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Search Results: 1 - 10 of 182212 matches for " Edeltrudes de Oliveira; "
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Atividade antifúngica e cinética de morte microbiana de extratos obtidos de Streptomyces spp. isolados de solos paraibanos
de Oliveira, Thompson Lopes;Lima, Edeltrudes de Oliveira;de Souza, Ivone Antonia;Cornejo, Luis Conrado Z.;
Revista Brasileira de Farmacognosia , 2010, DOI: 10.1590/S0102-695X2010000100013
Abstract: sixty eight samples were collected in different soils of paraiba, with the isolation of forty nine strains of streptomyces spp. after screening antimicrobial followed the method of diffusion in solid medium with agar block, the extracts were prepared from microorganism producers of bioactive metabolites, respectively strains sp1 and sp3, and then evaluated for antifungal activity against strains of filamentous fungi of clinical origin and atcc. the antagonism was determined through testing of diffusion disc in solid medium, microdilution and kinetics of microbial death. the inhibition zones obtained from extracts of sp-1 and sp-3 showed antagonistic effect with values greater than the halos of inhibition promoted by the drug control, often used in antifungal therapy. the results of minimum inhibitory concentrations in microdilution were significant with fungicide values ranging between 10 mg and 0.078125 mg. in the kinetics of microbial death, the activities of the extracts sp-1 and sp-3 resulted in statistically significant data front of strains tested.
Tinea capitis em Jo?o Pessoa: vis?o socioecon?mica
Aquino, Patrícia Marques Lima Pessoa de;Lima, Edeltrudes de Oliveira;Farias, Nilma Maria Porto de;
Anais Brasileiros de Dermatologia , 2003, DOI: 10.1590/S0365-05962003000600006
Abstract: background: the distribution of dermatophyte species varies according to time and place, and shows the social and economical conditions of the population. objective: this study was undertaken to determine the dermatophyte species in relation to the sex, age and race of patients with tinea capitis in paraiba state, brazil. a comparison was established with disease data from the most affluent regions of brazil. method: we studied the mycological examinations and clinical variants of 82 patients with tinea capitis in jo?o pessoa, paraiba state. results: the frequency of tinea capitis corresponded to 64.6% of clinical suspicion. t. rubrum was the most frequently isolated dermatophyte (37.7%), followed by t. tonsurans (28.3%), m. canis (24.5%), t. verrucosum (7.5%) and t. mentagrophytes (1.9%). as for sex, no predilection was found. the highest incidence was in the 0 to 10-year-old age group. 71.7% of the patients were caucasian. conclusion: comparing our results with previous publications from the southeast region, the authors highlight the differences arising from social and economical variants in the disease epidemiology.
Effect of cinnamomum zeylanicum blume essential oil on the rowth and morphogenesis of some potentially pathogenic Aspergillus species
Carmo, Egberto Santos;Lima, Edeltrudes de Oliveira;Souza, Evandro Leite de;Sousa, Frederico Barbosa de;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000100021
Abstract: cinnamomum zeylanicum blume is known for a wide range of medicinal properties. this study aimed to assess the interference of c. zeylanicum essential oil on the growth and morphogenesis of some potentially pathogenic aspergillus species. the essential oil presented strong antifungal effect causing the growth inhibition of the assayed strains and development of large growth inhibition zones. mic50 and mic90 values were 40 and 80 μl/ml, respectively. 80, 40 and 20 μl/ml of the oil strongly inhibited the radial mycelial growth of a. niger, a. flavus and a. fumigatus along 14 days. 80 and 40 μl/ml of the oil caused a 100% inhibition of the fungal spore germination. main morphological changes observed under light microscopy provided by the essential oil in the fungal strains were decreased conidiation, leakage of cytoplasm, loss of pigmentation and disrupted cell structure indicating fungal wall degeneration. it is concluded that c. zeylanicum essential oil could be known as potential antifungal compound, particularly, to protect against the growth of aspergillus species.
