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Search Results: 1 - 10 of 16586 matches for " Der-Chih Liao "
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Twist Controls Skeletal Development and Dorsoventral Patterning by Regulating Runx2 in Zebrafish
Der-Chih Yang, Chih-Chien Tsai, Yun-Feng Liao, Hui-Chuan Fu, Huey-Jen Tsay, Tung-Fu Huang, Yau-Hung Chen, Shih-Chieh Hung
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0027324
Abstract: Background Twist1a and twist1b are the principal components of twists that negatively regulate a number of cellular signaling events. Expression of runx2 and downstream targets is essential for skeletal development and ventral organizer formation and specification in early vertebrate embryos, but what controls ventral activity of maternal runx2 and how twists function in zebrafish embryogenesis still remain unclear. Methodology/Principal Findings By studying the loss of twist induced by injection of morpholino-oligonucleotide in zebrafish, we found that twist1a and twist1b, but not twist2 or twist3, were required for proper skeletal development and dorsoventral patterning in early embryos. Overexpression of twist1a or twist1b following mRNA injection resulted in deteriorated skeletal development and formation of typical dorsalized embryos, whereas knockdown of twist1a and twist1b led to the formation of abnormal embryos with enhanced skeletal formation and typical ventralized patterning. Overexpression of twist1a or twist1b decreased the expression of runx2b, whereas twist1a and twist1b knockdown increased runx2b expression. We have further demonstrated that phenotypes induced by twist1a and twist1b knockdown were rescued by runx2b knockdown. Conclusions/Significance Together, these results suggest that twist1a and twist1b control skeletal development and dorsoventral patterning by regulating runx2b in zebrafish and provide potential targets for the treatment of diseases or syndromes associated with decreased skeletal development.
Complete Chloroplast Genome Sequence of an Orchid Model Plant Candidate: Erycina pusilla Apply in Tropical Oncidium Breeding
I-Chun Pan, Der-Chih Liao, Fu-Huei Wu, Henry Daniell, Nameirakpam Dolendro Singh, Chen Chang, Ming-Che Shih, Ming-Tsair Chan, Choun-Sea Lin
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0034738
Abstract: Oncidium is an important ornamental plant but the study of its functional genomics is difficult. Erycina pusilla is a fast-growing Oncidiinae species. Several characteristics including low chromosome number, small genome size, short growth period, and its ability to complete its life cycle in vitro make E. pusilla a good model candidate and parent for hybridization for orchids. Although genetic information remains limited, systematic molecular analysis of its chloroplast genome might provide useful genetic information. By combining bacterial artificial chromosome (BAC) clones and next-generation sequencing (NGS), the chloroplast (cp) genome of E. pusilla was sequenced accurately, efficiently and economically. The cp genome of E. pusilla shares 89 and 84% similarity with Oncidium Gower Ramsey and Phalanopsis aphrodite, respectively. Comparing these 3 cp genomes, 5 regions have been identified as showing diversity. Using PCR analysis of 19 species belonging to the Epidendroideae subfamily, a conserved deletion was found in the rps15-trnN region of the Cymbidieae tribe. Because commercial Oncidium varieties in Taiwan are limited, identification of potential parents using molecular breeding method has become very important. To demonstrate the relationship between taxonomic position and hybrid compatibility of E. pusilla, 4 DNA regions of 36 tropically adapted Oncidiinae varieties have been analyzed. The results indicated that trnF-ndhJ and trnH-psbA were suitable for phylogenetic analysis. E. pusilla proved to be phylogenetically closer to Rodriguezia and Tolumnia than Oncidium, despite its similar floral appearance to Oncidium. These results indicate the hybrid compatibility of E. pusilla, its cp genome providing important information for Oncidium breeding.
