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Search Results: 1 - 10 of 804293 matches for " D. M. A.;Bicudo "
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Hematological changes in sheep inoculated with natural and Cobalt60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)
Netto, D. P.;Chiacchio, S. B.;Bicudo, P. L.;Alfieri, A. A.;Balarim, M. R. S.;Nascimento, N.;
Journal of Venomous Animals and Toxins including Tropical Diseases , 2004, DOI: 10.1590/S1678-91992004000100004
Abstract: natural (nv) and cobalto60-irradiated (irv) crotalus durissus terrificus venom were used to evaluate serum production capacity of sheep and possible hematological and biochemical effects. freeze-dried venom aliquots were diluted in acidified saline solution (nacl 150 mm, ph 3.0) and irradiated by a cobalt 60 source at a dose of 5.54 x 102 gy/h and a concentration of 2.000 gy. twelve sheep were divided into two groups of six animals. one group received irradiated venom (irv) and the other natural venom (nv). three antigen doses (venom) were administered at monthly intervals. blood samples were collected weekly for analysis of serum neutralization potency and capacity, complete blood count (cbc), total plasma protein, fibrinogen, albumin, and globulin. at the end of the experiment, the animals were challenged with a ld50 for sheep and showed no signs of envenoming. the two groups did not present clinical alterations. results of the total leukocyte count did not present interaction or time factor effect for both groups, but there was a different action between them, with the nv group presenting more cells than the irv group. the leukocyte increase to 13,000/ml indicates that slight leukocytosis occurred in the week after the first inoculation in the nv group. there was no statistically significant difference between groups in the absolute count of segmented neutrophils, eosinophils, and lymphocytes but there were statistically significant oscillations in values at the different collecting times. the nv group presented an increase in the absolute neutrophil count after the first inoculation that persisted for 5 weeks. in the irv group, the increase in neutrophils occurred only in the first week returning to normal in the following weeks. the alterations in the neutrophil count are indicative of systemic inflammatory response related to cytokine release; response was more marked in the nv group, showing its greater toxicity.
Síntese e estudo microestrutural de perovsquitas tipo La0,8Ca0,2MO3 (M: Co e Mn) para aplica??o em catálise automotiva
Ribeiro, J. P.;Borges, F. M. M.;Pimentel, P. M.;Oliveira, R. M. P. B.;Melo, D. M. A.;Bicudo, T. C.;
Ceramica , 2011, DOI: 10.1590/S0366-69132011000300014
Abstract: materials with perovskite structure are potential catalysts for preventing greenhouse gas emissions to the environment. several methods have been proposed for the synthesis of these materials in order to produce homogeneous powders with nanometric particle size. in the present work, the la0.8ca0.2mno3 and la0.8ca0.2coo3 systems were prepared by the polymeric precursor method for application in automotive catalysis. this method was chosen because it allows obtaining homogeneous powders and crystalline phases at lower temperatures. the powders were calcined at 700 and 900 oc for 4 h and characterized by thermal analysis, x-ray diffraction and scanning electron microscopy techniques. the perovskites are nanometric, single phased and present suitable properties for use in automotive catalysis.
Medical Students Progress in the Practice Assessment of Knowledge, Skills, and Attitudes  [PDF]
Smrr Passeri, L. M. Li, W. Nadruz Jr., A. M. Bicudo
Creative Education (CE) , 2015, DOI: 10.4236/ce.2015.68084
Abstract: We have developed an alternate instrument of evaluation named ACHA-Avalia??o de Conhecimento, Habilidades e Atitude (Assessment of Knowledge, Skills and Attitude) in our institution. We feel that the construct of this tool is more comprehensive than OSCE (Objective Structured Clinical Examination), as it includes two other domains; knowledge and attitude. This study aims to present results of ACHA in medical students during internship as a tool of monitoring their progress. We selected students enrolled in Medicine course from 2007 to 2011. We included in the analysis only 5th and 6th year students, and only those who participated in four consecutive assessments in this period of time. There was a linear progression during the four assessments. The lowest averages were found in the first tests, during the fifth and sixth year (I5Y and I6Y). The scores were separated by stations (Surgery, Internal Medicine, Obstetrics/Gynecology, and Pediatrics) to evaluate student progress. There was a significant difference between assessments in all specialties (the highest value in the II5Y assessment for Surgery (Mean = 7.43 SD = 1.59), and the lowest in the I5Y for Pediatrics (Mean = 4.49, SD = 2.33). The best correlation of improvement progression (score over time) was observed in the Internal Medicine (R2 = 0,678), while the poorest was seen in Gynecology/Obstetrics (R2 = 0.144). We felt that ACHA went beyond being only an assessment tool for evaluating student performance, but also it involved other domains of education and learning process. Perhaps the key element would be the acceptance by everyone involved (teachers and students) in the process which forced a reflection and developed actions to improve the quality of the course and the evaluation itself. We understand that it is essential for the evaluation process to be dynamic and for such the motivation of those involved is vital.
