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Search Results: 1 - 10 of 711 matches for " Deirdre Wall "
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Disentangling the Threads: Analysing Synchronous Online Discussions  [PDF]
Deirdre Grogan
Creative Education (CE) , 2015, DOI: 10.4236/ce.2015.63032
Abstract: This paper investigates features of synchronous chat-communication within a postgraduate e-learning programme that is rooted communities of enquiry (Lave & Wenger, 1991). This project, funded by the Scottish Government, was to provide staff development for teachers throughout Scotland. The paper considers approaches to making sense of disparate threads of online chats between tutors and students and methodological issues in identifying the appropriate unit of analysis. The framework for analysis was derived from promotion of a community of enquiry perspective (Garrison, 2007; Garrison & Vaughan, 2008) which emphasises the importance of the intersection of social, cognitive and teaching presence for achieving shared learning goals. A quantitative content analysis of transcripts from tutor led group chats was used to determine frequencies of teaching presence, social presence, and cognitive presence indicators. Results show that overall, 70% of the contributions were coded as facilitating cognitive discourse. This finding builds on previous research by Ortiz-Rodriguez et al. (2005) which suggests that instructors can influence cognitive presence within synchronous chat. It is suggested that the community of learning enquiry has the potential to help identify critical points for encouraging cognitive presence within synchronous chats. In addition, the study has implications for the growing number of guidelines for the design of online learning environments, particularly in terms of the extent to which synchronous chats have potentially underestimated the value of synchronous learning environments in supporting deeper, critical thinking and shared reflection among learners.
Identification and Validation of Oncologic miRNA Biomarkers for Luminal A-like Breast Cancer
Ailbhe M. McDermott, Nicola Miller, Deirdre Wall, Lorcan M. Martyn, Graham Ball, Karl J. Sweeney, Michael J. Kerin
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0087032
Abstract: Introduction Breast cancer is a common disease with distinct tumor subtypes phenotypically characterized by ER and HER2/neu receptor status. MiRNAs play regulatory roles in tumor initiation and progression, and altered miRNA expression has been demonstrated in a variety of cancer states presenting the potential for exploitation as cancer biomarkers. Blood provides an excellent medium for biomarker discovery. This study investigated systemic miRNAs differentially expressed in Luminal A-like (ER+PR+HER2/neu-) breast cancer and their effectiveness as oncologic biomarkers in the clinical setting. Methods Blood samples were prospectively collected from patients with Luminal A-like breast cancer (n = 54) and controls (n = 56). RNA was extracted, reverse transcribed and subjected to microarray analysis (n = 10 Luminal A-like; n = 10 Control). Differentially expressed miRNAs were identified by artificial neural network (ANN) data-mining algorithms. Expression of specific miRNAs was validated by RQ-PCR (n = 44 Luminal A; n = 46 Control) and potential relationships between circulating miRNA levels and clinicopathological features of breast cancer were investigated. Results Microarray analysis identified 76 differentially expressed miRNAs. ANN revealed 10 miRNAs for further analysis (miR-19b, miR-29a, miR-93, miR-181a, miR-182, miR-223, miR-301a, miR-423-5p, miR-486-5 and miR-652). The biomarker potential of 4 miRNAs (miR-29a, miR-181a, miR-223 and miR-652) was confirmed by RQ-PCR, with significantly reduced expression in blood of women with Luminal A-like breast tumors compared to healthy controls (p = 0.001, 0.004, 0.009 and 0.004 respectively). Binary logistic regression confirmed that combination of 3 of these miRNAs (miR-29a, miR-181a and miR-652) could reliably differentiate between cancers and controls with an AUC of 0.80. Conclusion This study provides insight into the underlying molecular portrait of Luminal A-like breast cancer subtype. From an initial 76 miRNAs, 4 were validated with altered expression in the blood of women with Luminal A-like breast cancer. The expression profiles of these 3 miRNAs, in combination with mammography, has potential to facilitate accurate subtype-specific breast tumor detection.
Exploring Creative Environments through the Child’s Lens  [PDF]
Deirdre Grogan, Joan Martlew
Creative Education (CE) , 2014, DOI: 10.4236/ce.2014.516170
Abstract: There is strong governmental support within the UK to develop creativity and emphasise the importance of its role in learning and teaching. Sharp (2003) identified issues, gaps and priorities for further research that looked at the impact on children of working with professional artists in terms of their creativity. This paper explores the initial findings of an evaluation report conducted by a university research team, exploring the creative performances designed by Starcatchers , an organisation developing performing arts experiences for children aged birth to 4 years. The research team consisted of four action researchers who were each attached to an artist in residence working in four theatre venues across Scotland. The artists involved represented four different art domains: puppetry, visual arts, artistic experiences informed by playing therapy and installation work. The researchers collaborated with the artists to observe children’s engagement, provide feedback, discuss projects, and record the processes of project development. This paper seeks to explore the artists’ experience of designing and implementing participative performance events and the nature and processes of working with young children in performing arts. It focuses on an exploration of the creative learning processes which were developed by the artists in residence to promote children’s creativity and involvement in the visual arts. The role of the artist is examined and key aspects are suggested with a view to enhancing the creative learning experiences provided for children within educational contexts, indicating points for consideration by adults charged with the responsibility of planning and developing environments which support young children’s creativity.
