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Search Results: 1 - 10 of 422155 matches for " Elaine M Souza-Fagundes "
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Screening and fractionation of plant extracts with antiproliferative activity on human peripheral blood mononuclear cells
Souza-Fagundes Elaine M,Queiroz Ana BR,Martins Filho Olindo Assis,Gazzinelli Giovanni
Memórias do Instituto Oswaldo Cruz , 2002,
Abstract: Three hundred and thirteen extracts from 136 Brazilian plant species belonging to 36 families were tested for their suppressive activity on phytohemaglutinin (PHA) stimulated proliferation of human peripheral blood mononuclear cells (PBMC). The proliferation was evaluated by the amount of [3H]-thymidine incorporated by the cells. Twenty extracts inhibited or strongly reduced the proliferation in a dose-dependent manner at doses between 10 and 100 μg/ml. Three of these extracts appeared to be non-toxic to lymphocytes, according to the trypan blue permeability assay and visual inspection using optical microscopy. Bioassay-guided fractionation of Alomia myriadenia extract showed that myriadenolide, a labdane diterpene known to occur in this species, could account for the observed activity of the crude extract. Using a similar protocol, an active fraction of the extract from Gaylussacia brasiliensis was obtained. Analysis of the 1H and13C NMR spectra of this fraction indicates the presence of an acetylated triterpene whose characterization is underway. The extract of Himatanthus obovatus is currently under investigation.
Effect of Arrabidaea chica extracts on the Ehrlich solid tumor development
Ana Flávia C. Ribeiro,Thalita C. Telles,Vany P. Ferraz,Elaine M. Souza-Fagundes
Revista Brasileira de Farmacognosia , 2012,
Abstract: The aim of this study was to investigate the effect of Arrabidaea chica (Humb. & Bonpl.) B. Verl., Bignoniaceae, extracts on Ehrlich solid tumor development in Swiss mice. Leaves of A. chica were extracted with two distinct solvents, ethanol and water. The phytochemical analysis of the extracts indicated different classes of secondary metabolites like as anthocyanidins, flavonoids, tannins and saponins. Ethanol (EE) and aqueous (AE) extracts at 30 mg/kg reduced the development of Ehrlich solid tumor after ten days of oral treatment. The EE group presented increase in neutrophil count, α1 and β globulin values, and decrease of α2 globulin values. Furthermore, EE reduced the percentage of CD4+ T cells in blood but did not alter the percentage of inflammatory mononuclear cells associated with tumor suggesting a direct action of EE on tumor cells. Reduced tumor development observed in AE group was accompanied by a lower percentage of CD4+ T lymphocytes in blood. At the tumor microenvironment, this treatment decreased the percentage of CD3+ T cells, especially due to a reduction of CD8+ T subpopulation and NK cells. The antitumor activity presented by the AE is possibly related to an anti-inflammatory activity. None of the extracts produced toxic effects in animals. In conclusion, the ethanol and aqueous extracts of A. chica have immunomodulatory and antitumor activities attributed to the presence of flavonoids, such as kaempferol. These effects appear to be related to different mechanisms of action for each extract. This study demonstrates the potential of A. chica as an antitumor agent confirming its use in traditional popular medicine.
Thiocyanate degradation by pure and mixed cultures of microorganisms
Souza-Fagundes, Elaine M.;Rosa, Luiz H.;Gomes, Newton C.M.;Santos, Mércia H.;Pimentel, Patricia F.;
Brazilian Journal of Microbiology , 2004, DOI: 10.1590/S1517-83822004000300012
Abstract: a mixed culture and a pure bacterial strain (bmv8) were isolated from a bioreactor for thiocyanate treatment. both cultures removed 5 mm of thiocyanate from the medium in 36 hours. the mixed culture was able to tolerate concentrations up to 60 mm. the efficiency of thiocyanate degradation decreased when the cells were immobilized.
Biological potential of Stillingia oppositifolia
Cota, Betania Barros;Johann, Susana;Oliveira, Djalma M.;Siqueira, Ezequias P.;Souza-Fagundes, Elaine M.;Cisalpino, Patrícia S.;Alves, Tania M. A.;Zani, Carlos Leomar;
Revista Brasileira de Farmacognosia , 2011, DOI: 10.1590/S0102-695X2011005000016
Abstract: organic extracts from leaves and stems of stillingia oppositifolia baill. ex müll. arg., euphorbiaceae, were screened for antifungal and cytotoxic properties. the extracts presented minimum inhibitory concentration values around 250 μg.ml-1 against candida krusei and candida tropicalis, and around 63 μg.ml-1 for paracoccidioides brasiliensis. they were tested on three human cell lines (uacc-62, mcf-7, and tk-10), disclosing gi50 values, (concentration able to inhibit 50% of the cell growth) ranging from 50 to 100 μg.ml-1. organic extract from stems furnished hexanic, dichloromethanic and aqueous phases after partition. chromatographic fractionation of the hexanic soluble phase of the stems yielded aleuritolic acid 3-acetate, β-sitosterol, 3-epi-β-amyrin, β-amyrone and palmitic acid. these compounds showed antifungal and cytotoxic activities in the same range as the organic crude extract and low toxic effect against mononuclear cells obtained from human peripheral blood. this is the first report on chemical and biological potential of s. oppositifolia.
