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Search Results: 1 - 10 of 30736 matches for " Elmiro Rosendo do;Pereira "
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In vitro behavior of Mycoplasmagallisepticum live-type nosode
M???′sar Lemos,Elmiro Rosendo do Nascimento,Maria Lucia Barreto,Virginia Leo de Almeida Pereira
International Journal of High Dilution Research , 2011,
Abstract: As a step of a doctoral research project, in this study a live-type nosode was prepared from microorganism Mycoplasmagallisepticum strain R (ATCC 93-08/19610) according to Costa model and the rules by Brazilian Homeopathic Pharmacopoeia. Live nosode was tested in vitro to assess safety when used to immunize domestic fowl (Gallus gallus) against infection by this microorganism and to investigate its behavior under laboratory conditions. M. gallisepticum was not shown to grow in fluid (broth) and solid (plate) modified Frey medium with dilutions 11d, 12d, 20d and 30d. Inhibition halos about 2.0 mm were observed around paper disks impregnated with live-type nosode in microorganism-sown Petri dishes, whereas disks impregnated with conventional antibiotic oxytetracycline exhibited 8.0 mm inhibition halos. Protein assessment by Folin-Lowry method showed protein absence in dilutions 12d and 30d and neither microbial DNA traces were found in PCR assay in dilutions 12d, 20d and 30d.
Mycoplasma synoviae infection on Newcastle disease vaccination of chickens
Silva, Rita de Cássia Figueira;Nascimento, Elmiro Rosendo do;Pereira, Virgínia Léo de Almeida;Barreto, Maria Lúcia;Nascimento, Maria da Gra?a Fichel do;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000200033
Abstract: newcastle disease is characterized by respiratory manifestations in association with nervous and/or digestive symptoms. its prevention is done by vaccination with live attenuated (lentogenic strains) and/or killed vaccines. the lentogenic strains can lead to strong post-vaccination reaction, principally due to the presence of other pathogenic agents. among them, mycoplasma synoviae is worldwide important, mainly in brazil. the dissemination of this agent in poultry flocks has been achieved due to difficulties in diagnosis and disease reproduction, virulence variations among different m.synoviae strains, and attribution of typical m.synoviae disease manifestation to other disease agents. this experimental study in spf chicks (gallus gallus), previously infected by m.synoviae and thereafter vaccinated against newcastle disease, was done with the objective of evaluating m.synoviae pathogenicity through assessment of post-vaccinal respiratory reactions and serologic responses to newcastle disease virus vaccine in the absence of environmental factors. a total of 86 three days old chicks were used, being 57 infected by eye and nostril drop, with chicken activated m. synoviae strain wvu 1853. seven days later, 21 mycoplasma infected birds plus 29 not mycoplasma infected ones were vaccinated against newcastle disease. as results, the not infected and vaccinated birds yielded, significantly, higher and longer lasting serologic responses to newcastle disease vaccine virus than those infected and vaccinated. similarly, the infected and vaccinated birds yielded lower serologic reactions to m.synoviae than those only mycoplasma infected. no post-vaccinal respiratory reaction was observed in the vaccinated birds.
Potentially pathogenic mycoplasmas in the external ear canal of clinically normal cattle in Southeast Brazil: first report
Santos, Sandra Batista dos;Nascimento, Elmiro Rosendo do;Faccini, Jo?o Luiz Horácio;Barreto, Maria Lúcia;Pereira, Virginia Léo de Almeida;
Brazilian Journal of Microbiology , 2009, DOI: 10.1590/S1517-83822009000300006
Abstract: mycoplasmas were searched in the ear canal flushing of 60 bovine in brazil. the prevalence obtained was 80%. the percentages of typified species were 12.5%, for m. alkalenses; 2.1%, m. arginini; 8.35%, m. bovirhinis; 2.1%, m. bovis; 25.0%, m. conjunctivae; 14.6%, m. mycoides subsp. mycoides lc and 10.4% m. capricolum.
