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Search Results: 1 - 10 of 10777 matches for " Claudia Cosio "
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Transcriptome analysis of various flower and silique development stages indicates a set of class III peroxidase genes potentially involved in pod shattering in Arabidopsis thaliana
Claudia Cosio, Christophe Dunand
BMC Genomics , 2010, DOI: 10.1186/1471-2164-11-528
Abstract: The observed increase of total peroxidase activity during development was actually correlated with the induction of only a few class III peroxidase genes which supports the existence of a functional specialization of these proteins. We identified peroxidase genes that are predominantly expressed in one development stage and are probable components of the complex gene networks involved in the reproductive phase. An attempt has been made to gain insight into plausible functions of these genes by collecting and analyzing the expression data of different studies in plants. Peroxidase activity was additionally observed in situ in the silique dehiscence zone known to be involved in pod shattering. Because treatment with a peroxidase inhibitor delayed pod shattering, we subsequently studied mutants of transcription factors (TF) controlling this mechanism. Three peroxidases genes -AtPrx13, AtPrx30 and AtPrx55- were altered by the TFs involved in pod shatter.Our data illustrated the problems caused by linking only an increase in total peroxidase activity to any specific development stage or function. The activity or involvement of specific class III peroxidase genes needs to be assessed. Several genes identified in our study had not been linked to any particular development stage or function until now. Notably AtPrx13, which is one of the peroxidase genes not present on commercially available microarrays. A systematic survey of class III peroxidase genes expression is necessary to reveal specific class III peroxidase gene functions and the regulation and evolution of this key multifunctional enzyme family. The approach used in this study highlights key individual genes that merit further investigation.Genes encoding secreted class III plant peroxidases (EC 1.11.1.7) are present in all land plants and form large multigenic families [1]. In their regular peroxidative cycle, class III peroxidases catalyze the reduction of H2O2 by taking electrons to various donor molecules [2].
Effect of Elodea nuttallii Roots on Bacterial Communities and MMHg Proportion in a Hg Polluted Sediment
Nicole Regier, Beat Frey, Brandon Converse, Eric Roden, Alexander Grosse-Honebrink, Andrea Garcia Bravo, Claudia Cosio
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0045565
Abstract: The objective of this study was to assess the effect of a rooted macrophyte Elodea nuttallii on rhizosphere bacterial communities in Hg contaminated sediments. Specimens of E. nuttallii were exposed to sediments from the Hg contaminated Babeni reservoir (Olt River, Romania) in our microcosm. Plants were allowed to grow for two months until they occupied the entirety of the sediments. Total Hg and MMHg were analysed in sediments where an increased MMHg percentage of the total Hg in pore water of rhizosphere sediments was found. E. nuttallii roots also significantly changed the bacterial community structure in rhizosphere sediments compared to bulk sediments. Deltaproteobacteria dominated the rhizosphere bacterial community where members of Geobacteraceae within the Desulfuromonadales and Desulfobacteraceae were identified. Two bacterial operational taxonomic units (OTUs) which were phylogenetically related to sulfate-reducing bacteria (SRB) became abundant in the rhizosphere. We suggest that these phylotypes could be potentially methylating bacteria and might be responsible for the higher MMHg percentage of the total Hg in rhizosphere sediments. However, SRB were not significantly favoured in rhizosphere sediments as shown by qPCR. Our findings support the hypothesis that rooted macrophytes created a microenvironment favorable for Hg methylation. The presence of E. nuttallii in Hg contaminated sediments should therefore not be overlooked.
Characterization of T Lymphocytes in Chronic Obstructive Pulmonary Disease
Peter J Barnes,Manuel G Cosio
PLOS Medicine , 2004, DOI: 10.1371/journal.pmed.0010020
Abstract:
Canada's implementation of the Paragraph 6 Decision: is it sustainable public policy?
