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Search Results: 1 - 10 of 13218 matches for " Eric Klett "
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Localization of ABCG5 and ABCG8 proteins in human liver, gall bladder and intestine
Eric L Klett, Mi-Hye Lee, David B Adams, Kenneth D Chavin, Shailendra B Patel
BMC Gastroenterology , 2004, DOI: 10.1186/1471-230x-4-21
Abstract: Here we report the biochemical and immuno-localization of ABCG5 and ABCG8 in human liver, gallbladder and intestine using cell fractionation and immunohistochemical analyses.We raised peptide antibodies against ABCG5 and ABCG8 proteins. Using human liver samples, cell fractionation studies showed both proteins are found in membrane fractions, but they did not co-localize with caveolin-rafts, ER, Golgi or mitochondrial markers. Although their distribution in the sub-fractions was similar, they were not completely contiguous. Immunohistochemical analyses showed that while both proteins were readily detectable in the liver, ABCG5 was found predominately lining canalicular membranes, whereas ABCG8 was found in association with bile duct epithelia. At the cellular level, ABCG5 appeared to be apically expressed, whereas ABCG8 had a more diffuse expression pattern. Both ABCG5 and ABCG8 appeared to localize apically as shown by co-localization with MRP2. The distribution patterns of ABCG5 and ABCG8 in the gallbladder were very similar to each other. In the small intestine both ABCG5 and ABCG8 appear to line the brush border. However, at the level of the enterocyte, the cellular distribution patterns of ABCG5 and ABCG8 differed, such that ABCG5 was more diffuse, but ABCG8 was principally apical. Using standard deglycosylation methods, ABCG5 and ABCG8 do not appear to be glycosylated, suggesting a difference between human and mouse proteins.We report the distribution patterns of ABCG5 and ABCG8 in human tissues. Cell fractionation studies showed that both proteins co-fractionated in general, but could also be found independent of each other. As predicted, they are expressed apically in both intestine and liver, although their intracellular expression patterns are not completely congruent. These studies support the concept of heterodimerization of ABCG5 and ABCG8, but also support the notion that these proteins may have an independent function.The gastrointestinal tract is the
Coccidioidomycosis of the Testis and Epididymis  [PDF]
Dane E. Klett, Y. Mark Hong
Open Journal of Urology (OJU) , 2013, DOI: 10.4236/oju.2013.32017

This report describes an unusual presentation of a man with a testicular mass who presented in diabetic ketoacidosis to the emergency department. Initial diagnosis was a suspected testicular malignancy based on history, physical exam, and available laboratory and radiological imaging. Due to the possibility of a testicular malignancy, the patient was taken for immediate orchiectomy. Histology revealed coccidioido-mycosis of the left testicle and epididymis. Treatment with the appropriate antifungals was initiated. Despite aggressive multi-disciplinary therapy the patient continued to deteriorate and eventually expired as a result of the massive systemic infection. Literature review revealed the presentation of disseminated genitourinary coccidioidomycosis is rare and seldom reported.

The Design of a Sustainable Competency-Based Human Resources Management : A Holistic Approach
Fanny Klett (IEEE Fellow)
Knowledge Management & E-Learning : an International Journal , 2010,
Abstract: Human capital is a key source of innovation and competitive improvement. Exploiting its potential and boosting its value to the organization involves a systematic process to determine the competencies that are fundamental to achieve enhanced job performance. However, why do organizations often fail to successfully cope with the main challenges of the knowledge and learning society, and to identify the consequences for learning technologies? This paper discusses a holistic model for human resources management strategy required to technologically support the organization toward a forceful and comprehensive solution. By applying a step-wise approach, the components that organizations should consider are identified, along with their interrelationships, and the increased need for a harmonizing world-wide standardization in this application field.
