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Search Results: 1 - 10 of 32410 matches for " Elisa San Martin Mouriz;Presotto "
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First occurrence of an autochthonous canine case of Leishmania (Leishmania) infantum chagasi in the municipality of Campinas, State of S?o Paulo, Brazil
Savani, Elisa San Martin Mouriz;Presotto, Douglas;Roberto, Thais;Camargo, Maria Cecília Gibrail de Oliveira;D'auria, Sandra Regina Nicoletti;Sacramento, Débora Veiga;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2011, DOI: 10.1590/S0036-46652011000400010
Abstract: an autochthonous case of visceral leishmaniasis is reported in a dog (canis familiaris) as an apparently natural infection in a non-endemic area. dna obtained from spleen and liver samples produced the expected fragment in a leishmania-specific rdna-based nested-pcr assay. the pcr product, a 490 bp fragment, was sequenced and the nucleotide sequence was identical to that of leishmania (leishmania) infantum chagasi. these results are surprising since no autochthonous human or canine cases of visceral leishmaniasis have ever been reported in this municipality. this case suggests that natural transmission of this disease is occurring in this area.
Vigilancia de leishmaniose visceral americana em c?es de área n?o endêmica, S?o Paulo
Savani,Elisa San Martin Mouriz; Schimonsky,Bernhard von; Camargo,Maria Cecilia Gibrail Oliveira; D'auria,Sandra Regina Nicoletti;
Revista de Saúde Pública , 2003, DOI: 10.1590/S0034-89102003000200016
Abstract: a serological survey among domestic and stray dogs of the municipality of s?o josé do rio preto, brazil, was conducted to identify infected animals and detect the chances of transmitting the american visceral leishmaniasis . from november 1998 to june 2000, 2,104 sera samples were tested by indirect immunofluorescence reaction, using as antigen promastigotes of leishmania (l.) chagasi. there were 2,092 no reactive samples and 12 reactive ones. of the dogs with reactive serum it was possible to prepare lesion smears in only 3. the slide was stained by giemsa method and only in one of them it was found amastigotes forms characteristic of leishmania sp. this finding suggests the need of canine serological and entomological surveillance in s?o josé do rio preto for an early detection of any local epidemiological changes.
Ocorrência de Leishmania spp. em felinos do município de Ara atuba, SP Occurrence de Leishmania spp. in domestic cats from Ara atuba, SP
Katia Denise Saraiva Bresciani,Ana Claudia Marques Serrano,Lucas Vinicius Shigaki de Matos,Elisa San Martin Mouriz Savani
Revista Brasileira de Parasitologia Veterinária , 2010, DOI: 10.1590/s1984-29612010000200012
Abstract: Este trabalho teve como objetivo comparar a ocorrência de Leishmania spp. em gatos por dois métodos (citológico e sorológico), bem como associar a ocorrência deste protozoário com as variáveis sexo, idade e ra a. Amostras séricas de 283 felinos domésticos foram testadas pela Rea o de Imunofluorescência Indireta (RIFI), e o exame parasitológico direto de linfonodos também foi realizado para a verifica o da positividade para Leishmania spp. Ocorrência de 0,7% (2/283) foi observada nos felinos examinados, por meio de imprint de linfonodos e nenhum animal apresentou títulos de anticorpos para Leishmania spp. As duas fêmeas positivas eram sem ra a definida, sendo uma jovem e outra adulta. Por meio dos resultados obtidos, n o foi constatada diferen a estatisticamente significante em rela o às variáveis sexo, ra a e idade nos gatos desta pesquisa (p > 0,05). Ocorrência de Leishmania spp. nos gatos deste estudo foi baixa. Devido a esta baixa incidência sugere-se que estes n o assumem importancia epidemiológica na área do estudo. This study had the purpose to compare the occurrence of Leishmania spp. in felines through two methods (cytological and serological), as well as to associate the occurrence of this protozoan with the sex, age and breed variables. Serum samples from 283 domestic felines were processed by means of Indirect Immunofluorescence Reaction (IIR), and the direct parasitological test for linfonodes was also carried out in order to verify positivity for Leishmania spp. Occurrence of 0.7% (2/283) was observed in the tested felines by means of linfonode imprinting and no animal showed title of antibodies for Leishmania spp. The two positive females were mongrel, a young female and an adult female feline. From the obtained results, no statistically significant difference was observed as regards the sex, breed and age variables in this research (p > 0.05). Occurrence of Leishmania spp. in the cats of this study was low. Such low incidence suggests that these hosts has no epidemiological relevance in the study area.
