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Search Results: 1 - 10 of 224397 matches for " Constantino C. Reyes-Aldasoro "
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The Neutrophil's Eye-View: Inference and Visualisation of the Chemoattractant Field Driving Cell Chemotaxis In Vivo
Visakan Kadirkamanathan, Sean R. Anderson, Stephen A. Billings, Xiliang Zhang, Geoffrey R. Holmes, Constantino C. Reyes-Aldasoro, Philip M. Elks, Stephen A. Renshaw
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0035182
Abstract: As we begin to understand the signals that drive chemotaxis in vivo, it is becoming clear that there is a complex interplay of chemotactic factors, which changes over time as the inflammatory response evolves. New animal models such as transgenic lines of zebrafish, which are near transparent and where the neutrophils express a green fluorescent protein, have the potential to greatly increase our understanding of the chemotactic process under conditions of wounding and infection from video microscopy data. Measurement of the chemoattractants over space (and their evolution over time) is a key objective for understanding the signals driving neutrophil chemotaxis. However, it is not possible to measure and visualise the most important contributors to in vivo chemotaxis, and in fact the understanding of the main contributors at any particular time is incomplete. The key insight that we make in this investigation is that the neutrophils themselves are sensing the underlying field that is driving their action and we can use the observations of neutrophil movement to infer the hidden net chemoattractant field by use of a novel computational framework. We apply the methodology to multiple in vivo neutrophil recruitment data sets to demonstrate this new technique and find that the method provides consistent estimates of the chemoattractant field across the majority of experiments. The framework that we derive represents an important new methodology for cell biologists investigating the signalling processes driving cell chemotaxis, which we label the neutrophils eye-view of the chemoattractant field.
Whole cell tracking through the optimal control of geometric evolution laws
Konstantinos N. Blazakis,Anotida Madzvamuse,Constantino-Carlos Reyes-Aldasoro,Vanessa Styles,Chandrasekhar Venkataraman
Mathematics , 2015,
Abstract: Cell tracking algorithms which automate and systematise the analysis of time lapse image data sets of cells are an indispensable tool in the modelling and understanding of cellular phenomena. In this study we present a theoretical framework and an algorithm for whole cell tracking. Within this work we consider that "tracking" is equivalent to a dynamic reconstruction of the whole cell data (morphologies) from static image datasets. The novelty of our work is that the tracking algorithm is driven by a model for the motion of the cell. This model may be regarded as a simplification of a recently developed physically meaningful model for cell motility. The resulting problem is the optimal control of a geometric evolution law and we discuss the formulation and numerical approximation of the optimal control problem. The overall goal of this work is to design a framework for cell tracking within which the recovered data reflects the physics of the forward model. A number of numerical simulations are presented that illustrate the applicability of our approach.
Drift-Diffusion Analysis of Neutrophil Migration during Inflammation Resolution in a Zebrafish Model
Geoffrey R. Holmes,Giles Dixon,Sean R. Anderson,Constantino Carlos Reyes-Aldasoro,Philip M. Elks,Stephen A. Billings,Moira K. B. Whyte,Visakan Kadirkamanathan,Stephen A. Renshaw
Advances in Hematology , 2012, DOI: 10.1155/2012/792163
Abstract: Neutrophils must be removed from inflammatory sites for inflammation to resolve. Recent work in zebrafish has shown neutrophils can migrate away from inflammatory sites, as well as die in situ. The signals regulating the process of reverse migration are of considerable interest, but remain unknown. We wished to study the behaviour of neutrophils during reverse migration, to see whether they moved away from inflamed sites in a directed fashion in the same way as they are recruited or whether the inherent random component of their migration was enough to account for this behaviour. Using neutrophil-driven photoconvertible Kaede protein in transgenic zebrafish larvae, we were able to specifically label neutrophils at an inflammatory site generated by tailfin transection. The locations of these neutrophils over time were observed and fitted using regression methods with two separate models: pure-diffusion and drift-diffusion equations. While a model hypothesis test (the F-test) suggested that the datapoints could be fitted by the drift-diffusion model, implying a fugetaxis process, dynamic simulation of the models suggested that migration of neutrophils away from a wound is better described by a zero-drift, “diffusion” process. This has implications for understanding the mechanisms of reverse migration and, by extension, neutrophil retention at inflammatory sites. 1. Introduction The fate of neutrophils following completion of the inflammatory programme is of critical importance for the outcome of episodes of acute inflammation and can determine whether there is prompt healing of a wound or the development of chronic inflammation and tissue injury. Neutrophils recruited to sites of inflammation may leave the site or die in situ [1]. The most widely accepted mechanism of neutrophil disposal is the programmed cell death or apoptosis, of the neutrophil followed by macrophage uptake and clearance (reviewed in [2]). Recently, other routes have been proposed; neutrophils may move away from the inflamed site into the bloodstream (“reverse transmigration” [3]), by migration through other tissues (“retrograde chemotaxis” or “reverse migration” [4–6]), or be lost into the inflammatory exudate [7, 8]. Current understanding of the process of reverse migration is reviewed elsewhere [9]. The uncertainty as to the in vivo fates of individual cells relates in part to the difficulty in following individual cells during inflammation resolution in vivo. The transgenic zebrafish model is emerging as a key model for the study of vertebrate immunity [10] and allows direct