Tinea capitis em Jo o Pessoa: vis o socioecon mica
Aquino Patrícia Marques Lima Pessoa de,Lima Edeltrudes de Oliveira,Farias Nilma Maria Porto de
Anais Brasileiros de Dermatologia , 2003,
Abstract: FUNDAMENTOS: A distribui o das espécies de dermatófitos varia ao longo do tempo e de acordo com a regi o, refletindo as condi es socioecon micas da popula o OBJETIVOS: Estudar a Tinea Capitis quanto ao agente etiológico, ao sexo, à idade e ao grupo étnico dos doentes em Jo o Pessoa, PB, Brasil e salientar a importancia socioecon mica desses dados, comparando-os aos de regi es mais ricas do país. MéTODOS: Foram avaliados o perfil e os exames micológicos direto e cultura de 82 pacientes com suspeita clínica de T. capitis em Jo o Pessoa. RESULTADOS: A freqüência de T. capitis incluiu 64,6% das suspeitas clínicas. O dermatófito isolado com maior freqüência foi o T. rubrum (37,7%), seguido por T. tonsurans (28,3%), M. canis (24,5%), T. verrucosum (7,5%) e T. mentagrophytes (1,9%). N o houve predile o quanto ao sexo. A faixa etária mais acometida foi a de 0 a 10 anos, e 71,7% dos doentes s o caucasóides. CONCLUS O: Comparando os resultados obtidos com publica es anteriores da Regi o Sudeste, os autores salientam as diferen as das variantes socioecon micas na epidemiologia da doen a.
The potential of Origanum vulgare L. (Lamiaceae) essential oil in inhibiting the growth of some food-related Aspergillus species
Carmo, Egberto Santos;Lima, Edeltrudes de Oliveira;Souza, Evandro Leite de;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000200030
Abstract: origanum vulgare l. (lamiaceae) has been currently known for their interesting antimicrobial activity being regarded as alternative antimicrobial for use is food conservation systems. this study aimed to evaluate the effectiveness of o. vulgare essential oil in inhibiting the growth of some food-related aspergillus species (a. flavus, a. parasiticus, a. terreus, a. ochraceus, a. fumigatus and a. niger). the essential oil revealed a strong anti-aspergillus property providing an inhibition of all assayed mould strains. mic values were between 80 and 20 μl/ml being found a mic50 of 40 μl/ml. the essential oil at concentration of 80 and 40 μl/ml provided a fungicidal effect on a. flavus, a. fumigatus and a. niger noted by a total inhibition of the radial mycelial growth along 14 days of interaction. in addition, the essential oil was able to inhibit the mould spores germination when assayed at concentrations of 80 and 40 μl/ml. our results showed the interesting anti-aspergillus activity of o. vulgare essential oil supporting their possible use as anti-mould compound in food conservation.
Sensitivity of spoiling and pathogen food-related bacteria to Origanum vulgare L. (Lamiaceae) essential oil
Souza, Evandro Leite de;Stamford, Tania Lúcia Montenegro;Lima, Edeltrudes de Oliveira;
Brazilian Journal of Microbiology , 2006, DOI: 10.1590/S1517-83822006000400023
Abstract: origanum vulgare l. (oregano), lamiaceae, has been known as plant specie with prominent biological properties for a long time. this study aimed to evaluate the antibacterial activity of origanum vulgare essential oil on various gram-positive and gram-negative spoiling and/or pathogen food-related bacteria, as well as to observe its antimicrobial effectiveness in a food conservation micromodel. the results showed a strong antibacterial activity of the assayed essential oil noted by large growth inhibition zones (30-37 mm). mic values were between 20-40 μl/ml for the most bacteria strains. essential oil was able to cause significant (p<0.05) inhibitory effect on the bacteria viability providing a bacteriostatic effect after 24hours of exposure. in addition, the mic provided a significant (p<0.05) decrease of the autochthonous bacterial flora in ground meat samples stored under refrigeration. these results support the possibility of using origanum vulgare essential oil as alternative antimicrobial compound in food conservation systems.
Inhibitory effect of the essential oil from Cinnamomum zeylanicum Blume leaves on some food-related bacteria
Trajano, Vinicius Nogueira;Lima, Edeltrudes de Oliveira;Travassos, Ant?nio Eustáquio;Souza, Evandro Leite de;
Ciência e Tecnologia de Alimentos , 2010, DOI: 10.1590/S0101-20612010000300032
Abstract: cinnamomum zeylanicum blume, lauraceae, has long been known for having many biological properties. this study aimed to identify the constituents of the essential oil from c. zeylanicum leaves using gc-ms and to assess its inhibitory effect on salmonella enterica, escherichia coli, staphylococcus aureus, and pseudomonas aeruginosa based on mic and mbc determination and kill-time study. eugenol (73.27%) was the most prevalent compound in the essential oil followed by trans-β-cariophyllene (5.38%), linalool (3.31%), and alcohol cinamic acetate (2.53%). the results showed an interesting antibacterial activity of the oil with mic ranging from 1.25 to 10 μl.ml-1. mbc values were in the range of 20 - 80 μl.ml-1. a concentration of 10 and 40 μl.ml-1 of the essential oil caused a fast and steady decrease in viable cell count (2 to 5 log cycles) of all assayed strains along 24 hours. a concentration of 40 μl.ml-1 of the oil provided a total elimination of the initial inocula of s. aureus after 2 hours. these results show the possibility of regarding the essential oil from c. zeylanicum leaves as alternative sources of antimicrobial compounds to be applied in food conservation systems.
