oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 217 )

2018 ( 322 )

2017 ( 310 )

2016 ( 420 )

Custom range...

Search Results: 1 - 10 of 237238 matches for " Daniela C.;Gregório "
All listed articles are free for downloading (OA Articles)
Page 1 /237238
Display every page Item
Morphological aspects of cluster formation in the germarium of the sugarcane borer Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae)
Santos, Daniela C.;Gregório, Elisa A.;
Neotropical Entomology , 2006, DOI: 10.1590/S1519-566X2006000500012
Abstract: diatraea saccharalis f. is one of the greatest pests of the sugar cane culture. this report aimed to characterize the germarium region of the sugarcane borer by light and transmission electron microscopy, emphasizing the morphological steps of the ovarian cluster formation. in the germarium of this insect, four zones could be morphologically identified during the cluster formation. in the most apical end of each ovariole - zone i - the germ line stem cells undergo complete mitotic division, originating the cystoblasts. in the zone ii, each cystoblast produces a group of eight cells, the cystocytes, which are interconnected by the ring canals. clusters containing all the cystocytes in the meiosis, characterizes the zone iii. germ cells with ultrastructural features of apoptosis are also detected in this zone. in the zone iv the cystocytes differentiate, morphologically, into one oocyte and seven nurse cells. interstitial somatic cells and pre-follicle cells exhibit, in their cytoplasm, heterogeneous vacuoles containing degenerated cellular fragments, characterized as apoptotic bodies. our results pointed out to the morphological evidences related with important control mechanisms for new clusters/follicles production and for the cellular arrangement into the germarium, resulting from the programmed cell death. we believe that the morphological characterization of ovarian cluster formation in d. saccharalis provided valuable information for the understanding of the initial steps of oogenesis and contributed for the knowledge of the cellular mechanisms related with the oocyte production and with reproduction in insects.
Histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae)
Victoriano, Eliane;Pinheiro, Daniela O.;Gregório, Elisa A.;
Neotropical Entomology , 2007, DOI: 10.1590/S1519-566X2007000500011
Abstract: the silk gland in lepidoptera larvae is responsible for the silk production used for shelter or cocoon construction. the secretion of fibroin and sericin by the different silk gland regions are well established. there are few attempts to detect lipid components in the insect silk secretion, although the presence of such element may contribute to the resistance of the shelter to wet environment. this study characterizes the glandular region and detects the presence of lipid components in the secretion of the silk gland of diatraea saccharalis(fabricius). the silk gland was submitted to histochemical procedure for lipid detection or conventionally prepared for ultrastructural analyses. lipid droplets were histochemically detected in both the apical cytoplasm of cell of the anterior region and in the lumen among the microvilli. ultrastructural analyses of the anterior region showed lipid material, visualized as myelin-like structures within the vesicular golgi complex and in the apical secretory globules, mixed up with the sericin; similar material was observed into the lumen, adjacent to the microvilli. lipids were not detected in the cells neither in the lumen of the posterior region. our results suggest that the silk produced by d. saccharalis has a minor lipid content that is secreted by the anterior region together with the sericin.
Um caso de síndrome de compress o do tronco celiaco com eventual componente iatrogénico
N. Almeida,P. Amaro,C. Gon?alves,C. Gregório
Jornal Português de Gastrenterologia , 2006,
Abstract: Apresenta-se o caso de um doente de 64 anos com epigastralgias, náuseas e vómitos recorrentes, associados a astenia, anorexia e emagrecimento significativo nos últimos meses. Estas queixas estavam presentes desde 1998 mas agravaram-se em 2002 após cirurgia para correc o de acalásia. Perante a avalia o efectuada colocaram-se como hipóteses diagnósticas uma pancreatite aguda idiopática recorrente ou uma isquémia mesentérica. A realiza o de arteriografia abdominal evidenciou uma estenose significativa do tronco celíaco, compatível com síndrome de compress o do tronco celíaco. A revis o do processo radiológico sugeriu eventual sequela de lobectomia superior esquerda realizada em 1994. A 64 years old man presented with recurrent epigastric pain, nausea and vomiting associated with asthenia, anorexia and significant weight loss in the last months. These complaints began in 1998 but worsen in 2002, after surgery for achalasia. The evaluation performed so far favored the diagnosis of recurrent idiopathic acute pancreatitis or a mesenteric ischemia. Abdominal angiography performed revealed a significant celiac trunk stenosis compatible with celiac trunk compression syndrome. Reevaluation of the radiological file suggested a sequel of left superior lobectomy performed in 1994.
