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Theconstruction of an integrative shuttle expression
vector and potential utility was reported inEscherichiacoliandAnabaena(Nostoc) sp. strain PCC 7120. The vector comprised of the
following elements: (a) an intergenic non-coding region fromAnabaenato
facilitate its genomic integration (b) a strong functional PpsbAIpromoter fromAnabaenafor desired gene expression
and (c) neomycin phosphotransferase gene with its own promoter for the selection
The constructed vectorpAnFP was evaluated by cloning, transfer
and expression of thegfpgene encoding green fluorescent protein. When theE.coliandAnabaenasp.
strain PCC 7120 were transformed, intensive green fluorescence produced by the products
of GFP protein was observed. This
result indicated that the integrative shuttle vector pAnFP can be promisingly used in genome transformation
for expression of heterologous genes inE.coliand microalgae such
Multichannel synthetic aperture radar (SAR)
in azimuth can resolve the contradiction between high resolution and wide swath
faced with traditional SAR imaging. However, channel errors will degrade the
performance of imaging. This paper compares the performances of four channel error
estimation algorithms under different clutter distributions and SNR conditions.
Further, explanations are given for performance differences of the four
algorithms, which provide evidence for method selection in engineering applications.