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Search Results: 1 - 10 of 71743 matches for " Edna Maria Vissoci;Bortoliero "
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Seroprevalence for hepatitis E virus (HEV) infection among volunteer blood donors of the Regional Blood Bank of Londrina, State of Paraná , Brazil
Bortoliero, André Luiz;Bonametti, Ana Maria;Morimoto, Helena Kaminami;Matsuo, Tiemi;Reiche, Edna Maria Vissoci;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2006, DOI: 10.1590/S0036-46652006000200006
Abstract: a cross-sectional study was carried out among 996 volunteer blood donors enrolled from may 1999 to december 1999 to determine the seroprevalence of hepatitis e virus (hev) infection among volunteer blood donors of the regional blood bank of londrina, state of paraná, brazil, and to evaluate whether the rate of seroprevalence of igg anti-hev antibodies is associated with sociodemographic variables and with seropositivity for hepatitis a virus (hav) infection. all participants answered the questionnaire regarding the sociodemographic characterisitcs. serum samples were tested for igg antibodies to hev (anti-hev) by an enzyme linked immunoassay (elisa). all serum samples positive for anti-hev igg and 237 serum samples negative for anti-hev were also assayed for igg anti-hav antibodies by elisa. anti-hev igg was confirmed in 23/996 samples, resulting in a seroprevalence of 2.3% for hev infection, similar to previous results obtained in developed countries. no significant association was found between the presence of anti-hev igg antibodies and the sociodemographic variables including gender, age, educational level, rural or urban areas, source of water, and sewer system (p > 0.05). also, no association with seropositivity for anti-hav igg antibodies was observed (p > 0.05). although this study revealed a low seroprevalence of hev infection in the population evaluated, the results showed that this virus is circulating among the population from londrina, south brazil, and point out the need of further studies to define the clinical and epidemiological importance of hev infection and to identify additional risk factors involved in the epidemiology and pathogenesis of this infection in this population.
Evaluation of surrogate markers for human immunodeficiency virus infection among blood donors at the blood bank of "Hospital Universitário Regional Norte do Paraná", Londrina, PR, Brazil
Reiche Edna Maria Vissoci,Vogler Ingridt Hildegard,Morimoto Helena Kaminami,Bortoliero André Luis
Revista do Instituto de Medicina Tropical de S?o Paulo , 2003,
Abstract: This study evaluated the usefulness of the anti-HBc, hepatitis C virus antibodies (anti-HCV), human T cell lymphotropic virus I and II antibodies (anti-HTLV I/II), serologic tests for syphilis, and surface antigen of hepatitis B virus (HBsAg) as surrogate markers for the risk for HIV infection in 80,284 serum samples from blood donors from the Blood Bank of "Hospital Universitário Regional Norte do Paraná", Londrina, Paraná State, Brazil, analyzed from July 1994 to April 2001. Among 39 blood donors with positive serology for HIV, 12 (30.8%) were anti-HBc positive, 10 (25.6%) for anti-HCV, 1 (2.6%) for anti-HTLV I/I, 1 (2.6%) was positive for syphilis, and 1 (2.6%) for HBsAg. Among the donors with negative serology for HIV, these markers were detected in 8,407 (10.5%), 441 (0.5%), 189 (0.2%), 464 (0.6%), and 473 (0.6%) samples, respectively. The difference was statistically significant (p < 0.001) for anti-HBc and anti-HCV. Although the predictive positive value for these surrogate markers were low for HIV infection, the results confirmed the anti-HBc and anti-HCV as useful surrogate markers for HIV infection thus reinforcing the maintenance of them in the screening for blood donors contributing to the prevention of the small number of cases in which HIV is still transmitted by transfusion.
Serological, epidemiological and molecular aspects of hepatitis C virus infection in a population from Londrina, PR, Brazil, 2001-2002
Vogler, Ingridt Hildegard;Nishiya, Anna;Morimoto, Helena Kaminami;Reiche, Edna Maria Vissoci;Bortoliero, André Luiz;Matsuo, Tiemi;Sabino, Ester Cerdeira;Vaz, Adelaide Jose;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2004, DOI: 10.1590/S0036-46652004000600002
Abstract: serological, epidemiological and molecular aspects of hepatitis c virus (hcv) infection were evaluated in 183 subjects from londrina, paraná, brazil, and adjacent areas. serum samples which tested anti-hcv positive by microparticle enzyme immunoassay (meia) obtained from eight patients with chronic hepatitis c, 48 blood donors, and 127 patients infected with the human immunodeficiency virus (hiv) were submitted to another enzyme immunoassay (elisa) and to the polymerase chain reaction (pcr). about 78.7% of samples were also reactive by elisa, with the greater proportion (70.8%) of discordant results verified among blood donors. a similar finding was observed for hcv-rna detection by pcr, with 111/165 (67.3%) positive samples, with higher rates among hiv-positive subjects and patients with chronic hepatitis than among blood donors. sixty-one pcr-positive samples were submitted to hcv genotyping, with 77.1, 21.3 and 1.6% of the samples identified as types 1, 3 and 2, respectively. finally, analysis of some risk factors associated with hcv infection showed that intravenous drug use was the most common risk factor among hiv/hcv co-infected patients, while blood transfusion was the most important risk factor in the group without hiv infection. the present study contributed to the knowledge regarding risk factors associated with hcv infection and the distribution of hcv genotypes in the population evaluated.
