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Search Results: 1 - 10 of 574845 matches for " D.K. GHOSH and Y.S. AHLAWAT "
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PCR detection of Candidatus Liberibacter asiaticus, the agent of Huanglongbin or greening disease in citrus
V.K. BARANWAL*, S. MAZUMDER, JITENDER SINGH, V. SURYANARAYANA, D.K. GHOSH and Y.S. AHLAWAT
Indian Phytopathology , 2012,
Abstract: The causal agent of Huanglongbing (HLB) or citrus greening disease (CGD) was detected from total DNA isolated from midrib of leaves of citrus trees from Delhi, Jammu and Maharashtra showing symptoms of yellowing or yellow mottling by PCR amplification of ribosomal protein gene. The amplicon was cloned in pGEM-T easy vector and sequenced. The clone was 703 nucleotide long and showed 100 % nucleotide sequence identity with part of b operon gene of Asian species of greening bacterium i.e. Candidatus Liberibacter asiaticus but differed from African species of greening bacterium i.e. Candidatus Liberibacter africanus. Our studies indicated that b operon gene of Asian species of greening bacterium is highly conserved and can be used for quick detection of greening bacterium in citrus plant for phytosanitary and certification programme.
Detection of brinjal little leaf phytoplasma in phloem elementsisolated by enzyme treatment
A.K. DAS1, D.K. MITRA and Y.S. AHLAWAT
Indian Phytopathology , 2012,
Abstract: Phloem elements of little leaf infected aubergine plants were separated from other cell types by partial digestion of plant tissue with macerating enzymes and viewed by fluorescence microscopy after staining with the fluorochrome, 4', 6-diamidino-2-phenylindole (DAPI). The isolated phloem elements from infected plants showed typical fluorescence indicating the presence of the causal phytoplasma. No such specific fluorescence was observed in healthy phloem tissues.
Viroid infection of citrus in Central India
D.K. GHOSH, S. MATHUR, K. N. GUPTA, Y. S. AHLAWAT and P. RAMACHANDRAN
Indian Phytopathology , 2012,
Abstract: A survey of citrus orchards in certain parts of Central India revealed the presence of characteristic symptoms of bark scaling and leaf yellowing on Nagpur mandarin and mosambi grafted on Rangpur lime and rough lemon rootstock. Samples were collected from field and grafted on Etrog citron indicator plants. Nucleic acid extracted from bark tissue of field trees were also ana lysed by return polyacrylamide gel electrophoresis (R-PAGE). Results of both biological and R-PAGE analysis showed viroid infection in citrus orchards in this region.
Novel Genotypes of Type 2 Porcine Circovirus (PCV2) in PMWS Pigs in China Between 2008 and 2009
G.H. Zhao,W. Cheng,P.J. Zhang,Y.S. Han,D.K. Chen
Journal of Animal and Veterinary Advances , 2012, DOI: 10.3923/javaa.2010.3083.3091
Abstract: The present study analyzed genetic variation and genotypes of 17 strains of type 2 Porcine Circovirus (PCV2) from different epidemic regions in China in 2008 and 2009. All the genomic sequences were 1.767 bp in length. Sequence comparison of complete genomic sequences revealed 95.6-99.9% identity among 17 PCV2 strains and the most variable regions within 1.000-1.700 nt (located in the coding region of ORF2). Comparative analysis of amino acids of the two ORFs revealed that variation extend of ORF2 (93.1-100%) was greater than ORF1 (98.4-100%) and the third codon position showed much more variable than the first and second sites. Mutations in T and B lymphocyte epitopes were also detected by comparative analysis and it was found that T lymphocyte epitopes were more conserved than those of B lymphocytes. Phylogenetic analysis revealed 6 novel genotypes of PCV2 in addition to the 5 known geneotypes (PCV-2a, PCV-2b, PCV-2c, PCV-2d, PCV-2e) reported. Of these genotypes, the PCV-2b, PCV-2d and 3 unidentified genotypes were the most prevailing, within 13, 17 and 16 epidemic provinces, respectively. For the 17 Chinese PCV2 strains examined in this study, 5 strains represented PCV-2b genotype, 6 strains were PCV-2d, 1strain was PCV-2e and other 5 strains were novel genotypes while no strains were PCV-2a and PCV-2c genotype. These findings demonstrated the usefulness and attributes of complete genomic sequences for genetic variation and genotyping of PCV2 and have implications for the studies of population biology, molecular epidemiology and genetic structure of PCV2 and for the effective control of PMWS as well.
