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Search Results: 1 - 10 of 28529 matches for " Cláudia Pombo;Pereira "
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Marcadores RAPD e caracteres morfoagron?micos na determina??o da diversidade genética entre acessos de pimentas e piment?es
Costa, Fabiane Rabelo da;Pereira, Telma Nair Santana;Sudré, Cláudia Pombo;Rodrigues, Rosana;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782008005000099
Abstract: the genetic diversity within collections and banks of germplasm can be estimated by different methods and their choice is dependent of the available resources and the desired precision from the researcher. in the present work, rapd markers and morph-agronomic traits were used to estimate the genetic divergence among 52 capsicum spp. accessions. fifty-seven binary variables from morph-agronomic characterization and 84 polymorphic markers from rapd analysis were both separately and jointly evaluated and three dendrograms were generated. two major groups were formed in all analyses. the clusters from the joint analysis were similar to the ones from molecular analysis. one group was formed with only c. baccatum accessions and the other one with c. chinense, c. frutescens and c. annuum accessions. these results were in agreement with the gene pool complexes proposal. the association of these methods allowed a better distinction among the accessions, a cluster formation at species level and a conclusion that there is no duplicates in the collection, showing how important is the use of different methods to characterize a germplasm bank.
Meiotic irregularities in Capsicum L. species
Souza, Margarete Magalh?es;Pereira, Telma Nair Santana;Sudré, Cláudia Pombo;Rodrigues, Rosana;
Crop Breeding and Applied Biotechnology , 2012, DOI: 10.1590/S1984-70332012000200007
Abstract: cytogenetic and pollen viability (pv) studies were performed in pepper accessions, capsicum chinense and capsicum baccatum. irregularities such as laggard and univalent chromosomes, bridges, problems in the spindle fibers and cytomixis were observed, especially in c. baccatum which was the most unstable genotype. in the post-meiotic products, irregularities were observed, on average, at 20 % of the microspores in c. baccatum and 17 % in c. chinense. pv in c. baccatum was below 70 %, while in c. chinense, it was above 80 %. meiotic irregularities in capsicum, mainly in c. baccatum, considering the low pv estimated, were significant but not impeditive for fertilization.
NOTE - Meiotic irregularities in Capsicum L. species
Margarete Magalh?es Souza,Telma Nair Santana Pereira,Cláudia Pombo Sudré,Rosana Rodrigues
Crop Breeding and Applied Biotechnology , 2012,
Abstract: Cytogenetic and pollen viability (PV) studies were performed in pepper accessions, Capsicum chinense and Capsicum baccatum. Irregularities such as laggard and univalent chromosomes, bridges, problems in the spindle fibers and cytomixis were observed, especially in C. baccatum which was the most unstable genotype. In the post-meiotic products, irregularities were observed, on average, at 20 % of the microspores in C. baccatum and 17 % in C. chinense. PV in C. baccatum was below 70 %, while in C. chinense, it was above 80 %. Meiotic irregularities in Capsicum, mainly in C. baccatum, considering the low PV estimated, were significant but not impeditive for fertilization.
Genetic parameters and selection for resistance to bacterial spot in recombinant F6 lines of Capsicum annuum
Elaine Manelli Riva-Souza,Rosana Rodrigues,Cláudia Pombo Sudré,Messias Gonzaga Pereira
Crop Breeding and Applied Biotechnology , 2009,
Abstract: This study aimed to advance generations and select superior sweet pepper genotypes with resistance tobacterial spot, using the breeding method Single Seed Descent (SSD) based on the segregating population derived from thecross between Capsicum annuum L. UENF 1421 (susceptible, non-pungent) and UENF 1381 (resistant, pungent). Thesegregating F3 generation was grown in pots in a greenhouse until the F5 generation. The F6 generation was grown in fieldconditions. The reaction to bacterial spot was evaluated by inoculation with isolate ENA 4135 of Xanthomonas campestris pv.vesicatoria, based on a score scale and by calculating the area under the disease progress curve (AUDPC). The presence orabsence of capsaicin was also assessed. Eighteen F6 lines were bacterial leaf spot-resistant. Since no capsaicin was detectedin the F6 lines 032, 316, 399, 434, and 517, these will be used in the next steps of the sweet pepper breeding program.
