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Search Results: 1 - 10 of 117955 matches for " Diqiu Wang "
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Gothicism in The Fall of the House of Usher  [PDF]
Wenfang Pang, Diqiu Wang, Shanshan Hu
Advances in Literary Study (ALS) , 2015, DOI: 10.4236/als.2015.31003
Abstract: Edgar Allan Poe is one of the most unique writers in America. He stands alone with his aesthetic taste and writing principle, engaged in the morbid theme of nightmare, death, crime and evil. Consequently, he adopts Gothic technique in a composition, taking a full advantage of Gothic subject matter, plot and elements and lingering on violence, murder, insanity and collapse. The Fall of the House of Usher is Poe’s classical piece of this type. It presents terrifying atmosphere, the dark plot, and man’s psychological terror to reveal the process of disintegration and annihilation of human mind, thus offering readers specific aesthetic perception through psychological shocks. This paper borrowing a critical perspective of Gothic tradition and theory of the sublime, tries to analyze the Gothicism in this novel to detect its aesthetic feature.
Arabidopsis WRKY2 transcription factor mediates seed germination and postgermination arrest of development by abscisic acid
Wenbo Jiang, Diqiu Yu
BMC Plant Biology , 2009, DOI: 10.1186/1471-2229-9-96
Abstract: To determine directly the role of Arabidopsis WRKY2 transcription factor during ABA-dependent seed germination and postgermination growth arrest, we isolated T-DNA insertion mutants. Two independent T-DNA insertion mutants for WRKY2 were hypersensitive to ABA responses only during seed germination and postgermination early growth. wrky2 mutants displayed delayed or decreased expression of ABI5 and ABI3, but increased or prolonged expression of Em1 and Em6. wrky2 mutants and wild type showed similar levels of expression for miR159 and its target genes MYB33 and MYB101. Analysis of WRKY2 expression level in ABA-insensitive and ABA-deficient mutants abi5-1, abi3-1, aba2-3 and aba3-1 further indicated that ABA-induced WRKY2 accumulation during germination and postgermination early growth requires ABI5, ABI3, ABA2 and ABA3.ABA hypersensitivity of the wrky2 mutants during seed germination and postgermination early seedling establishment is attributable to elevated mRNA levels of ABI5, ABI3 and ABI5-induced Em1 and Em6 in the mutants. WRKY2-mediated ABA responses are independent of miR159 and its target genes MYB33 and MYB101. ABI5, ABI3, ABA2 and ABA3 are important regulators of the transcripts of WRKY2 by ABA treatment. Our results suggest that WRKY2 transcription factor mediates seed germination and postgermination developmental arrest by ABA.Abscisic acid (ABA) is a phytohormone regulating plant responses to a variety of environmental stress, particularly water deprivation, notably by regulating stomatal aperture [1-4]. It also plays an essential role in mediating the initiation and maintenance of seed dormancy [5]. Late in seed maturation, the embryo develops and enters a dormant state that is triggered by an increase in the ABA concentration. This leads to the cessation of cell division and activation of genes encoding seed storage proteins and proteins required to establish desiccation tolerance [6]. Exposure of seeds to ABA during germination leads to rapid but rev
The Use of Fourier Transform Infrared Spectroscopy for Quantification of Adulteration in Virgin Walnut Oil
Pengjuan Liang,Hao Wang,Chaoyin Chen,Feng Ge,Diqiu Liu,Shiqi Li,Benyong Han,Xianfeng Xiong,Shenglan Zhao
Journal of Spectroscopy , 2013, DOI: 10.1155/2013/305604
Abstract: Currently, the authentication of virgin walnut oil (VWO) has become very important due to the possible adulteration of VWO with cheaper plant oils such as soybean oil (SO), puer tea seed oil (PO), and sunflower oil (SFO). Methods involving Fourier transform infrared (FT-IR) spectroscopy combined with chemometric techniques (partial least square) were developed for quantification of SO, PO, and SFO in VWO. IR spectra of oil samples were recorded at frequency regions of 4000–650?cm?1 on horizontal attenuated total reflectance (HATR) attachment of FT-IR. PLS model correlates the actual and FT-IR estimated values of oil adulterants (SO, PO, and SFO) with coefficients of determination ( ) of 0.9958, 0.9925, and 0.9952, respectively. The obtained RMSEC values of SO, PO, and SFO in VWO are 1.35%, 1.85%, and 1.43% (v/v), respectively. The method, therefore, has potential as a rapid method for quantification of product adulteration. 