The rat STSL locus: characterization, chromosomal assignment, and genetic variations in sitosterolemic hypertensive rats
Hongwei Yu, Bhaswati Pandit, Eric Klett, Mi-Hye Lee, Kangmo Lu, Khalil Helou, Ikuo Ikeda, Nami Egashira, Masao Sato, Richard Klein, Ashok Batta, Gerald Salen, Shailendra B Patel
BMC Cardiovascular Disorders , 2003, DOI: 10.1186/1471-2261-3-4
Abstract: To investigate whether mutations in Abcg5 or Abcg8 exist in SHR, SHRSP, WKY and GH rats, we initiated a systematic search for the genetic variation in coding and non-coding region of Abcg5 and Abcg8 genes in these strains. We isolated the rat cDNAs for these genes and characterized the genomic structure and tissue expression patterns, using standard molecular biology techniques and FISH for chromosomal assignments.Both rat Abcg5 and Abcg8 genes map to chromosome band 6q12. These genes span ~40 kb and contain 13 exons and 12 introns each, in a pattern identical to that of the STSL loci in mouse and man. Both Abcg5 and Abcg8 were expressed only in liver and intestine. Analyses of DNA from SHR, SHRSP, GH, WKY, Wistar, Wistar King A (WKA) and Brown Norway (BN) rat strains revealed a homozygous G to T substitution at nucleotide 1754, resulting in the coding change Gly583Cys in sterolin-1 only in rats that are both sitosterolemic and hypertensive (SHR, SHRSP and WKY).The rat STSL locus maps to chromosome 6q12. A non-synonymous mutation in Abcg5, Gly583Cys, results in sitosterolemia in rat strains that are also hypertensive (WKY, SHR and SHRSP). Those rat strains that are hypertensive, but not sitosterolemic (e.g. GH rat) do not have mutations in Abcg5 or Abcg8. This mutation allows for expression and apparent apical targeting of Abcg5 protein in the intestine. These rat strains may therefore allow us to study the pathophysiological mechanisms involved in the human disease of sitosterolemia.The human disorder of sitosterolemia, also known as phytosterolemia (MIM 210250), is an autosomal recessive disorder characterized by the marked elevation of plasma phytosterols e.g. β-sitosterol, campesterol and stigmasterol [1,2]. Hypercholesterolemia primarily during childhood, accelerated atherosclerosis in some adult patients leading to premature death, as well a hemolytic anaemia, arthralgias and tendon and tuberous xanthoma formations are other clinical features. Studies have sho
Neuropsychological effects of chronic low-dose exposure to polychlorinated biphenyls (PCBs): A cross-sectional study
Martin Peper, Martin Klett, Rudolf Morgenstern
Environmental Health , 2005, DOI: 10.1186/1476-069x-4-22
Abstract: Thirty employees exposed to indoor air contaminated with PCBs from elastic sealants in a school building were compared to 30 non-exposed controls matched for education and age, controlling for gender (age range 37–61 years). PCB exposure was verified by external exposure data and biological monitoring (PCB 28, 101, 138, 153, 180). Subjective complaints, learning and memory, executive function, and visual-spatial function was assessed by standardized neuropsychological testing. Since exposure status depended on the use of contaminated rooms, an objectively exposed subgroup (N = 16; PCB 28 = 0.20 μg/l; weighted exposure duration 17.9 ± 7 years) was identified and compared with 16 paired controls.Blood analyses indicated a moderate exposure effect size (d) relative to expected background exposure for total PCB (4.45 ± 2.44 μg/l; d = 0.4). A significant exposure effect was found for the low chlorinated PCBs 28 (0.28 ± 0.25 μg/l; d = 1.5) and 101 (0.07 ± 0.09 μg/l; d = 0.7). Although no neuropsychological effects exceeded the adjusted significance level, estimation statistics showed elevated effect sizes for several variables. The objectively exposed subgroup showed a trend towards increased subjective attentional and emotional complaints (tiredness and slowing of practical activities, emotional state) as well as attenuated attentional performance (response shifting and alertness in a cued reaction task).Chronic inhalation of low chlorinated PCBs that involved elevated blood levels was associated with a subtle attenuation of emotional well-being and attentional function. Extended research is needed to replicate the potential long-term low PCB effects in a larger sample.The neurobehavioral effects of polychlorinated biphenyls (PCBs) have been extensively studied in neonates and children [39,62,77]. However, no conclusive evidence is available on chronic nervous system effects in adult humans. The present neuropsychological group study explored the potential cognitive and
Editorial: The War for Talent: Technologies and solutions toward competency and skills development and talent identification
Fanny Klett (IEEE Fellow),Minhong Wang
Knowledge Management & E-Learning : an International Journal , 2013,
Abstract: This special issue is dedicated to advanced technological solutions and novel methodical approaches toward human capital management in terms of career development, assessment and recruitment as a driver for innovation and sustainable competitive advantage for academia and business in the changing conditions of the global employment market, and the War for Talent. Latest competitiveness-driven developments in productivity and services move forward human capital management and assessment technology and services alongside with talent identification as a driver for innovation and key source of maximizing the Return-On-Investment in people and technology in academia and business. Governments and business start thinking about competency and skills development as the critical issue for the workforce, and the workplaces. Against this background, a complex interrelationship arises between strategic management, human capital management, and the overall quality management in every educational and enterprise setting. In addition, identifying highly competent human capital develops into a challenging issue of the recruitment process.
