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Search Results: 1 - 10 of 383417 matches for " Cristiana Nicoli de;Rêgo "
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Replacement of fetal calf serum by human serum as supplementation for human fibroblast culture
Isaac, César;Mattos, Cristiana Nicoli de;Rêgo, Francinni Mambrine Pires do;Cardim, Larissa Nocchi;Altran, Silvana Cereijido;Paggiaro, André Oliveira;Tutihashi, Rafael Mamoru Carneiro;Mathor, M?nica Beatriz;Ferreira, Marcus Castro;
Revista Brasileira de Cirurgia Plástica , 2011, DOI: 10.1590/S1983-51752011000300003
Abstract: introduction: fetal calf serum (fcs) is commonly used as a supplement in the culture medium for fibroblast cells. this supplementation is far from ideal as sample quality varies from batch to batch and the composition of fcs is not completely known. in addition, fcs may be contaminated with viruses and/or prions and may also cause adverse immunologic responses in humans. due to these facts, a worldwide effort is being made to find alternatives for xenobiotic elements in cell cultures. human serum could be a safer alternative, especially for clinical application. methods: we investigated human serum as a substitute for fcs in human fibroblast culture. fresh human serum was obtained from 10 healthy volunteers. fibroblasts were cultivated in multiwell plates containing either dulbecco's modified eagle's medium (dmem) plus 10% fcs (d10) or dmem plus 10% human serum (d10h). cell counts were obtained between 24 and 264 hours of cultivation; results were expressed as the mean number of cells a± standard error of the mean to create cell proliferation curves. results: there was no statistical difference in fibroblast proliferation between the two groups. human serum supported human fibroblast growth and proliferation, suggesting that it may be a potential substitute for fcs in human cell culture. cells cultivated with human serum presented a different morphology, appearing smaller and more rounded as compared to cells cultivated in d10. conclusions: these results demonstrate that human serum can be substituted for fcs in human fibroblasts culture and that fibroblasts cultivated in the presence of human serum have a morphology that is similar to in vivo fibroblasts.
Avalia o, pela ressonancia magnética, da resposta quimioterápica neoadjuvante no cancer de mama localmente avan ado
Rêgo Salete de Jesus Fonseca
Radiologia Brasileira , 2001,
Abstract:
Exame citológico do líquido céfalo-raquidiano no diagnóstico de neoplasias primitivas ou metastáticas do sistema nervoso: a propósito de seis casos
Moraes-Rêgo, Sydney F. de;Moraes-Rêgo, Kátia G. de;
Arquivos de Neuro-Psiquiatria , 1986, DOI: 10.1590/S0004-282X1986000200008
Abstract: the authors report 6 cases of cerebrospinal fluid (csf) examinations in which malignant cells were found. in 5 cases the finding was incidental: medulloblastoma (case 1); malignant melanoma (case 2); adenocarcinoma (case 3); meningitis carcinomatosa (case 4) and neuroleukaemia (case 5). in only one case neuroleukaemia was suspected before the study of the csf (case 6). the unexpected occurrence of malignant cells in the csf demands the pathologist to. be well acquainted with tumor cell cytology, in order to identify them providing so useful information that can decidedly influence subsequent diagnostic and therapeutic procedures. the cell collection technic developed in the authors' laboratory was considered adequate, because both inflammatory and malignant cells were securely identified. its principle is to obtain thin air-dried smears that dry almost instantaneously on slides, in order the celular structure be preserved. after centrifuging about 5-8ml of csf the tube is inverted so as to pour off the whole of the supernatant fluid. it is kept inverted with the operator's left hand, so that no drop of fluid can run back on to the cells. with his right hand the operator touches the bottom of the inverted tube with a pasteur micropipette preciously made by distending a microhematocrit tube under the flame of a bunsen burner and attached with adhesive tape to the handle of a wire loop 2mmin diameter. one little drop of the cell suspension is blown with an hematologic suction tube on a slide and spread out with the loop in the maner of a comb, taking care not to scratch over the same place twice, as it dries almost instantaneously. only a square centimeter or so need be covered. the smear is stained with giemsa or leishman stain.
