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Search Results: 1 - 10 of 199793 matches for " Cynthia Priscilla do Nascimento Bonato e Cláudia Regina Sharan "
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EVALUATION OF ANEUPLOIDOGENIC POTENTIAL OF ANTINEOPLASIC TAXOL DEMONSTRATED ON DIPLOID STRAINS OF Aspergillus nidulans.
Simone Jurema Ruggeri Chiuchetta, Cynthia Priscilla do Nascimento Bonato e Cláudia Regina Sharan
SaBios : Revista de Saúde e Biologia , 2006,
Abstract: Taxol is a drug isolated from the rind of Taxus brevifolia plant. In eukaryotic cells this drug, used as a chimiotherapic agent, hyper stabilizes the microtubules by its association with beta-tubulin protein. Cell becomes incapable to use cytoskeleton in a flexible way. Thus, it blocks the cellular cycle and modifies the normal segregation of chromosomes during mitoses. In this research, a diploid strain of Aspergillus nidulans fungus was used to evaluate the aneuploidogenic potential of taxol, as well as the toxicities of this drug. The results have demonstrated that taxol, in concentrations of 0,06mM and 0,18mM, is capable to induce aneuploidy.
Estudo histopatológico do trajeto de biópsia de tumores musculoesqueléticos malignos
Ribeiro, Marcelo Barbosa;Oliveira, Cláudia Regina G.C.M. de;Filippi, Renée Zon;Baptista, André Mathias;Caiero, Marcelo Tadeu;Saito, Carlos Fernando;Nascimento, Sérgio Antonio Barbosa do;Camargo, Olavo Pires de;
Acta Ortopédica Brasileira , 2009, DOI: 10.1590/S1413-78522009000500005
Abstract: objective: to investigate whether there is any risk of seeding by neoplasic cells along biopsy tracks and to study local histological abnormalities. methods: this was a prospective study on biopsy tracks in patients who underwent operations due to malignant musculoskeletal tumors between april 2006 and april 2007. results: neoplasic cell implantation occurred in 32% of the tracks. the most common histological abnormalities in positive cases were classified as severe fibrosis, mild inflammatory component and severe neovascularization. conclusion: we suggest that traditional oncological resection of the track should be carried out together with excision of the specimen.
O acolhimento à família na Unidade de Terapia Intensiva: conhecimento de uma equipe multiprofissional
Josiane de Jesus Martins,Eliane Regina Pereira do Nascimento,Cláudia Koch Geremias,Dulcineia Ghizoni Schneider
Revista Eletr?nica de Enfermagem , 2008,
Abstract: Estudo exploratório descritivo, de abordagem qualitativa, que objetivou investigar o conhecimento dos profissionais de saúde de uma Unidade de Terapia Intensiva (UTI) sobre o acolhimento à família. Foi realizado entre os meses de setembro a novembro de 2007 em uma UTI localizada na regi o Sul do país, com a participa o de profissionais de enfermagem, médicos, assistente social e auxiliar administrativo. Os dados foram coletados através de entrevista e, para a análise, adotou-se o referencial metodológico do Discurso do Sujeito Coletivo (DSC), sendo identificados como temas centrais: significado da família; cuidado à família; dificuldades para cuidar da família; significado de acolhimento. Evidenciou-se que a percep o dos participantes a respeito da família vai além de la os de consangüinidade, que o cuidado à família se dá através de informa es e orienta es; que as dificuldades para o cuidado à família est o relacionadas ao medo do envolvimento e ao despreparo; que o acolhimento é entendido como proximidade, cuidar o outro como a si próprio, e ser receptivo. Os resultados apontam para a valoriza o do acolhimento à família como uma tecnologia de cuidado relevante para a prática em UTI, porém existe a necessidade de capacita o dos profissionais.
