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Search Results: 1 - 10 of 181322 matches for " Damares de Castro;Giglioti "
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Brazilian coffee genome project: an EST-based genomic resource
Vieira, Luiz Gonzaga Esteves;Andrade, Alan Carvalho;Colombo, Carlos Augusto;Moraes, Ana Heloneida de Araújo;Metha, ?ngela;Oliveira, Angélica Carvalho de;Labate, Carlos Alberto;Marino, Celso Luis;Monteiro-Vitorello, Claúdia de Barros;Monte, Damares de Castro;Giglioti, éder;Kimura, Edna Teruko;Romano, Eduardo;Kuramae, Eiko Eurya;Lemos, Eliana Gertrudes Macedo;Almeida, Elionor Rita Pereira de;Jorge, érika C.;Albuquerque, érika V. S.;Silva, Felipe Rodrigues da;Vinecky, Felipe;Sawazaki, Haiko Enok;Dorry, Hamza Fahmi A.;Carrer, Helaine;Abreu, Ilka Nacif;Batista, Jo?o A. N.;Teixeira, Jo?o Batista;Kitajima, Jo?o Paulo;Xavier, Karem Guimar?es;Lima, Liziane Maria de;Camargo, Luis Eduardo Aranha de;Pereira, Luiz Filipe Protasio;Coutinho, Luiz Lehmann;Lemos, Manoel Victor Franco;Romano, Marcelo Ribeiro;Machado, Marcos Antonio;Costa, Marcos Mota do Carmo;Sá, Maria Fátima Grossi de;Goldman, Maria Helena S.;Ferro, Maria Inês T.;Tinoco, Maria Laine Penha;Oliveira, Mariana C.;Van Sluys, Marie-Anne;Shimizu, Milton Massao;Maluf, Mirian Perez;Eira, Mirian Therezinha Souza da;Guerreiro Filho, Oliveiro;Arruda, Paulo;Mazzafera, Paulo;Mariani, Pilar Drummond Sampaio Correa;Oliveira, Regina L.B.C. de;Harakava, Ricardo;Balbao, Silvia Filippi;Tsai, Siu Mui;Mauro, Sonia Marli Zingaretti di;Santos, Suzana Neiva;Siqueira, Walter José;Costa, Gustavo Gilson Lacerda;Formighieri, Eduardo Fernandes;Carazzolle, Marcelo Falsarella;Pereira, Gon?alo Amarante Guimar?es;
Brazilian Journal of Plant Physiology , 2006, DOI: 10.1590/S1677-04202006000100008
Abstract: coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. the genus coffea belongs to the rubiaceae family which includes other important plants. the genus contains about 100 species but commercial production is based only on two species, coffea arabica and coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. the brazilian coffee genome project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. we have single-pass sequenced a total of 214,964 randomly picked clones from 37 cdna libraries of c. arabica, c. canephora and c. racemosa, representing specific stages of cells and plant development that after trimming resulted in 130,792, 12,381 and 10,566 sequences for each species, respectively. the ests clustered into 17,982 clusters and 32,155 singletons. blast analysis of these sequences revealed that 22 % had no significant matches to sequences in the national center for biotechnology information database (of known or unknown function). the generated coffee est database resulted in the identification of close to 33,000 different unigenes. annotated sequencing results have been stored in an online database at http://www.lge.ibi.unicamp.br/cafe. resources developed in this project provide genetic and genomic tools that may hold the key to the sustainability, competitiveness and future viability of the coffee industry in local and international markets.