1. Introduction In the recent years, walnut oil has received great attention because of its biological activities and sensory qualities and has become a very important agricultural product for many countries. The unsaturated fatty acid in walnut oil is 90%. There is about 47.4% of linoleic acid and 15.8% of linolenic acid [1]. Epidemiological studies show that walnut oil not only reduces serum cholesterol but at the same time has nutritional cranial nerve cells which can adjust plant nerve function. Other experts discovered that walnut oil does not only act as officinal, but also can be used as the “old man and the infant nutrition oil” as well as aerial work and flight personnel’s senior health care oil [2]. Due to its higher price in the market, WO can be a target of adulteration with the cheaper oils such as soybean oil in order to gain economical profit. Therefore, the development of rapid and nonexpensive analytical techniques capable of detecting such adulterations in walnut oil is currently highly demanded. In fact, the adulteration is a serious problem in trade of fats and oils for a long time, and it is of primary importance for consumers, food processors, and industries, because there is a great difference in quality and price for different oil products. The adulteration is increasingly more difficult to detect when the oil adulterant has similar chemical composition to the authentic oil [3, 4]. In addition, chemical methods traditionally employed for the control of authenticity of virgin edible oil as gas chromatography and high performance liquid chromatography are expensive, time-consuming, require skilled operators, and
Identification of Nitrogen Starvation-Responsive MicroRNAs in Arabidopsis thaliana
Gang Liang, Hua He, Diqiu Yu
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0048951
Abstract: microRNAs (miRNAs) are a class of negative regulators that take part in many processes such as growth and development, stress responses, and metabolism in plants. Recently, miRNAs were shown to function in plant nutrient metabolism. Moreover, several miRNAs were identified in the response to nitrogen (N) deficiency. To investigate the functions of other miRNAs in N deficiency, deep sequencing technology was used to detect the expression of small RNAs under N-sufficient and -deficient conditions. The results showed that members from the same miRNA families displayed differential expression in response to N deficiency. Upon N starvation, the expression of miR169, miR171, miR395, miR397, miR398, miR399, miR408, miR827, and miR857 was repressed, whereas those of miR160, miR780, miR826, miR842, and miR846 were induced. miR826, a newly identified N-starvation-induced miRNA, was found to target the AOP2 gene. Among these N-starvation-responsive miRNAs, several were involved in cross-talk among responses to different nutrient (N, P, S, Cu) deficiencies. miR160, miR167, and miR171 could be responsible for the development of Arabidopsis root systems under N-starvation conditions. In addition, twenty novel miRNAs were identified and nine of them were significantly responsive to N-starvation. This study represents comprehensive expression profiling of N-starvation-responsive miRNAs and advances our understanding of the regulation of N homeostasis mediated by miRNAs.
Overexpression of the stress-induced OsWRKY08 improves osmotic stress tolerance in Arabidopsis
Yu Song,ShaoJuan Jing,DiQiu Yu
Chinese Science Bulletin , 2009, DOI: 10.1007/s11434-009-0710-5
Abstract: Previous Northern blotting analyses of rice seedlings have screened several WRKY genes among the transcripts that are differentially regulated in the following treatments: high salinity, cold stress, polyethylene glycol (PEG) and heat shock. Here, we report characterization of a WRKY gene, OsWRKY08, in rice, which was found to be inducible by PEG, NaCl, Abscisic acid (ABA), and naphthalene acetic acid (NAA) as its ortholog AtWRKY28 in Arabidopsis. To determine whether overexpression of OsWRKY08 alters abiotic stress tolerance, 35S::OsWRKY08 recombinant was generated and transformed into Arabidopsis. Physiological tests indicated that 35S::OsWRKY08 transgenic Arabidopsis displayed increased tolerance to mannitol stress through increasing the lateral root number and primary root length during seeding root development. Further, semi-quantitative RT-PCR showed that AtCOR47 and AtRD21, two ABA-independent abiotic stress responded genes, were induced in 35S::OsWRKY08 transgenic plants. These results suggest OsWRKY08 improves the osmotic stress tolerance of transgenic Arabidopsis through an ABA-independent signaling pathway.