A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.)
Moulin, Monique Moreira;Rodrigues, Rosana;Gon?alves, Leandro Sim?es Azeredo;Sudré, Cláudia Pombo;Pereira, Messias Gonzaga;
Acta Scientiarum. Agronomy , 2012, DOI: 10.4025/actasciagron.v34i2.12616
Abstract: small farmers have played an important role in maintaining genetic resources of traditional varieties. collecting and characterizing germplasm, especially landraces and heirloom varieties, are strategic activities that would create a knowledge base describing the traditional varieties of a given region and reduce the risk of genetic erosion. the objectives of this work were as follows: collect sweet potato roots from rural properties and local markets in rio de janeiro state, brazil; characterize the accessions using rapd and issr molecular markers, comparing the efficiency in discrimination of these two types of molecular markers; and estimate the genetic variability of the population. fifty-nine samples from rural properties and 19 from local markets were studied. a matrix of binary data was developed to analyze the molecular data using the jaccard index to estimate genetic differences among accessions, and clusters were made using the upgma method. the molecular techniques detected genetic variability among the accessions, with cophenetic correlation coefficients (ccc) of 0.80 for rapds and 0.89 for issrs. the rapd and issr marker results corresponded well, with a correlation of 0.55. all of the accessions were considered distinct, which demonstrates that traditional farmers maintain sweet potato genotypes that have good genetic diversity.
Agrobiodiversity in Cucurbita spp. landraces collected in Rio de Janeiro assessed by molecular markers
Santos, Marilene Hilma dos;Rodrigues, Rosana;Gon?alves, Leandro Sim?es Azeredo;Sudré, Cláudia Pombo;Pereira, Messias Gonzaga;
Crop Breeding and Applied Biotechnology , 2012, DOI: 10.1590/S1984-70332012000200001
Abstract: diversity and genetic relationship in forty landraces of cucurbita spp. collected at small farms in rio de janeiro, brazil, were analyzed by rapd and issr markers, using 20 and 15 primers, respectively. both markers were efficient to cluster the accessions separating among species, but not so much to the detection of intra-specific variability, considering the event of different pairs of accessions comprising null genetic distances observed for both markers in c. moschata. low values observed for genetic distance among the c. moschata landraces showed that most likely genetic losses is in progress in that region of cultivation due to anthropic and market pressure, which are stimulating the small farmers to abandon their local varieties in order to use commercial seeds, including hybrids, which is causing risk of genetic erosion.
Genetic divergence among Dimorphandra spp. accessions using RAPD markers
Sudré, Cláudia Pombo;Rodrigues, Rosana;Gon?alves, Leandro Sim?es Azeredo;Martins, Ernane Ronie;Pereira, Messias Gonzaga;Santos, Marilene Hilma dos;
Ciência Rural , 2011, DOI: 10.1590/S0103-84782011005000024
Abstract: the genus dimorphandra has distinguish relevance considering either medicinal or biodiversity aspects because it includes two species that are economically important flavonoids sources for pharmachemical industry (d. mollis benth. and d. gardneriana tul.), and species endemic to brazil, such as d. jorgei silva and d. wilsonii rizz., threatened by extinction. in order to evaluate variability among accessions of d. mollis (fava-d'anta), d. gardneriana and d. wilsonii, it was collected fruit from individual plants from three brazilian states in a total of 57 accessions, which were analyzed with rapd markers. it was used 20 seeds per progeny; the dna was extracted from fully-formed young leaves, which were collected in bulk. the data were analyzed using a binary matrix, in which the score one represented presence of a band and zero, absence. the similarity matrix was developed by using the arithmetic complement of the jaccard index, later grouped based on the neighbor joining algorithm. it was found considerable intra and inter specific variability in dimorphandra spp., which were separated into four groups. though genetic variability was found, the collecting trips showed that most of these areas are subject to loss of genetic resources of fava-d'anta due to the following factors: continuous anthropic activity, propensity for natural fires, and loss of natural seed dispersers (large fruit-eating mammals). therefore, protections of these areas and ex situ conservation are essentials for the maintenance of genetic variability of these species.