Evaluation of surrogate markers for human immunodeficiency virus infection among blood donors at the blood bank of "Hospital Universitário Regional Norte do Paraná", Londrina, PR, Brazil
Reiche, Edna Maria Vissoci;Vogler, Ingridt Hildegard;Morimoto, Helena Kaminami;Bortoliero, André Luis;Matsuo, Tiemi;Yuahasi, Kátia Kioko;Cancian, Sanderson Júnior;Koguichi, Roberto Setsuo;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2003, DOI: 10.1590/S0036-46652003000100005
Abstract: this study evaluated the usefulness of the anti-hbc, hepatitis c virus antibodies (anti-hcv), human t cell lymphotropic virus i and ii antibodies (anti-htlv i/ii), serologic tests for syphilis, and surface antigen of hepatitis b virus (hbsag) as surrogate markers for the risk for hiv infection in 80,284 serum samples from blood donors from the blood bank of "hospital universitário regional norte do paraná", londrina, paraná state, brazil, analyzed from july 1994 to april 2001. among 39 blood donors with positive serology for hiv, 12 (30.8%) were anti-hbc positive, 10 (25.6%) for anti-hcv, 1 (2.6%) for anti-htlv i/i, 1 (2.6%) was positive for syphilis, and 1 (2.6%) for hbsag. among the donors with negative serology for hiv, these markers were detected in 8,407 (10.5%), 441 (0.5%), 189 (0.2%), 464 (0.6%), and 473 (0.6%) samples, respectively. the difference was statistically significant (p < 0.001) for anti-hbc and anti-hcv. although the predictive positive value for these surrogate markers were low for hiv infection, the results confirmed the anti-hbc and anti-hcv as useful surrogate markers for hiv infection thus reinforcing the maintenance of them in the screening for blood donors contributing to the prevention of the small number of cases in which hiv is still transmitted by transfusion.
Módulos instrucionais: uma experiência no ensino de imunologia clinica
Edna Maria Vissoci Reiche
Semina : Ciências Sociais e Humanas , 1985, DOI: 10.5433/
Abstract: The aim of this study was to appraise the use of modular instruction and its effect in terms of academic performance, student’s initiative and participation for 10 students of clinical immunology in the Pharmacy-Biochemical course on the Universidade Estadual de Londrina, PR. The results showed an academic performance equal or even superior to the minimum expected standard of 80% as stipulated by the researches. The students also revealed significant increases in levels of initiative and participation reaching minimum grades of 51.3% and 73.8%. It is concluded that modular instruction will eventually prove to be an individualized efficient method, proper for the teaching of clinical immunology. O estudo envolveu a testagem de um modelo de ensino individualizado - instru o modular - em dez alunos da disciplina de Imunologia Clínica, do curso de Farmácia e Bioquímica da Universidade Estadual de Londrina. Teve como objetivos avaliar a utiliza o da instru o modular e seus efeitos, em termos de rendimento acadêmico, iniciativa e participa o dos alunos, bem como verificar a opini o dos mesmos em rela o à instru o modular e também correlacionar a variável rendimento acadêmico com as variáveis iniciativa e participa o dos alunos, quando submetidos ao ensino da Imunologia Clínica, através da instru o modular. Os resultados evidenciaram um rendimento acadêmico igual ou superior ao critério mínimo de 80%, estipulado pela pesquisadora. Ao mesmo tempo, os alunos demonstraram níveis significativos de iniciativa e participa o, tendo como valores mínimos 51,3% e 73,8%, respectivamente. Conclui-se que a instru o modular mostrou serum método de ensino individualizado eficiente para o ensino da disciplina de Imunologia Clínica.