Cloning and analysis of expression profile of 13WRKY genes in rice
Yuping Qiu,Shaojuan Jing,Jian Fu,Lu Li,Diqiu Yu
Chinese Science Bulletin , 2004, DOI: 10.1007/BF03185783
Abstract: The transcriptional factor WRKY proteins contain the highly conserved amino acid sequence WRKYGQK as well as the novel zinc-finger-like motifs Cys2His2 or Cys2HisCys. A search of the rice genome identified 97 genes encoding WRKY proteins. Of these 97 WRKY homologs found in rice, 13 cDNAs encoding WRKY proteins were consequently isolated from a rice cDNA library constructed from 4°C-treated shoots by probing for the conserved WRKY domain. Northern blotting analysis revealed that 10 of 13OsWRKY genes were differentially regulated in plants that were treated by the four following abiotic stress factors: NaCl, PEG, cold (4°C) and heat (42°C). The resulting expression profiles exhibited great differences in both the manner and timing of their response to the four different abiotic treatments. The difference of gene expression profiles suggested the different physiological functions among theWRKY genes.
Elevated Levels of MYB30 in the Phloem Accelerate Flowering in Arabidopsis through the Regulation of FLOWERING LOCUS T
Liangyu Liu, Jian Zhang, Jessika Adrian, Lionel Gissot, George Coupland, Diqiu Yu, Franziska Turck
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0089799
Abstract: In Arabidopsis thaliana, the R2R3 MYB-like transcription factor MYB30 is a positive regulator of the pathogen-induced hypersensitive response and of brassinosteroid and abscisic acid signaling. Here, we show that MYB30 expressed under the control of the strong phloem-specific SUC2 promoter accelerates flowering both in long and short days. Early flowering is mediated by elevated expression of FLOWERING LOCUS T (FT), which can be observed in the absence and presence of CONSTANS (CO), the main activator of FT. CO-independent activation by high MYB30 expression results in FT levels that remain below those observed in the wild-type plants, which show an additive CO-dependent activation. In contrast, TWIN SISTER OF FT (TSF) is repressed in plants expressing high levels of MYB30 in the phloem. In transient assays, MYB30 and CO additively increase the activity of a reporter construct driven by a 1 kb FT promoter. Acceleration of flowering by MYB30 does not require the presence of salicylic acid and is independent of FLC. Taken together, increased levels of MYB30, which was reported to be induced in response to the perception of pathogens, can accelerate flowering and MYB30 may thus be a candidate to mediate cross-talk between gene networks involved in biotic stress perception and flowering time.
A GmRAV Ortholog Is Involved in Photoperiod and Sucrose Control of Flowering Time in Soybean
Qingyao Lu, Lin Zhao, Dongmei Li, Diqiu Hao, Yong Zhan, Wenbin Li
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0089145
Abstract: Photoperiod and sucrose levels play a key role in the control of flowering. GmRAV reflected a diurnal rhythm with the highest expression at 4 h after the beginning of a dark period in soybean leaves, and was highly up-regulated under short-day (SD) conditions, despite of not following a diurnal pattern under long-day (LD) conditions. GmRAV-i (GmRAV-inhibition) transgenic soybean exhibited early flowering phenotype. Two of the FT Arabidopsis homologs, GmFT2a and GmFT5a, were highly expressed in the leaves of soybeans with inhibition (-i) of GmRAV under SD conditions. Moreover, the transcript levels of the two FT homologs in GmRAV-i soybeans were more sensitive to SD conditions than LD conditions compared to the WT plant. GmRAV-i soybeans and Arabidopsis rav mutants showed more sensitive hypocotyl elongation responses when compared with wild-type seedlings, and GmRAV-ox overevpressed in tobacco revealed no sensitive changes in hypocotyl length. These indicated that GmRAV was a novel negative regulator of SD-mediated flowering and hypocotyl elongation. Although sucrose has been suggested to promote flowering induction in many plant species, high concentration of sucrose (4% [w/v]) applied into media defer flowering time in Arabidopsis wild-type and rav mutant. This delayed flowering stage might be caused by reduction of LEAFY expression. Furthermore, Arabidopsis rav mutants and GmRAV-i soybean plants were less sensitive to sucrose by the inhibition assays of hypocotyls and roots growth. In contrast, transgenic GmRAV overexpressing (-ox) tobacco plants displayed more sensitivity to sucrose. In conclusion, GmRAV was inferred to have a fundamental function in photoperiod, darkness, and sucrose signaling responses to regulate plant development and flowering induction.
Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis
LiLi Tu,XianLong Zhang,DiQiu Liu,ShuangXia Jin,JingLin Cao,LongFu Zhu,FengLin Deng,JiaFu Tan,CunBin Zhang
Chinese Science Bulletin , 2007, DOI: 10.1007/s11434-007-0461-0
Abstract: The mechanisms of cotton fiber development and somatic embryogenesis have been explored systematically with microarray and suppression subtractive hybridization. Real-time RT-PCR provides the simultaneous measurement of gene expression in many different samples, with which the data from microarray or others can be confirmed in detail. To achieve accurate and reliable gene expression results, normalization of real-time PCR data against one or several internal control genes is required, which should not fluctuate in different tissues during various stages of development. We assessed the gene expression of 7 frequently used housekeeping genes, including 18S rRNA, Histone3, UBQ7, Actin, Cyclophilin, Gbpolyubiquitin-1 and Gbpolyubiquitin-2, in a diverse set of 21 cotton samples. For fiber developmental series the expression of all housekeeping genes had the same down tendency after 17 DPA. But the expression of the AGP gene (arabinogalactan protein) that has high expression level at the later fiber development stage was up-regulated from 15 to 27 DPA. So the relative absolute quantification should be an efficient and convenient method for the fiber developmental series. The expression of nonfiber tissues series varied not so much against the fiber developmental series. And three best control genes Histone3, UBQ7 and Gbpolyubiquitin-1 have to be used in a combinated way to get better normalization.
Application of Fourier Transform Infrared Spectroscopy for the Oxidation and Peroxide Value Evaluation in Virgin Walnut Oil
Pengjuan Liang,Chaoyin Chen,Shenglan Zhao,Feng Ge,Diqiu Liu,Binqiu Liu,Qimeng Fan,Benyong Han,Xianfeng Xiong
Journal of Spectroscopy , 2013, DOI: 10.1155/2013/138728
Abstract: Recent developments in Fourier transform infrared spectroscopy-partial least squares (FTIR-PLSs) extend the application of this strategy to the field of the edible oils and fats research. In this work, FT-IR spectroscopy was used as an effective analytical tool to determine the peroxide value of virgin walnut oil (VWO) samples undergone during heating. The spectra were recorded from a film of pure oil between two disks of KBr for each sample at frequency regions of 4000–650?cm?1. Changes in the values of the frequency of most of the bands of the spectra were observed and used to build the calibration model. PLS model correlates the actual and FT-IR estimated value of peroxide value with a correlation coefficient of 0.99, and the root mean square error of the calibration (RMSEC) value is 0.4838. The methodology has potential as a fast and accurate way for the quantification of peroxide value of the edible oils. 1. Introduction The production of walnut constitutes a significant proportion of the income of farmers in many countries. In the recent years, the peroxide value of virgin walnut oil (VWO) has received great attention because of its sensory qualities and biological activities. Epidemiological studies show that VWO not only reduces serum cholesterol but also has nutritional cranial nerve cells which can adjust plant nerve function. Other experts discover that walnut oil does not only act as an officinal but also can be used as the “old man and the infant nutrition oil” as well as aerial work and flight personnel senior’s health care oil [1]. In this way, VWO production is a promising factor to the viability of ecological economy. Nevertheless, VWO can be a target of adulteration with cheaper oils or even metamorphic oils because of its higher prices in the global market. The adulteration comprises a great hazard not only for the economic development and prosperity of those communities but also for the health and safety of VWO consumers. Therefore, the development of rapid and accurate analytical techniques capable of detecting the quality of VWO is currently highly demanded. In fact, the quality of oils depends on its chemical composition that changes qualitatively and quantitatively; one of the most important indicators of performance and shelf-life is the oxidative stability of oils, that is, their resistance to the oxidation process. Previous study showed that the unsaturated fatty acid content of oils is higher; that is, the unsaturated level is higher, and the oxidation is easier [2]. Because of the unsaturated fatty acid in VWO is 90%, and
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