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Search Results: 1 - 10 of 194267 matches for " Divina das Dores de Paula;Martins "
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Seroprevalence of hepatitis B virus infection and seroconvertion to anti-HBsAg in laboratory staff in Goiania, Goiás
Silva, Paula Andréia;Fiaccadori, Fabíola Souza;Borges, Ana Maria Tavares;Silva, Simone Almeida;Daher, Roberto Ruhman;Martins, Regina Maria Bringel;Cardoso, Divina das Dores de Paula;
Revista da Sociedade Brasileira de Medicina Tropical , 2005, DOI: 10.1590/S0037-86822005000200005
Abstract: were analyzed 648 serum samples from laboratory staff in goiania, goiás aiming detection of three serological markers of hbv: hbsag, anti-hbsag and anti-hbcag. the hbsag and anti-hbcag positive samples were also analyzed for hbeag, anti-hbeag and anti-hbcagigm markers. hbv infection rate of 24.1% was observed and, from them, 0.7% were positive for hbsag. viral dna was detected by pcr in two hbsag positive samples. a vaccination index of 74.5% and a global index of 89.9% of serological response to vaccination were observed. the direct work with biological fluids as well as cleaning workers represented significant risks for acquisition of hbv infection. the data from the present study showed an increase of the vaccination index among laboratory staff but the rates of hbv infection did not change through the years in the region.
Rotavirus e adenovirus em crian?as de 0-5 anos hospitalizadas com ou sem gastrenterite em Goiania - GO., Brasil
Cardoso, Divina das Dores de Paula;Martins, Regina Maria Bringel;Kitajima, Eliott W.;Barbosa, Aristides José;Camarota, Sandra Cristina Teles;Azevedo, Maril Silva Pereira;
Revista do Instituto de Medicina Tropical de S?o Paulo , 1992, DOI: 10.1590/S0036-46651992000500010
Abstract: in order to detect rotavirus and adenovirus 557 feces samples from hospitalized children (0-5 years of age) were analysed from june 1987 to july 1990 in goiania-city.two hundred and ninety one samples were from children with diarrhoea and 266 were from children without diarrhoea. amongst this later group, 64 samples were from children from the nursery. two hundred and sixty one out of 557 samples were analysed by immunoelectron microscopy (iem), polyacrylamide gel electrophoresis (sds-page) and enzymatic immunoassay for rotavirus and adenovirus (eiara) whereas the rest (296 samples) were analysed by sds-page and eiara. positivity of rotavirus and adenovirus was 17.2% and 2.1% respectively. concerning rotavirus and adenovirus there was 29.2% and 2.4% positivity within the group with diarrhoea and 4.1% and 1.5% positivity amongst children without diarrhoea (p<0.05). rotavirus were more prevalent amongst children which age ranged from 1 to 11 months of age. no newborn child from the nursery was positive for rotavirus. adenovirus were detected amongst children from 1 to 3 years of age. rotavirus circulation peak occurred between may and august (p<0.05) and no positive case was detected from december to february. two hundred out of 291 diarrheic samples were also studied concerning bacteria and pathogenic parasites and equal percentages (17.0%) were found for both rotavirus and pathogenic bacteria. eighty nine samples of rotavirus were detected by sds-page and 86 of these (96.6%) belonged to the subgroup ii with 13 different electrophoretic patterns. predominance of a given eletropherotic profile was observed in each year of the study.
Monitoring the circulation of rotavirus among children after the introduction of the RotarixTM vaccine in Goiania, Brazil
Borges, Ana Maria Tavares;Souza, Menira Dias e;Fiaccadori, Fabíola Souza;Cardoso, Divina das Dores de Paula;
Memórias do Instituto Oswaldo Cruz , 2011, DOI: 10.1590/S0074-02762011000400018
Abstract: the epidemiological features of rotavirus a (rva) infection differ between children from developing and developed countries which could result in differences in vaccine efficacy around the world. to evaluate the impact of rotarixtm on rva prevalence, we monitored rva genotypes circulating in goiania by monitoring virus in faecal samples from children that had or had not been previously vaccinated. from february-november of 2008, 220 faecal samples were collected from children in seven day-care centres. rva detection was performed by two methodologies and the results were confirmed by polyacrylamide gel electrophoresis. from the 220 samples, eight were rva-positive (3.6%) and five were from children that had received either one or two doses of the vaccine. all positive samples were collected from children with diarrhoea during august and september. genotyping of the rva characterised five of the viral samples as genotype g2p[4] and one as g8p[4], suggesting that g2p[4] was the predominant circulating genotype in goiania during the study. the fact that vaccinated children were also infected by rva suggests that the vaccine does not fully protect against infection by the g2[p4] rva genotype.