The frequency of nasal injury in newborns due to the use of continuous positive airway pressure with prongs
Nascimento, Renata Medeiros do;Ferreira, Anne Laura Costa;Coutinho, Ana Cláudia Ferreira Pinheiro;Veríssimo, Regina Célia Sales Santos;
Revista Latino-Americana de Enfermagem , 2009, DOI: 10.1590/S0104-11692009000400009
Abstract: continuous positive airway pressure (cpap) with prongs is the ventilatory support most used in newborns. nasal injuries are complications that may arise due to the prolonged use of this device. this study aimed to determine the frequency of nasal injuries in newborns through the use of continuous positive airway pressure with prongs. a convenience sample composed of hospitalized newborns using prongs for more than two days was used. data were collected through a structured form. lesions were observed in all newborns, which were classified as: mild (79.6%), moderate (19.7%) and severe (0.7%). the conclusion is that the use of prongs for more than two days represents a risk factor for the lesions to develop.
High-Fat Fish Oil Diet Prevents Hypothalamic Inflammatory Profile in Rats
Gustavo Duarte Pimentel,Fábio Santos Lira,José César Rosa,Cláudia Maria Oller do Nascimento,Lila Missae Oyama,Regina Lúcia Harumi Watanabe,Eliane Beraldi Ribeiro
ISRN Inflammation , 2013, DOI: 10.1155/2013/419823
Abstract: Whether PUFA diets affect inflammatory mediators in central and peripheral sites is not clear. We investigated the effect of high-fat PUFA diets on the expression of proteins involved in inflammatory pathways in hypothalamus, muscle, and liver. Male rats were fed for 2 months with either chow or high-fat diets enriched with either soy (n-6 PUFAs) or fish oil (n-3 PUFAs). The fish group had normal body weight, low serum NEFA, reduced hypothalamic levels of TNF-α, IL-6, and TRAF6, and increased levels of IL-10 receptor. In contrast, the soy group had increased body weight and hypothalamic levels of TRAF6 and NFκBp65. In muscle, the fish diet reduced TNF-α and IL-6 levels. Both PUFA diets increased muscle IL-10 levels and reduced liver TNF-α and IL-6 levels. The data showed that the high-fat soy diet induced activation of the hypothalamic NFκB inflammatory pathway, a feature predisposing to feeding and energy expenditure disturbances associated with the development of obesity. On the other hand, the high-fat fish diet improved the central and the peripheral inflammatory profile via reduction of intracellular inflammatory mediators, suggesting a protection against obesity. 1. Introduction Obesity is known to present an inflammatory process of low grade, with elevated levels of cytokines such as interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), and interleukin 1 beta (IL-1β), contributing to the pathogenesis of important disturbances of the obese condition, as insulin resistance and metabolic defects. This inflammatory state has been described to induce elevated signaling through the toll-like receptors TLR2 and TLR4, with activation of the nuclear factor κB (NFκB) pathway in muscle, liver, and adipose tissue [1–4]. In this inflammatory pathway, TLR2/4 binding to the myeloid differentiation factor-88 (MyD88) leads, after some intermediate steps, to the recruitment of the tumor necrosis factor receptor-associated factor-6 (TRAF6). Its interactions with several proteins leads to phosphorylation of the inhibitory factor IκB, which is then targeted for proteosomal degradation, releasing NFκB, whose p65 subunit undergoes phosphorylation and translocates to the nucleus, where it binds to its target genes to produce proinflammatory cytokines [5]. Importantly, prolonged intake of saturated or trans fats has also been associated with NFκB/MyD88 pathway-mediated induction of inflammatory cytokines in the hypothalamus and cytokine-induced impairment of central insulin hypophagia [6–10]. The hypothalamus is a key regulator of energy homeostasis, through the
Estudo da prevalência de úlceras perianais em pacientes com AIDS e fatores associados
Nascimento Maria Cláudia do
Revista da Sociedade Brasileira de Medicina Tropical , 1999,
Abstract:
Effects of a ferment soy product on the adipocyte area reduction and dyslipidemia control in hypercholesterolemic adult male rats
