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Search Results: 1 - 10 of 199152 matches for " Demetrius Ant?nio Machado de Araújo "
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Influence of Sub-Lethal and Lethal Concentrations of Chlorhexidine on Morphology and Glucosyltransferase Genes Expression in Streptococcus mutans UA159  [PDF]
Andréa Cristina Barbosa da Silva, Rafael Nóbrega Stipp, Renata de Oliveira Mattos-Graner, Fábio Correia Sampaio, Demetrius Antnio Machado de Araújo
Advances in Microbiology (AiM) , 2014, DOI: 10.4236/aim.2014.413105
Abstract: Chlorhexidine (CHX) is regarded as one of the most successful antiplaque agents in controlling the formation of dental biofilm. Nevertheless, molecular mechanisms of their effects in Streptococcus mutans are largely unknown. In this work, the effects of sub-lethal and lethal concentrations of chlorhexidine (CHX) on planktonic or biofilm-organized Streptococcus mutans cells were investigated in dose- and time-dependent manner. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) for planktonic cells and biofilm conditions were determined by standard methods. Quantitative PCR (qPCR) was used to quantify the relative levels of glucosyltransferase B (gtfB), gtfC and gtfD transcription of S. mutans in the presence of CHX. The CHX activity in the initial biofilm structure and morphological alterations in planktonic cells were examined by Scanning Electron Microscopy (SEM). The results indicate that CHX increased expression of gtfC and gtfD in planktonic S. mutans cells and CHX reduced the expression of gtfB, gtfC, and gtfD in biofilms. High concentrations of CHX resulted in several wilted S. mutans planktonic cells with spilled intracellular material, while decreased cells’ chain length and matrix was found when the initial biofilm was exposed to increasing concentrations of CHX. CHX’s effects against bacteria depend on the type of growth organization and the concentration and time of exposure to the drug. At sub-lethal concentrations, CHX affects the expression of glucosyltransferases, which may have anticariogenic effect.
Detection of oral streptococci in dental biofilm from caries-active and caries-free children
Silva, Andréa Cristina Barbosa da;Cruz, Jader dos Santos;Sampaio, Fábio Correia;Araújo, Demetrius Antnio Machado de;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000400009
Abstract: this work correlated the presence of oral streptococci in dental biofilm with clinical indexes of caries and oral hygiene in caries-active and caries-free children. s. mutans and/or s. sobrinus in the dental biofilm does not indicate a direct risk for developing dental caries.
Efeito da atmosfera modificada ativa na qualidade do mel?o 'orange flesh' minimamente processado
Araújo, Francisca Marta Machado Casado de;Machado, Antnio Vitor;Chitarra, Adimilson Bosco;
Ciência e Agrotecnologia , 2005, DOI: 10.1590/S1413-70542005000400014
Abstract: the increase of the demand for products processed minimally brings a great challenge for the food science and technology, being considered the shortage of information about the maintenance of the quality of those products. the storage of these products in appropriate conditions is a fundamental point for the success of this technology. the objective of this work was to evaluate the effect of the active modified atmosphere in the quality and conservation of the melon 'orange flesh' processed minimally. the fruits, after the processing were wrapped under modified atmosphere (passive am control; active am with 5% of co2 and 5% of o2 and activate am with 10% of co2 and 2% of o2), stored at (6oc ± 1oc and r.h. 85% ± 5%) during 8 days and the retired samples for analyses physics, physical-chemistries, chemistries and sensorial every 2 days of storage. the variables texture, flavor, acidity titratable and total pectin, they were not affected for the active modified atmosphere. smaller solubilization of pectins and larger retention of neutral sugars, they were detected in melons under atmosphere with 10% of co2 and 2% of o2. the quality attributes, flavor and texture did not suffer deteriorations during the 8 days of storage, in all the treatments, in agreement with the sensorial analysis.