Serological markers and risk factors related to hepatitis B virus in dentists in the Central West region of Brazil
Paiva, Enilza Maria Mendon?a de;Tiplle, Anaclara Ferreira Veiga;Silva, Eliane de Paiva e;Cardoso, Divina das Dores de Paula;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000200010
Abstract: the hepatitis b virus (hbv) has been considered the major occupational risk agent for dentists. the central west region of brazil is considered an intermediate endemic pattern area, but currently there is no information about the hbv prevalence in dentists of goiania, goiás. this study aimed at the detection of the hbv infection rate and risk factors for dentists of goiania and the comparison of the obtained data with the general population and other groups. a randomized sample of 680 professionals participated in this study. all dentists gave written consent for the procedure and filled out a questionnaire about risk factors. the hbv serological markers were analyzed using elisa test and the presence of anti-hbc was observed in 41 (6.0%) of the dentists. none of them was hbsag positive. significant relationships with hbv positivity were observed with gender, the time working as a dentist and the use of incomplete personal protective equipment (ppe). the hbv prevalence found in this group of dentists was lower than the endemic pattern of the general population, other health care workers of the region and the dentists from other regions in brazil. these results may indicate a positive impact of vaccination considering the high adherence of the dentists to the immunization program (98.4%). finally, the use of complete ppe by the majority as well as other standard precautions recommended for health care workers could be responsible for the low hbv seroprevalence.
Considera??es metodológicas na interpreta??o do rastreamento sorológico da hepatite B em doadores de sangue
Martelli,Celina Maria Turchi; Andrade,Ana Lúcia Sampaio Sgambatti de; Cardoso,Divina das Dores Paula; Silva,Simonne Almeida e; Zicker,Fabio;
Revista de Saúde Pública , 1991, DOI: 10.1590/S0034-89101991000100003
Abstract: between october 1988 and february 1989, 1,033 voluntary first-time blood donors were screened for hepatitis b infection in five blood banks in goiania, central brazil. the survey was part of a major study designed to estimate seroprevalence of hbsag and anti-hbs and to discuss methodological issues related to prevalence estimation based on data from blood banks. donors were interviewed and blood samples were collected and tested for hbsag and anti-hbs by elisa tests. prevalences of 1.9% and 10.9% were obtained for hbsag and anti-hbs, respectively, and no statistical difference was found between the sexes. prevalence of anti-hbs increased with age (x2 for trend = 7.9 p = 0.004). the positive predictive value and sensitivity of history of jaundice or hepatitis reported in the interview in detecting seropositives were 13.6% and 2.2%, respectively. the methodological issues, including internal and external validity of hbv prevalence estimated among blood donors are discussed. the potential usefulness of blood banks as a source of morbidity information for surveillance for hepatitis b virus infection is stressed.
Soroprevalência e fatores de risco para a infec??o pelo vírus da hepatite B pelos marcadores AgHBs e anti-HBs em prisioneiros e primodoadores de sangue
Martelli,Celina Maria Turchi; Andrade,Ana Lúcia Sampaio Sgambatti de; Cardoso,Divina das Dores Paula; Sousa,Luiz Carlos Silva; Silva,Simonne Almeida e; Sousa,Marta Antunes de; Zicker,Fábio;
Revista de Saúde Pública , 1990, DOI: 10.1590/S0034-89101990000400004
Abstract: two cross-sectional surveys on hepatitis b virus (hbv) infection were carried out among 1,033 volunteer first-time blood donors in five blood banks (3 private, 2 public) and among 201 prisoners in the penitentiary center of industrial activity, in goiania, central brazil, between june 1988 and february 1989. those surveys were part of a major study designed to estimate seroprevalence of hbsag and anti-hbsag markers by elisa test, and to study risk factors associated with seropositivity. the presence of any serum marker was considered as previous exposure to hbv. a standard questionnaire was applied to both populations to evaluate previous blood transfusion, number of sexual partners, homo/bisexual activity, history of sexually transmitted diseases, drug abusers, use of parenteral medicine, accupunture, tattooing and vdrl seropositivity. seroprevalence varied from 12.8% to 26.4% in blood donors and prisoners, respectively, (p<0.05) and increased with age (x2 trend=14.0 p<0.05%). prisoners had higher percentages of all risk factors investigated than blood donors, with the exception of number of sexual partners. among all risk factors studied, age, imprisonment and tattooing were statistically associated with seropositivity, even after multivariate analysis controlling for age and reclusión. the paper discusses the methodologic issues related to this epidemiologic investigation.
Adenovirus, calicivirus and astrovirus detection in fecal samples of hospitalized children with acute gastroenteritis from Campo Grande, MS, Brazil
Andreasi, Marcia Sueli Assis;Cardoso, Divina das Dores de Paula;Fernandes, Sonia Maria;Tozetti, Ines Aparecida;Borges, Ana Maria Tavares;Fiaccadori, Fabíola Souza;Santos, Rodrigo Alessandro T?go;Souza, Menira;
Memórias do Instituto Oswaldo Cruz , 2008, DOI: 10.1590/S0074-02762008000700020
Abstract: we analyzed fecal samples from hospitalized children up to three years of age with acute gastroenteritis at campo grande, mato grosso do sul, brazil, from may 2000-january 2004. astrovirus and calicivirus were detected by reverse transcription-polymerase chain reaction and adenovirus was detected using the rotavirus and adenovirus combined immunoenzyme assay. astrovirus, adenovirus and calicivirus were detected at rates of 3.1%, 3.6% and 7.6%, respectively. these results re-emphasize the need for the establishment of regional vigilance systems to evaluate the impact of enteric viruses on viral gastroenteritis.
