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Search Results: 1 - 10 of 353596 matches for " Eliana G. de Macedo Lemos "
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Novel Inorganic Pyrophosphatase from Soil Metagenomic and Family and Subfamily Prediction  [PDF]
Gisele R. Rodrigues, Silvana Pompeia Val-Moraes, Eliana G. de Macedo Lemos, Jo?o Martins Pizauro
Open Journal of Applied Sciences (OJAppS) , 2014, DOI: 10.4236/ojapps.2014.42008
Abstract: Inorganic pyrophosphatase (PPase) is widely studied, to be extremely important for survival of plants and microorganisms. PPases catalyze an essential reaction the hydrolysis of inorganic pyrophosphate (PPi) to inorganic phosphate (Pi). Studies involving the mechanism of PPase were performed in microorganisms culture. We didn’t found reports of PPase derived from soil meta-genomic libraries. Soil environment has immense diversity of microorganisms, yet most remains unexplored and the metagenome are the technologies used and investigate uncultured microorganisms potential. The aim is to identify novel genes using the metagenomic approaches from a bioinformatics perspective and hopefully will serve as a useful resource. With this purpose, we used the metagenomic library of Eucalyptus spp. arboretum (EAA). We did a screening to select a positive clone and submitted them to the process of shotgun. The data obtained was submitted to bioinformatics analyses. These analyses identified were the novel MetaPPase gene and were classified according to the predict family and subfamily.
Distinctively variable sequence-based nuclear DNA markers for multilocus phylogeography of the soybean- and rice-infecting fungal pathogen Rhizoctonia solani AG-1 IA
Ciampi, Maisa B.;Gale, Liane Rosewich;Lemos, Eliana G. de Macedo;Ceresini, Paulo C.;
Genetics and Molecular Biology , 2009, DOI: 10.1590/S1415-47572009005000063
Abstract: a series of multilocus sequence-based nuclear dna markers was developed to infer the phylogeographical history of the basidiomycetous fungal pathogen rhizoctonia solani ag-1 ia infecting rice and soybean worldwide. the strategy was based on sequencing of cloned genomic dna fragments (previously used as rflp probes) and subsequent screening of fungal isolates to detect single nucleotide polymorphisms (snps). ten primer pairs were designed based on these sequences, which resulted in pcr amplification of 200-320 bp size products and polymorphic sequences in all markers analyzed. by direct sequencing we identified both homokaryon and heterokaryon (i.e. dikaryon) isolates at each marker. cloning the pcr products effectively estimated the allelic phase from heterokaryotic isolates. information content varied among markers from 0.5 to 5.9 mutations per 100 bp. thus, the former rflp codominant probes were successfully converted into six distinctively variable sequence-based nuclear dna markers. rather than discarding low polymorphism loci, the combination of these distinctively variable anonymous nuclear markers would constitute an asset for the unbiased estimate of the phylogeographical parameters such as population sizes and divergent times, providing a more reliable species history that shaped the current population structure of r. solani ag-1 ia.
Molecular and Pathogenic Study of Guignardia spp. Isolates Associated to Different Hosts  [PDF]
Ester Wickert, Andressa de Souza, Rodrigo Matheus Pereira, Luciano Takeshi Kishi, Eliana Gertrudes de Macedo Lemos, Antonio de Goes
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.42016
Abstract:

Fungi of Guignardia genus are commonly isolated from different plant species and most of the time, they are characterized as endophytes. However, some species of this genus, like G. citricarpa and G. psidii are known as causal agents of serious diseases that affect important crops such as Citrus Black Spot and guava fruit rot, respectively. They are also responsible for diseases that cause foliar spots in different fruit species and also in other crops, but cause minor damages. Despite evidences that G. mangiferae colonizes different plant species, there are few studies about its genetic diversity associated with different hosts. This work has the objective to characterize Guignardia isolates obtained from different hosts and tissues by RAPD, fAFLP and DNA sequence of ITS1-5.8S-ITS2 region, as well as to develop pathogenicity tests through cross inoculation in citrus and guava fruits. It was observed that molecular markers were able to discriminate isolates of different Guignardia species. Pathogenicity tests showed that G. citricarpa caused CBS symptoms on citrus fruits, but it did not produce any symptoms in guava fruits. G. mangiferae isolates were able to cause rot symptoms on guava fruits, but they have

Composi??o de exopolissacarídeos produzidos por estirpes de rizóbios cultivados em diferentes fontes de carbono
Castellane, Tereza Cristina Luque;Lemos, Eliana Gertrudes de Macedo;
Pesquisa Agropecuária Brasileira , 2007, DOI: 10.1590/S0100-204X2007001000019
Abstract: the objective of this work was to evaluate the effect of different carbon souces on the exopolysaccharides composition produced by rhizobium tropici semia 4077 and semia 4080, and the effect of these strains inoculation on the nodulation in common bean. the carbon sources used interfered on the concentrations of the galactose and glucose of exopolysaccharides, being higher in semia 4080 strain when cultivated in sucrose medium. even though semia 4080 strain produced a large quantity of exopolysaccharides in culture, there was no difference between strains regarding the number of nodules, independently of the carbon source used for inoculum preparation. however, in the parameter dry weight of nodules there was significant difference between the strains, independently of the carbon sources used for inoculum preparation.
Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers
Kishi, Luciano Takeshi;Wickert, Ester;Lemos, Eliana Gertrudes de Macedo;
Revista Brasileira de Fruticultura , 2008, DOI: 10.1590/S0100-29452008000100037
Abstract: the first phytopathogenic bacterium with its dna entirely sequenced is being detected and isolated from different host plants in several geographic regions. although it causes diseases in cultures of economic importance, such as citrus, coffee, and grapevine little is known about the genetic relationships among different strains. actually, all strains are grouped as a single species, xylella fastidiosa, despite colonizing different hosts, developing symptoms, and different physiological and microbiological observed conditions. the existence of genetic diversity among x. fastidiosa strains was detected by different methodological techniques, since cultural to molecular methods. however, little is know about the phylogenetic relationships developed by brazilian strains obtained from coffee and citrus plants. in order to evaluate it, faflp markers were used to verify genetic diversity and phylogenetic relationships developed by brazilian and strange strains. faflp is an efficient technique, with high reproducibility that is currently used for bacterial typing and classification. the obtained results showed that brazilian strains present genetic diversity and that the strains from this study were grouped distinctly according host and geographical origin like citrus-coffee, temecula-grapevine-mulberry and plum-elm.
Caracteriza??o de rizóbios indicados para produ??o de inoculantes por meio de sequenciamento parcial do 16S rRNA
Toledo, Bethania Figueiredo Barbosa de;Marcondes, Jackson;Lemos, Eliana Gertrudes de Macedo;
Pesquisa Agropecuária Brasileira , 2009, DOI: 10.1590/S0100-204X2009000400008
Abstract: the aim of this work was to compare the partial sequences of 16s rrna gene of rhizobia strain patterns already classified with strains recommended for the production of inoculants in brazil, in order to verify the reliability of partial sequencing of the gene for the purpose of rapid identification of strains. polymerase chain reaction (pcr) sequencing using primers on the coding region of the 16s rrna gene among the bacteria studied was conducted. the results were analyzed by consulting the nucleotides' similarity based on basic local alignment search tool (blastn) and by interpreting the phylogenetic trees generated by bioinformatic tools. the taxonomic classification of semia strains recommended for legume inoculation based on morphological properties and host specificity was not confirmed in all strains. the similarity of the blastn consultation by partial sequencing of the gene found in strains studied is consistent with the classification proposed by the construction of a phylogenetic tree of sequences of strains in most cases.
Genetic variations among passion fruit species using rapd markers
Aukar, Ana Paula de Andrade;Lemos, Eliana Gertrudes de Macedo;Oliveira, Jo?o Carlos;
Revista Brasileira de Fruticultura , 2002, DOI: 10.1590/S0100-29452002000300044
Abstract: it has been evaluated the genetic variability through the use of rapd molecular markers on the following passionflower species: passiflora amethystina, p. caerulea, p. cincinnata, p. coccinea, p. serrato digitata, p. foetida, p. maliformis, p. alata, p. giberti, p. laurifolia, p. macrocarpa, p. nitida, p. setacea, p. suberosa, p. ligularis, p. capsularis, p. edulis sims and its botanical variety p. edulis sims f. flavicarpa deg. in this research work, the analyses of the random amplified polymorphic dna products (rapd) were employed to estimate the genetic diversity and the taxonomic linkage within the species above. the total of 21 primers were used in this study which generated 270 different polymorphic products. it was possible to detect that the passiflora species had shown a similarity of 17,3%, and between passiflora edulis sims and passiflora edulis sims f. flavicarpa a similarity of 34,35% has been found. the rate of similarity within edulis specie is low, making it clear that a large variability between the yellow and the purple forms exists.
