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Search Results: 1 - 10 of 526911 matches for " D. M.;Destro "
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Listeria monocytogenes: UM AGENTE INFECCIOSO AINDA POUCO CONHECIDO NO BRASIL
C. D. CRUZ,M. B. MARTINEZ,M. T. DESTRO
Alimentos e Nutri??o , 2009,
Abstract: Listeria monocytogenes o agente infeccioso respons vel pela doen §a de origem alimentar denominada listeriose. Apesar de baixa incid ancia, a listeriose representa importante risco sa ode p oblica, pelo grau de severidade das seq elas e alto -ndice de mortalidade (20% a 30%) que promove em popula § μes de risco, como pacientes imunocomprometidos, idosos e gestantes. No Brasil e em outros pa -ses em desenvolvimento, al m da falta de preocupa § £o por parte das autoridades de sa ode p oblica em rela § £o sua dissemina § £o, n £o h estat -sticas oficiais de casos de listeriose, pois sua notifi ca § £o n £o obrigat 3ria. Considerando o aumento da incid ancia de L. monocytogenes no mundo todo, e a falta de informa § μes atualizadas na l -ngua portuguesa sobre o comportamento desta bact ria, sua preval ancia, fatores de virul ancia e outros aspectos relevantes para a sa ode p oblica, se elaborou esta revis £o.
Biochemical characteristics of typical and atypical Staphylococcus aureus in mastitic milk and environmental samples of Brazilian dairy farms
Silva, Wladimir Padilha da;Destro, Maria Teresa;Landgraf, Mariza;Franco, Bernadette D.G.M.;
Brazilian Journal of Microbiology , 2000, DOI: 10.1590/S1517-83822000000200008
Abstract: this study reports results on the biochemical characteristics of 274 staphylococcus aureus strains isolated from mastitic milk, hands of milkmen and milking machines. features included colony morphology on baird parker agar (bpa), catalase, coagulase and thermonuclease tests. api-staph (biomérieux, france) was used as reference identification system. 39.4% of the colonies on bpa identified as s.aureus were atypical. they were more frequent in milk samples (94.4%). all s.aureus strains were coagulase positive but intensity of the test varied according to the source: environmental strains were 3+ or 4+, while 17.1% of strains isolated from milk presented only 1+ or 2+ results. the majority of atypical s.aureus strains presented a 4+ coagulase result. 2.2% of s.aureus strains were thermonuclease negative. sixteen thermonuclease positive and coagulase positive strains were identified as s.hyicus.
Biochemical characteristics of typical and atypical Staphylococcus aureus in mastitic milk and environmental samples of Brazilian dairy farms
Silva Wladimir Padilha da,Destro Maria Teresa,Landgraf Mariza,Franco Bernadette D.G.M.
Brazilian Journal of Microbiology , 2000,
Abstract: This study reports results on the biochemical characteristics of 274 Staphylococcus aureus strains isolated from mastitic milk, hands of milkmen and milking machines. Features included colony morphology on Baird Parker agar (BPA), catalase, coagulase and thermonuclease tests. API-Staph (bioMérieux, France) was used as reference identification system. 39.4% of the colonies on BPA identified as S.aureus were atypical. They were more frequent in milk samples (94.4%). All S.aureus strains were coagulase positive but intensity of the test varied according to the source: environmental strains were 3+ or 4+, while 17.1% of strains isolated from milk presented only 1+ or 2+ results. The majority of atypical S.aureus strains presented a 4+ coagulase result. 2.2% of S.aureus strains were thermonuclease negative. Sixteen thermonuclease positive and coagulase positive strains were identified as S.hyicus.
Determina o da dose de radia o gama para reduzir a popula o de Salmonella spp em carne de frango
Santos A.F.,Vizeu D.M.,Destro M.T.,Franco B.D.G.M.
Ciência e Tecnologia de Alimentos , 2003,
Abstract: O consumo de carne de frango contaminada com Salmonella é uma causa importante de salmonelose em todo o mundo. Essa doen a transmitida por alimentos, é um problema de saúde pública e causa de perdas econ micas substanciais. O processo de irradia o é um método eficiente de conserva o de alimentos por reduzir o número de microrganismos patogênicos e deteriorantes, sem que as características organolépticas e nutricionais do alimento sejam alteradas significativamente. Os objetivos desta pesquisa foram determinar o valor D10 de Salmonella Typhimurium ATCC 14028, inoculada em sobrecoxas de frango, e recomendar uma dose de radia o para ser aplicada a esse alimento a fim de torná-lo seguro do ponto de vista microbiológico. O valor D10 foi calculado a partir da curva de sobreviventes dessa bactéria em sobrecoxas de frango, após terem sido expostas às doses de 0kGy; 0,1kGy; 0,2kGy; 0,3kGy; 0,5kGy; 0,6kGy; 0,7kGy e 0,8kGy. O valor D10 variou de 0,241kGy a 0,480kGy. Considerando o maior valor D10 e o maior nível de contamina o de Salmonella spp encontrado em sobrecoxas de frango - 0,4NMP/g - adquiridas em feiras livres da cidade de S o Paulo, a dose de radia o gama recomendada para garantir a seguran a do produto em rela o à presen a de Salmonella spp é de 3,8kGy.