An unusual gangrenous goat mastitis caused by Staphylococcus aureus, Clostridium perfringens and Escherichia coli co-infection
Ribeiro, M.G.;Lara, G.H.B.;Bicudo, S.D.;Souza, A.V.G.;Salerno, T.;Siqueira, A.K.;Geraldo, J.S.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2007, DOI: 10.1590/S0102-09352007000300037
Abstract: relata-se, pela primeira vez no brasil, a ocorrência de mastite gangrenosa caprina atípica causada pela co-infec??o por staphylococcus aureus, clostridium perfringens e escherichia coli em uma cabra da ra?a boer, na segunda semana de lacta??o. descrevem-se os achados clínicos, os procedimentos de diagnóstico microbiológico e a conduta terapêutica.
Effect of sublethal doses of the insecticide imidacloprid on adaptive traits of Drosophila melanogaster: Response to treatment over and after 15 consecutive generations  [PDF]
Thais de Fran?a Patarro, Antonio José Manzato, Lílian Madi-Ravazzi, Hermione E. M. de Campos Bicudo
Open Journal of Animal Sciences (OJAS) , 2013, DOI: 10.4236/ojas.2013.34A2002
Abstract: A sublethal dose of Imidacloprid, considered actually as the most widely used insecticide against biting and sucking insects, was administered to Drosophila melanogaster for detecting effects on biological traits. The choice of this species as organism-model potentially opens the possibility to explore more deeply the processes involved in those effects because, among other reasons, there is a large accumulation of biological knowledge on this species and because it propitiates multiple approaches in laboratory and nature. The flies were treated along 15 consecutive generations. F1 parents were randomly taken among virgin flies from the stocks, but the parents of the successive generations were the first 15 couples emerged in the previous one. The number of progeny (productivity) and the duration of the emergence period were analyzed in every generation revealing insecticide toxicity in 12 of the 15 generations. The observation of an increase in the number of progeny over the generations, which occurred in both control and treated experiments (although maintaining higher productivity in the control), suggested an effect of the use of the first 15 emerged couples in successive generations. A comparative analysis of the mortality of the F15 adult flies exposed to imidacloprid by contact, which involved flies from the control, treatment and from the stocks that originated the experiments, reinforced this idea, indicating a genetic interplay of the emergence speed with productivity and adult tolerance to the insecticide, a subject that may be better explored in another study. Toxicity was also observed for the traits longevity, viability during development from egg to adult and oviposition rate. Considering the present intensive use of imidacloprid, the harmful effects observed in these important biological characteristics may be considered able to decrease the adaptive value of D. melanogaster populations exposing them at risk of decline.
Medroxyprogesterone acetate or long-acting progesterone in the biostimulation of lambs
Claudia D. Monteiro,Sony D. Bicudo,Hugo S. Toma,Carmo E.A. Biscarde
Italian Journal of Animal Science , 2010, DOI: 10.4081/ijas.2010.e64
Abstract: The aim of this study was to evaluate the response of prepubertal ewe lambs to exogenous administration of either medroxyprogesterone acetate (MAP) or long-acting progesterone (LAP) together with biostimulation. Two Pool Dorset adult males and 75 mixed-breed prepubertal ewe lambs (average of 179 days-old and 30.0kg) were used. The females were randomly assigned to three different groups. In the first group the females were submitted to the insertion of intravaginal sponges containing MAP (60 mg) for 12 days and were then biostimulated for eight weeks. In the second group the females were submitted to a single injection of LAP (225 mg) and then to biostimulation for eight weeks. In the last group, the females were only submitted to biostimulation for eight weeks. Animals were considered cyclic when plasma progesterone (P4) concentration exceeded 1.0 ng/mL in at least one of two consecutive blood samples taken within a 7-day interval in three distinct experimental moments. After treatments 93.3% of the females disregarding their group started their cyclicity and most of them (92.0%), continued to be cyclic after 63 days of either MAP or LAP together with biostimulation under both male and female effect. We conclude that prepubertal ewe lambs when submitted to protocols of either MAP or LAP followed by biostimulation result in puberty at the 7 month of age. It can be deducted that some ewe lambs submitted to the administration of either MAP or LAP together with biostimulation promoted a multiplier effect upon the other young females that were then stimulated to start cyclicity.
Humoral response and neutralization capacity of sheep serum inoculated with natural and Cobalt 60-irradiated Crotalus durissus terrificus venom (Laurenti, 1768)
Netto, D. P.;Chiacchio, S. B.;Bicudo, P. L.;Alfieri, A. A.;Nascimento, N.;
Journal of Venomous Animals and Toxins , 2002, DOI: 10.1590/S0104-79302002000200009
Abstract: the aim of this work was to investigate antigen irradiation on crotalic antivenom and the capacity of sheep as serum producers. twelve sheep in two groups of six were inoculated with crotalus durissus terrificus venom. one group was inoculated with natural venom (nv) and the other with cobalt 60 gamma-irradiated venom (irv). three antigen doses were given to the animals at monthly intervals for immunization. the toxic activity of the venom was assessed by ld50 determination in mice. blood samples were collected weekly analyses of serum neutralization capacity and potency. at the end of the experiment, the animals were challenged with a ld50 for sheep showed no signs of envenoming. these results showed that toxicity of the irradiated venom was 4.4 times less than the natural venom. the sera from the irradiated group neutralized ld50 14.6 times, and the sera from the natural group 4.4 times. sera from the irradiated group were five times more potent. the two groups did not present clinical alterations. the results of this study show the potential for using sheep in crotalic antivenom production. the use of irradiated venom in sheep immunization induces a powerful and lasting humoral immune response shown by both the in vitro neutralization and potency tests and by the indirect elisa antibody level detection technique.
