oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Search Results: 1 - 10 of 2477 matches for " Eliana Reiko; "
All listed articles are free for downloading (OA Articles)
Page 1 /2477
Display every page Item
Histopathological, immunohistochemical and ultraestructural evaluation of inflammatory response in Arius genus fish under experimental inoculation of BCG
Sado, Ricardo Yuji;Matushima, Eliana Reiko;
Brazilian Archives of Biology and Technology , 2008, DOI: 10.1590/S1516-89132008000500009
Abstract: the aim of this study was to evaluate the inflammatory response kinetics after experimental inoculation with bcg in the primitive arius sp. fish. the bcg was applied through the intramuscular injection in the caudal peduncular region, and the samples were collected for the analyses at days 1, 3, 7, 14, 21, and 33 post-injection. acute phase inflammatory infiltrate was characterized by the predominant mononuclear cells, intersticial edema, and muscular tissue necrosis. as the inflammatory response evolved, a large number of multinuclear giant cells were formed containing the bcg. these giant cells were positive for the s100 protein at the histochemical analysis, which demonstrate the macrofage activity, confirmed by the ultra-structural analysis showing the lack of the cytoplasmic membrane enveloping the many nuclei within the giant cell. these results led to the conclusion that arius sp. fish injected with the bcg showed a difuse inflammatory response characterized by a large number of mononuclear cells, absence of granuloma formation, and predominant giant cells.
Interaction of general adaptation syndrome and myocarditis causing ventricular rupture in a black swan (Cygnus atratus): case report
REGO, Alexandre Aparecido Mattos da Silva;MATUSHIMA, Eliana Reiko;FEREIRA, Antonio José Piantino;PINTO, Celso Martins;
Brazilian Journal of Veterinary Research and Animal Science , 1998, DOI: 10.1590/S1413-95961998000500006
Abstract: case report about the interaction of chronic myocarditis and general adaptation syndrome ("stress") causing rupture of left ventricular wall on a young, female specie of cygnus atratus (black swan), that came to die at the country zoo of tabo?o da serra, in s?o paulo, during quarantine, after being taken on a long distance travel, under disfavorable temperature and ventilation conditions.
Aspectos morfológicos e citoquímicos dos glóbulos sangüíneos de Caiman crocodilus yacare (Daudin, 1802) (Reptilia, Crocodilia)
MOURA, Weber Leal de;MATUSHIMA, Eliana Reiko;OLIVEIRA, Luiz Waldemar;EGAMI, Mizue Imoto;
Brazilian Journal of Veterinary Research and Animal Science , 1999, DOI: 10.1590/S1413-95961999000100009
Abstract: samples of peripheral blood were collected from 10 healthy caiman crocodilus yacare and stained with leishman, for morphological analysis, or submitted to the following cytochemical methods: pas, sudan black b, o-toluidine and mercury bromophenol blue. seven types of cells were identified: erythrocytes, thrombocytes, heterophils, eosinophils, basophils, lymphocytes and azurofilic monocytes. erythrocytes were elliptical and positive for bromophenol blue. some mitotic figures were noted. spindle-shaped thrombocytes showed large nucleus with striking indentation. the cytoplasm was attenuated at one or both poles into knob-like projections containing glycogen. heterophils revealed round eccentric nuclei and cytoplasm containing glycogen and many polymorphic granules. these granules showed partial positive reaction for sudan and activity for myeloperoxidase. eosinophils showed eccentrically located lenticular-shaped nuclei and numerous round granules stained positive with myeloperoxidase and sudan. basophils revealed a round central nuclei and cytoplasm with few basophilic round granules. lymphocytes appeared as polygonal or irregular cells with thin cytoplasm forming bleb-like protrusions. azurophilic monocytes were round with eccentrically located nuclei and basophilic cytoplasm containing numerous azurophilic granules.