Rendimiento de boquillas hidráulicas y atomizadores en la aplicación aérea de fungicidas en la ca a de azúcar
Vagner Amado Belo de Oliveira,Gustavo Migliorini de Oliveira,éder Antonio Giglioti,Wagner Teigi Igarashi
Pesquisa Aplicada & Agrotecnologia , 2012, DOI: 10.5777/1554
Abstract: Con la aparición de la epidemia de roya naranja de la hoja de ca a de azúcar (Saccharum officinarum) causada por el hongo Puccinia kuehnii, las aplicaciones de fungicidas se hicieran necesarias en las variedades susceptibles. Sin embargo, pocos estudios relativos a la tecnología de aplicación se encuentran en la literatura, especialmente en relación con la aplicación aérea. El objetivo fue evaluar la distribución de la pulverización de fungicidas con aplicación aérea en cultivo de ca a de azúcar con diferentes boquillas de pulverización. Dos experimentos fueron desarrollados en dise o en factorial completamente al azar con cinco repeticiones. El primer experimento se llevó a cabo en un factorial 2 x 4 con atomizador tipo Micronair, teniendo como factores las tasas de aplicación (10 y 20 l ha-1) y las posiciones en la planta (hoja +1 punta, hoja + 1 inserción de la lígula, hoja + 3 y hoja + 5), respectivamente. El segundo experimento fue estructurado en un factorial 2 x 2 x 4 para la boquilla de pulverización hidráulica asociando los factores ángulo de inclinación del chorro (00 y 450), tasa (30 y 40 L ha-1) y las mismas posiciones del experimento I en la planta. Las aplicaciones del fungicida azoxistrobina + ciproconazol (0,5 L h-1) con la adición de aceite mineral (0,6 L h-1) se hicieron con una aeronave modelo Ipanema. Las variables evaluadas fueron el diámetro medio de volumen (VMD), el porcentaje de cobertura y el número de gotas por cm2. Los resultados mostraron, para las tres variables, que hube similitudes estadísticas entre las boquillas de pulverización en prueba, así como entre las técnicas de aplicación (tasas y ángulos) estudiados. Tampoco se verificó diferencias significativas en las posiciones de las hojas de la planta. En consecuencia, los resultados muestran la posibilidad de utilizar reducción de las tasas de volumen de aplicación y la aplicabilidad de boquillas hidráulicas para este tipo de pulverización. Com o surgimento epidêmico da ferrugem alaranjada da cana-de-a úcar (Saccharum officinarum) causada pelo fungo Puccinia kuehnii, as aplica es de fungicidas tornaram-se necessárias nas variedades susceptíveis. Todavia, poucos estudos concernentes à tecnologia de aplica o s o encontrados na literatura, especialmente em rela o à aplica o aérea. O objetivo foi avaliar a distribui o da calda de fungicida em aplica o aérea para cana-de-a úcar com diferentes bicos de pulveriza o. Desenvolveu-se dois experimentos em esquema fatorial de forma inteiramente casualizada com 5 repeti es. O experimento I realizou-se em um fatorial 2 x 4 para
Detection of Babesia bovis and Babesia bigemina in Water Buffaloes (Bubalus bubalis) in Endemic Areas of S?o Paulo State, Brazil  [PDF]
T. A. Néo, R. Giglioti, D. Obregón, T. B. Bilhassi, H. N. Oliveira, R. Z. Machado, F. de F. Aníbal, L. G. Brito, W. Malagó Jr., F. A. Bressani, M. C. S. Oliveira
Open Journal of Veterinary Medicine (OJVM) , 2016, DOI: 10.4236/ojvm.2016.65009
Abstract: Babesiosis is a tick-transmitted disease that causes severe economic losses to the cattle industry in Brazil. Water buffaloes (Bubalus bubalis) are often carriers of Babesia spp., but there are no studies that provide an accurate estimation of this infection in animals raised in regions of endemic stability. This study was conducted to investigate Babesia bovis and B. bigemina infections in 108 water buffaloes (50 calves and 58 adult females) located in areas of São Paulo state, where the animals were continuously exposed to Rhipicephalus microplus ticks. B. bovis and B. bigemina infections were screened by microscopic examination of blood smears, nested PCR (nPCR) and quantitative real-time PCR (qPCR), which were also used to estimate the number of copies (NC) of the cytochrome b (mt-cytB) gene in the blood samples. B. bigemina was found in blood smears of three calves from Alambari herd (all with less than 0.1% parasitemia). Molecular techniques were more sensitive than blood smears to diagnose piroplasms in water buffaloes: 20.37% and 100.00% for B. bovis-infected animals and 59.26% and 100.00% for B. bigemina-infected animals, respectively for nPCR and qPCR. The NC of mt-cytB gene of B. bovis and B. bigemina in blood samples revealed significant effects (p < 0.05) of herd-age, species and their interaction. The NC values were higher (p ≤ 0.05) for B. bigemina (2.80 ± 0.06) than for B. bovis (2.61 ± 0.05). Within each herd-age, differences between the species’ NC values were found only in Alambari calves, which showed significantly higher (p ≤ 0.05) NC of B. bigemina (3.48 ± 0.13). The calves and cows from Ibaté showed the lowest NC of B. bigemina (2.29 ± 0.13 and 2.63 ± 0.14) and B. bovis (2.54 ± 0.11 and 2.37 ± 0.12), respectively. These data suggest a high prevalence of B. bovis and B. bigemina infection in the buffalo population in endemic areas of São Paulo state.