O bal o intragástrico nas formas graves de obesidade
N. Almeida,D. Gomes,C. Gon?alves,C. Gregório
Jornal Português de Gastrenterologia , 2006,
Abstract: Introdu o: Nos doentes com obesidade mórbida o bal o intragástrico (BIG) pode ser uma ponte para a cirurgia ou uma terapêutica temporária nos n o candidatos à mesma. Objectivo: Avaliar a eficácia do BIG em doentes com obesidade severa. Doentes e Métodos: Em 2003/2004 foram colocados 17 BIG Bioenterics preenchidos com soro fisiológico e azul-demetileno [volume médio-508,8 ml (450-600)] em 17 doentes [11 mulheres; média etária-49,2 (27-69 anos); índice de Massa Corporal médio-55,6 (40,2-74,2 Kg/m2)], acompanhados previamente em consultas de Nutri o e/ou Endocrinologia. Já tinham sido submetidos a medidas higieno-dietéticas e/ou farmacológicas, com sucesso limitado. Observadas co-morbilidades em 13 (76,5%). Resultados: Oito (47%) doentes apresentaram náuseas/vómitos nas 24-72h seguintes situa o que se manteve em 4 (23,5%), condicionando desidrata o e insuficiência renal aguda pré-renal, obrigando à remo o precoce do bal o (0,5 a 4 meses). Nos restantes doentes o BIG foi removido aos 6 meses (num aos 10). Todos os doentes perderam peso (5-70 Kg); perda média-19,6 Kg (p<0,001). Sem casos de rotura espontanea. Seis (35,3%) foram posteriormente operados. Conclus es: O BIG é um método eficaz na redu o de peso em indivíduos com obesidade mórbida. As náuseas e os vómitos s o as complica es mais comuns. Embora desejável nem sempre se consegue a transposi o para a cirurgia. Introduction: In patients with morbid obesity the intragastric balloon (IGB) can be a "bridge" to surgery or a temporary treatment in patients who are not candidates for surgery. Objective: Evaluate IGB efficacy in morbidly obese patients. Patients and Methods: In 2003/2004 seventeen IGB Bioenterics filled with normal saline and methylene blue were placed in 17 patients [11 women, median age was 49.2 (27-69 years); median body mass index was 55.6 (40.2-74.2 Kg/m2)], followed by nutritionists and/or endocrinologists. They had previously tried dietetic and/or pharmacological measures with limited results. Co-morbidities were present in 13 (76.5%). Results: Eight (47%) patients presented nausea/vomiting in the first 24-72h that persisted in 4 (23.5%) leading to dehydration and pre-renal insufficiency and forcing premature removal of the balloon (0.5 to 4 months). In the other patients, the device was removed at 6 months treatment (in 1 patient at 10 months). All patients suffered weight loss (5-70 Kg); median loss-19.6 Kg (p<0.001). No cases of spontaneous deflation/displacement occurred. Six (35.3%) underwent bariatric surgery. Conclusions: The IGB is a useful method for weight loss in
Injec o intralesional de betametasona nas estenoses benignas do esófago
C. Gon?alves,N. Almeida,D. Gomes,C. Gregório
Jornal Português de Gastrenterologia , 2006,