Relationship between Circulating and Tissue microRNAs in a Murine Model of Breast Cancer
Peadar S. Waters, Ailbhe M. McDermott, Deirdre Wall, Helen M. Heneghan, Nicola Miller, John Newell, Michael J. Kerin, Roisin M. Dwyer
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0050459
Abstract: MiRNAs are key regulators of tumorigenesis that are aberrantly expressed in the circulation and tissue of patients with cancer. The aim of this study was to determine whether miRNA dysregulation in the circulation reflected similar changes in tumour tissue. Athymic nude mice (n = 20) received either a mammary fat pad (n = 8, MFP), or subcutaneous (n = 7, SC) injection of MDA-MB-231 cells. Controls received no tumour cells (n = 5). Tumour volume was monitored weekly and blood sampling performed at weeks 1, 3 and 6 following tumour induction (total n = 60). Animals were sacrificed at week 6 and tumour tissue (n = 15), lungs (n = 20) and enlarged lymph nodes (n = 3) harvested. MicroRNAs were extracted from all samples (n = 98) and relative expression quantified using RQ-PCR. MiR-221 expression was significantly increased in tumour compared to healthy tissue (p<0.001). MiR-10b expression was significantly higher in MFP compared to SC tumours (p<0.05), with the highest levels detected in diseased lymph nodes (p<0.05). MiR-10b was undetectable in the circulation, with no significant change in circulating miR-221 expression detected during disease progression. MiR-195 and miR-497 were significantly decreased in tumour tissue (p<0.05), and also in the circulation of animals 3 weeks following tumour induction (p<0.05). At both tissue and circulating level, a positive correlation was observed between miR-497 and miR-195 (r = 0.61, p<0.001; r = 0.41, p<0.01 respectively). This study highlights the distinct roles of miRNAs in circulation and tissue. It also implicates miRNAs in disease dissemination and progression, which may be important in systemic therapy and biomarker development.
miR-379 Regulates Cyclin B1 Expression and Is Decreased in Breast Cancer
Sonja Khan, Cathy L. Brougham, James Ryan, Arisha Sahrudin, Gregory O’Neill, Deirdre Wall, Catherine Curran, John Newell, Michael J. Kerin, Roisin M. Dwyer
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0068753
Abstract: MicroRNAs are small non-coding RNA molecules that control gene expression post-transcriptionally, and are known to be altered in many diseases including breast cancer. The aim of this study was to determine the relevance of miR-379 in breast cancer. miR-379 expression was quantified in clinical samples including tissues from breast cancer patients (n=103), healthy controls (n=30) and patients with benign breast disease (n=35). The level of miR-379 and its putative target Cyclin B1 were investigated on all breast tissue specimens by RQ-PCR. Potential relationships with gene expression and patient clinicopathological details were also determined. The effect of miR-379 on Cyclin B1 protein expression and function was investigated using western blot, immunohistochemistry and proliferation assays respectively. Finally, the levels of circulating miR-379 were determined in whole blood from patients with breast cancer (n=40) and healthy controls (n=34). The level of miR-379 expression was significantly decreased in breast cancer (Mean(SEM) 1.9 (0.09) Log10 Relative Quantity (RQ)) compared to normal breast tissues (2.6 (0.16) Log10 RQ, p<0.01). miR-379 was also found to decrease significantly with increasing tumour stage. A significant negative correlation was determined between miR-379 and Cyclin B1 (r=-0.31, p<0.001). Functional assays revealed reduced proliferation (p<0.05) and decreased Cyclin B1 protein levels following transfection of breast cancer cells with miR-379. Circulating miR-379 was not significantly dysregulated in patients with breast cancer compared to healthy controls (p=0.42). This data presents miR-379 as a novel regulator of Cyclin B1 expression, with significant loss of the miRNA observed in breast tumours.
