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Search Results: 1 - 10 of 192257 matches for " Elza Fernandes de;Chaves-Alves "
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Partial purification and characterization of xylanase produced by Penicillium expansum
Querido, André Luiz de Souza;Coelho, Jorge Luiz Cavalcante;Araújo, Elza Fernandes de;Chaves-Alves, Virgínia Maria;
Brazilian Archives of Biology and Technology , 2006, DOI: 10.1590/S1516-89132006000400016
Abstract: an extracellular xylanase was found to be the major protein in the filtrate culture of penicillium expansum when grown on 0.3 % wheat bran, which showed no xylanase multiplicity. the enzime was partial purified by.ammonium sulfate fractioning, molecular exclusion chromatography, ultrafiltration and anion exchange chromatography. the protein eluation profile showed only one form of xylanase that was partially characterized. the activity of purified xylanase was optimal at ph 5.5 and 40 oc. the enzyme was stable at ph between 5.5 and 6.5 and temperatures between 20-40 oc. the enzyme showed a km of 3.03 mm and vmax of 0.027 mmol min-1 mg -1 of protein. the enzymatic activity was increased 31 % by mg2+ and 28 % by al3+.
Recursos ecológicos utilizados por las especies de Xylocopa (Apidae: Xylocopini) en el área urbana Ecological resources used by Xylocopa species (Apidae: Xylocopini) in the urban area
TALLES MARQUES CHAVES-ALVES,CAMILA NONATO JUNQUEIRA,LAíCE SOUZA RABELO,PAULO EUGêNIO ALVES MACEDO DE OLIVEIRA
Revista Colombiana de Entomología , 2011,
Abstract: Las áreas verdes de las ciudades, como parques ecológicos, plazas, la forestación de carreteras y incluso jardines domésticos, además de componer el paisaje urbano, también ofrecen recursos para la alimentación y nidificación de muchas especies de abejas, como las del género Xylocopa. En este sentido, el presente estudio tuvo como objetivos identificar los sustratos naturales de nidificación y la disponibilidad de los recursos alimenticios utilizados por Xylocopa spp. en una área urbana, con el fin de verificar el potencial de manejo de estas abejas. Además, se observó el porcentaje de ocupación de estas abejas en substratos utilizados como nidos-trampas. Tres especies de plantas se utilizaron como sustratos, por las abejas Xylocopa, para construir sus nidos: Spathodea campanulata (Bignoniaceae), Ligustrum lucidum (Oleaceae) y Ficus sp. (Moraceae). Los nidos se distribuyeron en agregado. Spathodea campanulata, Ficus sp. y tallos de bambú (nidos-trampas) fueron los sustratos de nidificación más eficientes para Xylocopa spp. y por consiguiente, son adecuados para la conservación y manejo de estas abejas. Las fores de las pocas especies de árboles visitadas por Xylocopa en el área urbana pertenecen a grupos asociados a menudo con las abejas y las fuentes de recursos alimenticios se mostraron diferentes de las especies utilizadas para la nidificación. Las áreas urbanas en condiciones adecuadas, como cuando hay sustratos adecuados para nidificación y recursos alimenticios, pueden servir como sitio de estudio, conservación y manejo de las abejas carpinteras. Green areas of cities such as ecological parks, squares, road forestation and even domestic gardens, besides being part of the urban landscape, also serve as ecological resources for many bee species, as those belonging to Xylocopa genus. In this sense, the present study aimed to identify the natural nesting substrates and food resources available for Xylocopa spp. in order to verify the management potential of these bees in an urban area. Additionally, we observed the percentage of occupancy for these bees on previously available substrates (trap-nests). Three plants were used as nesting substrates: Spathodea campanulata (Bignoniaceae), Ligustrum lucidum (Oleaceae) and Ficus sp. (Moraceae). The nests presented an aggregated distribution. Spathodea campanulata, Ficus sp. and bamboo canes (trap-nests) were considered efficient substrates for Xylocopa spp. nesting and, for this reason, they can be used in management and conservation of these bees. The fowers of the few trees species visited by Xylocopa in u