Vigilancia de leishmaniose visceral americana em c es de área n o endêmica, S o Paulo
Savani Elisa San Martin Mouriz,Schimonsky Bernhard von,Camargo Maria Cecilia Gibrail Oliveira,D'auria Sandra Regina Nicoletti
Revista de Saúde Pública , 2003,
Abstract: Realizou-se inquérito sorológico em c es domiciliados e errantes do Município de S o José do Rio Preto, SP, para identificar animais infectados e detectar a possibilidade de transmiss o da leishmaniose visceral americana. De novembro de 1998 a junho de 2000, 2.104 amostras de soros foram testadas por meio da rea o de imunofluorescência indireta, empregando-se como antígeno formas promastigotas de Leishmania (L.) chagasi. Observaram-se 2.092 amostras n o reagentes e 12 reagentes. Dos c es com sorologia positiva foi possível realizar raspados de les o em três animais. O material fixado em laminas foi corado por Giemsa e, em apenas um, foram encontradas formas amastigotas características de Leishmania sp. Este resultado indica a necessidade de manuten o da vigilancia sorológica canina e entomológica no município de S o José do Rio Preto, a fim de detectar, precocemente, qualquer altera o na epidemiologia local.
Serological identification of Rickettsia spp from the spotted fever group in capybaras in the region of Campinas - SP - Brazil
Souza, Celso Eduardo de;Souza, Savina Silvana Lacerra de;Lima, Virgília Luna Castor;Calic, Simone Berger;Camargo, Maria Cecilia Gibrail Oliveira;Savani, Elisa San Martin Mouriz;D'Auria, Sandra Regina Nicoletti;Linhares, Arício Xavier;Yoshinari, Natalino Hajime;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000600031
Abstract: diseases transmitted by ticks have been an important health problem all over the world. brazilian spotted fever (bsf) stands for a serious epidemiological concern due to the high mortality rates pointed out. capybaras are commonly incriminated as possible reservoirs in the bsf transmission cycle. in the last decades the numbers of these animals raised sharply and they have invaded human areas. they intensify the contact between ticks and humans beings. this study aim is to contribute to the possible role performed for this rodent in the bsf epidemiology in some areas located in campinas region, s?o paulo. cabybaras infected by rickettsiae of bsf group were studied through the analysis of the frequencies of bsf-group rickettisae antibodies titer = 64 by indirect immunofluorescence test (ifa), and data from human cases epidemiological surveillance. the serum frequency positiveness varied greatly according to areas where animals were captured. however it was found serum positiviness only in the areas where human cases of bsf were reported. these findings suggest the capybara may be seen as sentinel animal. due to presence of serological cross reactivity between microorganisms belonging to sf group, the results must be interpreted carefully and additional methods to distinguish pathogenic rickettsiae are required in our country.
Ocurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the state of Mato Grosso do Sul, Brazil
Savani, Elisa San Martin Mouriz;Nunes, Vania Lúcia Brand?o;Galati, Eunice Aparecida Bianchi;Castilho, Tiago Moreno;Araujo, Fernando Shiroma de;Ilha, Iêda Maria Novaes;Camargo, Maria Cecília Gibrail de Oliveira;D'Auria, Sandra Regina Nicoletti;Floeter-Winter, Lucile Maria;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000700011
Abstract: a natural case of co-infection by leishmania and trypanosoma is reported in a dog (canis familiaris) in south- western state of mato grosso do sul, brazil. both amastigote and trypomastigote forms were observed after giemsa staining of cytological preparations of the dog's bone marrow aspirate. no parasite was detected using medium culture inoculation of the sample. dna obtained from the bone marrow aspirate sample and from the blood buffy coat was submitted to polymerase chain reaction (pcr) with a set of rdna-based primers s4/s12. the nucleotide sequence of the pcr product was identical to that of trypanosoma (trypanozoon) evansi. the s4/s12 pcr was then used as template in a nested-pcr using a specific leishmania set s17/s18 as primers, to explain the amastigote forms. the nucleotide sequence of the new pcr product was identical to that of leishmania (leishmania) chagasi. this case, as far as we know, is the first report of a dog co-infected with these parasites, suggesting that besides l. (l.) chagasi, the natural transmission of t. (t.) evansi occurs in the area under study.