Curadorías jaguares, poéticas de lo múltiple. Una mirada decolonial en la producción de la nueva institucionalidad pública Jaguar Curatorship, the Poetics of the Multiple. A decolonial approach in the production of the new public institutionalism
Azucena Klett,Zoe Mediero,Gerardo Tudurí
Teknokultura : Revista de Cultura Digital y Movimientos Sociales , 2013,
Abstract: Lo colectivo y lo común son categorías que describen y afectan la práctica artística, las nuevas formas de creación y la cultura de la curaduría. Nuestro trabajo se acerca a ellas desde una experiencia compartida, la puesta en marcha de una Fábrica de Cine sin Autor en Intermedi , una institución pública de arte y cultura, situada en Matadero Madrid. Desde un planteamiento autoetnográfico y performativo analizamos las oportunidades que ofrece para la producción de una nueva institucionalidad pública. Una visión situada de la curadoría y la mediación, nos permite discutir el papel que la epistemología política de lo común juega en la redefinición actual de la esfera pública y proponer la afectación decolonial de esta concepción desde la noción de multiplicidad. Público, común, rotación y temporalidad se convierten en las palabras clave que guían este texto. En la construcción del problema vemos cómo el arte y la cultura pueden convertirse en un espacio de ensayo de nuevas formas de gobernanza donde crear, desde modelos poéticos de pensamiento, nuevos futuros para ese espacio público que compartimos. Se abre la pregunta por una curadoría y mediación situada, en el contexto de lo público-institucional, para la composición de una comunidad temporal, variable, en movimiento y transformación, desde un punto de vista decolonial que denominamos jaguar. The collective and the common are categories that describe and affect art practice, the new forms of creation, and the culture of curatorship. This conversation approaches them from a shared experience, the setting in motion of a Factory of Authorless Cinema in Intermedi , a public institution of art and culture, located in Madrid's Matadero. From an auto-ethnographic and performative standpoint, we analyse the opportunities this opens up for the production of a new cultural institution. This situated understanding of curatorship and mediation allows us to discuss the role of the political epistemology of the commons in the current redefinition of the public sphere and to propose a decolonial affecting of this notion from the standpoint of multiplicity. Public, commons, rotation and temporality become the keywords that guide this text. In the construction of the problem we see how art and culture can become a space to play out new forms of governance where, on the basis of poetic models of thought, new futures for this public space we share can be created. The question is posed by a situated curatorship and mediation, in the context of the public institution, for the composition of a temporary, variable community
A mouse model of sitosterolemia: absence of Abcg8/sterolin-2 results in failure to secrete biliary cholesterol
Eric L Klett, Kangmo Lu, Astrid Kosters, Edwin Vink, Mi-Hye Lee, Michael Altenburg, Sarah Shefer, Ashok K Batta, Hongwei Yu, Jianliang Chen, Richard Klein, Norbert Looije, Ronald Oude-Elferink, Albert K Groen, Nobuyo Maeda, Gerald Salen, Shailendra B Patel
BMC Medicine , 2004, DOI: 10.1186/1741-7015-2-5
Abstract: In order to mimic the human disease, we created, by a targeted disruption, a mouse model of sitosterolemia resulting in Abcg8/sterolin-2 deficiency alone. Homozygous knockout mice are viable and exhibit sitosterolemia.Mice deficient in Abcg8 have significantly increased plasma and tissue plant sterol levels (sitosterol and campesterol) consistent with sitosterolemia. Interestingly, Abcg5/sterolin-1 was expressed in both liver and intestine in Abcg8/sterolin-2 deficient mice and continued to show an apical expression. Remarkably, Abcg8 deficient mice had an impaired ability to secrete cholesterol into bile, but still maintained the ability to secrete sitosterol. We also report an intermediate phenotype in the heterozygous Abcg8+/- mice that are not sitosterolemic, but have a decreased level of biliary sterol secretion relative to wild-type