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Search Results: 1 - 10 of 2419 matches for " Cristiane LGF;Goveia "
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First record of molluscs naturally infected with Angiostrongylus cantonensis (Chen, 1935) (Nematoda: Metastrongylidae) in Brazil
Caldeira, Roberta Lima;Mendon?a, Cristiane LGF;Goveia, Christiane Oliveira;Lenzi, Henrique L;Graeff-Teixeira, Carlos;Lima, Walter S;Mota, Ester M;Pecora, Iracy Lea;Medeiros, Aline Maria Zigiotto de;Carvalho, Omar dos Santos;
Memórias do Instituto Oswaldo Cruz , 2007, DOI: 10.1590/S0074-02762007000700018
Abstract: seeking the identification of angiostrongylus cantonensis as a potential etiological agent of three clinical cases of eosinophilic meningitis, mollusc specimens were collected in the state of espírito santo, brazil. the snails were identified as sarasinula marginata (45 specimens), subulina octona (157), achatina fulica (45) and bradybaena similaris (23). larvae obtained were submitted to polymerase chain reaction and restriction fragment length polymorphism diagnosis. their genetic profile were corresponded to a. cantonensis. rattus norvegicus experimentally infected with third-stage larvae, developed menigoencephalitis, and parasites became sexually mature in the lungs. additionally, larvae obtained from a. fulica snails, from s?o vicente, state of s?o paulo, also showed genetic profiles of this nematode. this is the first record of brazilian molluscs infected with this nematode species.
Penetration Sites and Migratory Routes of Angiostrongylus costaricensis in the Experimental Intermediate Host (Sarasinula marginata)
Mendon?a Cristiane LGF,Carvalho Omar S,Mota Ester M,Pelajo-Machado Marcelo
Memórias do Instituto Oswaldo Cruz , 1999,
Abstract: The intermediate hosts of Angiostrongylus costaricensis are terrestrian molluscs, mostly of the family Veronicellidae. The present work aimed at clarifying more accurately the sites of penetration and the migratory routes of A. costaricensis in the tissue slugs and at verifying the pattern of the perilarval reaction at different times of infection. Slugs were individually infected with 5,000 L1, and killed from 30 min to 30 days after infection. From 30 min up to 2 hr after infection, L1 were found within the lumen of different segments of the digestive tube having their number diminished in more advanced times after exposition until complete disappearance. After 30 min of exposition, percutaneous infection occurred, simultaneously to oral infection. Perilarval reaction was observed from 2 hr of infection around larvae in fibromuscular layer, appearing later (after 6 hr) around larvae located in the viscera. A pre-granulomatous reaction was characterized by gradative concentration of amebocytes around larvae, evolving two well-organized granulomas. In this work we confirmed the simultaneous occurrence of oral and percutaneous infections. Perilarval reaction, when very well developed, defined typical granulomatous structure, including epithelioid cell transformation. The infection also caused a systemic mobilization of amebocytes and provoked amebocyte-endothelium interactions.
Penetration Sites and Migratory Routes of Angiostrongylus costaricensis in the Experimental Intermediate Host (Sarasinula marginata)
Mendon?a, Cristiane LGF;Carvalho, Omar S;Mota, Ester M;Pelajo-Machado, Marcelo;Caputo, Luzia FG;Lenzi, Henrique L;
Memórias do Instituto Oswaldo Cruz , 1999, DOI: 10.1590/S0074-02761999000400022
Abstract: the intermediate hosts of angiostrongylus costaricensis are terrestrian molluscs, mostly of the family veronicellidae. the present work aimed at clarifying more accurately the sites of penetration and the migratory routes of a. costaricensis in the tissue slugs and at verifying the pattern of the perilarval reaction at different times of infection. slugs were individually infected with 5,000 l1, and killed from 30 min to 30 days after infection. from 30 min up to 2 hr after infection, l1 were found within the lumen of different segments of the digestive tube having their number diminished in more advanced times after exposition until complete disappearance. after 30 min of exposition, percutaneous infection occurred, simultaneously to oral infection. perilarval reaction was observed from 2 hr of infection around larvae in fibromuscular layer, appearing later (after 6 hr) around larvae located in the viscera. a pre-granulomatous reaction was characterized by gradative concentration of amebocytes around larvae, evolving two well-organized granulomas. in this work we confirmed the simultaneous occurrence of oral and percutaneous infections. perilarval reaction, when very well developed, defined typical granulomatous structure, including epithelioid cell transformation. the infection also caused a systemic mobilization of amebocytes and provoked amebocyte-endothelium interactions.