Abstract: A injec o intralesional de corticoides tem sido usada nas estenoses benignas do esófago com vista a potenciar os efeitos da dilata o endoscópica. Na maioria dos estudos tem sido utilizada a triancinolona, sendo poucos os trabalhos publicados com a betametasona. Objectivos: Avaliar a eficácia e seguran a da associa o de dilata o e injec o intralesional de betametasona nas estenoses benignas do esófago. Métodos: Análise retrospectiva dos relatórios dos exames efectuados entre 1 de Janeiro de 1998 e 31 de Dezembro de 2004, seleccionando os casos de estenose benigna esofágica nos quais foi usada a associa o dilata o/injec o intralesional de betametasona. Identificámos 31 doentes (21 homens e 10 mulheres), com média etária de 58,8 anos. A etiologia das estenoses foi mais frequentemente pós-ciurgica (16 doentes), seguida das estenoses pépticas (11casos) e cáusticas (5 casos). Resultados: Houve uma redu o estatisticamente significativa do índice de dilata o médio "pré-injec o" e "pósinjec o" (p=0,002), representando uma diminui o de cerca de 1,35 dilata es/mês após a terapêutica. N o se verificou aumento significativo do diametro luminal médio antes e após a injec o intralesional. No nosso estudo n o encontrámos registos de quaisquer eventos adversos ocorridos na sequência da terapêutica. Conclus o: A injec o intralesional de betametasona é uma técnica segura e eficaz. No entanto, os nossos resultados n o permitem tirar conclus es dado o pequeno número de doentes estudados. Intralesional steroid injection has been used in benign oesophageal strictures to improve the results of dilatation. Most studies use triamcinolone, only a few have reported using betamethasone. Objectives: To evaluate the efficacy and safety of the combination of dilatation and intralesional betamethasone injection in benign oesophageal strictures. Methods: Cases of benign oesophageal strictures treated with dilatation and betamethasone injection between January 1st 1998 and December 31st 2004 were retrospectively analysed. Thirty-one patients (21 males, 10 females) were identified, with a mean age of 58.8 years. The most frequent aetiologies of the stenosis were post-surgical (16 patients), peptic (11 cases) and caustic (5 cases). Results: The periodic dilatation index significantly decreased (p=0.002), although there was no statistically significant variation between medium luminal diameter pre and post-treatment. There were no adverse events. Conclusion: Intralesional betamethasone injection is a safe technique which increases efficacy of endoscopic dilatation. However,
Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae
Pinheiro, Daniela O.;Quagio-Grassiotto, Irani;Gregório, Elisa A.;
Neotropical Entomology , 2008, DOI: 10.1590/S1519-566X2008000400009
Abstract: the sugarcane borer, diatraea saccharalis fabricius, is a pest to sugarcane and many other crops. this work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of d. saccharalis larvae. fragments of the midgut (anterior, middle and posterior regions) were fixed and processed by light and scanning electron microscopy. there are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. the apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions. there is an increase in the amount of pas-positive (periodic acid-schiff reaction) granules detected in the cytoplasm of both the columnar and regenerative cells, from the anterior to the posterior region. the goblet cell apical surface is narrow in the anterior region, and enlarged in the posterior midgut; the chamber's cytoplasm extrusion are small and thin at the apical cavity surface, being thicker, longer and more numerous at the basal portion of the cavity. our results suggest that the sugarcane borer midgut has two morphologically different regions, the anterior and the posterior; the middle region is a transitional region.