Chondroma in rufescent tiger-heron (Tigrisoma lineatum): case report
REGO, Alexandre Aparecido Mattos da Silva;MATUSHIMA, Eliana Reiko;MIGUEL, Maria Candida;
Brazilian Journal of Veterinary Research and Animal Science , 1999, DOI: 10.1590/S1413-95961999000100008
Abstract: the paper describes a chondroma affecting the left metacarpus of a free-ranging rufescent tiger-heron (tigrisoma lineatum). there are very few reports of such neoplastic process in this avian species.
Cytochemical aspects of the peripheral blood cells of Oreochromis (Tilapia) niloticus. (Linnaeus, 1758) (Cichlidae, Teleostei): part II
UEDA, Ivete Kotomi;EGAMI, Mizue Imoto;SASSO, Wilson da Silva;MATUSHIMA, Eliana Reiko;
Brazilian Journal of Veterinary Research and Animal Science , 2001, DOI: 10.1590/S1413-95962001000600005
Abstract: morphologicaly, seven types of cells were identified in the blood of oreochromis niloticus: erythrocytes, thrombocytes, neutrophils, eosinophils, basophils, lymphocytes and monocytes. glycogen was present not only in the cytoplasm of neutrophils and thrombocytes but also in some lymphocytes and monocytes. the positive reaction for myeloperoxidase and sudan black was observed in neutrophils and eosinophils. the bromphenol blue method was strongly positive for erythrocytes and eosinophils.
Principais processos neoplásicos encontrados em psitacídeos mantidos em cativeiro
Godoy, Silvia Neri;Alves, Venancio A. Ferreira;Kanamura, Cristina Takami;Matushima, Eliana Reiko;
Pesquisa Veterinária Brasileira , 2009, DOI: 10.1590/S0100-736X2009000600001
Abstract: an anatomopathologic study of 130 captivy psittacines from zoos, breeders and rehabilitation centers showed the presence of neoplastic disease in seven birds, totalizing 5.4% of the cases. the neoplasms were identified like cholangiocarcinoma, cholangioma, lymphoma, haemangiosarcoma and renal cell carcinoma. the cholangiocarcinoma was the tumor most commonly observed. it was present in birds. this study describes the macro and microscopic findings of these tumors; also reports diagnostic methods employed to identify these neoplasms.
CARACTERISTICAS CITOQUIMICAS MORFOLOGICAS Y ULTRAESTRUCTURALES DE EOSINOFILOS DE Caiman crocodilus yacare (Daudin, 1802) (Reptilia, Crocodilia)
Waldemar Oliveira,Luiz; Leal de Moura,Weber; Reiko Matushima,Eliana; Imoto Egami,Mizue;
Revista chilena de anatomía , 1998, DOI: 10.4067/S0716-98681998000200012
Abstract: summary: samples of peripheral blood from healthy young reptiles were collected from the cervical venous sinus in tubes containing edta 10%. freshly prepared smears were used for the following purpuses: a) staining in rosenfeld solution for the morphological analyses. b) detection of alkaline phosphatase with naphthol asmx-phosphate and myeloperoxidase (ph 7,0 and ph10,0) with o-toluidine h202. the same blood samples with edta were centrifuged for 15' at 2,000rpm and the buffy coat containing leukocytes was devided in two fragments and one of them treated with glutaraldehyde (1h at 4c) and used for alkaline phosphatase reaction and the other 1,25% glutaraldehyde + 1% paraformaldehyde (1h 4c) and used for myeloperoxidase reaction. the eosinophils revealed round, oval or lobed eccentric nuclei with prominent nucleoli. the cytoplasm of eosinophils is dominated by striking eosinophilic granules (large, nearly spherical, closely packed, uniform in size). no alkaline phosphatase activity was observed in the eosinophils. a positive reaction for myeloperoxidase (faint at ph 7,0 an strong at ph 10,0) was visualized as a brown precipitate in the eosinophils. em observation revealed myeloperoxidase activity only in a few granules (at ph 7,0) and in a majority of the granules (at ph 10,0). basic protein reaction product is visible essentially at the periphery of all the citoplasmic granules in the eosinophils.