Ocorrência de Leishmania spp. em felinos do município de Ara?atuba, SP
Bresciani, Katia Denise Saraiva;Serrano, Ana Claudia Marques;Matos, Lucas Vinicius Shigaki de;Savani, Elisa San Martin Mouriz;D'Auria, Sandra Regina Nicoletti;Perri, Silvia Helena Venturoli;Bonello, Fabio Luiz;Coelho, Willian Marinho Dourado;Aoki, Carolina Godoi;Costa, Alvimar José da;
Revista Brasileira de Parasitologia Veterinária , 2010, DOI: 10.4322/rbpv.01902012
Abstract: this study had the purpose to compare the occurrence of leishmania spp. in felines through two methods (cytological and serological), as well as to associate the occurrence of this protozoan with the sex, age and breed variables. serum samples from 283 domestic felines were processed by means of indirect immunofluorescence reaction (iir), and the direct parasitological test for linfonodes was also carried out in order to verify positivity for leishmania spp. occurrence of 0.7% (2/283) was observed in the tested felines by means of linfonode imprinting and no animal showed title of antibodies for leishmania spp. the two positive females were mongrel, a young female and an adult female feline. from the obtained results, no statistically significant difference was observed as regards the sex, breed and age variables in this research (p > 0.05). occurrence of leishmania spp. in the cats of this study was low. such low incidence suggests that these hosts has no epidemiological relevance in the study area.
Ocorrência de leishmaniose visceral canina em assentamento agrícola no Estado de Mato Grosso do Sul, Brasil
Nunes, Vania Lúcia Brand?o;Galati, Eunice Aparecida Bianchi;Nunes, Daniela Brand?o;Zinezzi, Rodrigo de Oliveira;Savani, Elisa San Martin Mouriz;Ishikawa, Edna;Camargo, Maria Cecília Gibrail de Oliveira;D'áuria, Sandra Regina Nicoletti;Cristaldo, Geucira;Rocha, Hilda Carlos da;
Revista da Sociedade Brasileira de Medicina Tropical , 2001, DOI: 10.1590/S0037-86822001000300014
Abstract: during previous research on phlebotomine fauna in a settlement of the brazilian national agrarian reform institute (incra) on the bodoquena range, mato grosso do sul state, brazil, dogs were observed with clinical aspects suggestive of visceral leishmaniasis. a serological survey to leishmaniasis in 97 dogs, by indirect immunofluorescence test, showed 23 (23.7%) serum positive dogs. samples of the parasites were identified as leishmania (leishmania) chagasi.
Inquérito sorológico sobre leishmaniose tegumentar americana em c es errantes no Estado de S o Paulo, Brasil
Savani Elisa San Martin M,Galati Eunice AB,Camargo Maria Cecília GO,D'Auria Sandra Regina N
Revista de Saúde Pública , 1999,
Abstract: Inquérito sorológico para leishmaniose tegumentar americana em 973 c es errantes, capturados próximos a áreas verdes do Município de S o Paulo, por meio da rea o de imunofluorescência indireta, n o demonstrou soros reagentes. No entanto, casos humanos autóctones dessa zoonose ocorridos no município indicam a circula o do parasita no ambiente. A existência de vetores e a aparente ausência de c es infectados nas áreas pesquisadas sugerem que o c o errante desempenhe papel irrelevante na dissemina o do protozoário.
Inquérito sorológico sobre leishmaniose tegumentar americana em c?es errantes no Estado de S?o Paulo, Brasil
Savani,Elisa San Martin M; Galati,Eunice AB; Camargo,Maria Cecília GO; D'Auria,Sandra Regina N; Damaceno,José T; Balduino,Salete A;
Revista de Saúde Pública , 1999, DOI: 10.1590/S0034-89101999000600017
Abstract: a serological survey was made in 973 stray dogs caught near green areas in s. paulo county by the indirect immunofluorescence test. no positive serum was found, however autochthonous human cases of acl that occurred in the county show the circulation of the parasite in the environment. this fact, associated with the existence of the vectors and the apparent absence of infected dogs in the areas studied, suggest that stray dog plays an insignificant role in the spread of the parasite. the cycle of the parasite in s?o paulo county has been maintained by wild animals, hence the dog would be an accidental host just as humans.
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