Jillian C Cohen-Kohler, Laura C Esmail, Andre Cosio
Globalization and Health , 2007, DOI: 10.1186/1744-8603-3-12
Abstract: The objective of this research is to investigate whether the CAMR can feasibly achieve its humanitarian objectives given the political interests embedded in the crafting of the legislation. We used a political economy framework to analyze the effect of varied institutions, political processes, and economic interests on public policy outcomes. In-depth, semi-structured interviews were conducted with nineteen key stakeholders from government, civil society and industry. Qualitative data analysis was performed using open-coding for themes, analyzing by stakeholder group.CAMR is removed from the realities of developing countries and the pharmaceutical market. The legislation needs to include commercial incentives to galvanize the generic drug industry to make use of this legislation. CAMR assumes that developing country governments have the requisite knowledge and human resource capacity to make use of the regime, which is not the case. The legislation does not offer sufficient incentives for countries to turn to Canada when needed drugs may be procured cheaply from countries such as India. In the long term, developing and least developing countries seek sustainable solutions to meet the health needs of their population, including developing their own capacity and local industries.CAMR is symbolically meaningful but in practice, limited. The Rwanda case will be noteworthy in terms of the future of the legislation. To meet its intended international health objectives, this legislation needs to be better informed of developing country needs and global pharmaceutical market imperatives. Finally, we contend that serious public policy change cannot strike a balance between all vested interests. Above all, any feasible policy that aims to facilitate compulsory licensing must prioritize public health over trade or economic interests.Following the Implementation of Paragraph 6 of the Doha Declaration on TRIPS and Public Health [1], Canada was among the first countries globally
Effects of 4:1 carbohydrate/protein solution versus a carbohydrate-alone solution on IL-6, TNF-α, and cortisol during prolonged cycling in hot environmental conditions
Cosio-Lima LM, Desai B, Stelzer JW, Schuler PB
Open Access Journal of Sports Medicine , 2012, DOI: http://dx.doi.org/10.2147/OAJSM.S28176
Abstract: ts of 4:1 carbohydrate/protein solution versus a carbohydrate-alone solution on IL-6, TNF-α, and cortisol during prolonged cycling in hot environmental conditions Original Research (8311) Total Article Views Authors: Cosio-Lima LM, Desai B, Stelzer JW, Schuler PB Published Date March 2012 Volume 2012:3 Pages 21 - 26 DOI: http://dx.doi.org/10.2147/OAJSM.S28176 Received: 11 November 2011 Accepted: 18 January 2012 Published: 15 March 2012 Ludmila M Cosio-Lima, Bhargav Desai, John W Stelzer, Petra B Schuler Department of Health, Leisure, and Exercise Science, University of West Florida, Pensacola, FL, USA Purpose: Intense or prolonged exercise and/or heat stress might affect the immune system creating a response similar to trauma or inflammation, resulting in an increase in the susceptibility to viral infections. For example, during prolonged exercise, inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and the stress hormone cortisol are produced and released. Although there have been several studies examining the effects of nutritional supplementation on cytokine release in elite athletes, few studies have investigated the effects of different energy drinks during exercise in adverse environmental conditions. Therefore, the purpose of this study was to compare plasma levels of inflammatory cytokines TNF-α and IL-6, and the stress hormone cortisol, during prolonged cycling under hot environmental conditions while ingesting fluid that contains a ratio of 4:1 carbohydrates and protein (4:1 CHO/PRO) versus a carbohydrate-only drink (CHO). Methods: Six male cyclists (aged 27 ± 8 years; weight 75.5 ± 3.4 kg; VO2max = 66 ± 2.7 mL/kg/min, mean ± standard error) rode on a stationary ergometer on two separate sessions for 2.5 hours at 75% VO2max in an environmental chamber set at 35°C and 60% relative humidity. During the first session the cyclists were given 4 mL/kg body weight of a 6% carbohydrate solution every 15 minutes. During the second session they were given 4 mL/kg body weight of a 4:1 carbohydrate/protein drink every 15 minutes. Subjects were not aware of which drink they were given in each trial. Blood samples were taken pre-, immediately post-, and 12 hours post-exercise. SPSS (IBM Corp, Armonk, NY) was utilized to analyze data through repeated measures analysis of variance. Results: No significant main effect was observed between treatments in either cortisol (P = 0.97), IL-6 (P = 0.64), or TNF-α (P = 0.37) responses. Total cortisol concentrations were significantly elevated (P < 0.05) immediately post-exercise, and from pre- to 12 hours post-exercise with both the 4:1 CHO/PRO and the CHO-alone solutions. TNF-α concentrations were only significantly (P = 0.045) elevated post-exercise with the CHO-alone solution. A significant (P < 0.05) elevation of IL-6 was seen immediately post-exercise and 12 hours post-exercise with both the CHO-alone and 4:1 CHO/PRO solutions. Conclusions: Consuming a 4:1 CHO/PRO solution dur
An investigation of the resolution of inflammation (catabasis) in COPD
Noguera Aina,Gomez Cristina,Faner Rosa,Cosio Borja
Respiratory Research , 2012, DOI: 10.1186/1465-9921-13-101
Abstract: Background Chronic Obstructive Pulmonary Disease (COPD) is characterized by an enhanced inflammatory response to smoking that persists despite quitting. The resolution of inflammation (catabasis) is a complex and highly regulated process where tissue resident macrophages play a key role since they phagocytose apoptotic cells (efferocytosis), preventing their secondary necrosis and the spill-over of their pro-inflammatory cytoplasmic content, and release pro-resolution and tissue repair molecules, such as TGFβ, VEGF and HGF. Because inflammation does not resolve in COPD, we hypothesized that catabasis may be abnormal in these patients. Methods To explore this hypothesis, we studied lung tissue samples obtained at surgery from 21 COPD patients, 22 smokers with normal spirometry and 13 non-smokers controls. In these samples we used: (1) immunohistochemistry to assess the expression of CD44, CD36, VEGF and TGFβ in lung macrophages; (2) real time PCR to determine HGF, PPARγ, TGFβ, VEGF and MMP-9 gene expression; and, (3) ELISA to quantify lipoxin A4, a lipid mediator of catabasis. Results We found that current and former smokers with COPD showed: (1) more inflammation (higher MMP-9 expression); (2) reduced macrophage surface expression of CD44, a key efferocytosis receptor; and, (3) similar levels of TGFβ, VEGF, HGF, PPARγ, and lipoxin A4 than smokers with normal spirometry, despite the presence of inflammation and disease. Conclusions These results identify several potential abnormalities of catabasis in patients with COPD.
