: caseous lymphadenitis (cla) is a chronic bacterial, infectious and contagious disease caused by corynebacterium pseudotuberculosis. it affects sheep and results in abscesses of the lymph nodes in subcutaneous tissue, as well as in internal organs such as lungs, liver and kidneys. differential diagnosis of the disease is based on the isolation and biochemical identification of the etiological agent. the purpose of this study was to characterize the bacteria isolated from typical cla lesions in sheep from patagonia, argentina, at metabolic and genetic levels. macroscopic observations show a fibrous membrane containing caseous necrotic tissue. histopathological analysis shows an eosinophilic necrotic area surrounded by epitheloid cells and polymorphonuclear infiltration. other analyses performed such as microscopic observations, in vitro culture, biochemical tests and 16s rdna sequencing confirmed diagnosis of caseous lymphadenitis due to c. pseudotuberculosis.
Morphological Analysis of Neonates of Rats Treated with Dexamethasone in the Initial Phase of Pregnancy Análisis Morfológico de Neonatos de Ratas Tratadas con Dexametasona en Fase Inicial de la Pre ez
Paulo Estevao Araújo Vila?a Júnior,Alvaro Aguiar Coelho Teixeira,Valéria Wanderley Teixeira,Eleonora de Figueiredo Moraes
International Journal of Morphology , 2008,
Abstract: The glucocorticoid dexamethasone has been largely used due to its anti-inflammatory effect. However, several authors report that the excessive exposition to it during pregnancy may cause a retard in the development in several tissues, mainly: liver, lungs and kidneys. But, the majority of the works are done with the application of dexamethasone in the late periods of pregnancy. Because of the lack of researches that evalúate the effects in the beginning of gestation, this paper aimed at evaluating the effect of dexamethasone administered in the initial phase of pregnancy, o ver the morphology of neonates rat. It was used 10 albino rats (Rattus norvegicus albinus) aged 90 days from the lineage Wistar. The female were coupled and divided in two groups: Group I - rats not submitted to the dexamethasone application (control); Group II - rats submitted to the dexamethasone application in the first 5 days of pregnancy. The results show that the treatment with dexamethasone in a dosage of 0.8mg/Kg during the 5 first days of pregnancy does not produces a weight and height reduction or malformation in the offspring, it does not cause changes in the development of the liver and kidneys of neonate rats, but it leads to a reduction in the denseness of the interalveolar septa causing a higher distensión of the alveoli. El glucocorticoide dexametasona ha sido ampliamente utilizado en virtud de su potencial antiinflamatorio. Sin embargo, varios autores relatan que la exposición excesiva a la dexametasona durante la pre ez puede causar el retardo del desarrollo de varios tejidos, principalmente hígado, pulmones y rí ones. La mayoría de los trabajos son llevados a cabo con la aplicación de dexametasona en los períodos tardíos de la gestación. El objetivo del trabajo fue evaluar el efecto de la dexametasona, sobre la morfología de ratones neonatos, administrada en la fase inicial de la pre ez. Fueron utilizadas 10 ratas Wistar albinas (Rattus norvegicus albinus) con 90 días de edad. Las hembras fueron apareadas y divididas en dos grupos: Grupo I- ratas no sometidas a la dexametasona (grupo control) y Grupo II - ratas sometidas a la aplicación de dexametasona durante los cinco primeros días de pre ez. Los resultados mostraron que el tratamiento con dexametasona en dosis de 0,8mg/Kg, a lo largo de los cinco primeros días de la pre ez, no produce reducción de peso, longitud o malformación en la prole, tampoco causa alteraciones en el desarrollo del hígado y rí ones en los ratones neonatos, pero sí reduce el grosor de los septos interalveolares, causando de esta manera, mayo
Morphological Analysis of Neonates of Rats Treated with Dexamethasone in the Initial Phase of Pregnancy
Vila?a Júnior,Paulo Estevao Araújo; Teixeira,Alvaro Aguiar Coelho; Wanderley Teixeira,Valéria; Moraes,Eleonora de Figueiredo; Araújo,Ana Claudia Carvalho de; Maia,Carina Scanoni;
International Journal of Morphology , 2008, DOI: 10.4067/S0717-95022008000300002
Abstract: the glucocorticoid dexamethasone has been largely used due to its anti-inflammatory effect. however, several authors report that the excessive exposition to it during pregnancy may cause a retard in the development in several tissues, mainly: liver, lungs and kidneys. but, the majority of the works are done with the application of dexamethasone in the late periods of pregnancy. because of the lack of researches that evalúate the effects in the beginning of gestation, this paper aimed at evaluating the effect of dexamethasone administered in the initial phase of pregnancy, o ver the morphology of neonates rat. it was used 10 albino rats (rattus norvegicus albinus) aged 90 days from the lineage wistar. the female were coupled and divided in two groups: group i - rats not submitted to the dexamethasone application (control); group ii - rats submitted to the dexamethasone application in the first 5 days of pregnancy. the results show that the treatment with dexamethasone in a dosage of 0.8mg/kg during the 5 first days of pregnancy does not produces a weight and height reduction or malformation in the offspring, it does not cause changes in the development of the liver and kidneys of neonate rats, but it leads to a reduction in the denseness of the interalveolar septa causing a higher distensión of the alveoli.
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