Perfil de sensibilidade antimicrobiana e detec??o do gene ISS pela rea??o em cadeia da polimerase na tipifica??o de Escherichia coli patogênica em codornas de corte sob inspe??o sanitária
Abreu, Dayse Lima da Costa;Franco, Robson Maia;Nascimento, Elmiro Rosendo do;Pereira, Virginia Léo de Almeida;Alves, Fernanda Martinez Xavier;Almeida, Juliana Ferreira de;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000500006
Abstract: the pathogenicity of escherichia coli strains is partially related to the expression of virulence factors genes, present in genetic elements called plasmids. apec strains responsible for diseases in birds may present the iss gene which increases the resistance of e. coli strains to the lityc effect of the host's serum, besides resistance to several antimicrobials. this study was conduced in order to detect e. coli in tracheae of meat-type quails and to evaluate, by the presence of the iss gene and the profile of antimicrobial susceptibility, the pathogenic potential of the isolated samples for birds and humans. one hundred and eighty tracheae of quails were collected for detection of e. coli, antimicrobial sensitivity tests, and for polymerase chain reaction (pcr), for detection of iss gene. from the examined quails, 8.9 % (16/180) were positive for e. coli, from which 20 strains of this bacterium were obtained. most of them were resistant to tetracycline (16/20), followed by ceftadizime (13/20) and nalidixic-acid (12/20) and only one isolate was resistant to amoxicillin. the detection of iss gene occurred in 55% (11/20) of the isolates, indicating that these strains had the potential to be pathogenic not only for quails, but also for other kinds of birds, other animals and even human beings that would be in contact with these e. coli isolates.
Investiga??o de Campylobacter fetus e Tritrichomonas foetus na mucosa prepucial de touros da regi?o do Médio Paraíba, RJ
Rocha, Flávio Soares da;Jesus, Vera Lúcia Teixeira de;Torres, Helenita Marques;Gomes, Marcos José Pereira;Figueiredo, Márcio José de;Nascimento, Elmiro Rosendo do;Ferreira, Teresinha;Aquino, Maria Helena Cosendey de;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782009005000103
Abstract: thirty nine breeding bulls from dairy farms (n=9) and beef farms (n=30) located in médio paraíba region at rio de janeiro - brazil state were investigated for the presence of campylobacter fetus and tritrichomonas foetus. for campylobacter investigation, smegma samples were examined by culture and prepucial washings were examined by direct immunofluorescence technique (dif). the prepucial washings were also examined for tritrichomonas foetus presence by direct examination. c. fetus was identified in 14 samples (35.9 %) by dif technique and c. fetus subspecies venerealis was isolated from four samples (10.3%). t. foetus was not detected in bull samples. the high frequency of c. fetus observed in bull samples suggests the occurrence of campylobacteriosis among herds which have reproductive problems at the médio paraíba region.
Detec??o do Grupo Mycoplasma mycoides por imunoperoxidase indireta (IPI) e PCR-REA em conduto auditivo de bovinos
Santos, Sandra Batista dos;Nascimento, Elmiro Rosendo do;Faccini, Jo?o Luiz Horacio;Barreto, Maria Lúcia;Almeida, Juliana Ferreira de;Pereira, Virginia Léo de Almeida;Campos, Carlos Augusto Martino;
Pesquisa Veterinária Brasileira , 2010, DOI: 10.1590/S0100-736X2010000500015
Abstract: mycoplasma mycoides cluster (mmc) was diagnosed by polimerase chain reaction-restriction endonuclease analysis (pcr-rea) and indirect immunoperoxidase (ipi), both, carried out in flushing from external ear canal, collected from bovine at slaughter time in the state of rio de janeiro, southeastern, brazil. a total of 60 bovines were randomly selected. sterile syringes (60ml) loaded with buffer solution (pbs, ph 7.2) were used for the ear canal flushing. the obtained samples were stored in glycerol (1:2) and frozen at -20oc until use. these specimens were diluted up to 10-5, inoculated in liquid and solid modified hayflick′s media and incubated at 37oc for 2-3 days. the plates were kept in a microaerophilia condition and examined every two days under a stereomicroscope for the presence of typical colonies “fried-egg”. in this study, 35 strains selected in agreement with their biochemistry and physiologic proprieties, were used. from the 60 cultivated samples, 48 (80.00%) were positive for mycoplasma spp. under ipi the prevalence obtained for mmc was 20.0% (12/60) while by pcr-rea it was 41.7% (25/60). the ipi typing of these isolates resulted in 58.3% (7/12) for m.mycoides mycoides lc and 41.7% (5/12) for m. capricolum. pcr-rea for mmc was confirmed by the amplicon size of 785bp, compatible with this group. the kappa value for the association between these two tests was 0.14 (p>0.05). after restriction analysis with alui in all mmc strains the fragments size obtained were of 81, 98, 186 and 236bp, but not of 370bp that is compatible with mycoides mycoides mycoides sc of bovine type. the presence of mycoplasmas species in the ear canal of asymptomatic bovines represent a risk of subsequent propagation of mycoplasma spp. among bovine herds in brazil.
