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Search Results: 1 - 10 of 43952 matches for " Duojiao Wu "
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Cancer bioinformatics: A new approach to systems clinical medicine
Duojiao Wu, Catherine M Rice, Xiangdong Wang
BMC Bioinformatics , 2012, DOI: 10.1186/1471-2105-13-71
Telocytes in the urinary system
Zheng Yonghua,Zhu Tongyu,Lin Miao,Wu Duojiao
Journal of Translational Medicine , 2012, DOI: 10.1186/1479-5876-10-188
Abstract: Background Telocytes, a new type of interstitial cells, have been identified in many organs in mammals. The present studies aimed at investigating the ultrastructure, distribution and interactions of telocytes with surrounding cells in the urinary system of rats, to confirm the existence of telocytes in kidneys, ureter and urinary bladder. Methods Samples of kidney, ureter, or urinary bladder were harvested for the ultrastructure by the electron microscope. The primary culture of telocytes was performed to investigate the dynamic alterations. Results Telocytes mainly located in the sub-capsular space of kidney, or between smooth muscle bundles and in the lamina propria of ureter and urinary bladder. Telocytes established numerous contacts with macrophages in the sub-capsular space of kidney, or with smooth muscle cells, nerve endings as well as blood capillaries in the ureter and urinary bladder. The complete morphology of telocytes with telopodes was observed clearly through the primary cell culture from the kidney tissues of rats. Conclusions Our data evidenced the existence of telocytes in the urinary system, which may contribute to the tissue reparation and regeneration.
Hematopoietic stem cell transplantation induces immunologic tolerance in renal transplant patients via modulation of inflammatory and repair processes
Wu Duojiao,Qi Guisheng,Wang Xuanchuan,Xu Ming
Journal of Translational Medicine , 2012, DOI: 10.1186/1479-5876-10-182
Abstract: Background Inducing donor-specific tolerance in renal transplant patients could potentially prevent allograft rejection and calcineurin inhibitor nephrotoxicity. Combined kidney and hematopoietic stem cell transplant from an HLA-matched donor is an exploratory and promising therapy to induce immune tolerance. Investigtion of molecular mechanisms involved in the disease is needed to understand the potential process of cell therapy and develop strategies to prevent this immunologic rejection. Methods We enrolled nine patients in a clinical study in which cryopreserved donor hematopoietic stem cells were infused on days 2, 4, and 6 after kidney transplantation. One month post-transplant, 4 plasma samples were collected from combined transplants (C + Tx), and 8 plasma samples from patients with kidney transplantation alone (Tx). High abundance proteins in plasma were depleted and the two-dimensional liquid chromatography-tandem mass spectrometry coupled with iTRAQ labeling was utilized to identify the protein profiling between the two groups. Clusters of up- and down-regulated protein profiles were submitted to MetaCore for the construction of transcriptional factors and regulation networks. Results and Discussion Among the 179 identified proteins, 65 proteins were found in C + Tx with at least a 2-fold change as compared with Tx. A subset of proteins related to the complement and coagulation cascade, including complement C3a,complement C5a, precrusors to fibrinogen alpha and beta chains,was significantly downregulated in C + Tx. Meanwhile, Apolipoprotein-A1(ApoA1), ApoC1, ApoA2, ApoE, and ApoB were significantly lower in Tx compared to C + Tx. Gene ontology analysis showed that the dominant processes of differentially expressed proteins were associated with the inflammatory response and positive regulation of plasma lipoprotein particle remodeling. Conclusions Thus, our study provides new insight into the molecular events in the hematopoietic stem cell-induced immunologic tolerance.
Hydrogen Sulfide Inhibits the Development of Atherosclerosis with Suppressing CX3CR1 and CX3CL1 Expression
Huili Zhang, Changfa Guo, Duojiao Wu, Alian Zhang, Ting Gu, Liansheng Wang, Changqian Wang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041147
Abstract: Hydrogen sulfide, as a novel gaseous mediator, has been suggested to play a key role in atherogenesis. However, the precise mechanisms by which H2S affects atherosclerosis remain unclear. Therefore, the present study aimed to investigate the potential role of H2S in atherosclerosis and the underlying mechanism with respect to chemokines (CCL2, CCL5 and CX3CL1) and chemokine receptors (CCR2, CCR5, and CX3CR1) in macrophages. Mouse macrophage cell line RAW 264.7 or mouse peritoneal macrophages were pre-incubated with saline or NaHS (50 μM, 100 μM, 200 μM), an H2S donor, and then stimulated with interferon-γ (IFN-γ) or lipopolysaccharide (LPS). It was found that NaHS dose-dependently inhibited IFN-γ or LPS-induced CX3CR1 and CX3CL1 expression, as well as CX3CR1-mediated chemotaxis in macrophages. Overexpression of cystathionine γ-lyase (CSE), an enzyme that catalyzes H2S biosynthesis resulted in a significant reduction in CX3CR1 and CX3CL1 expression as well as CX3CR1-mediated chemotaxis in stimulated macrophages. The inhibitory effect of H2S on CX3CR1 and CX3CL1 expression was mediated by modulation of proliferators-activated receptor-γ (PPAR-γ) and NF-κB pathway. Furthermore, male apoE?/? mice were fed a high-fat diet and then randomly given NaHS (1 mg/kg, i.p., daily) or DL-propargylglycine (PAG, 10 mg/kg, i.p., daily). NaHS significantly inhibited aortic CX3CR1 and CX3CL1 expression and impeded aortic plaque development. NaHS had a better anti-atherogenic benefit when it was applied at the early stage of atherosclerosis. However, inhibition of H2S formation by PAG increased aortic CX3CR1 and CX3CL1 expression and exacerbated the extent of atherosclerosis. In addition, H2S had minimal effect on the expression of CCL2, CCL5, CCR2 and CCR5 in vitro and in vivo. In conclusion, these data indicate that H2S hampers the progression of atherosclerosis in fat-fed apoE?/? mice and downregulates CX3CR1 and CX3CL1 expression on macrophages and in lesion plaques.