Recursos ecológicos utilizados por las especies de Xylocopa (Apidae: Xylocopini) en el área urbana
CHAVES-ALVES,TALLES MARQUES; NONATO JUNQUEIRA,CAMILA; SOUZA RABELO,LAíCE; MACEDO DE OLIVEIRA,PAULO EUGêNIO ALVES; AUGUSTO,SOLANGE CRISTINA;
Revista Colombiana de Entomología , 2011,
Abstract: green areas of cities such as ecological parks, squares, road forestation and even domestic gardens, besides being part of the urban landscape, also serve as ecological resources for many bee species, as those belonging to xylocopa genus. in this sense, the present study aimed to identify the natural nesting substrates and food resources available for xylocopa spp. in order to verify the management potential of these bees in an urban area. additionally, we observed the percentage of occupancy for these bees on previously available substrates (trap-nests). three plants were used as nesting substrates: spathodea campanulata (bignoniaceae), ligustrum lucidum (oleaceae) and ficus sp. (moraceae). the nests presented an aggregated distribution. spathodea campanulata, ficus sp. and bamboo canes (trap-nests) were considered efficient substrates for xylocopa spp. nesting and, for this reason, they can be used in management and conservation of these bees. the fowers of the few trees species visited by xylocopa in urban areas belong to groups often associated with bees and the food sources were different from those species used for nesting. the urban environment under appropriate conditions, when appropriate nest substrates and food sources are presented, can serve as a place for study, conservation and management of carpenter bees.
Screening of filamentous fungi for production of xylitol from D-xylose
Sampaio, Fábio Coelho;Silveira, Wendel Batista da;Chaves-Alves, Virgínia Maria;Passos, Flávia Maria Lopes;Coelho, Jorge Luiz Cavalcante;
Brazilian Journal of Microbiology , 2003, DOI: 10.1590/S1517-83822003000400007
Abstract: eleven filamentous fungi were screened for xylitol production in batch cultures. production was generally low under the growth conditions used in this study. penicillium crustosum presented the highest production, 0.52 g l-1 from 11.50 g l-1 of d-xylose, representing consumption of 76% of the original d-xylose.
Thermostability of xylanolytic enzymes produced by Lentinula edodes UFV70
Ribeiro, Liliane Fraga Costa;Vaz, Marcelo Gomes Mar?al Vieira;Chaves-Alves, Virgínia Maria;Vanetti, Maria Cristina Dantas;Kasuya, Maria Catarina Megumi;Passos, Flávia Maria Lopes;Nascimento, Ant?nio Galv?o do;
Brazilian Journal of Microbiology , 2012, DOI: 10.1590/S1517-83822012000100021
Abstract: xylanolytic enzymes produced by lentinula edodes ufv70, cultivated in eucalyptus sawdust/rice bran medium, were stable at 50, 60 and 65oc for 21 hours, losing only 15-25% activity. fungus incubation at 50oc for 12 hours and at 65oc for 24 hours increased the amount of xylose produced.
Crambe (Crambe abyssinica) Cultivation under Different Levels of Fertilization with Nitrogen, Phosphorus and Potassium  [PDF]
Ana Carolina F. de Vasconcelos, Lúcia Helena G. Chaves, Josely Dantas Fernandes, Gilvanise Alves Tito
American Journal of Plant Sciences (AJPS) , 2017, DOI: 10.4236/ajps.2017.89136
Abstract: The effects of fertilization on crambe are poorly understood; in this sense, this study aimed to evaluate the crambe growth under different levels of nitrogen (0, 40, 80, 120 kg·ha-1), phosphorus (0, 50, 100, 150 kg·ha-1), and potassium (0, 30, 60, 90 kg·ha-1) in a greenhouse. The height of the plants, the production of dried biomass and that of 1000 grains of crambe were evaluated. The height of the plants was impaired as a function of increasing doses of nitrogen. The interaction of nitrogen and phosphorus in the crambe crop increased the number of grains and dry shoot biomass. The growth and yield of the crop were not influenced by the use of potassium in mineral fertilization.