Nesting Behavior of Centris (Hemisiella) vittata Lepeletier (Hymenoptera: Apidae) in an area of the cerrado in the northeast of the State of Maranh?o, Brazil
Ramos, Marina;Albuquerque, Patrícia de;Rêgo, Márcia;
Neotropical Entomology , 2010, DOI: 10.1590/S1519-566X2010000300011
Abstract: the nesting behavior of centris (hemisiella) vittata lepeletier was studied in the urbano santos cerrado in the northeast of maranh?o state, brazil. to date, this species has only been studied in trap-nests. the nesting behavior of this species in a natural condition is recorded for the first time. nesting occurred in preexisting holes in dead trunks of aroeira, astronium myracrodruon (anacardiaceae), at the end of the rainy season. the cells were constructed with a mixture of sand and oil. after finishing the nests, females used only oil to line the entrance wall. two females collected sand at the same time to build their nests, and another one was seen collecting resources at byrsonima sp. (malpighiaceae). the pollen load of one bee was analyzed and was found to contain mainly hymenaea courbaril (leg-caesalpiniaceae). development from egg to adult took about 60 days. protandry was observed, being males smaller than females. the nests were parasitized by coleoptera and diptera. however, the females only showed aggressive behavior against females of the same species or genus.
Community of male Euglossini bees (Hymenoptera: Apidae) in a secondary forest, Alcantara, MA, Brazil
BRITO, C. M. S. de;RêGO, M. M. C.;
Brazilian Journal of Biology , 2001, DOI: 10.1590/S1519-69842001000400012
Abstract: from september, 92 to august, 93 bee sampling was done in a secondary forest near the pepital river, in alcantara, ma, in order to study the local euglossini fauna. five aromatic compounds were used: eucaliptol, eugenol, methyl salicylate, vanillin, and benzoate. four hundred sixty-seven male euglossini bees were captured, distributed in 4 genus and 19 species. euglossa was the most abundant and with high diversity (302 specimens and 14 species), followed by eulaema (121; 3), eufriesea (41; 1), and exaerete (3; 1). the species which more frequently visited the bait were euglossa piliventris (141 specimens; 30.19%), euglossa cingulata (113; 24.21%), euglossa ignita (45; 9.64%), eufriesea pulchra (41; 8.78%), and euglossa gaianii (33; 7.07%) corresponding to 79.88% of the sampling universe. the bees were active throught the year, however during the rainy season more activity and diversity were observed. the most attractive essence was eucaliptol (44.32% specimens and 84.21% species). in spite of this study having been done in a forest fragment, a secondary vegetation area smaller than other areas studied in maranh?o, it showed a significant diversity rate. this result reinforces the importance of fragments in the conservation of local bee communities.
Cor da pele, distin??es e cargos: Portugal e espa?os atlanticos portugueses (séculos XVI a XVIII)
Figueir?a-Rêgo, Jo?o de;Olival, Fernanda;
Tempo , 2011, DOI: 10.1590/S1413-77042011000100006
Abstract: this text shows how the lisbon political core and the colonial elites dealt with the color issue in the atlantic space, especially in respect to the provision of places and even to access to intermediate social distinctions; it investigates how the political core based its views on that theme in several chronologies; it also surveys whether in some locations the presence of native blood among higher social groups modified a simplistic perception of the chromatic variations. since the issue covered grosso modo the strong period (1570-1773) when the blood purity rules were in force in portugal, it discusses the possible connection / contamination between these two realities.
Festa e Turismo Religioso: a prociss o em louvor ao Nosso Senhor dos Passos na cidade de S o Cristóv o (Sergipe - Brasil) (Feast and Religious Tourism) - DOI: 10.5752/P.2175-5841.2011v9n20p96
Ivan Rêgo Arag?o,Janete Ruiz de Macedo
Horizonte : Revista de Estudos de Teologia e Ciências da Religi?o , 2011, DOI: 10.5752/p.2175-5841.2011v9n20p96
Abstract: As festas realizadas pela Igreja Católica foram os primeiros eventos sociais no período do Brasil col nia, agregando pessoas de classes sociais distintas, mesclando as diferentes culturas e etnias. Estas foram motivo de congra amento, mas também de pelejas e controle social realizado pela Igreja e Estado Português. Atualmente no Brasil, as festas católicas deslocam uma grande quantidade de pessoas no período dos seus acontecimentos. Além de atrativos turísticos, as celebra es religiosas no país s o também patrim nio imaterial e est o inseridas dentro da cultura "do festejar" dos brasileiros. Esse presente artigo tem por finalidade abordar aspectos da Prociss o ao Nosso Senhor dos Passos na cidade sergipana de S o Cristóv o. A metodologia utilizada foi a pesquisa bibliográfica, com discuss o no campo teórico sobre Barroco, Festa Devocional, Tradi o, Religiosidade, Prociss o, Turismo Religioso, Cultura e Identidade. Também foi realizada a pesquisa de campo, com observa o direta. Com esse estudo, ficou perceptível que, como território distante de Portugal, as festas religiosas do Brasil tomaram uma dinamica própria, absorvendo elementos profanos e se resignificando através do apelo popular. Palavras-chave: Turismo Religioso; Catolicismo; Festa; Nosso Senhor dos Passos. Abstract The feasties held by the Catholic Church were the first social events during the period of colonial Brazil, bringing people from different social classes, merging the different cultures and ethnicities. These were the subject of harmony, but also of struggles and social control held by the Church and the Portuguese State. Currently in Brazil, the Catholic party moving a lot of people in the period of their events. Apart from tourist attractions, religious celebrations in the country are also intangible heritage and culture are embedded within "the feast" of Brazilians. This present article seeks to address aspects of the Procession of "Nosso Senhor dos Passos" the city of S o Cristóv o state of Sergipe. The methodology used was literature, with discussion in the theoretical field of Baroque, Devotional Feast, Tradition, Religion, Procession, Religious Tourism, Culture and Identity. We also carried out field research, with direct observation.With this study, it was noticeable that, as distant territory of Portugal, the religious feasties in Brazil have taken a very dynamic, absorbing the profane elements and reframing through popular appeal. Key words: Religious Tourism; Catholicism; Feast; Nosso Senhor dos Passos.