Trends of Development of International Water Law  [PDF]
Paulo Canelas de Castro
Beijing Law Review (BLR) , 2015, DOI: 10.4236/blr.2015.64025
Abstract: International water law has been witnessing profound changes in the latter twenty years. These changes denote certain cardinal options for new goals and values that the newer international water law is seeking to embrace. Amongst these new goals and values, the ones of environmentalization, humanisation, economicisation stand out as attempts at bringing efficient response to the challenges set by the current global water crisis and the more complex expectations of the international community. They amount to an innovative normative message and even true paradigm-shifts in the understanding of international water law. These trends of development of international water law set this legal field in line with other chapters of contemporary international law and derive in part from the cross-fertilisation of normative ideas and principles between these chapters of international law.
Psychoanalysis, Mental Health and Drug Issues  [PDF]
Edson Olivari de Castro
Psychology (PSYCH) , 2017, DOI: 10.4236/psych.2017.811119
Abstract: This article aims to discuss some of the problems we can encounter in addressing drug issues, especially in Brazil. It also shows, through the work of some authors, that psychoanalysts must go beyond the frontiers of psychoanalysis itself—overcoming elaborations that individualize the problem through concepts such as personality or structure—to enable a new understanding that can guarantee different strategies of intervention. At the end, it points out questions opened from the indicated problems, which are referring to the discourse of capitalism in the present time.
Especificidade de anti-soro policlonal à Leifsonia xyli subsp. xyli
Carneiro Jr, Josil B.;Silveira, Silvaldo F. da;Souza Filho, Gon?alo A. de;Olivares, Fabio L.;Giglioti, éder A.;
Fitopatologia Brasileira , 2004, DOI: 10.1590/S0100-41582004000600003
Abstract: to detect the presence of the bacterium leifsonia xyli subsp. xyli (lxx) in propagation material is important for the control of the ratoon stunting disease. the objective of this work was to improve a serological method for detection of the pathogen and to evaluate the policlonal antiserum specificity produced against lxx. the antigen was prepared by resuspending pure culture in pbs and dialyzing it in glutaraldehyde 2% in pbs. the immunization schedule in rabbit constituted of two intramuscularly injections of 1:1 mixture of the antigen: freund adjuvant (complete and incomplete, with interval of 21 days) and two subcutaneous injections of the antigen, with ten-day intervals. the antiserum was tested by dot blot and revealed by peroxidase to determine: (i) title of the antibody, (ii) reaction and specificity against lxx, xanthomonas axonopodis pv. vesicatoria and endophytic sugarcane (saccharum sp.) bacteria (azospirillum brasilense, a. lipoferum, hebaspirillum rubrisubalbicans, h. seropedicae, gluconacetobacter diazotrophicus). the highest dilution of antiserum (1:20.000) presented strong positive reaction and high specificity against lxx. the purification of the igg fraction did not improve the antiserum reactivity and specificity. in bacterial cell suspension the level of detection was estimated in 2x106 cells/ml.
A eficácia de exercícios para disfun??o da tuba auditiva
Sperancini, Camila Le?o;Souza, Damares Plácido Moreira de;Silva, Tarcimara Moreira;Di Ninno, Camila Queiroz de Moraes Silveira;Amorim, Márcia Nogueira;
Revista da Sociedade Brasileira de Fonoaudiologia , 2007, DOI: 10.1590/S1516-80342007000100008
Abstract: purpose: to assess the effectiveness of blowing, suction, and nasal breathing exercises, associated with nose cleaning and valsalva's maneuver, for eustachian tube dysfunctions and preventing recurrent otitis media. methods: twenty-two children of both genders, with ages between three and six years, who had a history of recurrent otitis media and, at the moment of the exam, types a and c tympanometric curves participated in the study. the subjects were submitted daily to the above-mentioned procedures for 3 months. results: at the end of the study, the last immitance audiometry of the 22 analyzed children showed that 18 of them had bilateral type a curves and no otitis episodes during this interval. conclusion: in the studied sample, these procedures proved to be effective for preventing tube dysfunction and recurrent otitis media, and may be used preventively in children at day care centers.