Effect of Copper, Zinc, Cadmium and Chromium in the Growth of Crambe  [PDF]
Gilvanise Alves Tito, Lúcia Helena Garófalo Chaves, Josely Dantas Fernandes, Danilo Rodrigues Monteiro, Ana Carolina Feitosa de Vasconcelos
Agricultural Sciences (AS) , 2014, DOI: 10.4236/as.2014.511105
Abstract: Crambe is a promising crop for biodiesel production. However, there is no much information available about techniques and research regarding the best performance in different regions to explore its potential. The objective of this study was to evaluate the effect of cadmium, chromium, copper and zinc in the development of crambe. The study was carried out in a greenhouse and it consisted of four independent experiments in completely randomized design, with three replications. Four metals with five levels were applied: 0, 10, 15, 20 and 25 mg·kg-1 for copper, cadmium, chromium; and 0, 20, 30, 40 and 50 mg·kg-1 for zinc, in order to evaluate the growth of crambe. Data were subjected to analysis of variance and comparison of means by Tukey test at 1 and 5% significance. Despite doses of cadmium, chromium, copper and zinc in growing crambe have influenced in some of the evaluated variables of the plants, in general, their development was similar in relation to doses and the metals applied. The importance of cultivating crambe is related to grain production, based on this, copper and cadmium decreased this production.
Mapping of QTLs related with wood quality and developmental characteristics in hybrids (Eucalyptus grandis x Eucalyptus urophylla)
Rocha, Rodrigo Barros;Barros, Everaldo Gon?alves;Cruz, Cosme Dami?o;Rosado, Ant?nio Marcos;Araújo, Elza Fernandes de;
Revista árvore , 2007, DOI: 10.1590/S0100-67622007000100003
Abstract: the present work aimed to characterize and identify qtls for wood quality and growth traits in e. grandis x e. urophylla hybrids. for this purpose a rapd linkage map was developed for the hybrids (lod=3 and r=0.40) containing 52 markers and 12 linkage groups. traits related to wood quality and growth were evaluated in the qtl analyses. qtl analyses were performed using chi-square tests, single-marker, interval mapping and composite interval mapping analyses. all approaches led to the identification of similar qtls associated with wood density, cellulose pulp yield and percentage of extractives, which were detected and confirmed by both the interval mapping and composite interval mapping methodologies. some qtls regions were confirmed only by the composite interval mapping methodology: percentage of soluble lignin, percentage of insoluble lignin, cbh and total height. overlapping qtls regions were detected, and these, can be the result of major genes involved in the regulation and control of the growth traits by epistatic interactions. in order to evaluate the effect of early selection using rapd molecular data, molecular markers adjacent to qtls were used genotype selection. the analysis of selection differential values suggests that for all the traits the phenotypic selection at seven years should generate larger genetic gains than early selection assisted by molecular markers and the combination of the strategies should elevate the selection efficiency.
Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum
Varavallo, Maurilio Antonio;Queiroz, Marisa Vieira de;Lana, Taís Guimar?es;Brito, Admilson Toscano Ribeiro de;Gon?alves, Daniel Bonoto;Araújo, Elza Fernandes de;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000100011
Abstract: protoplast fusion between complementary auxotrophic and morphological mutant strains of penicillium griseoroseum and p. expansum was induced by polyethylene glycol and calcium ions (ca2+). fusant strains were obtained in minimal medium and a prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. different recombinant strains were isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. the fusant prototrophic did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented enhanced enzyme activities. the recombinant rge27, which possesses the same auxotrophic and morphologic mutations as the p. griseoroseum parental strain, presented a considerable increase in polygalacturonase (3-fold) and pectin lyase production (1.2-fold).
Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas Gerais, Brazil
Torres, Adalgisa Ribeiro;Cursino, Luciana;Muro-Abad, Júpiter Israel;Gomes, Eliane Aparecida;Araújo, Elza Fernandes de;Hungria, Mariangela;Cassini, Sérvio Túlio Alves;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000400016
Abstract: we characterized indigenous common bean rhizobia from five districts of the state of minas gerais, brazil. the isolates were trapped by two common bean varieties, the mineiro precoce (andean origin) and ouro negro (mesoamerican origin). analysis by box-pcr of selected isolates detected a high level of genetic diversity.
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