O sexo e o gênero da docência
Vianna, Cláudia Pereira;
Cadernos Pagu , 2002, DOI: 10.1590/S0104-83332002000100003
Abstract: in the educacional debate there is a consensus about the predominance of women in the teaching profession. however, reflections concerning the contradictory aspects that generate such a phenomenon are less frequent. the objective of this article is to discuss such aspects, that is, to examine the presence of women in the teaching profession, based on the contributions of gender and its historical, social and cultural construction. the differences between the sexes suggest that there are masculine and feminine meanings associated with teacher's identity and with school relationships; they also point to the articulation between sex and gender in teaching.
Resistência genética à mancha-bacteriana em genótipos de piment?o
Costa, Roberto Alexandre;Rodrigues, Rosana;Sudré, Cláudia Pombo;
Horticultura Brasileira , 2002, DOI: 10.1590/S0102-05362002000100016
Abstract: bacterial leaf spot (bls) is the most important bacterial disease in sweet pepper, causing intense defoliation under favorable conditions, leaving the exposed fruits to sunburn and decreasing the yield. to estimate genetic effects of the reaction to bls on leaves and fruits, under the conditions of campos dos goytacazes (brazil), hybrids f1, without reciprocals, were obtained from diallel crosses among five pepper genotypes, three susceptible ('uenf 1420 ', 'uenf 1421 ' and 'uenf 1422 ') and two resistant ('uenf 1381 ' and 'uenf 1382 '). leaves were inoculated by infiltrating the mesophyll with 103 cells/ml, adjusted with spectrophotometer. variety and mean heterosis were significant for resistance on leaves, indicating that additive and dominant effects are involved in the genetic control of this character. for resistance in fruits, only variety effects were significant, indicating the presence of additive effects in the control of this characteristic. based on these analyses, the genotypes 'uenf 1381 ' and 'uenf 1382 ' were selected for resistance to bls on leaves. for resistance to bls on fruits, 'uenf 1381' and 'uenf 1421' were the best genotypes. the best hybrids for resistance to bls on leaves were: 'uenf 1420' x 'uenf 1421', 'uenf 1382' x 'uenf 1420', 'uenf 1381' x 'uenf 1420', 'uenf 1381' x 'uenf 1421', 'uenf 1381' x 'uenf 1422' and 'uenf 1381' x 'uenf 1382'.
Resistência genética à mancha-bacteriana em genótipos de piment o
Costa Roberto Alexandre,Rodrigues Rosana,Sudré Cláudia Pombo
Horticultura Brasileira , 2002,
Abstract: A mancha-bacteriana, principal doen a bacteriana do piment o causa desfolha intensa quando em condi es favoráveis, deixando os frutos expostos ao sol, depreciando-os e diminuindo a produ o. Para estimar, nas condi es de Campos dos Goytacazes, os efeitos genéticos da rea o do hospedeiro ao patógeno, tanto em folhas como em frutos, foram obtidos híbridos F1, sem recíprocos, a partir de cruzamentos dialélicos entre cinco genótipos de piment o, sendo três suscetíveis ('UENF 1420', 'UENF 1421' e 'UENF 1422') e dois resistentes ('UENF 1381' e 'UENF 1382'). A inocula o com Xanthomonas campestris pv. vesicatoria constou de infiltra o no mesófilo foliar, utilizando-se a concentra o de 103 células/ml, ajustada com auxílio de espectrofot metro. Os efeitos de variedade e de heterose média foram significativos para resistência em folhas, indicando que efeitos aditivos e de dominancia est o envolvidos no controle genético deste caráter. Para resistência em frutos, apenas os efeitos de variedade foram significativos, indicando a presen a de efeitos aditivos no controle desta característica. A partir das análises puderam ser selecionados os parentais para RMB em folhas: 'UENF 1381' e 'UENF 1382'. Para RMB em frutos 'UENF 1381' e 'UENF 1421' foram os melhores parentais. Os melhores híbridos para RMB em folhas foram: 'UENF 1420' x 'UENF 1421', 'UENF 1382' x 'UENF 1420', 'UENF 1381' x 'UENF 1420', 'UENF 1381' x 'UENF 1421', 'UENF 1381' x 'UENF 1422' e 'UENF 1381' x 'UENF 1382'.
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