A influência de duas fra??es inspiradas de oxigênio no padr?o respiratório de pacientes sob desmame ventilatório
Diniz, Gisele do Carmo Leite Machado;Zin, Walter Araújo;Botoni, Fernando Antnio;Castro, Aldemar Vilela de;Rodrigues-Machado, Maria da Glória;
Revista Brasileira de Terapia Intensiva , 2009, DOI: 10.1590/S0103-507X2009000300009
Abstract: background and objectives: an inspired oxygen fraction (fio2) of 40% is often used for weaning patients, but lower fio2 values are also recommended, if arterial oxygen pressure (pao2)/ fio2 >150-200 mmhg. this study aimed to compare respiratory variables and vital data values recorded during use of sufficient fio2 (ideal) to maintain peripheral oxygen saturation at 92% with values recorded during use of fio2 established at 40% (baseline) in weaning patients. methods: prospective cross-over study. respiratory variables (respiratory frequency, tidal volume, occlusion pressure, inspiratory time/total time ratio) and vital data (blood pressure and heart rate) were collected sequentially at 30 and 60 minutes with baseline fio2, followed by ideal fio2. these were compared to a generalized linear model for repeated measurements. comparisons between baseline and ideal fio2 values, and arterial blood gases were evaluated by the student's t or wilcoxon tests. results: in 30 adult patients the median of ideal fio2 was 25% (iq25%-75% 23-28). this was significantly lower than baseline fio2 (40%) (p< 0.001). no significant difference was found in the pao2/ fio2 ratio between baseline fio2 (269±53) and ideal fio2 (268±47). tidal volume was significantly lower during use of ideal fio2 (p=0.003) and blood pressure was significantly higher during use of baseline fio2 (p=0.041), but there was no clinical significance. the remaining variables were not affected by reduction in fio2. the ideal fio2 did not influence remaining variables. conclusions: these results suggest that fio2 levels sufficient to ensure a spo2>92% did not alter breathing patterns or trigger clinical changes in weaning patients.
Peso do ba?o como fator prognóstico do tratamento cirúrgico de pacientes portadores de esquistossomose mans?nica
Ferraz, álvaro Antnio Bandeira;Lopes, Edmundo Pessoa de Almeida;Araújo Júnior, José Guido Corrêa de;Lima, Bruno de Andrade;Cantarelli, Fabiano;Ferraz, Edmundo Machado;
Revista do Colégio Brasileiro de Cirurgi?es , 2002, DOI: 10.1590/S0100-69912002000100007
Abstract: background: searching for that risk factors that could commit surgical treatment of patient with schistosomiasis, the authors analyzed the spleen as a prognostic factor. methods: between 1992 and 1998, 114 patients underwent splenectomy with ligation of the left gastric vein (llgv) and devascularisation of the great stomach curvature, followed by post-operative endoscopic sclerotherapy for the treatment of hepatic-splenic schistosomiasis with previous gastrointestinal haemorrhages. a clinical/laboratorial analysis was performed. patients were divided into 3 groups according to the weight of the spleen, in the moment of the accomplishment of the histologycal cuts: group i - spleen smaller than 500 grams (17); group ii - spleen between 500 and 1000 grams (58) and; group iii - spleen greater than 1000 grams (39). results: patients of group i presented rebleeding rate of 17,6%, thrombosis of the portal vein of 5,9% and there was not mortality. the incidence of preoperative hipersplenism was 29,4% and the caliber of the portal vein was 1,1 cm. in group ii the rebleeding rate was of 15,5%, thrombosis of the portal vein of 10,3% and the mortality incidence of hiperplenism of 4 patient (6,9%), 2 patients in the immediate postoperative period and 2 in the late followup (hepatocarcinoma and digestive hemorrhage). the hipersplenism incidence was of 53,4% and preoperative mean caliber of the portal vein was 1,4cm. in group iii the rebleeding incidence was 12,8%, thrombosis of the portal vein 5,1% and a late mortality of 2 patient (linfoma and miocardium infarct). the hipersplenism incidence was of 76,9% and the caliber of the portal vein was of 1,5 cm. conclusions: weight of the spleen presents relationship with preoperative hipersplenism, with portal vein caliber and postoperative hospitalar stay. there was no relationship with fundus gastric varices, rebleeding rate, portal vein thrombosis, degree of periportal fibrosis and biochemical data.