PhagoSight: An Open-Source MATLAB? Package for the Analysis of Fluorescent Neutrophil and Macrophage Migration in a Zebrafish Model
Katherine M. Henry, Luke Pase, Carlos Fernando Ramos-Lopez, Graham J. Lieschke, Stephen A. Renshaw, Constantino Carlos Reyes-Aldasoro
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0072636
Abstract: Neutrophil migration in zebrafish larvae is increasingly used as a model to study the response of these leukocytes to different determinants of the cellular inflammatory response. However, it remains challenging to extract comprehensive information describing the behaviour of neutrophils from the multi-dimensional data sets acquired with widefield or confocal microscopes. Here, we describe PhagoSight, an open-source software package for the segmentation, tracking and visualisation of migrating phagocytes in three dimensions. The algorithms in PhagoSight extract a large number of measurements that summarise the behaviour of neutrophils, but that could potentially be applied to any moving fluorescent cells. To derive a useful panel of variables quantifying aspects of neutrophil migratory behaviour, and to demonstrate the utility of PhagoSight, we evaluated changes in the volume of migrating neutrophils. Cell volume increased as neutrophils migrated towards the wound region of injured zebrafish. PhagoSight is openly available as MATLAB? m-files under the GNU General Public License. Synthetic data sets and a comprehensive user manual are available from http://www.phagosight.org.
Estudio cinético de las proteínas en el traumatizado y su correlación con los índices predictivos
Alfonso Alfonso,Lázaro E.; Noda Sardi?as,Constantino L.; Reyes Martínez,Margarita L.; Soroa Espinosa,Yohanka C.;
Revista Cubana de Medicina Militar , 1998,
Abstract: a prospective study was perfomed on 52 traumatized patients, and the correlation between tha values of the predictive index used and the results obtained with the protein fractions studied at admission and on the 3rd and 7th days was established. the following statistical tests were applied: median, standard deviation, minimum, maximum, and the correlation-regresion test, and hypothesis with a confidence interval of 95 % (alpha = 0.05). 38.5 % of the injured patients were within the rank of 21 to 30 years of age and males were found to be predominant (88.5 %). traffic accidents accounted for the most frequent cause of lesions (38.5 %); orthopaedic lesions were found to have the greatest incidence (26.4 %), and complications were found in 38.4 % of the sample. total proteins, albumins, and gamma globulins were found to be significantly reduced 72 hours after the lesions; this reduction was more intense in subjects who died. a correlation between the variables studied and the predictive indices was evidenced.
Estudio cinético de las proteínas en el traumatizado y su correlación con los índices predictivos Kinetic study of proteins in the traumatized patient and its correlation with predictive indeces
Lázaro E. Alfonso Alfonso,Constantino L. Noda Sardi?as,Margarita L. Reyes Martínez,Yohanka C. Soroa Espinosa
Revista Cubana de Medicina Militar , 1998,
Abstract: Se realizó un estudio prospectivo en 52 lesionados, y se estableció la correlación existente entre los valores de los índices predictivos utilizados y los resultados obtenidos en las fracciones protéicas estudiadas al ingreso, 3 y 7 días. Fueron aplicadas las siguientes pruebas estadísticas: medias, desviación estándar, mínimo, máximo y prueba de correlación-regresión e hipótesis con una confiabilidad de 95 % (alfa = 0,05). El 38,5 % de los lesionados correspondieron al rango entre 21 y 30 a os de edad y predominó el sexo masculino (88,5 %). Los accidentes del tránsito constituyeron la causa más frecuente de lesiones (38,5 %); las lesiones ortopédicas fueron las de mayor incidencia (26,4 %) y se encontraron complicaciones en el 38,4 % de la muestra. Las proteínas totales, las albúminas y las globulinas gamma sufrieron disminución significativa a las 72 horas de ocurridas las lesiones, esta depresión se hizo más intensa en los fallecidos y se constató que existe correlación entre las variables estudiadas y los índices predictivos. A prospective study was perfomed on 52 traumatized patients, and the correlation between tha values of the predictive index used and the results obtained with the protein fractions studied at admission and on the 3rd and 7th days was established. The following statistical tests were applied: median, standard deviation, minimum, maximum, and the correlation-regresion test, and hypothesis with a confidence interval of 95 % (alpha = 0.05). 38.5 % of the injured patients were within the rank of 21 to 30 years of age and males were found to be predominant (88.5 %). Traffic accidents accounted for the most frequent cause of lesions (38.5 %); orthopaedic lesions were found to have the greatest incidence (26.4 %), and complications were found in 38.4 % of the sample. Total proteins, albumins, and gamma globulins were found to be significantly reduced 72 hours after the lesions; this reduction was more intense in subjects who died. A correlation between the variables studied and the predictive indices was evidenced.