Ammonia emissions in tunnel-ventilated broiler houses
Lima, KAO;Moura, DJ;Carvalho, TMR;Bueno, LGF;Vercellino, RA;
Revista Brasileira de Ciência Avícola , 2011, DOI: 10.1590/S1516-635X2011000400008
Abstract: gas production in broiler houses and their emissions are closely related to the microclimate established inside the house according to air temperature, humidity, and velocity. therefore, the internal house environment is influenced by building typology and ventilation system. the objective of the present study was to evaluate ammonia emission rates in broiler houses equipped with different ventilation systems (negative or positive pressure) and litter conditions (new or built-up). the environment of six commercial broiler houses was evaluated internal and external nh3 concentrations. ventilation rates were recorded to estimate ammonia emission rates. the efficiency of circulation and exhaust fans was assessed, and higher ventilation rates were determined in negative-pressure houses due to the higher flow of the fans. houses with new litter increased ammonia emission rates along the rearing period, indicating the relationship between gas emissions, bird age and ventilation rates, and presented a typical curve of nh3 emission increase. negative-pressure houses with built-up litter presented higher emission rates during the first rearing week due to the high nh3 concentration during the brooding period, when the ventilation rates required to maintain chick thermal comfort are low. although the results of the present study indicate an advantage of the positive-pressure systems as to gas emissions, further research is needed reduce gas emissions in broiler houses with negative-pressure systems.
Microbiological evaluation of different antiseptic povidone-iodine and chlorhexidine formulations after intentional contamination of containers
Padovani, Christiane Moreira;Graziano, Kazuko Uchikawa;Goveia, Vania Regina;
Revista Latino-Americana de Enfermagem , 2008, DOI: 10.1590/S0104-11692008000600016
Abstract: this study aimed to evaluate the survival rate of microorganisms within different antiseptic formulations - povidone-iodine (pvp-i) and chlorhexidine (chx) - after intentional contamination, and to establish the minimum care necessary to ensure sterilization of non-disposable antiseptic solution containers. a laboratory study was performed with 180 antiseptic containers, which were contaminated with serratia marcescens [1 x 105 ufc/ml]. the containers were closed and stored, at room temperature, during seven days and shaken daily. the antiseptic cultures were evaluated to be 100% negative to serratia marcescens in all of the non-disposable containers. these results suggested that antiseptic solutions inactivate microorganisms [1 x 105 ufc/ml]. since cleaned antiseptic containers have around 102 ufc coming from tap water, it can be inferred that cleansing is a safe minimum procedure to ensure reuse of containers for distribution of chx and pvp-i solutions in aqueous, detergent and alcoholic formulations.
Low-temperature sterilization and new technologies
Goveia, Vania Regina;Pinheiro, Silma Maria Cunha;Graziano, Kazuko Uchikawa;
Revista Latino-Americana de Enfermagem , 2007, DOI: 10.1590/S0104-11692007000300002
Abstract: the new low-temperature sterilization technologies are presented as an alternative to the use of ethylene oxide. this review was performed in order to identify evidences of the antimicrobial activity, toxicity, adverse events and the applicability of these technologies. the research was carried through the electronic databases medline and lilacs up to 2005. the authors analyzed 10 articles in this survey. the studies about the efficacy of these sterilization methods constitute experimental and comparative research that showed the influence of the extension and diameter of the lumen, besides the presence of crystal salts. thus, choosing the correct equipment is essential, as well as the assurance of the cleansing of the devices, which interfere with the effectiveness of the low-temperature sterilization. these technologies present limitations regarding the sterilization of graft bone and affect the materials properties.