Mosaic disease in chilli (Capsicum annuum) cv Kalimpong Local in Darjeeling hills of West Bengal and its management
K.K. BISWAS*, K.B. PUN, R.P. PANT and Y.S. AHLAWAT
Indian Phytopathology , 2012,
Abstract: The symptoms of chilli mosaic diseare in the cv Kalimpong Local popularly known as Dolo Kursani in hill regions of Darjeeling District of West Bengal and Sikkim state include green to yellow mosaic, mottling of leaves with or without deformation, stunting and extremely deformed shoe string leaf in advanced stages of the disease. Incidence of the disease ranged from 5- 100% with low incidence of higher altitude. The virus was mechanically transmitted to solanaceous and cruciferous hosts, chilli, tobacco, radish and rye. In electron microscope, negatively stained preparations from naturally infected leaf samples revealed the presence of two types of virus particles, one similar to Cucumber mosaic cucumovirus (CMV) and other similar to Potato virus Y (PVY). Both single and mixed infections of these viruses were confirmed by EM decoration test and DAC-ELISA. Field experiment revealed that planting of crop in the month of March reduces disease incidence thereby increasing yield. Application of NPK with or without systemic insecticides at 15- 20 days interval reduced disease incidence and increased the yield by 82 to 90%.
Biological and molecular characterization of a Tospovirus isolate from tomato and its relationship with other Tospoviruses
K. UMAMAHESWARAN, R.K. JAIN*, A.I. BHAT and Y.S. AHLAWAT
Indian Phytopathology , 2012,
Abstract: A Tospovirus was isolated from tomato (Lycopersicon esculentum Mill) plants showing severe necrosis of leaves and growing buds. Host range studies indicated that the tomato Tospovirus isolate was closely related to Groundnut bud necrosis virus (GBNV). Sequence analyses of nucleocapsid protein (N) gene showed that the amino acid sequence identity with GBNV was 96% while the identities with other Tospoviruses were in the range of 16-85%. On this basis, it is concluded that the tomato Tospovirus should be considered as a strain of GBNV and designated as GBNV-To.
Comparison of isolates of Indian citrus ring spot virus and Citrus psorosis virus
SHELLY PRAVEEN, GARY A. BARTHE*, K.S. DERRICK and Y.S. AHLAWAT
Indian Phytopathology , 2012,
Abstract:
Diagnosis of Tomato leaf curl virus by polymerase chain reaction
SHELLY PRAVEEN, M.S. KULKARNI SOBUDH K. SINHA and Y.S. AHLAWAT
Indian Phytopathology , 2012,
Abstract:
Association of Carla- and Poty-viruses with mosaic disease of elephant foot yam
Y.S. AHLAWAT, R.P. PANT* and K.N. BHAGAWATI
Indian Phytopathology , 2012,
Abstract: The genus Amorphophallus (family Araceae) consists of 90 species but widely cultivated species in the tropics is A. companulatus. It is cultivated throughout the Indian subcontinent for vegetable purposes. The major cultivation of A. companulatus isdone in the North-eastern and southern states. Dasheen mosaic virus (DsMV) a member of potyvirus group infecting yam has been reported from several countries'. During survey in Assam, virus disease like symptoms were observed in A. companulatus. The variety Kovur showed 80 percent incidence. The symptoms include mottling, rolling and crinkling of leaves. The leaves show cup-like structure due to inward and upward rolling of leaf margins. Brownish chlorotic spots of 0.1 to 0.2 cm in diameter appear on the leaf lamina. The diseased leaves become leathery, brittle and severely reduced. Infected plants become stunted. The size and weight of corm reduced to 70 percent.
Association of a Cariavirus with mosaic disease of football lily in India
D.R. DAS, R.P. PANT* and Y.S. AHLAWAT
Indian Phytopathology , 2012,
Abstract:
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