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Search Results: 1 - 10 of 463889 matches for " Edna A.;Dutra "
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Sensibilidade toxicológica e especificidade do teste de microfixa??o de complemento na detec??o de toxinas botulínicas C e D em meio de cultura e fígado de camundongos
Menegucci, Edna A.;Dutra, Iveraldo S.;D?bereiner, Jürgen;
Pesquisa Veterinária Brasileira , 1998, DOI: 10.1590/S0100-736X1998000200001
Abstract: the toxicological sensitivity and specificity of the micro-complement fixation test (mcf) for the detection of botulinum toxins c and d were studied in supernatants of the bacterial cultures and in livers of mice inoculated with lethal and sublethal doses. botulinum toxins c and d were produced in hemoline culture medium, titered through the determination of ld50 by the mouse test and adjusted to dilutions of 10, 1, 0.1, 0.01 and 0.001 ld50. two experimental models were used to determine the toxicological sensitivity of mcf in the supernatant of the culture medium with the dilutions described, and also in liver extracts of mice weighing 20 g and inoculated with the same dilutions. detection of the botulinum toxins was attempted in liver extracts of mice which had received lethal doses of the respective toxins, and in others which had been inoculated with sublethal doses and were sacrificed in intervals of 5 days. the results show that the toxicological sensitivity of mcf, regarding the two types of toxins at the level of 0.001 ld50, was 100% when the supernatants of the culture medium were tested; this means that the sensitivity was 100 times higher than with the mouse test. the toxicological sensitivity of mcf in the liver extracts of mice inoculated with 1 and 10 ld50 of botulinum toxins c and d was inferior, giving values of 100, 80, 89 and 72% respectively. by this test it was also possible to detect botulinum toxins type c and d in liver extracts of mice inoculated with sublethal doses, up to 15 days after the injection. the specificity of mcf was 88% and 92%, when liver extracts of healthy control mice were tested and when challenged with antitoxins c and d; and 100% when challenged with the supernatant of the culture medium. these results indicate that mcf could be of importance for research and could substitute in vivo tests.
Sensibilidade toxicológica e especificidade do teste de microfixa o de complemento na detec o de toxinas botulínicas C e D em meio de cultura e fígado de camundongos
Menegucci Edna A.,Dutra Iveraldo S.,D?bereiner Jürgen
Pesquisa Veterinária Brasileira , 1998,
Abstract: No presente estudo pretendeu-se verificar a sensibilidade toxicológica e especificidade do Teste de Microfixa o de Complemento (MCF) na detec o de toxinas botulínicas C e D no sobrenadante de cultivos bacterianos e em fígados de camundongos inoculados com doses letais e subletais. As toxinas foram produzidas em meio de cultura Hemoline, tituladas através da determina o da DL50 pelo Bioensaio em Camundongo e diluídas nas concentra es de 10, 1, 0,1, 0,01 e 0,001 DL50. Desta forma, foram utilizadas em dois modelos experimentais, onde foi determinada a sensibilidade toxicológica do MCF no sobrenadante do meio de cultura com as dilui es descritas acima e ainda em extratos hepáticos de camundongos com peso corporal de 20g, inoculados com as mesmas dilui es. A tentativa de evidencia o das toxinas botulínicas nos extratos hepáticos de camundongos foi realizada através da sua extra o após a morte pela administra o das doses letais e ainda pelo sacrifício dos animais inoculados com doses subletais, em intervalos de 5 dias. Os resultados evidenciaram uma sensibilidade toxicológica para o MCF de 100% para os dois tipos de toxinas ao nível de 0,01 DL50, quando testados os sobrenadantes de meio de cultura, portanto 100 vezes superior ao Bioensaio em Camundongo. A sensibilidade toxicológica do MCF, quando examinados extratos hepáticos de camundongos inoculados com 1 e 10 DL50 de toxinas botulínicas C e D, foi inferior, com valores de 100, 80, 89 e 72%, respectivamente. Pelo teste foi possível detectar toxinas botulínicas tipos C e D nos extratos hepáticos de camundongos inoculados com doses subletais até 15 dias após a sua inocula o. A especificidade do MCF foi de 88 e 92%, quando testados extratos hepáticos de camundongos sadios, e confrontados com as antitoxinas C e D; e 100% no sobrenadante do meio de cultura. Os resultados apontam para uma possível utiliza o do teste como importante instrumento de pesquisa e ainda na eventual substitui o dos testes in vivo pelas suas implica es éticas e limita es práticas.
HISTOPATHOLOGY OF THE TELENCEPHALON AND DIENCEPHALON OF T1LAPIA NILOTICA EXPOSED TO SUBLETHAL DOSE OF MALATHION S-[1,2-DI-(ETHOXYCARBONYL ETHYL) DIMETHYL PHOSPHOROTHIOLOTHIONATE]
EDNA A. AMPARADO
BIOTROPIA : the Southeast Asian Journal of Tropical Biology , 1992,
Abstract: A 35-day exposure of Tilapia nilotica embryos to sublethal doses of 3.0 ppm and 0.3 ppm malathion, S-[l,2-di-(ethoxycarbonyl ethyl) dimethyl phosphorothiolothionate], commercial grade, EC 57, produces cellular and ultrastructure changes in the brain. A number of nuclear centers of the treated animals are markedly larger than those of the control. Aberrant features observed in day-45 embryos are the neoplastic masses and increased vascularization. Ultrastructure defects include the presence of nuclear blebs, cytoplasmic vacuolations and increased lysosomal bodies.