Litter materials and the incidence of carcass lesions in broilers chickens
Garcia, RG;Almeida Paz, ICL;Caldara, FR;N??s, IA;Bueno, LGF;Freitas, LW;Graciano, JD;Sim, S;
Revista Brasileira de Ciência Avícola , 2012, DOI: 10.1590/S1516-635X2012000100005
Abstract: the aim of this study was to evaluate the effects of different litter materials on litter compaction, broiler feathering and the incidence of carcass lesions. in the experiment, 3240 one-day-old ross? chicks were selected by sex and distributed according to a completely randomized experimental design in a 2 x 6 factorial arrangement (two sex and six litter materials). the following litter materials were used: wood shavings, rice husks, chopped napier grass, 50% sugar cane bagasse plus 50% wood shavings, 50% sugar cane bagasse plus 50% rice husks, and pure sugar cane bagasse. litter compaction was weekly assessed using a penetrometer. on days 21, 35 and 42 of the experimental period, feathering on the back and legs was scored according to a 0 - 10 scale. on day 42, birds were slaughtered and the presence of bruises, scratches and footpad lesions was recorded. litter material had no effect on bird feathering. carcass lesions (scratches, bruises and footpad lesions) were influenced by the litter material evaluated. birds reared on sugarcane bagasse and chopped napier grass presented more scratches, bruises and footpad lesions than the others. dermatitis was more evident in birds reared on sugarcane bagasse, chopped napier grass and the combination of litter materials. it was found that males presented higher incidence of dermatitis and footpad lesions than females. each litter material presented different compaction degrees, which increased along the experimental period. sugarcane bagasse, chopped napier grass and the combination of bedding materials presented the highest degree of compaction, compared with wood shavings and rice husks.
Uso de furadeiras elétricas domésticas em cirurgias ortopédicas
Goveia, Vania Regina;Ribeiro, Silma Maria Cunha Pinheiro;Graziano, Kazuko Uchikawa;
Acta Ortopédica Brasileira , 2007, DOI: 10.1590/S1413-78522007000300009
Abstract: domestic ordinary drilling machines have been used in orthopedic surgeries in brazilian hospitals, for bone drilling. however, it is an electric thermo-sensitive appliance, non-specific for surgical use, not assessed in terms of sterilization efficiency, as well as on potential surgical infections associated to its use. the objective of this review was to describe the state-of-the-art use of domestic drills in orthopaedic surgeries, considering the difficulties of cleaning and sterilization. the lilacs and medline databases were consulted using mesh and free medical terms, without time restraints. two specific articles were found about the use of domestic electric drills in orthopaedic surgeries. in the first article, the author suggests the disposal of the equipment in cases of any noticeable blood contamination that could result in difficulties to clean it. the second article describes how to adapt a domestic drilling machine for orthopaedic surgery, but the sterilization process is performed by using formalin tablets at room conditions. further studies are required for evaluating the current practice and to recommend safe conditions for its use or towards giving it up.
A ventila??o de furadeiras elétricas constitui fonte de contamina??o para a cirurgia?
Goveia, Vania Regina;Pinto, Flávia Morais Gomes;Graziano, Kazuko Uchikawa;
Acta Ortopédica Brasileira , 2009, DOI: 10.1590/S1413-78522009000300006
Abstract: objective: to evaluate the potential risk of surgical contamination by the venting port of ordinary electric drills (ed) employed in orthopaedic surgeries. materials and methods: an experimental laboratory, randomized study was developed to analyze eds in surgical practice and new cleaned and sterilized equipment, which were contaminated with bacillus atrophaeus spores at a concentration of 84 x 106 ufc. the air generated by the engine of each drill was collected and cultivated on sterile agar plates. results: positive culture was identified in two ed in surgical practice, as well as a positive culture to bacillus atrophaeus with 1 cfu growth (1,19 x 10-8). conclusion: in the conditions of the experiment, the airgenerated by the venting port of the ed's engine does not consist of a source of contamination for the surgical site.
Reprocessamento de cateteres cardíacos: uma revis?o
Ribeiro, Silma Maria Cunha Pinheiro;Graziano, Kazuko Uchikawa;Alfa, Michelle M.;Goveia, Vania Regina;
Revista Brasileira de Cirurgia Cardiovascular , 2006, DOI: 10.1590/S0102-76382006000300014
Abstract: hemodynamic catheters are widely reused mainly in developing countries where the cost of new devices is very high. scientific publications point to an absence of validated cleaning and sterilization processes and there is a consensus that reusing these devices causes physical, chemical and funcional damage. so what is the evidence related to the use of this kind of catheter? the objective of this study is to identify the scientific evidence related to the effects of reprocessing. a search for publications in english, portuguese and spanish was performed in medline/pubmed and lilacs using medical subject headings (mesh) terms and free terms without stipulating restraints on time. in total 21 papers were analized. it was found that there is commonly damage to the surface polymers as identified by eletronic microscopy. failure in the cleaning and sterilization process was identified by the presence of debris and microrganisms at the end of the procedure. the results of this study are very important when choosing to reuse hemodynamic catheters.
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