Inactivation of Escherichia coli O157:H7 in hamburgers by gamma irradiation
Chirinos, Rodolfo R.O.;Vizeu, Dirceu M.;Destro, Maria Teresa;Franco, Bernadette D.G.M.;Landgraf, Mariza;
Brazilian Journal of Microbiology , 2002, DOI: 10.1590/S1517-83822002000100011
Abstract: escherichia coli o157:h7 causes bloody diarrhoea, haemorrhagic colitis and life-threatening complications like haemolytic uremic syndrome and thrombotic thrombocitopenic purpura. among foods associated with outbreaks caused by this pathogen, hamburger is the most common one. the aim of this research was to determine the radiation dose to reduce the population of e. coli o157:h7 in hamburgers to non-detectable levels in order to render a safer product. hamburgers, inoculated with escherichia coli o157:h7, were exposed to gamma radiation (60co) treatment, with doses ranging from 0 to 0.7 kgy. the average temperature during the process was 5.6oc. non-inoculated hamburgers were submitted to sensory evaluation after being exposed to irradiation doses of 0.8 kgy and 1.0 kgy. the d10 for the pathogen varied from 0.17 kgy to o.27 kgy in hamburger. considering the highest d10 value in hamburger, a dose of 1.08 kgy would be sufficient to reduce e. coli o157:h7 contamination in 4 log cycles, without affecting the sensory attributes of the product.
Inactivation of Escherichia coli O157:H7 in hamburgers by gamma irradiation
Chirinos Rodolfo R.O.,Vizeu Dirceu M.,Destro Maria Teresa,Franco Bernadette D.G.M.
Brazilian Journal of Microbiology , 2002,
Abstract: Escherichia coli O157:H7 causes bloody diarrhoea, haemorrhagic colitis and life-threatening complications like haemolytic uremic syndrome and thrombotic thrombocitopenic purpura. Among foods associated with outbreaks caused by this pathogen, hamburger is the most common one. The aim of this research was to determine the radiation dose to reduce the population of E. coli O157:H7 in hamburgers to non-detectable levels in order to render a safer product. Hamburgers, inoculated with Escherichia coli O157:H7, were exposed to gamma radiation (60Co) treatment, with doses ranging from 0 to 0.7 kGy. The average temperature during the process was 5.6oC. Non-inoculated hamburgers were submitted to sensory evaluation after being exposed to irradiation doses of 0.8 kGy and 1.0 kGy. The D10 for the pathogen varied from 0.17 kGy to O.27 kGy in hamburger. Considering the highest D10 value in hamburger, a dose of 1.08 kGy would be sufficient to reduce E. coli O157:H7 contamination in 4 log cycles, without affecting the sensory attributes of the product.
A comparison of ready-to-use systems for evaluating the microbiological quality of acidic fruit juices using non-pasteurized orange juice as an experimental model
Ramazotti Ferrati,Analí; Tavolaro,Paula; Destro,Maria Teresa; Landgraf,Mariza; Franco,Bernadette D.G.M.;
International Microbiology , 2005,
Abstract: several alternative analytical methods are currently available for the rapid microbiological testing of food. due to their many advantages, particularly their convenience of use, the popularity of ready-to-use systems for the enumeration of hygiene indicator microorganisms is increasing. however, the ability of these systems to enumerate stressed microorganisms, such as those that may be found growing in acidic foods, is unknown. therefore, the aim of this study was to evaluate the performance of petrifilm? and simplate? plates for the enumeration of total aerobes and fungi (yeasts and molds) in acidic fruit juices, using non-pasteurized orange juice as an experimental model. the samples were analyzed before and after neutralization of ph, and the results were compared with those obtained using conventional procedures, i.e. pour-plates containing standard methods agar, acidified potato dextrose agar, or dichloran-glycerol agar. the results obtained with petrifilm and simplate for counts of mesophilic aerobes as well as for yeast and mold correlated well with those obtained using conventional procedures. although no statistically significant differences were observed between counts of non-neutralized and neutralized samples (α ≤0.05), better correlation indexes were observed in the neutralized samples. both petrifilm and simplate proved to be good alternative methods for testing the microbiological quality of acidic fruit juices.