S O CRISTóV O E DIVINA PASTORA: LOCUS DO TURISMO RELIGIOSO EM SERGIPE-BRASIL
Ivan Rêgo Arag?o,Janete Ruiz de Macedo
Revista Iberoamericana de Turismo , 2011,
Abstract: O presente trabalho busca analisar a rela o entre religiosidade e turismo nos dois principais núcleos de romaria no estado de Sergipe: S o Cristóv o e Divina Pastora. E perceber a religi o como fazendo parte da cultura. A metodologia utilizada foi pesquisa bibliográfica, embasada no aporte teórico-conceitual sobre Sagrado e Profano, Religiosidade, Turismo Religioso, Prociss o e a pesquisa de campo. Ao estudar o turismo religioso no período destes acontecimentos, percebe-se que, nos dois locais citados, o deslocamento das pessoas é muito mais de ordem simbólica e espiritual, do que pelo prazer de viajar. Nos casos estudados, as quest es referentes à infraestrutura turística n o s o relevantes, visto que, está na tradi o de ambas às festas, a exterioriza o dos sentimentos de sacrifício e de despojamento material.
O PAPEL DA SINERGIA NAS DECIS ES DE FUS ES E AQUISI ES UM ESTUDO DE CASO: DA CRIA O DA AMBEV à INBEV
Ariane Barbosa de Oliveira,Ricardo Bordeaux-Rêgo
Relatórios de Pesquisa em Engenharia de Produ??o , 2011,
Abstract: The objective of this article is to contribute with the theoreticalframework of mergers and acquisitions, by increasing the empirical evidence. A casestudy of the operations that led to the AmBev and InBev was conducted to observe apotential generation of synergy, with the consequent creation of value.The methodology used in this article was the economic and financial indexevaluation and the method of discounted cash flow. It was conclude, by the method ofeconomic and financial index evaluation, which was generated synergy for bothcompanies. But through the analysis of indicators of return to shareholders, it wasfound that they grew only in the case of AmBev. By the method of discounted cash flowit was concluded that there was value creation in the transaction that created AmBev,but the same can not be concluded in the operation which resulted in InBev.
Community of male Euglossini bees (Hymenoptera: Apidae) in a secondary forest, Alcantara, MA, Brazil
BRITO C. M. S. de,RêGO M. M. C.
Brazilian Journal of Biology , 2001,
Abstract: From September, 92 to August, 93 bee sampling was done in a secondary forest near the Pepital River, in Alcantara, MA, in order to study the local Euglossini fauna. Five aromatic compounds were used: eucaliptol, eugenol, methyl salicylate, vanillin, and benzoate. Four hundred sixty-seven male Euglossini bees were captured, distributed in 4 genus and 19 species. Euglossa was the most abundant and with high diversity (302 specimens and 14 species), followed by Eulaema (121; 3), Eufriesea (41; 1), and Exaerete (3; 1). The species which more frequently visited the bait were Euglossa piliventris (141 specimens; 30.19%), Euglossa cingulata (113; 24.21%), Euglossa ignita (45; 9.64%), Eufriesea pulchra (41; 8.78%), and Euglossa gaianii (33; 7.07%) corresponding to 79.88% of the sampling universe. The bees were active throught the year, however during the rainy season more activity and diversity were observed. The most attractive essence was eucaliptol (44.32% specimens and 84.21% species). In spite of this study having been done in a forest fragment, a secondary vegetation area smaller than other areas studied in Maranh o, it showed a significant diversity rate. This result reinforces the importance of fragments in the conservation of local bee communities.
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