Effects of 4:1 carbohydrate/protein solution versus a carbohydrate-alone solution on IL-6, TNF-α, and cortisol during prolonged cycling in hot environmental conditions
Cosio-Lima LM,Desai B,Stelzer JW,Schuler PB
Open Access Journal of Sports Medicine , 2012,
Abstract: Ludmila M Cosio-Lima, Bhargav Desai, John W Stelzer, Petra B SchulerDepartment of Health, Leisure, and Exercise Science, University of West Florida, Pensacola, FL, USAPurpose: Intense or prolonged exercise and/or heat stress might affect the immune system creating a response similar to trauma or inflammation, resulting in an increase in the susceptibility to viral infections. For example, during prolonged exercise, inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, and the stress hormone cortisol are produced and released. Although there have been several studies examining the effects of nutritional supplementation on cytokine release in elite athletes, few studies have investigated the effects of different energy drinks during exercise in adverse environmental conditions. Therefore, the purpose of this study was to compare plasma levels of inflammatory cytokines TNF-α and IL-6, and the stress hormone cortisol, during prolonged cycling under hot environmental conditions while ingesting fluid that contains a ratio of 4:1 carbohydrates and protein (4:1 CHO/PRO) versus a carbohydrate-only drink (CHO).Methods: Six male cyclists (aged 27 ± 8 years; weight 75.5 ± 3.4 kg; VO2max = 66 ± 2.7 mL/kg/min, mean ± standard error) rode on a stationary ergometer on two separate sessions for 2.5 hours at 75% VO2max in an environmental chamber set at 35°C and 60% relative humidity. During the first session the cyclists were given 4 mL/kg body weight of a 6% carbohydrate solution every 15 minutes. During the second session they were given 4 mL/kg body weight of a 4:1 carbohydrate/protein drink every 15 minutes. Subjects were not aware of which drink they were given in each trial. Blood samples were taken pre-, immediately post-, and 12 hours post-exercise. SPSS (IBM Corp, Armonk, NY) was utilized to analyze data through repeated measures analysis of variance.Results: No significant main effect was observed between treatments in either cortisol (P = 0.97), IL-6 (P = 0.64), or TNF-α (P = 0.37) responses. Total cortisol concentrations were significantly elevated (P < 0.05) immediately post-exercise, and from pre- to 12 hours post-exercise with both the 4:1 CHO/PRO and the CHO-alone solutions. TNF-α concentrations were only significantly (P = 0.045) elevated post-exercise with the CHO-alone solution. A significant (P < 0.05) elevation of IL-6 was seen immediately post-exercise and 12 hours post-exercise with both the CHO-alone and 4:1 CHO/PRO solutions.Conclusions: Consuming a 4:1 CHO/PRO solution during prolonged cycling under hot environmenta
Preference of Quinoa Moth: Eurysacca Melanocampta Meyrick (Lepidoptera: Gelechiidae) for Two Varieties of Quinoa (Chenopodium quinoa Willd.) in Olfactometry Assays Preferencia de la Polilla de la Quinua: Eurysacca melanocampta Meyrick (Lepidoptera: Gelechiidae) por dos Variedades de Quinua (Chenopodium quinoa Willd.) en Ensayos de Olfatometría
Juan ?F Costa,Walter Cosio,Maritza Cardenas,Erick Yábar
Chilean Journal of Agricultural Research , 2009,
Abstract: Insects are attracted to plants by visual and olfactory cues. The quinoa moth, Eurysacca melanocampta Meyrick (Lepidoptera: Gelechiidae), is the main insect pest of the quinoa crop, Chenopodium quinoa Willd. (Chenopodiales: Chenopodiaceae), in the southern Peruvian Andes, causing grain yield losses. The aim of this study was to investigate the behavioural response of adult quinoa moths to olfactory stimuli. Specifically, the objectives of this study were: 1) to determine the capacity of E. melanocampta adults of searching for quinoa plants using plant olfactory cues; 2) to determine the preference of E. melanocampta females for the odours derived from two varieties of quinoa: Amarilla de Marangani and Blanca de Junín; and 3) to assess the attraction of male quinoa moths to E. melanocampta females and the host plant in olfactometric bioassays. Adults preferred quinoa plant odour sources in choice tests when distilled water was used as a control (P < 0.0001). Females were more attracted to the Blanca de Junín variety than to Amarilla de Marangani variety (P < 0.05). Males were more attracted to the odour derived from females than to the volatile compounds from plants (both varieties) or to the odour blend derived from plants plus females together. The level of attraction of males towards females is negatively affected by the presence of the quinoa plants. La atracción de insectos hacia las plantas es causada tanto por estímulos visuales como olfativos. La polilla de la