Nadia Cheik, Elizeu Rossi, Ricardo Guerra, Neuli Tenório, Cláudia Oller do Nascimento, Fabiana Viana, Marla Manzoni, Iracilda Carlos, Patrícia Le?o da Silva, Regina Vendramini, Ana Damaso
Lipids in Health and Disease , 2008, DOI: 10.1186/1476-511x-7-50
Abstract: Over a period of 8 weeks, animals had "ad libitum" food intake and water consumption as well as body weight and food consumption was monitored. The animals were assigned to four different experimental groups: Control Group (C); Control + Fermented Product Group (CPF); Hypercholesterolemic diet group (H); and Hypercholesterolemic + Fermented Product Group (HPF). The HPF and CPF groups received an intragastric administration of 1 ml of fermented product daily. After the experimental period the animals were killed by decapitation, blood was collected to measure cholesterol, triglycerides and HDL-cholesterol plasma concentration. Adipocyte circumference, lipolysis and lipogenis rates were measures using epididymal and retroperitoneal white adipose tissues.The results demonstrated that 1 ml/day/rat of the fermented soy product promoted important benefits such as reduced cholesterolemia in hypercholesterolemic diet group and the adipocyte circumference in both control and hypercholesterolemic diet group.The fermented soy product enriched with Enterococcus faecium and Lactobacillus Jugurti decreased circulating lipids levels and reduced adipocyte area in rats.The term probiotic refers to live micro-organisms which when administered in adequate amounts confer a health benefit on the host [1]. Probiotic bacteria have been the focus of much scientific and commercial interest due to a range of possible health effects of these bacteria such as on lipid metabolism [2,3]. The most widely studied probiotic bacteria were Lactobacillus GG, Lactobacillus acidophilus, Bifidobacterium bifidum and Enterococcus faecium [2-4].Probiotic dairy products are considered to have functional properties because the probiotic bacteria added to the regular fermentation cultures provide therapeutic benefits such as modification of the immune system, reduction in cholesterol, alleviation from lactose intolerance and faster relief from diarrhea [5].Available data on the effects of probiotic bacteria on
Classical osteoblastoma, atypical osteoblastoma, and osteosarcoma: a comparative study based on clinical, histological, and biological parameters
Oliveira, Cláudia Regina Gomes Cardim Mendes de;Mendon?a, Berenice Bilharino;Camargo, Olavo Pires de;Pinto, Emilia Modolo;Nascimento, Sérgio Antonio Barbosa;Latorre, Maria do Rosario D. O.;Zerbini, Maria Claúdia Nogueira;
Clinics , 2007, DOI: 10.1590/S1807-59322007000200012
Abstract: objective: to investigate the biological behavior of classical and atypical osteoblastomas in comparison to osteosarcomas. methods: based on histological parameters, 30 osteoblastomas were subclassified as classical osteoblastomas (23/30) or atypical osteoblastoma (high cellularity, prominent blue osteoid, and epithelioid osteoblasts-7/30). comparative immunohistochemical and clinical analysis was performed in 17 cases of patients with high-grade osteosarcoma. formalin-fixed, paraffin-embedded archival tissue was immunostained for p53 and proliferation cell nuclear antigen. tumors with positive p53 stain underwent molecular analyses for fragments of exon 10. results: the mean proliferating cell nuclear antigen indexes for classical osteoblastoma, atypical osteoblastoma, and osteosarcoma were 33%, 61%, and 79%, respectively. the atypical subgroup showed similar results to those of the osteosarcoma group (p < 0.001). p53 protein was detected in 4 (13%) osteoblastomas (3 of these were atypical osteoblastoma), and 4 osteosarcomas (23%) also showed p53 positivity. dna mutation performed in p53-positive cases was confirmed in exon 10 in 2 atypical osteoblastomas (2/3), 1 classical osteoblastoma (1/1), and 1 osteosarcoma (1/4). disease recurrence was correlated with p53 expression (p = 0.009), atypical subtype (p = 0.031), spiculated blue bone on histology (p = 0.018), and proliferatingcell nuclear antigen labeling index > 40 (p = 0.015). conclusion: these results validate atypical osteoblastoma as an entity, with p53 and proliferation cell nuclear antigen immunoexpression closer to that of osteosarcoma than of classical osteoblastoma. proliferating cell nuclear antigen labeling index and p53 may be useful predictors of recurrence.