Atmospheric Pollution in the Tula Industrial Corridor studied using a biomonitor and nuclear analytical techniques
Martínez-Carrillo, M.A.;Solís, C.;Andrade, E.;Beltrán-Hernández, R.I.;Isaac-Olivé, K.;Lucho-Constantino, C.A.;López Reyes, M.C.;Longoria, L.C.;
Revista mexicana de física , 2011,
Abstract: this study deals with the application of nuclear analytical techniques to analyze trace elements in the biological monitor tillandsia usneoides. biological monitors provides an alternative advantageous way of particulate matter sampling in air pollution studies, since there is no need of special sampling devices, accumulation time can be as long as desired. t. usneoides, which occurs naturally throughout méxico, was used to monitor air quality of tula-vito-apasco (tva) industrial corridor at central méxico. this area is considered one of the critical zones of the country because of atmospheric contaminants high concentration. particulate matter is regulated by mexican norms, but its chemical composition is not. plants were transplanted from a clean environment to four sites at the tva corridor, and exposed for 12 weeks from february to april 2008. trace element accumulation of plants was determined by particle induced x ray emission pixe and neutron activa-tion analysis (naa). results reveal differences in trace elements distribution among sites in the tva corridor. furthermore, anthropogenic elements (s, v) and crustal elements (ca) in t. usneoides exhibit high levels. highly toxic elements such as hg, as and cr although present at trace levels, showed un enrichment relative to the initial values, when transplanted to the tva corridor. results show that monitoring with t. usneoides allows a first approximation of air sources to provide insights of the atmospheric pollution in the tva corridor.
El fomento de la ovinocultura familiar en México mediante subsidios en activos: lecciones aprendidas
Martínez-González,Enrique Genaro; Mu?oz-Rodríguez,Manrrubio; García-Mu?iz,José Guadalupe; Santoyo-Cortés,Vinicio Horacio; Altamirano-Cárdenas,J. Reyes; Romero-Márquez,Constantino;
Agronomía Mesoamericana , 2011,
Abstract: the promotion of family sheep-farming in mexico through subsidies in assets: lessons learned. the objective of this work was to determine the impact of subsidies given in assets for the purchase of productive actives in familiar sheep production units (upf) in the state of mexico. a survey was carried-out among 58 sheep farmers in a population of 475 beneficiaries, from january to march of 2007. seventeen months after subsidy release, 16% of upf were closed, 48% were stable or growing, and 36% were contracting. herd dynamics could be explained on the basis of previous experience of the sheep farmer, the capacity of the upf to produce the feed required by the herd, the adoption of new innovative and good practices the production system, the network of knowledge to which the farmer belongs, the sheep quality offered by the network of livestock providers; and the conception of the activity of the producer himself, as savings or as a business. subsidies directed to this activity did not increase productive capacity of the herds, and did not generate wealth or employment.
Caring for Older Persons in a Technologically Advanced Nursing Future  [PDF]
Joseph Andrew Pepito, Rozzano C. Locsin, Rose E. Constantino
Health (Health) , 2019, DOI: 10.4236/health.2019.115039
Abstract: As people live longer, a larger percentage will live with multiple chronic conditions and functional impairments such as difficulties with activities of daily living, mobility, and the management of one’s household. The purpose of this paper is to examine the care of older persons in a technologically advanced nursing future by discussing roles and responsibilities of nurses who practice gerontological nursing, and explaining how a technologically advanced future would change the delivery of home health care for older persons in the community. The theory of Technological Competency as Caring in Nursing grounds 3 processes of nursing as knowing persons as caring, wholeness is oneness, and caring as a multi-dimensional process. Harnessing technology for the health of older persons would enable them to live independently, socially engaged, and safely. A technologically advanced nursing future leads to concomitant sustainable disruptive and frugal innovations in healthcare. Nurses in practice must take advantage of these disruptions and consider frugal innovations as the futures of nursing education, practice, and research are here.
Análisis de la satisfacción laboral de los arquitectos técnicos en el sector de la construcción de la Comunidad Autónoma del País Vasco
CANTONNET,Ma L; IRADI,J; LARREA,A; ALDASORO,J.C;
Revista de la construcción , 2011, DOI: 10.4067/S0718-915X2011000200003
Abstract: the analysis of job satisfaction is a poorly studied variable in the construction industry. this paper aims to fill this gap, and to understand the different variables considered as risk originators of psychosocial origin that influence on job satisfaction of the architects in the construction sector of the basque country (spain). the methodology has been to develop a nonrandom sample of basque architects who were interviewed using a semistructured questionnaire. results are that workers that develop a job that matches with their level of education and work experience have a greater job satisfaction. moreover, the level of satisfaction seems to be directly related to the level of influence over working hours, role clarity, social support at work and a better quality of leadership. it can be concluded. in order to achieve job satisfaction of workers, companies in the construction sector should adapt, from the standpoint of gender, work environments and provide ongoing training to promote the development of people at work.
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