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Genetic Linkage Map and Comparative Genome Analysis for the Atlantic Killifish (Fundulus heteroclitus)  [PDF]
Eric R. Waits, John Martinson, Brian Rinner, Stephen Morris, Dina Proestou, Denise Champlin, Diane Nacci
Open Journal of Genetics (OJGen) , 2016, DOI: 10.4236/ojgen.2016.61004
Abstract: Genetic linkage maps are valuable tools in evolutionary biology; however, their availability for wild populations is extremely limited. Fundulus heteroclitus (Atlantic killifish) is a non-migratory estuarine fish that exhibits high allelic and phenotypic diversity partitioned among subpopulations that reside in disparate environmental conditions. An ideal candidate model organism for studying gene-environment interactions, the molecular toolbox for F. heteroclitus is limited. We identified hundreds of novel microsatellites which, when combined with existing microsatellites and single nucleotide polymorphisms (SNPs), were used to construct the first genetic linkage map for this species. By integrating independent linkage maps from three genetic crosses, we developed a consensus map containing 24 linkage groups, consistent with the number of chromosomes reported for this species. These linkage groups span 2300 centimorgans (cM) of recombinant genomic space, intermediate in size relative to the current linkage maps for the teleosts, medaka and zebrafish. Comparisons between fish genomes support a high degree of synteny between the consensus F. heteroclitus linkage map and the medaka and (to a lesser extent) zebrafish physical genome assemblies.
Il significato delle rovine italiane. Viaggiatori tra Europa e America da Tocqueville a James
Michela Nacci
Scienza & Politica : per una Storia delle Dottrine , 2002, DOI: 10.6092/issn.1825-9618/2889
Abstract: Il significato delle rovine italiane. Viaggiatori tra Europa e America da Tocqueville a James
Population genetic diversity and fitness in multiple environments
Jeffrey A Markert, Denise M Champlin, Ruth Gutjahr-Gobell, Jason S Grear, Anne Kuhn, Thomas J McGreevy, Annette Roth, Mark J Bagley, Diane E Nacci
BMC Evolutionary Biology , 2010, DOI: 10.1186/1471-2148-10-205
Abstract: Populations with very low genetic diversity demonstrated reduced fitness relative to high diversity populations even under permissive conditions. Population performance decreased in the stressful environment for all levels of genetic diversity relative to performance in the permissive environment. Twenty percent of the lowest diversity populations went extinct before the end of the study in permissive conditions, whereas 73% of the low diversity lines went extinct in the stressful environment. All high genetic diversity populations persisted for the duration of the study, although population sizes and reproduction were reduced under stressful environmental conditions. Levels of fitness varied more among replicate low diversity populations than among replicate populations with high genetic diversity. There was a significant correlation between AFLP diversity and population fitness overall; however, AFLP markers performed poorly at detecting modest but consequential losses of genetic diversity. High diversity lines in the stressful environment showed some evidence of relative improvement as the experiment progressed while the low diversity lines did not.The combined effects of reduced average fitness and increased variability contributed to increased extinction rates for very low diversity populations. More modest losses of genetic diversity resulted in measurable decreases in population fitness; AFLP markers did not always detect these losses. However when AFLP markers indicated lost genetic diversity, these losses were associated with reduced population fitness.Decreased population genetic diversity can be associated with declines in population fitness (e.g., [1,2]). These declines are thought to involve components of the so called genetic 'extinction vortex', which directly ties losses in population genetic diversity to increased extinction risk [3]. These losses cause a decrease in individual fitness through the expression of inbreeding depression-like effects, fu
Special Issue: Nano-Catalysts and Nano-Technologies for Green Organic Synthesis
Angelo Nacci,Nicola Cioffi
Molecules , 2011, DOI: 10.3390/molecules16021452
Abstract: Catalysis by transition-metal nanoparticles has undergone an explosive growth during the past decade. This special issue presents the general trends in the current research in this field, the present situation concerning scope and limitations, as well as the future perspectives. Original contributions are also presented on the applications of nano-catalysts to the green synthesis.
Introduction to Forensic Dentistry Continuing Education Course  [PDF]
Diane Osborne
Forensic Medicine and Anatomy Research (FMAR) , 2013,
Abstract: This course is an introduction to the basics of forensic dentistry beginning with its historical origins to modern advancements. After an introduction to basic principles, application of this information will be demonstrated in current cases, training in mass fatalities and participation in a mass fatality workshop scenario using dry skull remains. Time permitting, a tour of the Las Vegas Coroner’s Office will be available.
Changing Hostile Beliefs towards Women through Partner Abuse Intervention Programs  [PDF]
Diane Zosky
Open Journal of Social Sciences (JSS) , 2016, DOI: 10.4236/jss.2016.42014
Abstract: This research project examined hostile beliefs towards women held by men who completed a Duluth Model partner violence intervention program. Changes in beliefs are foundational to change in the cessation of violence within the Duluth model. Hostile beliefs towards women establish a context for objectification and suspicion of women, and justification for violence. Although there was not a significant reduction in hostility scores, there was a significant reduction in aggression scores. Hostility scores were significantly correlated with the aggression scores indicating encouraging results that partner abuse intervention programs may influence the reduction of hostile beliefs towards women and thus may impact perpetration of violence.