Morphometric study of the midgut epithelium in larvae of Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae)
Pinheiro, Daniela O.;Silva, Reinaldo J.;Quagio-Grassiotto, Irani;Gregório, Elisa A.;
Neotropical Entomology , 2003, DOI: 10.1590/S1519-566X2003000300012
Abstract: the sugarcane borer, diatraea saccharalis fabricius, has great economical interest as it affects the culture and industrial use of the sugarcane. however, there are few studies concerning the internal morphology of this insect. this work aims to study morphometrically the midgut and the epithelium along their lenght, trying to characterize different regions. midgut of last instar larvae was divided in three regions: anterior, middle and posterior, and the fragments were processed for light microscopic observation. histological sections were analyzed in a computerized system concerning the length, width and area of the epithelium, their cells, and the midgut lumen. the obtained data were statistically analyzed by the kruskal-wallis test and by multivariate analysis. our results showed that the midgut has two different regions, the anterior and the posterior; the middle region presents values that are coincident with the ones of either the anterior and the posterior portions, suggesting that there is an intermediate region between the other two ones. the epithelial cells (columnar, goblet and regenerative cells), when evaluated by multivariate analysis, do not present significant morphometric differences in the different midgut regions. however, the analysis of variance for separate variables show that the regenerative cells present wide morphometric variability along the midgut.
Spherites in the midgut epithelial cells of the sugarcane borer parasitized by Cotesia flavipes
de Oliveira Pinheiro,Daniela; Conte,Hélio; Aparecida Gregório,Elisa;
Biocell , 2008,
Abstract: diatraea saccharalis, the main pest of sugarcane, has been controlled by cotesia flavipes. very little is known about the effect of parasitism on the host organs, including the midgut. the lepidoptera midgut epithelium is composed of columnar, goblet, regenerative, and endocrine cells. spherites have been described in columnar and regenerative cells of several lepidoptera species, and presented a lot of functional meaning. we identified spherites in the midgut epithelial cells of non-parasitized d. saccharalis larvae analyzed the effect of parasitism on spherite morphology and distribution along the length of the midgut. midgut fragments of both non-parasitized and parasitized larvae were processed for transmission electron microscopy. all the midgut epithelial cells showed spherites, but they were not preferentially located in a particular part of the cells. parasitized larvae had more spherites, mainly in the columnar cells, than non-parasitized larvae. this observation was associated with an ionic imbalance within the insect host. spherites were more abundant in the anterior midgut region than in other regions, which suggests that this region is involved in ion transport by intracellular and/or paracellular route. the morphological variability of spherites in the cells of parasitized larvae was related to the developmental stages of these structures.
Spherites in the midgut epithelial cells of the sugarcane borer parasitized by Cotesia flavipes
Daniela de Oliveira Pinheiro,Hélio Conte,Elisa Aparecida Gregório
Biocell , 2008,
Abstract: Diatraea saccharalis, the main pest of sugarcane, has been controlled by Cotesia flavipes. Very little is known about the effect of parasitism on the host organs, including the midgut. The Lepidoptera midgut epithelium is composed of columnar, goblet, regenerative, and endocrine cells. Spherites have been described in columnar and regenerative cells of several Lepidoptera species, and presented a lot of functional meaning. We identified spherites in the midgut epithelial cells of non-parasitized D. saccharalis larvae analyzed the effect of parasitism on spherite morphology and distribution along the length of the midgut. Midgut fragments of both non-parasitized and parasitized larvae were processed for transmission electron microscopy. All the midgut epithelial cells showed spherites, but they were not preferentially located in a particular part of the cells. Parasitized larvae had more spherites, mainly in the columnar cells, than non-parasitized larvae. This observation was associated with an ionic imbalance within the insect host. Spherites were more abundant in the anterior midgut region than in other regions, which suggests that this region is involved in ion transport by intracellular and/or paracellular route. The morphological variability of spherites in the cells of parasitized larvae was related to the developmental stages of these structures.
Successful endoscopic bandig after cyanoacrylate failure for active bleeding duodenal varix Ligadura endoscópica eficaz tras el fracaso del cianoacrilato en la hemorragia activa de una variz duodenal
H. T. Sousa,C. Gregório,P. Amaro,M. Ferreira
Revista Espa?ola de Enfermedades Digestivas , 2008,
Abstract:
Page 1 /237238
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.