quinua, Eurysacca melanocampta Meyrick (Lepidoptera: Gelechiidae), es la principal plaga en el cultivo de quinua, Chenopodium quinoa Willd. (Chenopodiales: Chenopodiaceae), en los Andes del sur peruano causando pérdidas en la producción de granos. El objetivo de este estudio fue evaluar las respuestas conductuales frente a estímulos olfativos de adultos de la polilla de la quinua. Específicamente, los objetivos del estudio fueron: 1) estudiar la capacidad de búsqueda de plantas de quinua de los adultos de E. melanocampta utilizando plantas como estímulos olfativos; 2) determinar la preferencia de hembras de E. melanocampta por olores derivados de plantas de dos variedades de quinua: Amarilla de Marangani y Blanca de Junín; y 3) estudiar la atracción de los machos hacia hembras de E. melanocampta y hacia las plantas hospederas en bioensayos de olfatometría. Los adultos eligieron fuentes de olor de las plantas de quinua en pruebas de elección cuando el control fue agua destilada (P < 0,01). Las hembras fueron más atraídas hacia la var. Blanca de Junín que hacia la var. Amarilla de Marangani (
Framing access to medicines in developing countries: an analysis of media coverage of Canada's Access to Medicines Regime
Laura C Esmail, Kaye Phillips, Victoria Kuek, Andrea Cosio, Jillian Kohler
BMC International Health and Human Rights , 2010, DOI: 10.1186/1472-698x-10-1
Abstract: We conducted a qualitative content analysis of newspaper coverage of the CAMR policy and implementation process from 2003-2008. The primary theoretical framework for this study was framing theory. A total of 90 articles from 11 Canadian newspapers were selected for inclusion in our analysis. A team of four researchers coded the articles for themes relating to access to medicines and which stakeholders' voice figured more prominently on each issue. Stakeholders examined included: the research-based industry, the generic industry, civil society, the Canadian government, and developing country representatives.The most frequently mentioned themes across all documents were the issues of drug affordability, intellectual property, trade agreements and obligations, and development. Issues such as human rights, pharmaceutical innovation, and economic competitiveness got little media representation. Civil society dominated the media contents, followed far behind by the Canadian government, the research-based and generic pharmaceutical industries. Developing country representatives were hardly represented in the media.Media framing obscured the discussion of some of the underlying policy goals in this case and failed to highlight issues which are now significant barriers to the use of the legislation. Using the media to engage the public in more in-depth exploration of the policy issues at stake may contribute to a more informed policy development process. The media can be an effective channel for those stakeholders with a weaker voice in policy deliberations to raise public attention to particular issues; however, the political and institutional context must be taken into account as it may outweigh media framing effects.In September 2003, the Canadian government committed to developing legislation that would facilitate greater access to affordable medicines for developing countries. Over the course of eight months, the legislation, now known as Canada's Access to Medicines Re
Causas y consecuencias de la proteinuria después del trasplante renal
Suárez Fernández,M.L.; G-Cosio,Fernando;
Nefrología (Madrid) , 2011,
Abstract: proteinuria is common following kidney transplantation and affects more than 40% of kidney transplant patients per year. in general, the level of proteinuria is low (<500mg/day) but even those levels significantly reduce graft and patient survival. this is why it is of vital importance to detect proteinuria quickly following transplantation and to investigate its cause. during the same year of the transplant, proteinuria may be caused by multiple factors, including glomerular disease, effects of anti-hla class ii antibodies and drugs such as mtor inhibitors, tubulointerstitial disease of the graft, and significant functional discrepancy between the graft and the recipient. the relationship between proteinuria and graft survival is likely to be due to the factors that cause proteinuria. it is unknown why proteinuria and patient survival are related, but it could be due to a relationship between proteinuria and traditional cardiovascular risk factors or a relationship between proteinuria, endothelial function and inflammation. to treat proteinuria, three aspects should be considered: the cause of proteinuria, the non-specific reduction of proteinuria, and the reduction of the cardiovascular risk.
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