Key Vulnerabilities and Limitations in the Management of Hazardous Waste and Its Disposal: A Checklist Assessment Tool  [PDF]
Rob White, Diane Heckenberg
Journal of Environmental Protection (JEP) , 2011, DOI: 10.4236/jep.2011.29145
Abstract: From an environmental protection perspective, the crucial issues pertaining to the policing of hazardous waste relate to both the vulnerabilities and limitations of current practices, and the potential issues that demand attention in the here and now, to alleviate future calamity. This paper describes the process involved in developing a vulnerabilities and limitations checklist that provides a relatively simple yet multi-pronged approach to assessing present and future environmental harms and crimes within the hazardous waste sector. Although it was not the intention of the authors to develop a generic checklist, this tool may prove useful to other industry sectors.
The VL2-Spoken Language Phonological Awareness (VL2-SLPA) Measure  [PDF]
M. Diane Clark
Psychology (PSYCH) , 2012, DOI: 10.4236/psych.2012.310137
Abstract: Tests of phonological awareness have been developed for spoken languages that require spoken responses. For many deaf individuals, spoken measures of phonological awareness (PA) are not appropriate, as these deaf individuals do not use any spoken language or their oral language is rated as low on levels of aural comprehension. Given the need to have accessible measures of spoken language PA for deaf children, the VL2 Spoken Language Phonological Awareness Measure (VL2-SLPA) was developed. The VL2-SLPA can also determine if participants use a phonological code or an orthographic code to identify the two pictures that have the same first or last “sound”. The VL2-SLPA showed strong convergent validity to the Phoneme Detection Test, another measure developed for deaf individuals, which does not require a verbal response.
How many cases of laryngopharyngeal reflux suspected by laryngoscopy are gastroesophageal reflux disease-related?
Nicola de Bortoli,Andrea Nacci,Edoardo Savarino,Irene Martinucci
World Journal of Gastroenterology , 2012, DOI: 10.3748/wjg.v18.i32.4363
Abstract: AIM: To investigate the prevalence of gastroesophageal reflux disease (GERD) in patients with a laryngoscopic diagnosis of laryngopharyngeal reflux (LPR). METHODS: Between May 2011 and October 2011, 41 consecutive patients with laryngopharyngeal symptoms (LPS) and laryngoscopic diagnosis of LPR were empirically treated with proton pump inhibitors (PPIs) for at least 8 wk, and the therapeutic outcome was assessed through validated questionnaires (GERD impact scale, GIS; visual analogue scale, VAS). LPR diagnosis was performed by ear, nose and throat specialists using the reflux finding score (RFS) and reflux symptom index (RSI). After a 16-d wash-out from PPIs, all patients underwent an upper endoscopy, stationary esophageal manometry, 24-h multichannel intraluminal impedance and pH (MII-pH) esophageal monitoring. A positive correlation between LPR diagnosis and GERD was supposed based on the presence of esophagitis (ERD), pathological acid exposure time (AET) in the absence of esophageal erosions (NERD), and a positive correlation between symptoms and refluxes (hypersensitive esophagus, HE). RESULTS: The male/female ratio was 0.52 (14/27), the mean age ± SD was 51.5 ± 12.7 years, and the mean body mass index was 25.7 ± 3.4 kg/m2. All subjects reported one or more LPS. Twenty-five out of 41 patients also had typical GERD symptoms (heartburn and/or regurgitation). The most frequent laryngoscopic findings were posterior laryngeal hyperemia (38/41), linear indentation in the medial edge of the vocal fold (31/41), vocal fold nodules (6/41) and diffuse infraglottic oedema (25/41). The GIS analysis showed that 10/41 patients reported symptom relief with PPI therapy (P < 0.05); conversely, 23/41 did not report any clinical improvement. At the same time, the VAS analysis showed a significant reduction in typical GERD symptoms after PPI therapy (P < 0.001). A significant reduction in LPS symptoms. On the other hand, such result was not recorded for LPS. Esophagitis was detected in 2/41 patients, and ineffective esophageal motility was found in 3/41 patients. The MII-pH analysis showed an abnormal AET in 5/41 patients (2 ERD and 3 NERD); 11/41 patients had a normal AET and a positive association between symptoms and refluxes (HE), and 25/41 patients had a normal AET and a negative association between symptoms and refluxes (no GERD patients). It is noteworthy that HE patients had a positive association with typical GERD-related symptoms. Gas refluxes were found more frequently in patients with globus (29.7 ± 3.6) and hoarseness (21.5 ± 7.4) than in patients with he
Perceptions of cultural competency of undergraduate nursing students  [PDF]
Diane Von Ah, Natasha Cassara
Open Journal of Nursing (OJN) , 2013, DOI: 10.4236/ojn.2013.32024

Purpose: The purpose of this study was to examine the level of cultural competence of undergraduate nursing students at a large Midwestern University. Background: Developing cultural competence of nurses is critical to meet the needs of our growing diverse society. Methods: A convenience sample of 150 undergraduate nursing students completed a one-time questionnaire assessing students’ cultural competence. Results: Nursing students’ rated their overall knowledge and comfort moderate to poor with only 28% rated themselves as very comfortable and 15% as very skillful in providing culturally competent care. Students rated their knowledge and comfort highest in taking a family history, whereas, the lowest scores were related to pregnancy and childbirth, death and dying, and organ donation in other cultures. Conclusions: Findings suggest that nursing students feel inadequately prepared to provide culturally competent care.

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