Soroprevalência de anticorpos antivírus da hepatite C em doadores de sangue, Brasil
Paltanin,Lindamyr Fornazieri; Reiche,Edna Maria Vissoci;
Revista de Saúde Pública , 2002, DOI: 10.1590/S0034-89102002000400004
Abstract: objective: to determine the seroprevalence of anti-hepatitis c virus antibodies (anti-hcv) in blood donors, and to describe the correlation between screening serological test results and confirmatory test. methods: epidemiological and laboratorial records of 10,090 blood donors of the blood unit in the city of apucarana, brazil, from january 1997 to december 1999 were assessed. anti-hcv serum antibodies were detected using enzyme-linked immunosorbent assay (elisa). serum reactive samples were tested using riba (recombinant immunoblot assay). statistical analysis was performed using chi-square test, fischer's test and kappa index of agreement. results: the results showed that of all the donors, 2,461 (24.4%) were females, 7,629 (75.6%) were males, with ages ranging from 18 to 65 years old. of 10,090 serum samples tested using elisa, 88 were reactive to anti-hcv, a seroprevalence of 0.9% that showed no association with either age groups (p=0.197) or sex (p=0.323). when the samples were tested using riba, 11 (12.5%) were positive, 14 (15.9%) were indeterminate, and 38 (43.2%) were negative. statistical analysis revealed a high correlation (kappa index 0.939) between elisa and riba test results. poorly reactive samples in elisa showed a high correlation with negative results in riba, and samples highly reactive in elisa showed a high correlation with positive results in riba. conclusions: the results stress the need of confirmatory tests for all anti-hcv reactive samples in screening tests. hvc infection confirmation is paramount for clinical, laboratorial, and histological evaluation of blood donors.
Soroprevalência de anticorpos antivírus da hepatite C em doadores de sangue, Brasil
Paltanin Lindamyr Fornazieri,Reiche Edna Maria Vissoci
Revista de Saúde Pública , 2002,
Abstract: OBJETIVOS: Determinar a soroprevalência de anticorpos antivírus da hepatite C (VHC) em doadores de sangue e correlacionar os resultados obtidos nos testes sorológicos de triagem e no teste confirmatório. MéTODOS: Foram analisados os registros epidemiológicos e laboratoriais de 10.090 doadores de sangue do Hemonúcleo de Apucarana, Paraná, Brasil, do período de janeiro de 1997 a dezembro de 1999. Utilizou-se o método enzimaimunoensaio (ELISA) para detec o de anticorpos anti-VHC no soro. As amostras de soro com reatividade no ELISA foram avaliadas pelo teste confirmatório RIBA (recombinant immunoblot assay). Para análise estatística, utilizaram-se os testes qui-quadrado, teste exato de Fisher e índice de Kappa. RESULTADOS: Os resultados mostraram que 2.461 (24,4%) pessoas da amostra eram do sexo feminino e 7.629 (75,6%), do sexo masculino, com idade variando de 18 a 65 anos. Das 10.090 amostras de soro analisadas pelo ELISA, 88 apresentaram positividade, revelando soroprevalência de 0,9%, n o demonstrando associa o com as diferentes faixas etárias (p=0,197) e com o sexo (p=0,323). Avaliadas pelo teste confirmatório RIBA, 11 amostras (12,5%) apresentaram resultado positivo; 14 (15,9%), resultado indeterminado; e 38 (43,2%), resultado negativo. A análise estatística revelou alta concordancia (índice Kappa de 0,939) entre os resultados obtidos no teste de ELISA e os obtidos no teste confirmatório. Amostras que forneceram resultado fracamente reagente no teste de ELISA apresentaram alta concordancia com resultado negativo no RIBA immunoblot, e amostras que forneceram resultado fortemente reagente no teste de ELISA apresentaram alta concordancia com o resultado positivo no RIBA. CONCLUS ES: Os resultados refor am a necessidade de confirma o de todos os resultados reagentes nos testes de triagem sorológica para pesquisa de anticorpo anti-VHC, uma vez que a confirma o da infec o pelo VHC é de extrema importancia para o acompanhamento clínico, laboratorial e histológico dos doadores de sangue.