Prognostic Significance of Deregulated Dicer Expression in Breast Cancer
Emer Caffrey, Helen Ingoldsby, Deirdre Wall, Mark Webber, Kate Dinneen, Laura S. Murillo, Celine Inderhaug, John Newell, Sanjeev Gupta, Grace Callagy
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0083724
Abstract: Background Dicer, an RNase III-type endonuclease, is the key enzyme involved in RNA interference and microRNA pathways. Aberrant expression of Dicer is reported in several human cancers. Our aim was to assess the prognostic role of Dicer in breast cancer. Methods The entire series comprised 666 invasive breast cancers (IBCs), 480 DCIS cases (397 associated with IBC and 83 pure DCIS) and 305 lymph node metastases. Cytoplasmic Dicer expression by immunohistochemistry was scored as negative (no staining) and positive (weak, moderate or strong staining). Results Dicer staining was assessable in 446 IBC, 128 DCIS and 101 lymph node metastases. Expression of Dicer was observed in 33% (145/446) of IBCs, 34% (44/128) of DCIS and 57% (58/101) of lymph node metastases. Dicer expression was increased in nodal metastases compared to primary tumours (p<0.001); and was associated with ER negativity (p<0.001), HER2 positivity (p<0.001), high Ki67 labeling index (p<0.001) and expression of basal-like biomarkers (p = 0.002). Dicer positivity was more frequent in the HER2 overexpressing (p<0.001) and basal-like (p = 0.002) subtypes compared to luminal A subtype. Dicer expression was associated with reduced overall survival (OS) on univariate analysis (p = 0.058) and remained an independent predictor of OS on multivariate analysis (HR 2.84, 95% CI 1.43–5.62, p = 0.003), with nodal status (HR 2.61, 95% CI 1.18–5.80, p = 0.018) and PR (HR 0.28, 95% CI 0.13–0.59, p = 0.001). Further, moderate or strong expression of Dicer was associated with improved disease-free survival in the HER2-overexpressing subtype compared to negative or weak expression (p = 0.038). Conclusion Deregulated Dicer expression is associated with aggressive tumour characteristics and is an independent prognostic factor for OS. Our findings suggest that Dicer is an important prognostic marker in breast cancer and that its prognostic role may be subtype specific.
Impact of Tumour Epithelial Subtype on Circulating microRNAs in Breast Cancer Patients
Peadar S. Waters, Roisin M. Dwyer, Cathy Brougham, Claire L. Glynn, Deirdre Wall, Peter Hyland, Maria Duignan, Mark McLoughlin, John Newell, Michael J. Kerin
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0090605
Abstract: While a range of miRNAs have been shown to be dysregulated in the circulation of patients with breast cancer, little is known about the relationship between circulating levels and tumour characteristics. The aim of this study was to analyse alterations in circulating miRNA expression during tumour progression in a murine model of breast cancer, and to detemine the clinical relevance of identified miRNAs at both tissue and circulating level in patient samples. Athymic nude mice received a subcutaneous or mammary fat pad injection of MDA-MB-231 cells. Blood sampling was performed at weeks 1, 3 and 6 following tumour induction, and microRNA extracted. MicroRNA microArray analysis was performed comparing samples harvested at week 1 to those collected at week 6 from the same animals. Significantly altered miRNAs were validated across all murine samples by RQ-PCR (n = 45). Three miRNAs of interest were then quantified in the circulation(n = 166) and tissue (n = 100) of breast cancer patients and healthy control individuals. MicroArray-based analysis of murine blood samples revealed levels of 77 circulating microRNAs to be changed during disease progression, with 44 demonstrating changes >2-fold. Validation across all samples revealed miR-138 to be significantly elevated in the circulation of animals during disease development, with miR-191 and miR-106a levels significantly decreased. Analysis of patient tissue and blood samples revealed miR-138 to be significantly up-regulated in the circulation of patients with breast cancer, with no change observed in the tissue setting. While not significantly changed overall in breast cancer patients compared to controls, circulating miR-106a and miR-191 were significantly decreased in patients with basal breast cancer. In tissue, both miRNAs were significantly elevated in breast cancer compared to normal breast tissue. The data demonstrates an impact of tumour epithelial subtype on circulating levels of miRNAs, and highlights divergent miRNA profiles between tissue and blood samples from breast cancer patients.
Plural identities among youth of immigrant backround in Montreal
Meintel, Deirdre;
Horizontes Antropológicos , 2000, DOI: 10.1590/S0104-71832000001400002
Abstract: our research concerns the ethnic identity of youth (18-22yrs) of immigrant parentage (greek, portuguese, chilean, vietnamese and salvadoran, with a comparison group of french-speaking québécois youth) in montreal. despite the questioning of notions of ethnicity and ethnic identity that has been going on for several decades, this area of research is still marked by essentialist perspectives that do not coincide with the results of our research. the young people interviewed express fluid ethnic identities that have changed over time and that are characterised by multiple forms of ethnic belonging. they present their ethnic identity as a source of enrichment rather than of conflict or feelings of inferiority. the oral accounts of youth of immigrant origin in montreal give much evidence of transnationality, that is, ties with the cultural group of origin whether in the country of origin or elsewhere; and of transethnicity, solidarities with other ethnic groups in montreal that are seen as culturally or structurally similar. though these two orientations would seem to be somewhat contradictory, they are, we argue, in fact compatible. both offer numerous advantages to immigrant groups and their members, particularly in the present-day context. we examine our research results in light of contextual factors related to the montreal milieu as well as to the age group of those interviewed. we propose that, without minimising these factors, one must question the conceptualisation of ethnic identity especially as it concerns youth of immigrant origins.
Witch and Priest Juxtaposed: Two Figures from Irish Traditional
Deirdre Nuttall
Folklore : Electronic Journal of Folklore , 1998,
Abstract:
Review of 'Technology, Culture, Family: Influences on Home Life'. Author: Elizabeth B. Silva
Deirdre Hynes
International Journal of Gender, Science and Technology , 2011,
Abstract:
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