Effect of Arrabidaea chica extracts on the Ehrlich solid tumor development
Ribeiro, Ana Flávia C.;Telles, Thalita C.;Ferraz, Vany P.;Souza-Fagundes, Elaine M.;Cassali, Geovanni D.;Carvalho, Andréa T.;Melo, Marilia M.;
Revista Brasileira de Farmacognosia , 2012, DOI: 10.1590/S0102-695X2011005000225
Abstract: the aim of this study was to investigate the effect of arrabidaea chica (humb. & bonpl.) b. verl., bignoniaceae, extracts on ehrlich solid tumor development in swiss mice. leaves of a. chica were extracted with two distinct solvents, ethanol and water. the phytochemical analysis of the extracts indicated different classes of secondary metabolites like as anthocyanidins, flavonoids, tannins and saponins. ethanol (ee) and aqueous (ae) extracts at 30 mg/kg reduced the development of ehrlich solid tumor after ten days of oral treatment. the ee group presented increase in neutrophil count, α1 and β globulin values, and decrease of α2 globulin values. furthermore, ee reduced the percentage of cd4+ t cells in blood but did not alter the percentage of inflammatory mononuclear cells associated with tumor suggesting a direct action of ee on tumor cells. reduced tumor development observed in ae group was accompanied by a lower percentage of cd4+ t lymphocytes in blood. at the tumor microenvironment, this treatment decreased the percentage of cd3+ t cells, especially due to a reduction of cd8+ t subpopulation and nk cells. the antitumor activity presented by the ae is possibly related to an anti-inflammatory activity. none of the extracts produced toxic effects in animals. in conclusion, the ethanol and aqueous extracts of a. chica have immunomodulatory and antitumor activities attributed to the presence of flavonoids, such as kaempferol. these effects appear to be related to different mechanisms of action for each extract. this study demonstrates the potential of a. chica as an antitumor agent confirming its use in traditional popular medicine.
Screening and fractionation of plant extracts with antiproliferative activity on human peripheral blood mononuclear cells
Souza-Fagundes, Elaine M;Queiroz, Ana BR;Martins Filho, Olindo Assis;Gazzinelli, Giovanni;Corrêa-Oliveira, Rodrigo;Alves, Tania MA;Zani, Carlos L;
Memórias do Instituto Oswaldo Cruz , 2002, DOI: 10.1590/S0074-02762002000800024
Abstract: three hundred and thirteen extracts from 136 brazilian plant species belonging to 36 families were tested for their suppressive activity on phytohemaglutinin (pha) stimulated proliferation of human peripheral blood mononuclear cells (pbmc). the proliferation was evaluated by the amount of [3h]-thymidine incorporated by the cells. twenty extracts inhibited or strongly reduced the proliferation in a dose-dependent manner at doses between 10 and 100 μg/ml. three of these extracts appeared to be non-toxic to lymphocytes, according to the trypan blue permeability assay and visual inspection using optical microscopy. bioassay-guided fractionation of alomia myriadenia extract showed that myriadenolide, a labdane diterpene known to occur in this species, could account for the observed activity of the crude extract. using a similar protocol, an active fraction of the extract from gaylussacia brasiliensis was obtained. analysis of the 1h and 13c nmr spectra of this fraction indicates the presence of an acetylated triterpene whose characterization is underway. the extract of himatanthus obovatus is currently under investigation.