Concentra??es plasmáticas de testosterona, triiodotironina (T3) e tiroxina (T4) em bodes submetidos ao estresse calórico
Coelho, L.A.;Sasa, A.;Bicudo, S.D.;Balieiro, J.C.C.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2008, DOI: 10.1590/S0102-09352008000600007
Abstract: to verify the effect of heat stress (hs) on plasma testosterone, triiodothyronine (t3), and thyroxine (t4) concentrations, eight saanen (n=4) and alpine brown (n=4) bucks were kept in climate chamber under thermal neutral conditions (13.0oc to 26.7oc) for 30 days. after a resting period (60 days), the same bucks were submitted to heat stress (23.7oc to 34.0oc) for another 30 days. to neutralize the seasonal variations of hormonal profiles throughout the period, the photoperiod was controlled every 30 days altering long (16 hours of light and 8 hours of darkness) and short days (8 hours of light and 16 hours of darkness). the blood samples were collected twice a week during five weeks. in both breeds, there was no effect of hs (p>0.05) on plasma concentrations of testosterone (1.8±0.2 vs 1.3±0.2ng/ml) and t4 (52.7±2.8 vs 50.0±2.8ng/ml). there was a decline (p<0.01) of plasma t3 concentrations (1.3±0.1 vs 1.0±0.1ng/ml) after hs treatment, but this reduction was only evident in saanen bucks. in both breeds, the plasma concentrations of t3 and t4 varied (p<0.01) according to the day of blood sample collection. the hs was sufficient to provoke a physiological response with reduction of plasma concentrations of thyroid hormones mainly of t3 in saanen bucks, but not in alpine ones. the hs did not affect the plasma testosterone and t4 levels.
Preamble "Water and chemical fluxes through catchments"
M. C. Forti, D. C. Bicudo, C. Bourotte, V. de Cicco,F. C. S. Arcova
Hydrology and Earth System Sciences (HESS) & Discussions (HESSD) , 2005,
Abstract: Two areas in the Atlantic Forest (S o Paulo State, Brazil), with contrasting environments in respect of human occupation, were monitored from 1999 to 2001. One area named PEFI (23°38'08''-23°40'18'' S and 46°36'48''-46°38'00'' W) at an altitude of 798 m a.s.l., 526.4 ha in area and about 50 km from the sea, lies in a State Park within the largest metropolis of South America - S o Paulo. The other area, named CUNHA (between 23°13'18'' and 23°16'10'' S and 45°02'53'' and 45°05'15'' W) about 1050 m a.s.l. with an area of 2854 ha and about 15 km from the sea, is also within a State Park in the Atlantic Forest, but is surrounded by rural areas and small villages. For each area, the rainfall and throughfall chemistry were examined and pH and Na+, K+, Mg2+, Ca2+, NH4+, Cl-, NO3- and SO42- as well as trace metals were determined. Compared with PEFI, CUNHA is characterised by low chemical fluxes and the largest differences are for the ions such as Ca2+, H+, NO3- and SO42- which are mainly anthropogenic in origin. Differences in throughfall chemical fluxes are linked to the nutritional status of the trees.
Gradiente litoraneo-limnético do fitoplancton e ficoperifíton em uma lagoa da planície de inunda??o do Rio Mogi-Gua?u
Taniguchi, Glória M;Bicudo, Denise C;Senna, Pedro A.C;
Brazilian Journal of Botany , 2005, DOI: 10.1590/S0100-84042005000100011
Abstract: study aimed at evaluating the phytoplankton and phycoperiphyton structure and dynamics during a hydrological cycle at the littoral-limnetic gradient in the diogo pond, and oxbow lake of the mogi-gua?u river floodplain (s?o paulo state, brazil). samplings were carried out during one hydrological cycle (high water, flooding, low water, and drought) in three sites (open water, littoral-limnetic interface, and within the macrophyte stand) for phytoplankton, and in the littoral region for phycoperiphyton associated to eicchornia azurea kunth. algal classes densities and biomass (chlorophyll-a) were analyzed for both communities. principal component analysis separated the two major hydrological periods: the rainy and the dry seasons. rainy season was associated to higher temperatures, higher water levels, lower nitrate, and higher densities of euglenophyceae, cryptophyceae, chrysophyceae, and chlorophyll-a concentrations. disturbance regime during high water and flood periods favoured opportunistic groups represented by phytoflagellates. moreover, phytoplankton from littoral region was separated during high water, low water and drought phases indicating the influence of the spatial gradient. periphyton showed a distinct trend, with higher biomass and densities during low water and drought phases. hydrological cycle was considered the main driving force on the phytoplankton and periphyton structure as well as on the interaction and interchanges of these communities in the oxbow pond.
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