Profile of susceptibility in vitro of Trichosporon asahii and Trichosporon inkin strains against cyclic imides
Pontes, Zélia Braz Vieira da Silva;Lima, Edeltrudes de Oliveira;Cechinel Filho, Valdir;
Revista Brasileira de Ciências Farmacêuticas , 2007, DOI: 10.1590/S1516-93322007000200014
Abstract: in vitro susceptibility testing of trichosporon asahii (13 strains) and t. inkin (13 strains) against cyclic imides (succinimides, naphtalimides and maleimides) in concentrations of 200 to 6,25 μg/ml was performed according to the diffusion agar method. by the results obtained, the antifungal activity of the cyclic imides: 3,4-dichloro-n-phenyl-maleimide; 3,4-dichloro-n-phenyl-ethyl-maleimide and 3,4-dichloro-n-phenyl-propyl-maleimide (50 μg/ml) over t. asahii and t. inkin was important and it may be helpful in showing perspectives in a search for new antimicotic products.
Antibacterial activity of the essential oil of Origanum vulgare L. (Lamiaceae) against bacterial multiresistant strains isolated from nosocomial patients
Costa, Adalberto Coelho da;Santos, Bernadete Helena Cavalcanti dos;Santos Filho, Lauro;Lima, Edeltrudes de Oliveira;
Revista Brasileira de Farmacognosia , 2009, DOI: 10.1590/S0102-695X2009000200010
Abstract: antibiotics are considered the main therapeutic option to treat bacterial infections; however, there is the disadvantage of increasing bacterial resistance. thus, the research of antimicrobials of plant origin has been an important alternative. this work aimed at determining the in vitro antibacterial activity of the essential oil of origanum vulgare l. (lamiaceae) on multiresistant bacteria isolated from biological materials. 24 strains of nosocomial bacteria were used and divided into six different species that were inhibited by the essential oil in the preliminary "screening" which was accomplished by the diffusion technique in agar. mic was determined by the microdilution method, beginning with solutions with the final concentrations: 8 up to 0.125% with the following results: the four samples (100%) of escherichia coli, enterococcus faecalis and mrsa were inhibited by the essential oil at the concentration of 0.125%. three samples (75%) of acinetobacter baumannii at 0.125% and a sample (25%) at 0.5%; klebsiella pneumoniae (75%) at 0.125% and 25% at 0.25%; pseudomonas aeruginosa (75%) at 0.5% and 25% at 0.25%. mic varied from 78 to 83%. it was concluded through the obtained data that there was not difference in the minimum bactericidal concentration (0.5%) of the referred oil for gram positive as well for gram negative microorganisms.
Inhibitory action of some essential oils and phytochemicals on the growth of various moulds isolated from foods
Souza, Evandro Leite de;Lima, Edeltrudes de Oliveira;Freire, Kristerson Reinaldo de Luna;Sousa, Cristina Paiva de;
Brazilian Archives of Biology and Technology , 2005, DOI: 10.1590/S1516-89132005000200011
Abstract: the aim of this study was to determine the sensitivity profile of mould strains isolated from foods to some essential oils and phytochemicals. the assayed mould strains were: fusarium spp., rhizopus spp., aspergillus flavus, a. niger and penicillium spp. according to results, lippia alba n.e. brown, peumus boldus molina, lippia microphylla phil., citrus limon risso and cymbopogon citratus stapf. essential oil and the phytochemicals citral, eugenol and mircene showed prominent antimould activity. among the products that evidenced antimould activity, citral and eugenol showed the lowest minimum inhibitory concentrations, which was 1% and 4%, respectively, for the most of the tested mould strains.
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