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Search Results: 1 - 10 of 63 matches for " Didelphis marsupialis "
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Inmunodiagnóstico en Didelphis marsupialis usando un antígeno de Paragonimus de Venezuela
Gómez Martínez,Erika; Díaz-Bello,Zoraida; Zavala-Jaspe,Reinaldo; Tulio Díaz,Marcos; Noya,Oscar; Alarcón de Noya,Belkisyole;
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: paragonimus are trematodes that normally live in the lungs of carnivorous and omnivorous mammals such as humans. an outbreak of paragonimus sp. in which didelphis marsupialis was the only wild reservoir incriminated was described in eastern venezuela. in order to have immunological tools to detect the presence of paragonimus sp. in this reservoir, a whole antigen of the adult worm of this parasite was elaborated. didelphis marsupialis were captured in the locality of aguas blancas, montes municipality, sucre state, venezuela, from which blood samples were obtained and a search for worms was performed in lungs. worms were homogenized and ultracentrifugated to obtain fspa to perform immunoassay (elisa) to detect antibodies in opossums. the electrophoresis analysis showed a pattern of 22 molecules between 6 and 82 kda; by western blot, the antigenic recognition of 8 antigenic molecules appeared,112 kda and 268 kda molecules being the most strongly recognized by positive sera. the negative sera did not recognize any band. the production of igy in chicken enabled the development of reagents capable of performing a standard immunodiagnosis technique to find specific anti-paragonimus sp. in didelphis marsupialis in order to establish epidemiological surveillance of these reservoirs in endemic areas.
Histopathological study of experimental and natural infections by Trypanosoma cruzi in Didelphis marsupialis
Carreira, Jo?o Carlos Araujo;Jansen, Ana Maria;Deane, Maria P;Lenzi, Henrique Leonel;
Memórias do Instituto Oswaldo Cruz , 1996, DOI: 10.1590/S0074-02761996000500012
Abstract: didelphis marsupialis, the most important sylvatic reservoir of trypanosoma cruzi, can also maintain in their anal scent glands the multiplicative forms only described in the intestinal tract of triatomine bugs. a study of 21 experimentally and 10 naturally infected opossums with t. cruzi was undertaken in order to establish the histopathological pattern under different conditions. our results showed that the inflammation was predominantly lymphomacrophagic and more severe in the naturally infected animals but never as intense as those described in chagas' disease or in other animal models. the parasitism in both groups was always mild with very scarce amastigote nests in the tissues. in the experimentally infected animals, the inflammation was directly related to the presence of amastigotes nests. four 24 days-old animals, still in embryonic stage, showed multiple amastigotes nests and moderate inflammatory reactions, but even so they survived longer and presented less severe lesions than experimentally infected adult mice. parasites were found in smooth, cardiac and/or predominantly striated muscles, as well as in nerve cells. differing from the experimentally infected opossums parasitism in the naturally infected animals predominated in the heart, esophagus and stomach. parasitism of the scent glands did not affect the histopathological pattern observed in extraglandular tissues.
Humoral Immune Response Kinetics in Philander opossum and Didelphis marsupialis Infected and Immunized by Trypanosoma cruzi Employing an Immunofluorescence Antibody Test
Legey, Ana Paula;Pinho, Ana Paula S;Xavier, Samanta C Chagas;Leon, Leonor L;Jansen, Ana Maria;
Memórias do Instituto Oswaldo Cruz , 1999, DOI: 10.1590/S0074-02761999000300016
Abstract: philander opossum and didelphis marsupialis considered the most ancient mammals and an evolutionary success, maintain parasitism by trypanosoma cruzi without developing any apparent disease or important tissue lesion. in order to elucidate this well-balanced interaction, we decided to compare the humoral immune response kinetics of the two didelphids naturally and experimentally infected with t. cruzi and immunized by different schedules of parasite antigens, employing an indirect fluorescence antibody test (ifat). both didelphids responded with high serological titers to different immunization routes, while the earliest response occurred with the intradermic route. serological titers of naturally infected p. opossum showed a significant individual variation, while those of d. marsupialis remained stable during the entire follow-up period. the serological titers of the experimentally infected animals varied according to the inoculated strain. our data suggest that (1) ifat was sensitive for follow-up of p. opossum in natural and experimental t. cruzi infections; (2) both p. opossum and d. marsupialis are able to mount an efficient humoral immune response as compared to placental mammals; (3) experimentally infected p. opossum and d. marsupialis present distinct patterns of infection, depending on the subpopulation of t. cruzi, (4) the differences observed in the humoral immune responses between p. opossum and d. marsupialis, probably, reflect distinct strategies selected by these animals during their coevolution with t. cruzi.