Hypoxia Inhibits Osteogenesis in Human Mesenchymal Stem Cells through Direct Regulation of RUNX2 by TWIST
Der-Chih Yang, Muh-Hwa Yang, Chih-Chien Tsai, Tung-Fu Huang, Yau-Hung Chen, Shih-Chieh Hung
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0023965
Abstract: Background Bone loss induced by hypoxia is associated with various pathophysiological conditions, however, little is known about the effects of hypoxia and related signaling pathways on osteoblast differentiation and bone formation. Because bone marrow-derived mesenchymal stem cells (MSCs) survive under hypoxic conditions and readily differentiate into osteoblasts by standard induction protocols, they are a good in vitro model to study the effects of hypoxia on osteoblast differentiation. Methodology/Principle Findings Using human MSCs, we discovered TWIST, a downstream target of HIF-1α, was induced under hypoxia and acted as a transcription repressor of RUNX2 through binding to the E-box located on the promoter of type 1 RUNX2. Suppression of type 1 RUNX2 by TWIST under hypoxia further inhibited the expression of BMP2, type 2 RUNX2 and downstream targets of RUNX2 in MSCs. Conclusions/Significance Our findings point to the important role of hypoxia-mediated signalling in osteogenic differentiation in MSCs through direct regulation of RUNX2 by TWIST, and provide a method for modifying MSC osteogenesis upon application of these cells in fracture healing and bone reconstruction.
cAMP/PKA Regulates Osteogenesis, Adipogenesis and Ratio of RANKL/OPG mRNA Expression in Mesenchymal Stem Cells by Suppressing Leptin
Der-Chih Yang, Huey-Jen Tsay, Shan-Yang Lin, Shih-Hwa Chiou, Mei-Jane Li, Tai-Jay Chang, Shih-Chieh Hung
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0001540
Abstract: Background Mesenchymal stem cells (MSCs) are a pluripotent cell type that can differentiate into adipocytes, osteoblasts and other cells. The reciprocal relationship between adipogenesis and osteogenesis was previously demonstrated; however, the mechanisms remain largely unknown. Methods and Findings We report that activation of PKA by 3-isobutyl-1 methyl xanthine (IBMX) and forskolin enhances adipogenesis, the gene expression of PPARγ2 and LPL, and downregulates the gene expression of Runx2 and osteopontin, markers of osteogenesis. PKA activation also decreases the ratio of Receptor Activator of the NF-κB Ligand to Osteoprotegerin (RANKL/OPG) gene expression – the key factors of osteoclastogenesis. All these effects are mediated by the cAMP/PKA/CREB pathway by suppressing leptin, and may contribute to PKA stimulators-induced in vivo bone loss in developing zebrafish. Conclusions Using MSCs, the center of a newly proposed bone metabolic unit, we identified cAMP/PKA signaling, one of the many signaling pathways that regulate bone homeostasis via controlling cyto-differentiation of MSCs and altering RANKL/OPG gene expression.
A process simplification scheme for fabricating CMOS polycrystalline-Si thin film transistors

Juang Miin-Horng,Chang Chia-Wei,Shye Der-Chih,Hwang Chuan-Chou,Wang Jih-Liang,Jang Sheng-Liang,

半导体学报 , 2010,
Abstract: A process simplification scheme for fabricating CMOS poly-Si thin-film transistors (TFTs) has been proposed, which employs large-angle-tilt-implantation of dopant through a gate sidewall spacer (LATITS). By this LATITS scheme, a lightly doped drain region under the oxide spacer is formed by low-dose tilt implantation of phosphorus (or boron) dopant through the spacer, and then the nC-source/drain (nC-S/D) (or pC-S/D) region is formed via using the same photo-mask layer during CMOS integration. For both n-TFT and p-TFT devices, as compared to the sample with conventional single nC-S/D (or pC-S/D) structure, the LATITS scheme can cause an obviously smaller leakage current, due to more gradual dopant distribution and thus smaller electric field. In addition, the resultant on-state currents only show slight degradation for the LATITS scheme. As a result, by the LATITS scheme, CMOS poly-Si TFT devices with an on/off current ratio well above 8 orders may be achieved without needing extra photo-mask layers during CMOS integration.