Efficacy evaluation of a commercial neem cake for control of Haematobia irritans on Nelore cattle
Chagas, Ana Carolina de Souza;Oliveira, Márcia Cristina de Sena;Giglioti, Rodrigo;Calura, Fernando Henrique;Ferrenzini, Jenifer;Forim, Moacir Rossi;Barros, Antonio Thadeu Medeiros de;
Revista Brasileira de Parasitologia Veterinária , 2010, DOI: 10.1590/S1984-29612010000400005
Abstract: much attention has been given to the development of botanical insecticides to provide effective natural control of cattle ectoparasites without harming animals, consumers, and environment. this study evaluated the efficacy of a commercial neem cake in controlling haematobia irritans infestation on cattle. the study was conducted at the embrapa southeast cattle research center (cppse), in s?o carlos, sp, brazil, from april to july 2008. the neem cake mixed in mineral salt in a 2% concentration was provided to 20 nelore cows during nine weeks and had its efficacy evaluated by comparison of the infestation level against a control group. fly infestations were recorded weekly by digital photographs of each animal from both groups and the number of flies was later counted in a computer-assisted image analyzer. quantification of neem cake components by high-performance liquid chromatography revealed the presence of azadirachtin (421 mg.kg-1) and 3-tigloyl-azadirachtol (151 mg.kg-1) in the tested neem cake. addition of the 2% neem cake reduced mineral salt intake in about 22%. the 2% neem cake treatment failed to reduce horn fly infestations on cattle during the 9-week study period.
Analysis of the nucleotide sequence of the coat protein and 3'-untranslated region of two Brazilian Potato virus Y isolates
INOUE-NAGATA, ALICE K.;FONSECA, M. ESTHER N.;LOBO, TATIANA O.T.A.;áVILA, ANT?NIO C. DE;MONTE, DAMARES C.;
Fitopatologia Brasileira , 2001, DOI: 10.1590/S0100-41582001000100008
Abstract: two brazilian potato virus y (pvy) isolates were biologically characterized as necrotic (pvy-nbr) and common (pvy-obr) based upon symptoms on test plants. additional characterization was performed by sequencing a cdna corresponding to the 3' terminal region of the viral genome. the sequence consisted of 195 nucleotides (nt) coding part of the nuclear inclusion body b (nib) gene, 804 nt of the coat protein (cp) gene, and 328 nt (pvy-obr) or 326 nt (pvy-nbr) of the 3'-untranslated region (utr). translation of the sequence resulted in one single open reading frame with part of the nib and a cp of 267 amino acids. the two isolates shared 95.1% similarity in the cp amino acid sequence. the cp and the 3'-utr sequence of the brazilian isolates were compared to those of other pvy isolates previously reported and unrooted phylogenetic trees were constructed. the trees revealed a separation of two distinct clusters, one comprising most of the common strains and the other comprising the necrotic strains. pvy-obr was clustered in the common group and pvy-nbr in the necrotic one.
Differential IL10 mRNA Profiles Associated to Babesia bovis and B. bigemina Infection Levels in Persistently Infected Animals  [PDF]
Talita Barban Bilhassi, Rodrigo Giglioti, Cintia Hiromi Okino, Wilson Malagó Júnior, Henrique Nunes de Oliveira, Cíntia Righetti Marcondes, Márcia Cristina de Sena Oliveira
Open Journal of Veterinary Medicine (OJVM) , 2019, DOI: 10.4236/ojvm.2019.911014
Abstract: This work aimed to find quantitative phenotypic traits that can be used to discriminate the levels of resistance/susceptibility to B. bovis and B. bigemina in two groups of cattle presenting the highest (H) or lowest (L) infection levels and Rhipicephalus microplus ticks count. The animals were selected from a previous study of 50 Canchim (5/8 Charolais/zebu) heifers raised in an endemic area for these parasites. These animals were evaluated regarding their TNFα, IL10, IFN-γ, IL12 and iNOS mRNA levels. No differences were found between these groups regarding TNFα, IFN-γ, IL12β or iNOS transcripts. However, the IL10 transcripts were significantly higher in the H group compared to the L group. Moreover, significant correlation coefficients were observed between B. bovis loads and both IL10 and IFN-γ transcripts, while no correlations were found for B. bigemina loads and all tested immune-related transcripts, suggesting that differential IL10 mRNA profiles were closely associated to B. bovis loads. Our results have contributed to a better understanding of the immune responses against Babesia infection, as we demonstrated that the IL10 cytokine levels might also influence or be influenced by parasitemia levels in persistently infected animals.
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