Varizes de fundo gástrico na hipertens?o portal esquistossomótica: resultados cirúrgicos
Ferraz, álvaro Antnio Bandeira;Lopes, Edmundo Pessoa de Almeida;Araújo Júnior, José Guido Corrêa de;Lima, Bruno de Andrade;Cantarelli, Fabiano;Ferraz, Edmundo Machado;
Revista do Colégio Brasileiro de Cirurgi?es , 2003, DOI: 10.1590/S0100-69912003000100005
Abstract: background: the aim of this study is to present epidemiological data and evaluate a surgical approach in the treatment of gastric fundus varices in patients with hepatosplenic shistosomiasis. methods: during the period of january 1992 and july 2001, 125 patients underwent splenectomy, ligation of the left gastric vein (llgv), devascularization of the great stomach curvature and post-operative endoscopic sclerotherapy for the treatment of hepatic-splenic schistosomiasis with previous gastrointestinal haemorrhages. in the patients who presented gastric varices in the pre-operative endoscopy (44/125), a gastrotomy and an obliterating running suture were also performed intraoperatively. results: gastric fundus varices were observed in 35,2% of all patients with hepatic-splenic schistosomiasis with previous gastrointestinal haemorrhages (44/125). the surgical treatment proposed eradicated 76,5% of the gastric fundus varices in a mean follow-up period of 26 months. portal vein thrombosis was higher in the group of patients without fundus grastric varices (16,3%) when compared with fundus gastric varices patients (8,8%). this difference was not statistically significant (p=0,62). there was no correlation between the presence of fundus gastric varices and the degree of periportal fibrosis or the weight of the spleen. despite a statistically lower number of white blood cells in the gastric fundus varices, no other differences were identified in the preoperative haematological and biochemical data. conclusions: the authors concluded that patients underwent splenectomy, ligation of the left gastric vein, devascularisation of the great stomach curvature, post-operative endoscopic sclerotherapy, gastrotomy and an obliterating running suture of the fundus gastric varices, eradicated 76,5% of the fundus gastric varices, in a follow-up of 26 months.
Atividade mioelétrica do intestino delgado de c?es submetidos à oclus?o parcial da veia porta
Ferraz, álvaro Antnio Bandeira;Campos, Josemberg Marins;Evangelista, Luis Fernando;Coelho, Antonio Roberto de Barros;Araújo-Filho, José Guido de;Ferraz, Edmundo Machado;
ABCD. Arquivos Brasileiros de Cirurgia Digestiva (S?o Paulo) , 2011, DOI: 10.1590/S0102-67202011000100005
Abstract: background: temporary occlusion of the portal vein causes splancnic venous stasis and intestinal disfunction, that can produce alterations in the motility, and this fact is not vastly known. aim: to evaluate the small bowel myoelectric activity and histology in the six dogs, also covering mean arterial blood pressure (ap), pulse rate (pr), central venous pressure (cvp) e portal pressure (pp), in two phases: pre-occlusion and occlusion. methods: it was done general anesthesia, invasive monitorization of the ap, pr e cvp, laparotomy, measure of the pp, fixation of the three pairs of electrodes in the intestinal wall, jejunal biopsy and parcial occlusion of the portal vein, being programmed the increase of the pp between 2.5 and 3 times baseline. electrodes were connected to a computer system that captured electrical signals from the intestine. the computer has a software of acquisition to store and analyse the myoelectric activity after registering, what happened in 30 minutes of the pre-occlusion phase and in 60 minutes of occlusion. the variance and the mean rms(root mean square) of the myoeletric activity were determined. the statistical analysis was done with friedman, dunn, cochran and students t tests. results: mean rms of myoeletric activity showed significant decrease in the phase of portal occlusion, in comparison to preocclusion phase. the frequency of hemorrhage of the lamina propria was major during occlusion. it was proportional at stasis time, with significant difference between the preocclusion and the occlusion of 60 minutes. inflammatory infiltration, vascular dilation and epithelial detachment did not show any differences between two phases. during the stasis, ap, pr and cvp decreased (p=0,326; 0,375 e 0,008, respectively), and pp increased(p=0,015). conclusions: parcial occlusion of the portal vein produced reduction of the myoeletric activity and elevation of the percentage frequency of hemorrhage in lamina propria, and a fall of cvp, with relative