Reproductive Components of Safflower Genotypes Submitted of Bulk Density Levels in the Brazilian Cerrado  [PDF]
Juliana Terezinha Sasso Paludo, Edna Maria Bonfim-Silva, Tonny José Araújo da Silva, Maurício Dutra Zanotto, William Fenner, Marcio Koetz
American Journal of Plant Sciences (AJPS) , 2017, DOI: 10.4236/ajps.2017.89139
Abstract: Nutrient absorption in crops can decline and their development can be hindered by increased bulk density. This study aimed at assessing the manner in which bulk density levels affect the reproductive structures of the safflower genotypes in the Brazilian Cerrado. The completely randomized design was adopted with four replications for the experiment, which was conducted in a greenhouse using Oxisol collected from 0.0 to 0.2 m depth from the region supporting Cerrado vegetation. The treatments included ten safflower genotypes (PI 237538, PI 248385, PI 250196, PI 301049, PI 305173, PI 305205, PI 306520, PI 306603, PI 560202 and PI 613366) and five bulk density levels (1.0, 1.2, 1.4, 1.6 and 1.8 Mg·m-3). Evaluations were done at 90 days after emergence, in terms of the number, diameter and dry mass of the heads. The data were submitted to the analysis of variance. The means were grouped using the Scott-Knott test at 5% probability. The diameter and dry mass of the chapters were influenced by the mean bulk density of 1.10 Mg·m-3. A notable interaction was evident between the safflower genotypes and bulk density levels for the diameter and dry mass of the head alone, revealing the high degree of genetic variability that environmental changes induce among the genotypes. The PI 250196, PI 301049, PI 305173 and PI 305205 genotypes exhibited greater stability to the bulk density variations compared with the others. Mean bulk density of 1.2 Mg·m-3 was found to impair the development of the reproductive components of the safflower genotypes.
Ser mulato em Portugal nos primórdios da época moderna
Dutra, Francis A.;
Tempo , 2011, DOI: 10.1590/S1413-77042011000100005
Abstract: studies the cases of twenty-seven men awarded knighthoods in the portuguese military orders of christ, santiago and avis from the early seventeenth century to 1731, where witnesses in the background investigation had testified that the parents or grandparents of these men had been described as "mulatos" and/or descended from african slaves. almost all the cases involved people who were living in portugal. with one exception, the needed dispensation was for "falta de qualidade" and not for "pureza de sangue." more than 80% received dispensations for being "mulato" and became members of the order of christ (the majority) or the order of santiago
A liberdade é um fato natural ou uma constru o social?
Luiz Henrique A. Dutra
Principia : an International Journal of Epistemology , 2000,
Abstract: Discussion
Quantum propagator for some classes of three-dimensional three-body systems
A. de Souza Dutra
Physics , 2007, DOI: 10.1016/j.aop.2005.09.007
Abstract: In this work we solve exactly a class of three-body propagators for the most general quadratic interactions in the coordinates, for arbitrary masses and couplings. This is done both for the constant as the time-dependent couplings and masses, by using the Feynman path integral formalism. Finally the energy spectrum and the eigenfunctions are recovered from the propagators.
Strong-Weak Coupling Duality in Quantum Mechanics
A. de Souza Dutra
Physics , 1996,
Abstract: We present a strong-weak coupling duality for quantum mechanical potentials. Similarly to what happens in quantum field theory, it relates two problems with inverse couplings, leading to a mapping of the strong coupling regime into the weak one, giving information from the nonperturbative region of the parameters space. It can be used to solve exactly power-type potentials and to extract deep information about the energy spectra of polynomial ones.
Mapping deformed hyperbolic potentials into nondeformed ones
A. de Souza Dutra
Physics , 2005, DOI: 10.1016/j.physleta.2005.02.075
Abstract: In this work we introduce a mapping between the so called deformed hyperbolic potentials, which are presenting a continuous interest in the last few years, and the corresponding nondeformed ones. As a consequence, we conclude that these deformed potentials do not pertain to a new class of exactly solvable potentials, but to the same one of the corresponding nondeformed ones. Notwithstanding, we can reinterpret this type of deformation as a kind of symmetry of the nondeformed potentials.
Evolución histórica de los rendimientos educativos en México: 1987-2004
Villarreal Peralta, Edna María;
Estudios sociales (Hermosillo, Son.) , 2008,
Abstract: this paper examines the impact of educational level on income wages in mexico, for the period 1987-2004. for this reason, we assess the historical evolution of educational returns using data from the national survey of urban employment (eneu). also, we estimate three alternative models: first, taking years of study as a major explanatory variable; secondly, incorporating variable dummies for kinship and marital status; and, thirdly, considering educational levels. the results obtained show that the highest marginal returns correspond to individuals with higher educational levels. on the other hand, when getting the yields by gender, it seems that those of women surpass men's. finally, a sharp decline in returns during periods of economic crisis, can be observed.
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