Rapid diagnosis of vesicular stomatitis virus in Ecuador by the use of polymerase chain reaction
Sepúlveda, Lya Madureira;Malirat, Viviana;Bergmann, Ingrid E.;Mantilla, Anibal;Nascimento, Elmiro Rosendo do;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000300022
Abstract: vesicular stomatitis (vs) is a viral disease that has a great impact in animal health, as infected animals present marked decrease in meat and milk production. its presence is a limiting factor for international animal trade. besides the damage in the livestock productivity, such disease assumes an important role in animal health programs since it is clinically indistinguishable from foot-and-mouth disease. the diagnosis of the vs has been made, mainly, through complement fixation, elisa and virus neutralization tests, assays that allow not only for viral detection but also for differentiation of the two serotypes described for vesicular stomatitis virus (vsv): new jersey (nj) and indiana (ind). in this work, a molecular diagnostic approach, the polymerase chain reaction performed after reverse transcription (rt - pcr), based on the specific partial amplification of ns gene of vsv was used, as an alternative method for the detection of the virus. a total of 10 vsv reference samples and 12 specimens collected from animals with clinical signs of vesicular disease obtained from field episodes in ecuador were tested. the method allowed for the specific partial amplification of the region coding for protein p, both for vsv serotypes new jersey (642 bp) and indiana 1 (614 bp). the results were compatible with data obtained by complement fixation test and the identity of the amplified products was confirmed by nucleotide sequencing.
Etiologia das infec es intramamárias em vacas primíparas no período pós-parto
Pardo Paulo Eduardo,Mettifogo Elena,Müller Ernst Eckehardt,Nascimento Elmiro Rosendo do
Pesquisa Veterinária Brasileira , 1998,
Abstract: Foram analisadas 664 amostras de leite de 83 vacas primíparas da ra a Holandesa. As amostras foram colhidas no primeiro e no sétimo dia após o parto. Das 664 amostras analisadas, 488 (73,50%) foram bacteriologicamente negativas e 176 (26,50%), positivas para microrganismos aeróbios. Foi observado um alto índice de mastite clínica (20,48%). Os agentes isolados com maior freqüência foram os Staphylococcus spp coagulase negativo (64,20%), Staphylococcus spp coagulase positivo (8,52%), Streptococcus spp (7,96%), Actinomyces pyogenes (4,55%), Mycoplasma bovigenitalium (3,40%) e Escherichia coli (2,84%). Foi observado um maior índice de isolamento de patógenos no primeiro dia (17,62%) em rela o ao sétimo (8,88%).
Detection of Mycoplasma pulmonis in laboratory rats
Barreto Maria Lucia,Nascimento Elmiro Rosendo do,Campos Carlos Augusto de Martino,Nascimento Maria da Gra?a Fichel do
Brazilian Journal of Microbiology , 2002,
Abstract: This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25% (13/16) from the conventional controlled premises, and 25.00% (3/12) from the conventional premises. The ELISA test was carried out in 20 animals of both colonies. In the conventional controlled premises, 92.86% (13/14) were positive for M. pulmonis, and 7.14% (1/14) were suspicious, while in the conventional premises, 100% (6/6) of the samples were positive. The results confirmed that M. pulmonis was the etiologic agent of the disease that affected the rats under study, and that the ELISA positivity rated higher than culture.
Juliana Ferreira de Almeida,Carlos Henrique da Silva Leit?o,Elmiro Rosendo do Nascimento,Karine de Castro Meireles Vieira
Ciência Animal Brasileira , 2009,
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