mice.These data indicate that Abcg8/sterolin-2 is necessary for biliary sterol secretion and that loss of Abcg8/sterolin-2 has a more profound effect upon biliary cholesterol secretion than sitosterol. Since biliary sitosterol secretion is preserved, although not elevated in the sitosterolemic mice, this observation suggests that mechanisms other than by Abcg8/sterolin-2 may be responsible for its secretion into bile.Absorption of dietary cholesterol from the intestine is an important part of cholesterol homeostasis and represents the initial step that allows dietary cholesterol to exert its metabolic effects. A typical western diet contains relatively equal amounts of cholesterol and non-cholesterol sterols, mainly plant sterols, of which about 55% of the dietary cholesterol is absorbed and retained compared to ~1% of the dietary non-cholesterol sterols [1-3]. Schoenheimer recognized more than 75 years ago that only cholesterol, not non-cholesterol sterols, is absorbed in the intestine, although the exact molecular mechanisms by which preferential cholesterol absorption occurs has not been fully elucidated [4]. Similarly, although
Effect of Pharmaceutical Potential Endocrine Disruptor Compounds on Protein Disulfide Isomerase Reductase Activity Using Di-Eosin-Oxidized-Glutathion
Danièle Klett,Claire Cahoreau,Mélanie Villeret,Yves Combarnous
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0009507
Abstract: Protein Disulfide Isomerase (PDI) in the endoplasmic reticulum of all cells catalyzes the rearrangement of disulfide bridges during folding of membrane and secreted proteins. As PDI is also known to bind various molecules including hormones such as estradiol and thyroxin, we considered the hypothesis that adverse effects of endocrine-disrupter compounds (EDC) could be mediated through their interaction with PDI leading to defects in membrane or secreted proteins.
Explaining gender differences in non-fatal suicidal behaviour among adolescents: a population-based study
Michael Kaess, Peter Parzer, Johann Haffner, Rainer Steen, Jeanette Roos, Martin Klett, Romuald Brunner, Franz Resch
BMC Public Health , 2011, DOI: 10.1186/1471-2458-11-597
Abstract: A cross-sectional design was used to assess suicidal behaviour and various areas of emotional and behavioural problems by using a self-report booklet including the Youth Self-Report. One hundred sixteen schools in a region of Southern Germany agreed to participate. A representative sample of 5,512 ninth-grade students was studied. Mean age was 14.8 years (SD 0.73); 49.8% were female.Serious suicidal thoughts were reported by 19.8% of the female students and 10.8% of the females had ever attempted suicide. In the male group, 9.3% had a history of suicidal thoughts and 4.9% had previously attempted suicide. Internalizing emotional and behavioural problems were shown to be higher in the female group (difference of the group means 4.41) while externalizing emotional and behavioural problems slightly predominated in male students (difference of the group means -0.65). However, the total rate of emotional and behavioural problems was significantly higher in the adolescent female group (difference of the group means 4.98). Using logistic regression models with suicidal thoughts or attempted suicide as dependent variables, the pseudo-R2 of gender alone was only 2.7% or 2.3%, while it was 30% or 23.2% for emotional and behavioural problems measured by the YSR syndrome scales. By adding gender to the emotional and behavioural problems only an additional 0.3% of information could be explained.The findings suggest that gender differences in non-fatal suicidal behaviour among adolescents can to a large extent be explained by the gender differences in emotional and behavioural problems during this age.Suicide and non-fatal suicidal behaviour are both well-recognized public health problems in young people [1,2]. Whereas the prevalence of suicide and suicidal behaviour remains relatively low before puberty [3], adolescent suicide is one of the leading causes of death in the teenaged group [4]. In Europe, suicide is the second leading cause of death in male and female adolescents [5
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