"Quantifica??o de Listeria monocytogenes em salames fatiados embalados a vácuo"
Ichiro Sakate,Ricardo; Casale Aragon,Lina; Raghiante,Fernanda; Landgra,Mariza; Bernadette D. G. M,Franco; Destro,Maria Teresa;
Archivos Latinoamericanos de Nutrición , 2003,
Abstract: summary. occurrence of listeria monocytogenes in pre-sliced vacuum-packaged salami in s?o paulo - brazil. there is scarce information in brazil and other south american countries about the occurrence of listeria monocytogenes in food, mainly refrigerated ready-to-eat products. the consumption of sliced vacuum-packaged meat products has increased in the last few years. nevertheless, a complete assessment of the risk associated with l. monocytogenes in these products is still necessary. because of the production and storage characteristics of these products, they can be considered potential vehicles for l. monocytogenes to humans, mainly immunocompromised, elderly, and pregnant women. the objectives of this study was to evaluate the population of l. monocytogenes in salami, a ready-to-eat meat product with extended shelf life, acquired in retail stores in s?o paulo - brazil. the three-tube most probable number technique was used and the methodology was that from health canada. strains were biochemically identified and serotyped. among the 45 samples, 3 (6,7%) harboured 9,2 mpn/g of l. monocytogenes and the others < 0,3 mpn/g. all the strains belonged to serotypes 1/2a and 1/2b, the most frequent serotypes found in food everywhere. even being low, the population of l. monocytogenes found in this product could be a cause of concern to public health authorities as it can pose a threat to population at risk. this contamination highlights the importance of implementing systems like haccp to assure safe products to consumers.
Characterization of class 1 integrons and antibiotic resistance genes in multidrug-resistant Salmonella enterica isolates from foodstuff and related sources
Ribeiro, Vinicius B.;Lincopan, Nilton;Landgraf, Mariza;Franco, Bernadete D.G.M.;Destro, Maria T.;
Brazilian Journal of Microbiology , 2011, DOI: 10.1590/S1517-83822011000200033
Abstract: in recent years, an increase in the occurrence of antimicrobial resistance among salmonella enterica has been observed in several countries, which is worrisome because s. enterica is one of the most common causes of human gastroenteritis worldwide. the aim of this study was to characterize class 1 integrons and antibiotic resistance genotypes in salmonella enterica isolates recovered from foodstuff and related sources. nineteen multidrug-resistant (mdr) salmonella enterica isolates were recovered. higher resistance rates to tetracycline (90%), streptomycin (80%), sulfamethoxazole-trimethoprim (80%), ampicillin (60%) and nalidixic acid (70%) were related to the presence of the teta, aada, sul1/sul2, blatem-1 genes, and a codon mutation at position 83 of the gyra gene, respectively. class 1 integrons harboring aada, blatem-1, sul1 or dhfr1 genes were detected in nine (45%) salmonella enterica strains belonging to serotypes brandenburg, panama, agona, mbandaka and alachua. finally, clonal dissemination of s. panama, s. derby and s. mbandaka was confirmed by pfge. detection of clonally related mdr salmonella enterica suggests that endemic serotypes can be supported by class 1 integron-borne gene cassettes and/or mutations in drug targets. emergence and dissemination of multidrug-resistant salmonella enterica can have a major public health impact in an environment where large-scale suppliers ship their products.
Epidemiological Survey of Listeria monocytogenes in a gravlax salmon processing line
Cruz, C.D.;Silvestre, F.A.;Kinoshita, E.M.;Landgraf, M.;Franco, B.D.G.M.;Destro, M.T.;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000200032
Abstract: listeria monocytogenes is a cause of concern to food industries, mainly for those producing ready-to-eat (rte) products. this microorganism can survive processing steps such as curing and cold smoking and is capable of growing under refrigeration temperatures. its presence in rte fish products with extended shelf life may be a risk to the susceptible population. one example of such a product is gravlax salmon; a refrigerated fish product not exposed to listericidal processes and was the subject of this study. in order to evaluate the incidence and dissemination of l. monocytogenes 415 samples were collected at different steps of a gravlax salmon processing line in s?o paulo state, brazil. l. monocytogenes was confirmed in salmon samples (41%), food contact surfaces (32%), non-food contact surfaces (43%) and of food handlers' samples (34%), but could not be detected in any ingredient. 179 l. monocytogenes isolates randomly selected were serogrouped and typed by pfge. most of l. monocytogenes strains belonged to serogroup 1 (73%). 61 combined pulsotypes were found and a dendrogram identified six clusters: most of the strains (120) belonged to cluster a. it was suggested that strains arriving into the plant via raw material could establish themselves in the processing environment contaminating the final product. the wide dissemination of l. monocytogenes in this plant indicates that a great effort has to be taken to eliminate the microorganism from these premises, even though it was not observed multiplication of the microorganism in the final product stored at 4oc up to 90 days.
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