Translational medicine as a permanent glue and force of clinical medicine and public health: perspectives (1) from 2012 Sino-American symposium on clinical and translational medicine
Jiebai Zhou, Duojiao Wu, Xinqing Liu, Shuoqi Yuan, Xiaoqiu Yang, Xiangdong Wang
Clinical and Translational Medicine , 2012, DOI: 10.1186/2001-1326-1-21
Abstract: Clinical and translational medicine (CTM) has been highly emphasized since Dr. Elias Zerhouni, the former director of the National Institutes of Health, proposed “The NIH Roadmap” in 2003 [1]. CTM is an emerging area comprising multidisciplinary research from basic sciences to medical applications and entails a close collaboration between clinicians and basic scientists across institutes. It is further defined as a two-way road: bench-to-bedside and bedside-to-bench [2], to translate discoveries from the bench into clinical application and/or the translation of clinical findings into the understanding of molecular mechanisms. CTM was emphasized to play a unique and critical role in optimizing new biotechnologies, improving clinical application of new therapeutic concepts, and ultimately improving the quality of life for patients [3]. The emergence of translational science highlights the unifying framework that bridges the continuum of knowledge creation and deployment, converting fundamental discoveries to human application, advancing the information into clinical practice, disseminating best clinical practices into communities and, ultimately, modifying the behavior of populations to improve global health [4].It is of great significance to promote CTM among clinicians, basic researchers, biotechnologists, politicians, ethicists, sociologists, or investors and coordinate these efforts among different countries [5]. CTM as an inter-disciplinary science is developing widely and become a global priority. The 2012 Sino-American Symposium on Clinical and Translational Medicine (SAS-CTM) as one of milestone conferences on CTM was organized by Chinese Academy of Engineering, Chinese Academy of Medical Sciences, The U.S. National Institutes of Health Clinical Center, and GlobalMD Organization. The SAS-CTM was established as a bridge between China and the U.S. to exchange ideas on clinical and translational research, built up the highest level and most influential collaborat
Clinical data integration of distributed data sources using Health Level Seven (HL7) v3-RIM mapping
Teeradache Viangteeravat, Matthew N Anyanwu, Venkateswara Nagisetty, Emin Kuscu, Mark Sakauye, Duojiao Wu
Journal of Clinical Bioinformatics , 2011, DOI: 10.1186/2043-9113-1-32
Abstract: We designed and developed a prototype implementation of HL7 v3-RIM mapping function to integrate distributed clinical data sources using R-MIM classes from HL7 v3-RIM as a global view along with a collaborative centralized web-based mapping tool to tackle the evolution of both global and local schemas. Our prototype was implemented and integrated with a Clinical Database management Systems CDMS as a plug-in module. We tested the prototype system with some use case scenarios for distributed clinical data sources across several legacy CDMS. The results have been effective in improving information delivery, completing tasks that would have been otherwise difficult to accomplish, and reducing the time required to finish tasks which are used in collaborative information retrieval and sharing with other systems.We created a prototype implementation of HL7 v3-RIM mapping for information integration between distributed clinical data sources to promote collaborative healthcare and translational research. The prototype has effectively and efficiently ensured the accuracy of the information and knowledge extractions for systems that have been integratedHealth information integration (HII) is one of the crucial challenges for healthcare systems to integrate heterogeneous data sources into a standard format. This process allows researchers to extract information and knowledge from the integrated systems effectively and efficiently using information retrieval (IR) methods, such as semantic similarity mapping [1]. Many practices realize that performing integration tasks in legacy health information systems is a cumbersome one due to the restrictions in semantic interoperability requirements. The lack of standards is a barrier for the electronic health record (EHR) implementation and integrated delivery systems which support health information exchange (HIE) in disparate health information management systems (HIMS) [2]. In order to integrate the discussed standards, a unified metho
JAK2-Centered Interactome Hotspot Identified by an Integrative Network Algorithm in Acute Stanford Type A Aortic Dissection
Sun Pan, Duojiao Wu, Andrew E. Teschendorff, Tao Hong, Linyan Wang, Mengjia Qian, Chunsheng Wang, Xiangdong Wang
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0089406
Abstract: The precise mechanisms underlying dissections, especially those without connective tissue diseases or congenital vascular diseases, are incompletely understood. This study attempted to identify both the expression profile of the dissected ascending aorta and the interactome hotspots associated with the disease, using microarray technology and gene regulatory network analysis. There were 2,737 genes differentially expressed between patients with acute Stanford type A aortic dissection and controls. Eight interactome hotspots significantly associated with aortic dissection were identified by an integrative network algorithm. In particular, we identified a JAK2-centered expression module, which was validated in an independent gene expression microarray data set, and which was characterized by over-expressed cytokines and receptors in acute aortic dissection cases, indicating that JAK2 may play a key role in the inflammatory process, which potentially contributes to the occurrence of acute aortic dissection. Overall, the analytical strategy used in this study offered the possibility to identify functional relevant network modules and subsequently facilitated the biological interpretation in the complicated disease.