Prevalência da infec o por Trypanosoma Cruzi, Treponema Pallidum e vírus da hepatite b, no período de 1980 a 1983, avaliada através de testes sorológicos, em 1977 candidatos a doadores de sangue de 33 cidades do estado do Paraná
Rubens Pontello,Edna Maria Vissoci Reiche,Edilson Jo?o Cabrera
Semina : Ciências Sociais e Humanas , 1985, DOI: 10.5433/
Abstract: In the serum from 1977 candidates to blood donors from 33 cities in the State of Paraná, Brazil, from January, 1980 to December, 1983, immunological tests were carried out for the diagnosis of infections caused by Tripanosoma cruzi, Treponema Pallidum and Hepatitis B virus. The rates of positivity obtained were: 7,2% for american tripanosomiasis, 0,7% for syphilis and 0,6% for virus B hepatitis. No soro de 1977 candidatos a doadores de sangue de 33 cidades do Estado do Paraná, Brasil, no período de janeiro de 1980 a dezembro de 1983, realizaram-se testes sorológicos para o diagnóstico de infec es causadas por Trypanosoma cruzi, Treponema pallidum e vírus da hepatite B. As seguintes taxas de positividade foram obtidas: 7,2% para tripanossomíase americana; 0,7% para sífilis e 0,6% para hepatite B.
Prevalência de tripanossomíase americana, sífilis, toxoplasmose, rubéola, hepatite B, hepatite C e da infec o pelo vírus da imunodeficiência humana, avaliada por intermédio de testes sorológicos, em gestantes atendidas no período de 1996 a 1998 no Hospital Universitário Regional Norte do Paraná (Universidade Estadual de Londrina, Paraná, Brasil)
Reiche Edna Maria Vissoci,Morimoto Helena Kaminami,Farias Grazieli Nogueira,Hisatsugu Kátia Regina
Revista da Sociedade Brasileira de Medicina Tropical , 2000,
Abstract: Com o objetivo de determinar a soroprevalência de tripanossomíase americana, sífilis, toxoplasmose, rubéola, hepatite B, hepatite C e infec o pelo vírus da imunodeficiência humana em gestantes atendidas no Hospital Universitário Regional Norte do Paraná, da Universidade Estadual de Londrina, Paraná, foi realizado estudo retrospectivo dos resultados dos testes sorológicos efetuados no período de junho de 1996 a junho de 1998. As taxas de positividade encontradas foram: 0,9% para tripanossomíase americana, 1,6% para sífilis, 67% (IgG) e 1,8% (IgM) para toxoplasmose, 89% (IgG) e 1,2% (IgM) para rubéola, 0,8% para hepatite B (AgHBs), 0,8% para hepatite C e 0,6% para infec o pelo vírus da imunodeficiência humana. Observou-se associa o entre o aumento da soroprevalência de tripanossomíase americana com a idade das gestantes (p = 0,006). Os resultados reafirmam a importancia da realiza o destes testes sorológicos no atendimento pré-natal, com a finalidade de realizar o diagnóstico e, eventualmente, adotar medidas para prevenir a transmiss o congênita ou perinatal dessas doen as.
Difficulties in the diagnosis of HTLV-2 infection in HIV/AIDS patients from Brazil: comparative performances of serologic and molecular assays, and detection of HTLV-2b subtype
Morimoto, Helena Kaminami;Morimoto, Arilson Akira;Reiche, Edna Maria Vissoci;Ueda, Luiz Toshio;Matsuo, Tiemi;Reiche, Fernando Vissoci;Caterino-de-Araujo, Adele;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2007, DOI: 10.1590/S0036-46652007000400006
Abstract: the current diagnosis of human t-lymphotropic virus type-2 (htlv-2) infection is based on the search of specific antibodies; nevertheless, several studies conducted in brazil pointed deficiencies of the commercially available kits in detecting htlv-2, mostly in hiv/aids patients. this study searched for the presence of htlv-1 and -2 in 758 hiv/aids patients from londrina, paraná, brazil. serum samples were screened for htlv-1/2 antibodies using two eia kits (vironostika and murex), and confirmed by wb (htlv blot 2.4, genelabs). the results obtained by eia disclosed 49 (6.5%) reactive sera: 43 positive by both eia kits, and six with discordant results. wb confirmed htlv-1 infection in seven samples (0.9%) and htlv-2 in 21 sera (2.8%). negative and indeterminate results were detected in four (0.5%) and 16 (2.1%) sera, respectively. blood from 47 out of 49 htlv seroreactive patients were collected and analyzed for the presence of env, ltr and tax genomic segments of htlvs by pcr. pcr confirmed six cases of htlv-1 and 37 cases of htlv-2 infection (14 out of 16 that were found to be wb indeterminate). restriction analysis of the env pcr products of htlv-2 disclosed 36 isolates of htlv-2a/c subtype, and one of htlv-2b subtype. these results emphasize the need of improving serologic tests for detecting truly htlv-2 infected patients from brazil, and confirm the presence of htlv-2b subtype in the south of this country.
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