Genetic variations among passion fruit species using rapd markers
Aukar Ana Paula de Andrade,Lemos Eliana Gertrudes de Macedo,Oliveira Jo?o Carlos
Revista Brasileira de Fruticultura , 2002,
Abstract: It has been evaluated the genetic variability through the use of RAPD molecular markers on the following passionflower species: Passiflora amethystina, P. caerulea, P. cincinnata, P. coccinea, P. serrato digitata, P. foetida, P. maliformis, P. alata, P. giberti, P. laurifolia, P. macrocarpa, P. nitida, P. setacea, P. suberosa, P. ligularis, P. capsularis, P. edulis Sims and its botanical variety P. edulis Sims f. flavicarpa Deg. In this research work, the analyses of the random amplified polymorphic DNA products (RAPD) were employed to estimate the genetic diversity and the taxonomic linkage within the species above. The total of 21 primers were used in this study which generated 270 different polymorphic products. It was possible to detect that the Passiflora species had shown a similarity of 17,3%, and between Passiflora edulis Sims and Passiflora edulis Sims f. flavicarpa a similarity of 34,35% has been found. The rate of similarity within edulis specie is low, making it clear that a large variability between the yellow and the purple forms exists.
Crescimento in vitro e aclimatiza??o de Cattleya loddigesii Lindley (Orchidaceae) com carv?o ativado sob dois espectros luminosos
Galdiano Júnior, Renato Fernandes;Mantovani, Cibele;Pivetta, Kathia Fernandes Lopes;Lemos, Eliana Gertrudes de Macedo;
Ciência Rural , 2012, DOI: 10.1590/S0103-84782012005000019
Abstract: the aim of this study was to evaluate the effect of different concentrations of activated charcoal in ? ms (half concentration of macronutrients) culture medium under two light spectra on the in vitro growth of cattleya loddigesii seedlings. plantlets with approximately 90 days were subcultured under eight treatments, consisting of different active charcoal concentrations (0; 0.5; 1.0 and 2.0g l-1) in ? ms medium combined with white and red light spectra. after 180 days of germination, biometric data, fresh weight, and the level of photosynthetic pigments were evaluated. plantlets acclimatized in a greenhouse were evaluated for survival after 120 days. total chlorophyll, chlorophyll a, and carotenoid concentrations were higher in treatments under white light, while red light promoted greater chlorophyll b, plantlets with fewer and shorter roots, and high ex vitro mortality. the addition of 2.0g l-1 of active charcoal to the culture medium and the use of white light provided greater development efficiency both on in vitro culture and ex vitro plant survival.
Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean
González, Tehuni Orlando;Campanharo, Jo?o Carlos;Lemos, Eliana Gertrudes de Macedo;
Pesquisa Agropecuária Brasileira , 2008, DOI: 10.1590/S0100-204X2008000900012
Abstract: this study aimed to genetically characterize four new rhizobium strains, and to evaluate their nodulation and fixation capacity compared to commercial strains and to native rhizobia population of a brazilian rhodic hapludox. two experiments were carried out in randomized blocks design, under greenhouse conditions, in 2007. in the first experiment, the nodulation and nitrogen fixation capacity of new strains were evaluated, in comparison to the commercial strains ciat-899 and prf-81 and to native soil population. it was carried out in plastic tubes filled with vermiculite. dna extractions and pcr sequencing of the intergenic space were made from the isolated pure colonies, in order to genetically characterize the strains and the native rhizobia population. in the second experiment, the nodulation and productivity of common beans perola cultivar were determined, with the use of evaluated strains, alone or in mixture with prf-81 strain. it was carried out in pots filled with soil. the native soil population was identified as rhizobium sp. and was inefficient in nitrogen fixation. three different rhizobium species were found among the four new strains. the lbmp-4br and lbmp-12br new strains are among the ones with greatest nodulation and fixation capacity and exhibit differential responses when mixed to prf-81.
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