Modification and adaptation in semi-defined media for cultivation of flagellate Tetratrichomonas didelphidis (Trichomonadidae trichomonadinae) from the Didelphis marsupialis
TASCA,TIANA; DE CARLI,GERALDO A; STEINDEL,MARIO; MATTIA,ROSSANA; ROSSET,IVELI;
Parasitología al día , 1999, DOI: 10.4067/S0716-07201999000300010
Abstract: tetratrichomonas didelphidis (hegner and ratcliffe, 1927) andersen and reilly, 1965 is a flagellate protozoan from the intestine, cecum, and colon of didelphis marsupialis linnaeus, 1758. the parasite was found and isolated in the rectal glands in pavlova starch-containing media in florianópolis, sc, brasil from d.
Modification and adaptation in semi-defined media for cultivation of flagellate Tetratrichomonas didelphidis (Trichomonadidae trichomonadinae) from the Didelphis marsupialis
TIANA TASCA,GERALDO A DE CARLI,MARIO STEINDEL,ROSSANA MATTIA
Parasitología al día , 1999,
Abstract: Tetratrichomonas didelphidis (Hegner and Ratcliffe, 1927) Andersen and Reilly, 1965 is a flagellate protozoan from the intestine, cecum, and colon of Didelphis marsupialis Linnaeus, 1758. The parasite was found and isolated in the rectal glands in Pavlova starch-containing media in Florianópolis, SC, Brasil from D.
Didelphis marsupialis como un reservorio potencial u hospedero amplificador del virus de la estomatitis vesicular, serotipo new jersey en Antioquia
Carlos Trujillo,John Arboleda
Iatreia , 2004,
Abstract: La Estomatitis Vesicular (EV) es una enfermedad viral, aguda y autolimitante que afecta principalmente bovinos, equinos y porcinos. Es producida por el virus de estomatitis vesicular (VEV), serotipos New Jersey (VEV-NJ) e Indiana (VEV-IN), que son los as importantes epidemiológicamente (1). Los estudios serológicos demuestran que VEV-NJ y VEV-IN infectan en forma natural una gran variedad de animales silvestres, que están posiblemente implicados en la coepizootiología de la EV, como hospederos portadores, mplificadores o reservorios (2). La zarigüeya (Didelphis marsupialis) es un buen candidato para cumplir esta función, debido a que es la especie silvestre mayormente capturada en zonas enzoóticas; presenta altos porcentajes de infección natural (3), resiste la antropización y además, su comportamiento le permite interactuar con diferentes poblaciones de vectores u otros reservorios en los bosques y servir como fuente de infección para las especies domésticas susceptibles.
Humoral Immune Response Kinetics in Philander opossum and Didelphis marsupialis Infected and Immunized by Trypanosoma cruzi Employing an Immunofluorescence Antibody Test
Legey Ana Paula,Pinho Ana Paula S,Xavier Samanta C Chagas,Leon Leonor L
Memórias do Instituto Oswaldo Cruz , 1999,
Abstract: Philander opossum and Didelphis marsupialis considered the most ancient mammals and an evolutionary success, maintain parasitism by Trypanosoma cruzi without developing any apparent disease or important tissue lesion. In order to elucidate this well-balanced interaction, we decided to compare the humoral immune response kinetics of the two didelphids naturally and experimentally infected with T. cruzi and immunized by different schedules of parasite antigens, employing an indirect fluorescence antibody test (IFAT). Both didelphids responded with high serological titers to different immunization routes, while the earliest response occurred with the intradermic route. Serological titers of naturally infected P. opossum showed a significant individual variation, while those of D. marsupialis remained stable during the entire follow-up period. The serological titers of the experimentally infected animals varied according to the inoculated strain. Our data suggest that (1) IFAT was sensitive for follow-up of P. opossum in natural and experimental T. cruzi infections; (2) both P. opossum and D. marsupialis are able to mount an efficient humoral immune response as compared to placental mammals; (3) experimentally infected P. opossum and D. marsupialis present distinct patterns of infection, depending on the subpopulation of T. cruzi, (4) the differences observed in the humoral immune responses between P. opossum and D. marsupialis, probably, reflect distinct strategies selected by these animals during their coevolution with T. cruzi.
Paragonimus mexicanus en hospederos naturales en el Estado Sucre, Venezuela.