CARACTERISTICAS CITOQUIMICAS MORFOLOGICAS Y ULTRAESTRUCTURALES DE EOSINOFILOS DE Caiman crocodilus yacare (Daudin, 1802) (Reptilia, Crocodilia) MORPHOLOGICAL AND ELETRONIC CYTOCHEMISTRY OBSERVATIONS IN EOSINOPHIL IN Caiman crocodilus yacare (Daudin, 1802) (Reptilia, Crocodilia)
Luiz Waldemar Oliveira,Weber Leal de Moura,Eliana Reiko Matushima,Mizue Imoto Egami
Revista chilena de anatomía , 1998,
Abstract: RESUMEN: Muestras de sangre periférica de reptiles, jóvenes y sanos, fueron recolectadas del seno venoso cervical, en tubos que contenían EDTA 10%. Inmediatamente los frotis fueron sometidos a los siguientes procedimientos: a) Tinción con solución de Rosenfeld para el análisis morfológico. b)Detección de la fosfatasa alcalina con naftol AS-MX fosfato y mieloperoxidasa (pH 7,0 y pH10,0) con o-toluidina H(2)0(2). Una parte de la sangre con EDTA fue centrifugada por 15' a 2.000rpm y el coágulo conteniendo los leucocitos, fue dividido en dos fragmentos y tratados con: a) glutaraldehído a 2% (1h a 4C) para la reacción de la fosfatasa alcalina; b)glutaraldehído a 1,25% + paraformaldehído al 1% (1h 4C), para la reacción de la mieloperoxidasa. Los eosinófilos revelaron núcleo excéntrico redondeado, oval o lobulado con nucléolo prominente. El citoplasma de los eosinófilos es ocupado por gránulos eosinofílicos evidentes (grandes, aproximadamente esféricos, compactos, uniformes en tama o). No fue observada actividad de la fosfatasa alcalina en eosinófilos. Una reacción positiva débil en pH 7,0 y fuerte en pH 10,0, para mieloperoxidasa fue visualizada como un precipitado marrón, en los eosinófilos. El microscopio electrónico reveló actividad de la mieloperoxidasa solamente en algunos gránulos en pH 7,0 y en la mayoría de los gránulos en pH 10,0. Proteínas básicas produjeron reacción visible, principalmente en la periferia de todos los gránulos citoplasmáticos de los eosinófilos. SUMMARY: Samples of peripheral blood from healthy young reptiles were collected from the cervical venous sinus in tubes containing EDTA 10%. Freshly prepared smears were used for the following purpuses: a) Staining in Rosenfeld solution for the morphological analyses. b) Detection of alkaline phosphatase with naphthol ASMX-phosphate and myeloperoxidase (pH 7,0 and pH10,0) with o-toluidine H(2)0(2). The same blood samples with EDTA were centrifuged for 15' at 2,000rpm and the buffy coat containing leukocytes was devided in two fragments and one of them treated with glutaraldehyde (1h at 4C) and used for alkaline phosphatase reaction and the other 1,25% glutaraldehyde + 1% paraformaldehyde (1h 4C) and used for myeloperoxidase reaction. The eosinophils revealed round, oval or lobed eccentric nuclei with prominent nucleoli. The cytoplasm of eosinophils is dominated by striking eosinophilic granules (large, nearly spherical, closely packed, uniform in size). No alkaline phosphatase activity was observed in the eosinophils. A positive reaction for myeloperoxidase (faint at ph 7,0 an strong at pH
Prevalence of enterobacteria in Bothrops jararaca in S o Paulo State: microbiological survey and antimicrobial resistance standards = Prevalência de enterobactérias em Bothrops jararaca no Estado de S o Paulo: levantamento microbiológico e padr es de resistência antimicrobiana
Henrique Mar?al Bastos,Luiz Fernando Larangeira Lopes,Marco Aurélio Gattamorta,Eliana Reiko Matushima
Acta Scientiarum : Biological Sciences , 2008,