Immunohistochemical analysis for CD21, CD35, Caldesmon and S100 protein on dendritic cells types in oral lymphomas
Mesquita, Ricardo Alves;Araújo, Vera Cavalcanti de;Paes, Roberto Antnio Pinto;Nunes, Fábio Daumas;Souza, Suzana Cantanhede Orsini Machado de;
Journal of Applied Oral Science , 2009, DOI: 10.1590/S1678-77572009000300023
Abstract: objective: follicular dendritic cells (fdcs) and interdigitating dendritic cells (idcs) are dendritic cells found in lymphoid follicles, reactive follicles and in lymphomas. the goal of this study was to evaluate the presence and distribution of fdcs and idcs in oral lymphomas. material and methods: immunohistochemistry reactions were applied to 50 oral lymphomas using the antibodies anti-cd21, anti-cd35 and anti-caldesmon to fdcs, and anti-s100 protein to idcs. caldesmon+/fdcs and s100+/idcs were quantified in imagelab? software. results: fdcs revealed by cd21 and cd35 were positively stained in two cases of diffuse large b-cell lymphoma, one malt lymphoma, and in one case of mantle cell lymphoma. fdcs were immunopositive to caldesmon in all cases, as well as idcs to s100 protein. burkitt lymphoma presented a lower amount of caldesmon+/fdcs and s100+/idcs than diffuse large b-cell lymphoma and plasmablastic lymphoma of the oral mucosa type. conclusions: the microenvironment determined by neoplastic lymphoid cells in oral lymphomas is responsible by the development and expression of dendritic cells types.
Descolamento Cr?nico da Placenta: Relato de Caso
Couto, Júlio César Faria;Leite, Juliana Moysés Barbosa;Lages, Cláudia Araújo de Souza Le?o;Machado, Antnio Vieira;Oliveira, Sinval Ferreira de;
Revista Brasileira de Ginecologia e Obstetrícia , 2002, DOI: 10.1590/S0100-72032002000300009
Abstract: chronic placental abruption is a rare condition that can be early detected by ultrasound. vaginal bleeding and uterine excitability can be present in an infrequent way. chronic placental abruption physiopathology is unknown and there are no consistent medical risks that predispose to this condition. the perinatal outcome is poor and is often associated with prematurity and fetal or perinatal death. the obstetric treatment depends on the gestational age, fetal conditions and the size of the clot. we present a case of a chronic placental abruption diagnosed in a 14-week gestation complicated by intrauterine growth retardation, oligohydramnios and perinatal death.
Linfomas em pacientes com a forma hepatoesplênica da esquistossomose mans?nica
Ferraz, álvaro Antnio Bandeira;Sá, Vladimir Curvêlo Tavares de;Lopes, Edmundo Pessoa de Almeida;Araújo Jr, José Guido Corrêa de;Martins, Antnio Cavalcanti de Albuquerque;Ferraz, Edmundo Machado;
Arquivos de Gastroenterologia , 2006, DOI: 10.1590/S0004-28032006000200005
Abstract: background: correlation between infectious agents and linfoproliferative diseases are more stablished, over all virus and bacteria, through the activation of linfocytes. aim: to describe six new cases, of a series of 254 patients (2,36%) with mansonic schistosomiasis, in the hepatosplenic form. methods: six patients will be described, amongst the 254 carriers of mansonic schistosomiasis, in the hepatosplenic form, followed in the last 13 years. results: all the six cases had occurred in women. the histopathologic examinations had evidenced two cases of marginal splenic zone lymphomas, one of great cells with immunoblasts lymphomas, one diffuse lymphomas, a great cells malignant lymphomas, a great not clivads cells, and another case of hodgkin. half of the six evolved for the death 4-15 months after the diagnosis. the others three persist in accompaniment in the oncology division of the clinics hospital. conclusion: the incidence of lymphomas in the 254 mansonic schistosomiasis patients followed in our clinic was of 2,36%. at last, this article intends to call the attention, for the occurrence of lymphomas, in the spleen of patients with mansonic schistosomiasis, in the hepatosplenic form.
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