Development and promotion in translational medicine: perspectives from 2012 sino-american symposium on clinical and translational medicine
Mengjia Qian, Duojiao Wu, Ena Wang, Francesco M Marincola, Wei Wang, William Rhodes, Michael Liebman, Chunxue Bai, Ching-Wan Lam, Gyorgy Marko-Varga, Thomas E Fehniger, Roland Andersson, Xiangdong Wang
Clinical and Translational Medicine , 2012, DOI: 10.1186/2001-1326-1-25
Abstract: This Session focused discussing on new models for project development and promotion in translational medicine. The conference stimulated the scientific and commercial communication of project development between academies and companies, shared the advanced knowledge and expertise of clinical applications, and created the environment for collaborations.Although strategic collaborations between corporate and academic institutions have resulted in a state of resurgence in the market, new cooperation models still need time to tell whether they will improve the translational medicine process.Clinical translational medicine (CTM) is an emerging area comprising multidisciplinary research from basic science to medical applications and entails a close collaboration among hospital, academia and industry [1]. CTM is to bridge the divide between health informatics ‘bench research’ and the application of informatics in clinical and health care settings [2]. The critical care community is beginning to adopt an increasingly translational approach to research, drug development and early-phase clinical trials [3].The 2012 Sino-American Symposium on Clinical and Translational Medicine (SAS-CTM) served as one of the milestone conferences on CTM and was organized by Chinese Academy of Engineering, Chinese Academy of Medical Sciences, The U.S. National Institutes of Health Clinical Center, and Global MD Organization. As a part of 2012 SAS-CTM, session seven, Project Development and Promotion in Translational Medicine, was co-chaired by Dr. Roland Anderson, Professor of Department of Surgery, Clinical Sciences,Medical Faculty, Lund University and Dr. Xiangdong Wang, Professor of Medicine and Director, Biomedical Research Center, Fudan University Zhongshan Hospital. The Session focused on new models for project development and promotion in translational medicine.Translational medicine is a growing and emerging area that integrates basic, social, clinical and political science together to
Therapeutic Effect of Kaixin Jieyu Decoction on the Behavior and Cerebral Blood Flow of Bilateral Carotid Artery-Ligated in Rats

Wu Wei,Wang Yanyun,Huang Shijing,Zheng Jun,Li Duojiao,Pan Juhu,Zhang Xianhui,Cun Hanming,Wang Yulei,
吴 巍
,王彦云,黄世敬,郑 军,李多娇,潘菊华,张先慧,崔翰明,王玉磊

世界科学技术-中医药现代化 , 2010,
Abstract: This work aimed to investigate the effect of Kaixin Jieyu Decoction on the behavior and cerebral blood flows of permanent bilateral carotid artery-ligated rats. The bilateral carotid artery-ligated rats were used as the animal models. By laser Doppler flowmetry detection, the rat frontal and parietal cerebral blood flow (CBF) were determined. The rat memory function was detected by the Morris water maze. Three days after administration of Kaixin Jieyu, the rat frontal and parietal CBF, as well as memory, of the experimental group were improved, but with no statistic significance. The results show that Kaixin Jieyu Decoction has some therapeutic effect on rat permanent cerebral ischemia caused by bilateral carotid artery ligation, but further investigation is needed.
A QoS-aware Method for Web Services Discovery  [PDF]
Bian WU, Xincai WU
Journal of Geographic Information System (JGIS) , 2010, DOI: 10.4236/jgis.2010.21008
Abstract: The non-functional QoS (quality of service) information helps us to select a proper Web-service from the web applications, by using component services such as UDDI[1](Universal Description, Discovery, and Integration) and MDS(Monitoring and Discovery System). MDS is a suite of web services to monitor and discover resources and service on Grids, but MDS only based on function aspects. This paper studies on an approach to provide the QoS information and a discovery model by using MDS and gives a system deployment and implementation plan. The simulation results show that the method is effective in service discovery.
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