Canine distemper virus induces apoptosis in cervical tumor derived cell lines
Helen L Del Puerto, Almir S Martins, Amy Milsted, Elaine M Souza-Fagundes, Gissandra F Braz, Barbara Hissa, Luciana O Andrade, Fabiana Alves, Daniela S Raj?o, R?mulo C Leite, Anilton C Vasconcelos
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-334
Abstract: Apoptosis is a regulated form of cell death which occurs during physiological conditions. It plays a critical role in the homeostasis of multicelular organisms, and constitutes a common pathway for cell replacement, tissue remodeling, damaged cell removal and elimination of cancer cells [1]. It is a complex process which involves the participation of caspases, activation of proapoptotic genes, and inhibition of antiapoptotic proteins. Cells undergoing apoptosis present typical morphological characteristics, including membrane blebbing, chromatin condensation, cell shrinkage and apoptotic body formation [2].Apoptosis is triggered by sequential activation of caspases, a group of cysteine proteases, and proceeds primarily through two pathways. The extrinsic pathway involves activation of caspase-8 and is initiated by ligand interaction with death receptors, while the intrinsic pathway is activated by an imbalance between proapoptotic and antiapoptotic proteins from Bcl-2 family in mitochondria and cytosol, resulting in release of cytochrome c from mitochondria, which in turn activates caspase-9 [3]. Both caspase-8 and caspase-9 activate caspase-3, which along with other effectors caspases cleave critical cellular proteins, resulting in apoptosis [3].Many viral proteins can influence the cellular pathways that control cell proliferation and apoptosis. Some viral proteins trigger apoptotic cell death, and this may be important in host defense and viral spread. In other cases, viral proteins inhibit apoptosis [4].HeLa cells, derived from a cervical tumor, encode apoptosis inhibitor proteins E6 and E7, oncoproteins expressed by high-risk human papillomavirus (HPV), among them the HPV18 type [5,6]. HPV E6 protein target p53, a tumor suppressor protein that regulates the cell cycle. The E6 protein binding to p53 causes p53 inactivation by its degradation, turning off its function [6,7]. On the other hand, the HPV E7 protein acts by binding to members of the Rb (retinoblastom
The crude latex of Euphorbia tirucalli modulates the cytokine response of leukocytes, especially CD4+ T lymphocytes
Avelar, Bethania A.;Lélis, Felipe J. N.;Avelar, Renato S.;Weber, Mathias;Souza-Fagundes, Elaine M.;Lopes, Miriam T. P.;Martins-Filho, Olindo A.;Brito-Melo, Gustavo E. A.;
Revista Brasileira de Farmacognosia , 2011, DOI: 10.1590/S0102-695X2011005000096
Abstract: the plants of the euphorbiaceae family, especially those of the genus euphorbia, are frequently used by brazilian folk communities to treat a wide variety of infectious, tumoral and inflammatory illnesses. among the species of this genus, euphorbia tirucalli l. is widely used in some brazilian regions, such as the jequitinhonha river valley. there is evidence that the latex produced by e. tirucalli has antiviral and antitumor activities, but little is known about the mechanisms involved in these effects. it is likely that the mechanism for such activities involves leukocyte activation and cytokine production. in this work, we aimed to evaluate the production of type 1 (tnf-α and ifn-γ) and type 2 (il-4 and il-10) cytokines by circulating leukocyte subsets submitted to brief stimulation with the crude latex of e. tirucalli. peripheral blood leukocytes of twenty healthy subjects were submitted to 4 h incubation with crude e. tirucalli latex diluted in dimethylsulfoxide. after the incubation period, the cells were stained with fitc-conjugated monoclonal antibodies specific to the cell surface receptors cd4, cd8 and cd14, and to pe-conjugated monoclonal antibodies specific to the cytokines tnf-α, ifn-γ, il-4 and il-10. the acquisition and analysis of data were performed by flow cytometry. the results showed a significant increase (p<0.05) in the percentage of cd4+ t lymphocytes positive for the type 1 cytokines tnf-α and ifn-γ. neutrophils and cd8+ t lymphocytes showed a mixed profile of cytokine production, characterized by an increase in the percentage of cells expressing ifn-γ, tnf-α, and il-10. the data indicate a predominant type 1 cytokine response. the findings presented suggest that the effect popularly attributed to e. tirucalli usage may be attributed to its effect on the production of tnf-α and ifn-γ. however, the relationship between the in vitro and in vivo effects of e. tirucalli needs to be investigated.
Synthesis and in Vitro Cytotoxic Activity of Compounds with Pro-Apoptotic Potential
Gisell Apicela Soares,Renata Barbosa de Oliveira,Saulo Fernandes de Andrade,Ricardo José Alves,Carlos Leomar Zani,Elaine Maria De Souza-Fagundes
Molecules , 2010, DOI: 10.3390/molecules15010012
Abstract: In our search for new anticancer therapies, some compounds synthesized in our lab were selected and their potential cytotoxic activity was evaluated in vitro against two cancer cells lines including a solid tumor (UACC-62, melanoma) and a human lymphoma (JURKAT). Compounds showing cytotoxic activity were subjected to an apoptosis assay. Two compounds showed promising results.
Leishmanicidal activities of the extract from Blepharocalyx salicifolius (Kunth) O. Berg, Myrtaceae
Siqueira, Ezequias Pessoa de;Souza-Fagundes, Elaine Maria de;Sobral, Marcos Eduardo Guerra;Alves, Tania Maria de Almeida;Rabello, Ana;Zani, Carlos Leomar;
Revista Brasileira de Farmacognosia , 2010, DOI: 10.1590/S0102-695X2010000300020
Abstract: crude ethanolic extracts from blepharocalyx salicifolius (kunth) o. berg, myrtaceae, was fractioned by gel permeation chromatography, using sephadextm lh-20 gel. sixteen fractions were obtained and were supplied to cytotoxicity in vitro assay against leishmania (l.) amazonensis amastigota cells. it was observed eight cytotoxic fractions against leishmania (l.) amazonensis amastigota cells at range of 19 to 29 μg.ml-1. two of them were not citotoxic against human peripheral blood mononuclear cell, with a great potential to isolation of more selective leishmanicidal substances.
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