Rotavírus A em crian?as de até três anos de idade, hospitalizadas com gastroenterite aguda em Campo Grande, Estado do Mato Grosso do Sul
Andreasi, Márcia Sueli Assis;Batista, Sonia Maria Fernandes;Tozetti, Inês Aparecida;Ozaki, Cláudia Okanobo;Nogueira, Mariana Menegusso;Fiaccadori, Fabíola Souza;Borges, Ana Maria Tavares;Santos, Rodrigo Alessandro Togo;Cardoso, Divina das Dores de Paula;
Revista da Sociedade Brasileira de Medicina Tropical , 2007, DOI: 10.1590/S0037-86822007000400008
Abstract: polyacrylamide gel electrophoresis and combined immunoenzyme assay for rotavirus and adenovirus were used to analyze 380 fecal samples from children up to three years of age who were hospitalized with acute diarrhea in campo grande, state of mato grosso do sul, between may 2000 and january 2004. among all the samples, 88 (23. 2%) were positive for rotavirus a. out of these, 81 (92%) had a defined electrophoretic pattern: 77 (87. 5%) with a long pattern and four (4. 5%) with a short pattern. genotype g and p characterization was done by nested rt-pcr for 85 samples, of which 56 (65. 9%) were genotyped as type g. among these, 49 (87. 5%) were g1, five (8. 9%) were g4, one (1. 8%) was g3 and one (1. 8%) was g9. the genotype was found to be type p in 37 samples (43. 5%) and all of these were p[8]. the g and p association most observed was g1p[8], with 33 samples (89. 2%), followed by g4p[8], two samples (5. 4%); g3p[8], one sample (2. 7%); and g9p[8], one sample (2. 7%).
Soroprevalência da infec??o pelo vírus da hepatite B em portadores de doen?a mental
Souza, Márcia Maria de;Barbosa, Maria Alves;Borges, Ana Maria Tavares;Daher, Roberto Ruhman;Martins, Regina Maria Bringel;Cardoso, Divina das D?res de Paula;
Revista Brasileira de Psiquiatria , 2004, DOI: 10.1590/S1516-44462004000100010
Abstract: objectives: hepatitis b virus (hbv) infection is an important worldwide public health problem and it has been cause of elevated morbidity and mortality rates. the objectives of this study were determine the hbv infection seroprevalence in psychiatric institutions and in individuals with down's syndrome, detect viral dna in hbsag and anti-hbc total positive serum samples and determine the hbsag subtypes circulating these groups. methods: the study assessed 433 subjects, with 408 being mentally disordered inpatients (71 had also chemical dependence), and 25 were down's syndrome outpatientes. blood samples were collected and tested for hbv markers: hbsag, anti-hbs and anti-hbc total by enzyme-linked immunosorbent assay (elisa). hbsag positive samples were also tested for anti-hbc igm, hbeag, anti-hbe, and subtyped by radial immunodifusion. hbv-dna was investigated in hbsag and/or anti-hbc total positive samples by pcr methodology. results: a global hbv positivity of 22.4% was detected. hbsag was found in 1.6% of the samples. among them, five were subtyped as adw2, adw4 and ayw3. dna viral was found in 3 hbsag samples and 11 hbsag and anti-hbc total/anti-hbs positive samples, respectively. the risk factors analysis showed that multiple hospital admission were significantly associated with hbv markers. conclusion: these results show high hbv seroprevalence in groups investigated and reinforce the importance of hbv specifics preventive measures to reduce the risk of hepatitis b in individuals with mental disturbs and retard.
Seroprevalence of hepatitis B virus infection in individuals with clinical evidence of hepatitis in Goiania, Goiás: detection of viral DNA and determination of subtypes
SILVA, Claudia de Oliveira e;AZEVEDO, Marli da Silva Pereira;SOARES, Célia Maria de Almeida;MARTINS, Regina Maria Bringel;RAMOS, Carmen Helena;DAHER, Roberto Ruhman;CARDOSO, Divina das D?res de Paula;
Revista do Instituto de Medicina Tropical de S?o Paulo , 2002, DOI: 10.1590/S0036-46652002000600007
Abstract: the presence of serological markers for hepatitis b virus (hbsag, anti-hbc igm and anti-hbc total) was investigated in the serum of 1,396 individuals who had clinical suspect of hepatitis. it was observed that 50.7% of the individuals were positive and, from the total of the studied individuals, 14.5% were positive for hbsag. from these, 8.5% were also positive for anti-hbc igm. the analysis in relation to gender showed a higher seroprevalence index among male individuals (p < 0.0001). it was observed the occurrence of subtypes adw2 (62.7%), ayw3 (23.5%), ayw2 (9.8%) and adw4 (3.9%). the viral dna was detected in 61 (33.9%) hbsag positive samples and in one sample positive only for anti-hbc total. these results indicate an important incidence of the hbv infection in this population, and reinforce previous studies regarding this virus in the central west region of brazil.
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