Marcos Tulio Díaz y,Maira Marin,Erika Gómez,Antulio Prieto
Salus Online , 2011,
Abstract: Paragonimus mexicanus in natural hosts from two municipalities of the Sucre State, Venezuela.The Paragonimus mexicanus infection was studied in the opposum Didelphis marsupialis and crab Eudaniela garmani from the Sucre and Montes municipalities of Sucre State, Venezuela. In Sucre,prevalence of adult parasites in opposums was 13. 33% with an intensity of 1.50 parasites per individual; in Montes, prevalence was 31.88% with a parasite load of 6.82 parasites per host. Prevalence of metacercariae in crabs was 17. 98% in Montes and 4.07% in Sucre. No significant differences between prevalence, intensity and gender of the crabs was found in any of the two areas, but there was a positive and significant correlation with size. In Sucre, the crab parasite burden was higher, 8.20%, than in Montes,5.31%. It was concluded that the intensity of infection was higher in female crabs and in those of a larger body size. These results indicate the existence of zoonotic contamination in both areas, and the need tocontinue investigating in order to determine relevant elements of this parasitic interrelation.
Inmunodiagnóstico en Didelphis marsupialis usando un antígeno de Paragonimus de Venezuela Immunodiagnostic in Didelphis marsupialis using an antigen of Venezuelan Paragonimus
Erika Gómez Martínez,Zoraida Díaz-Bello,Reinaldo Zavala-Jaspe,Marcos Tulio Díaz
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: Los Paragonimus son trematodos que habitualmente viven en los pulmones de mamíferos carnívoros y omnívoros, entre ellos el hombre. En el oriente venezolano se encuentra el único foco de Paragonimus sp. donde Didelphis marsupialis es el único reservorio demostrado hasta ahora. Con el fin de tener herramientas de inmunodiagnóstico que detecten la presencia de Paragonimus sp. en esta especie, se elaboraron varios reactivos para realizar un ensayo inmunoenzimático ELISA. Entre ellos se obtuvo un antígeno crudo soluble de vermes adultos de Paragonimus y una inmunoglobulina de gallina anti-IgG de Didelphis marsupialis. Los mismos se capturaron en la localidad de Aguas Blancas, municipio Montes, estado Sucre, Venezuela, y se obtuvieron muestras sanguíneas; en el caso de estar infectados, los vermes adultos se extrajeron del pulmón. Los parásitos se homogenizaron y ultracentrifugaron para obtener la fracción soluble del parásito (FSPA) como antígeno para el ELISA y Western blot y detectar los anticuerpos en los Didelphis marsupialis. El análisis electroforético mostró 22 moléculas entre 6 y 82 kDa; por Western blot se presentó un reconocimiento antigénico de 8 moléculas siendo las de 112 kDa y 268 kDa las más reconocidas por los sueros positivos. Los sueros negativos no reconocieron ninguna proteína. La producción de IgY en gallinas permitió desarrollar las técnicas de inmunodiagnóstico para la búsqueda de anticuerpos específicos anti-Paragonimus sp. en Didelphis, cuya aplicación permitirá establecer la vigilancia epidemiológica de estos reservorios en áreas endémicas sin sacrificio de los mismos. Paragonimus are trematodes that normally live in the lungs of carnivorous and omnivorous mammals such as humans. An outbreak of Paragonimus sp. in which Didelphis marsupialis was the only wild reservoir incriminated was described in eastern Venezuela. In order to have immunological tools to detect the presence of Paragonimus sp. in this reservoir, a whole antigen of the adult worm of this parasite was elaborated. Didelphis marsupialis were captured in the locality of Aguas Blancas, Montes municipality, Sucre state, Venezuela, from which blood samples were obtained and a search for worms was performed in lungs. Worms were homogenized and ultracentrifugated to obtain FSPA to perform immunoassay (ELISA) to detect antibodies in opossums. The electrophoresis analysis showed a pattern of 22 molecules between 6 and 82 kDa; by western blot, the antigenic recognition of 8 antigenic molecules appeared,112 kDa and 268 kDa molecules being the most strongly recognized by positive
Morphologic aspects of Tetratrichomonas didelphidis isolated from opossums Didelphis marsupialis and Lutreolina crassicaudata
Tasca, Tiana;De Carli, Geraldo Attilio;Glock, Luiz;Jeckel-Neto, Emílio Ant?nio;
Memórias do Instituto Oswaldo Cruz , 2001, DOI: 10.1590/S0074-02762001000200022
Abstract: tetratrichomonas didelphidis (hegner & ratcliffe, 1927) andersen & reilly, 1965 is a flagellate protozoan found in the intestine, cecum, and colon of didelphis marsupialis. the parasitic protozoa used in this study was found and isolated in the intestine of opossums in pavlova starch-containing medium in florianópolis, state of santa catarina, brazil, from d. marsupialis and lutreolina crassicaudata. the strains were cultivated in diamond medium without maltose and with starch solution, ph 7.5 at 28°c. the specimens were stained by the giemsa method and heidenhain's iron hematoxylin. the light microscopy study of the trophozoites revealed the same morphologic characteristics as specimens previously described.
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