Abstract: There are few microbiological surveys on reptiles in Brazil. The study described here focuses on a species of snake of great medical interest and serves as basis for other studies and comparisons. The samples were collected directly from the colon of healthy adult Jararacas. The material was seeded in MacConkey Agar (Difco ) and XLT4 Agar (Difco ). The isolates were identified through the API 20E Identification System (BioMériux ). The isolates of Salmonella sp. were submitted to serotyping. Finally, the colonies were submitted to the antimicrobial sensitivity test. Several genera of the Enterobacteriaceae family were obtained (Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Morganella, Proteus, Providencia and Salmonella), as well as a genus of morphologically similar Gram-negative bacteria (Aeromonas). Salmonella, Citrobacter and Escherichia were the most frequent isolates. Fourteen (14) serotypes of Salmonella were identified; 13 are of the subspecies IIIb and 1 of the subspecies IV. Aeromonas, Enterobacter, Escherichia and Klebsiella presented a higher resistance rate to antibiotics. Kluyvera and Salmonella were the genera most sensitive to the tested drugs. Aminoglycosides presented good antimicrobial action, but chloramphenicol was the only drug to which no isolate presentedresistance. Existem poucos levantamentos microbiológicos em répteis no Brasil. O estudo aqui descrito enfoca uma espécie de serpente de grande interesse médico e serve como base para outros estudos e compara es. As amostras foram colhidas diretamente do cólon de jararacasadultas e saudáveis. O material foi semeado em ágar MacConkey (Difco ) e ágar XLT4 (Difco ). Os isolados foram identificados por meio do Sistema API 20E de Identifica o Bioquímica (Galerias API-BioMériux ). Os isolados de Salmonella sp. foram submetidos àsorotipagem. Por fim, as col nias foram submetidas ao teste de sensibilidade antimicrobiana. Foram obtidos vários gêneros da família Enterobacteriaceae (Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Morganella, Proteus, Providencia e Salmonella) e um gênero de bactéria Gram-negativa morfologicamente semelhante (Aeromonas). Salmonella, Citrobacter e Escherichia foram os isolados mais frequentes. 14 sorotipos de Salmonella foram identificados, sendo 13 da subespécie IIIb e 1 da subespécie IV. Aeromonas, Enterobacter, Escherichia e Klebsiella apresentaram maior taxa de resistência aos antibióticos. Kluyvera e Salmonella foram os gêneros mais sensíveis às drogas testadas. Aminoglicosídeos apresentaram boa a o antimicrobiana, mas clo
Effect of Starvation-Refeeding Status on Cholesterol Metabolism in Rats Fed High-Cholesterol Diet  [PDF]
Reiko Inai, Tatsuhiro Matsuo
Food and Nutrition Sciences (FNS) , 2011, DOI: 10.4236/fns.2011.22016
Abstract: The present study investigated the effect of starvation-refeeding status on cholesterol metabolism in rats fed a high-cholesterol diet or a cholesterol-free diet. Twenty male and 20 female Donryu rats (age 5 weeks) were fed a cho-lesterol-free diet for 14 days. Then the male and female rats were each divided into two groups: feeding and starva-tion-refeeding groups. The feeding groups were fed the experimental diet for 3 days, and the starvation-refeeding groups fasted for 2 days followed by 3 days of feeding. Half of each of groups was fed a cholesterol-free diet and the other half was fed a high-cholesterol diet. Starvation-refeeding significantly increased the plasma free cholesterol and HDL-cholesterol concentrations in both the high-cholesterol-diet-fed rats and the cholesterol-free-diet-fed rats. In the female rats, plasma total cholesterol and cholesteryl ester concentrations were significantly higher in the high-cholesterol groups than in the cholesterol-free groups, whereas TG concentration and total cholesterol/TG ratio were not significantly different among all of the groups. Liver total cholesterol and cholesteryl ester were significantly higher in the high-cholesterol groups than in the cholesterol-free groups in both male and female rats. These results suggest that starvation-refeeding affected cholesterol metabolism at least in part. The reactivity of the cholesterol me-tabolism may be different between male and female rats.
Page 1 /2477
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.