Acute exhaustive exercise regulates IL-2, IL-4 and MyoD in skeletal muscle but not adipose tissue in rats
José C Rosa Neto, Fábio S Lira, Nelo E Zanchi, Lila M Oyama, Gustavo D Pimentel, Ronaldo VT Santos, Marília Seelaender, Cláudia M Oller do Nascimento
Lipids in Health and Disease , 2011, DOI: 10.1186/1476-511x-10-97
Abstract: Rats were killed by decapitation immediately (E0 group, n = 6), 2 (E2 group, n = 6) or 6 (E6 group, n = 6) hours after the exhaustion protocol, which consisted of running on a treadmill at approximately 70% of VO2max for fifty minutes and then at an elevated rate that increased at one m/min every minute, until exhaustion.The control group (C group, n = 6) was not subjected to exercise. IL-2 protein expression increased at E0 in the soleus and EDL; at E2, this cytokine returned to control levels in both tissues. In the soleus, IL-2 protein expression was lower than that in the control at E6. IL-4 protein levels increased in EDL at E6, but the opposite result was observed in the soleus. MyoD expression increased at E6 in EDL.Exhaustive exercise was unable to modify IL-2 and IL-4 levels in MEAT and RPAT. The results show that exhaustive exercise has different effects depending on which muscle is analysed.Exercise promotes physiological changes in response to disturbances in homeostasis [1]. These changes do not cease until a small overcompensation is attained, showing that exercise provides an excellent model for the study of physiological stress and the adaptive capacity of the body [2]. Acute and training exercises modulate cytokine levels in the serum [3] and other tissues, such as adipose tissue and skeletal muscle [4-6].IL-2 and IL-4 are cytokines involved in immune response. IL-2, a pro-inflammatory cytokine, has multiple functions in the inflammatory response, including activation of immune cell effectors and stimulation of the number of white blood cells on the endothelial surface of skeletal muscle [7]. In addition, it is a potent inducer of the proliferation, differentiation, development, survival, memory and regulatory functions of T-lymphocytes [8].In contrast, IL-4 is a pleiotropic cytokine that is important in the anti-inflammatory response. T-cells, mast cells and neutrophils are the main producers of IL-4 [9,10]. It has been reported that other cells, s
β-Hydroxy-β-methylbutyrate (HMβ) supplementation stimulates skeletal muscle hypertrophy in rats via the mTOR pathway
Gustavo D Pimentel, José C Rosa, Fábio S Lira, Nelo E Zanchi, Eduardo R Ropelle, Lila M Oyama, Cláudia M Oller do Nascimento, Marco de Mello, Sergio Tufik, Ronaldo VT Santos
Nutrition & Metabolism , 2011, DOI: 10.1186/1743-7075-8-11
Abstract: The amino acid leucine has been shown to stimulate skeletal muscle protein synthesis and attenuate muscle proteolysis. Some of these benefits have been attributed to the conversion of leucine to its metabolite β-hydroxy-β-methylbutyrate (HMβ) [1-4], which has been shown to be anti-catabolic and effective at attenuating muscle atrophy during exercise stress [2,5,6] and in models of cancer [4,7], congestive heart failure, sepsis, and HIV [8,9]. According to a review published by Nelo et al. [10], the dose of HMβ supplementation used in the majority of the previous studies to achieve these effects was 3 g/day of HMβ; use of this dose is based on evidence that it produces better results than 1.5 g/day.HMβ is produced by the transamination of α-ketoisocaproate (KIC), which is metabolised to isovaleryl-CoA by the enzyme KIC dioxygenase. The cytosolic dioxygenase enzyme differs from the mitochondrial KIC dehydrogenase enzyme in several ways. KIC dioxygenase produces free HMβ in the cytosol, whereas the dehydrogenase enzyme produces the CoA derivative of isovaleric acid in the mitochondria [11,12]. Quantitatively, it is estimated that approximately 5% of all L-leucine oxidised in the human body is converted to HMβ. Therefore, a subject weighing 70 kg produces approximately 0.2 - 0.4 g HMβ/day, depending on the content of leucine in the diet. Because leucine, like all essential amino acids, is not synthesised in the human body, HMβ is produced from dietary protein [13].The results of trials evaluating the effect of HMβ supplementation on reversing cachexia associated with rheumatoid arthritis [14], HIV [8], and muscle damage [15,16] are performed merely with biochemical markers. Additionally, most of the available studies concerning the effects of HMβ are related to effects on skeletal muscle, with an absence of relevant information concerning consequences in other peripheral tissues (e.g., adipose tissue and liver).Recently, Holecek et al. [17] demonstrated increases in pro
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