Electrolysis of nano-Silver Suspension into Ionic Form via Membrane Electro-Osmosis Process
Kuo Hsiung Tseng,Chih Yu Liao,Der Chi Tien,Tsing Tshih Tsung
Lecture Notes in Engineering and Computer Science , 2008,
A Study to Compare the Properties of Nano-Gold Suspension Fabricated by Spark Discharge System in Different Dielectric Media
Der Chi Tien,Kuo Hsiung Tseng,Chih Yu Liao,Jen-Chuen Huang
Lecture Notes in Engineering and Computer Science , 2008,
Erythropoiesis Suppression Is Associated with Anthrax Lethal Toxin-Mediated Pathogenic Progression
Hsin-Hou Chang, Tsung-Pao Wang, Po-Kong Chen, Yo-Yin Lin, Chih-Hsien Liao, Ting-Kai Lin, Ya-Wen Chiang, Wen-Bin Lin, Chih-Yu Chiang, Jyh-Hwa Kau, Hsin-Hsien Huang, Hui-Ling Hsu, Chi-Yuan Liao, Der-Shan Sun
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0071718
Abstract: Anthrax is a disease caused by the bacterium Bacillus anthracis, which results in high mortality in animals and humans. Although some of the mechanisms are already known such as asphyxia, extensive knowledge of molecular pathogenesis of this disease is deficient and remains to be further investigated. Lethal toxin (LT) is a major virulence factor of B. anthracis and a specific inhibitor/protease of mitogen-activated protein kinase kinases (MAPKKs). Anthrax LT causes lethality and induces certain anthrax-like symptoms, such as anemia and hypoxia, in experimental mice. Mitogen-activated protein kinases (MAPKs) are the downstream pathways of MAPKKs, and are important for erythropoiesis. This prompted us to hypothesize that anemia and hypoxia may in part be exacerbated by erythropoietic dysfunction. As revealed by colony-forming cell assays in this study, LT challenges significantly reduced mouse erythroid progenitor cells. In addition, in a proteolytic activity-dependent manner, LT suppressed cell survival and differentiation of cord blood CD34+-derived erythroblasts in vitro. Suppression of cell numbers and the percentage of erythroblasts in the bone marrow were detected in LT-challenged C57BL/6J mice. In contrast, erythropoiesis was provoked through treatments of erythropoietin, significantly ameliorating the anemia and reducing the mortality of LT-treated mice. These data suggested that suppressed erythropoiesis is part of the pathophysiology of LT-mediated intoxication. Because specific treatments to overcome LT-mediated pathogenesis are still lacking, these efforts may help the development of effective treatments against anthrax.
Gender Difference of Alanine Aminotransferase Elevation May Be Associated with Higher Hemoglobin Levels among Male Adolescents
Solomon Chih-Cheng Chen,Jun-Jun Yeh,Mei-Hwei Chang,Yu-Kuei Liao,Li-Chen Hsiao,Choo-Aun Neoh,Teck-Siang Tok,Jung-Der Wang
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0013269
Abstract: To explore the gender difference of ALT elevation and its association with high hemoglobin levels.
Mechanisms and Immune Dysregulation in Arsenic Skin Carcinogenesis  [PDF]
Chih-Hung Lee, Wei-Ting Liao, Hsin-Su Yu
Journal of Cancer Therapy (JCT) , 2010, DOI: 10.4236/jct.2010.12013
Abstract: Long-term exposure to arsenic is associated with cancers of lung, urinary bladder, kidney, liver and skin. Arsenic car-cinogenesis might result from oxidative stress, altered growth factors, chromosomal abnormality, immune dysregula-tion, and aberrant epigenetic regulations. Bowen’s disease (As-BD) is the most common form of arsenic-induces skin cancers and is characterized by chronicity, multiplicity, and predisposition in sun-spare skin. However, only about 1% of the population exposed to arsenic developped skin cancers, indicating the host immune response plays an important modulatory role in skin carcinogenesis. In this review, we review the pathomechanisms of arsenic skin carcinogenesis and the immune interactions. Arsenic affects innate and adaptive immune responses through CD4+ T cells, monocytes, macrophages, and Langerhans cells. In skin of As-BD, CD4+ T cells undergo selective and differential apoptosis via Fas-FasL interaction. Numbers and dendrites of Langerhans cells are reduced in As-BD lesions. There is a defective homeostasis and aberrant trafficking of Langerhans cells. Such information is essential to understand the molecular mechanism for arsenic carcinogenesis in both skin and in internal organs.
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