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Search Results: 1 - 10 of 13832 matches for " Ciro Martins; "
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Tuberous sclerosis complex
Rodrigues, Daniela Araujo;Gomes, Ciro Martins;Costa, Izelda Maria Carvalho;
Anais Brasileiros de Dermatologia , 2012, DOI: 10.1590/S0365-05962012000200001
Abstract: tuberous sclerosis complex, also known as epiloia or bourneville-pringle disease is an autosomal dominant neurocutaneous syndrome with variable clinical expression. it is a multisystem disorder that may be associated with hamartomas in multiple organs in an unpredictable manner. the dermatologist plays an essential role in the history of the disease, since skin manifestations represent the most prevalent clinical features, enabling early diagnosis and intervention in its natural course. this article aims to inform the scientific community about advances made in the study of genetics and molecular biology. recent findings regarding stimulation of tumor growth have been changing the history of this condition, making therapeutic trials with topical and systemic drugs possible. knowledge of these topics enables better management of the patients affected, since tissue replacement by tumors can result in significant morbidity and mortality.
Estudo de simula??es de microclimas em casas de vegeta??o visando à aclimata??o de mudas micropropagadas de bananeira cv Grande Naine
Scaranari, Ciro;Leal, Paulo Ademar Martins;Pellegrino, Giampaolo Queiroz;
Revista Brasileira de Fruticultura , 2008, DOI: 10.1590/S0100-29452008000400027
Abstract: in acclimatization processes, the environment control takes over a part of vital importance, since in in vitro condition banana seedlings do not efficiently operate the absorption of light, water and nutrients. therefore, they should be submitted to controlled environments under feasible conditions of luminosity, temperature (around 28oc, with minimum of 18oc and maximum of 34oc), and relative humidity above 75%. for this purpose, it was installed five mini tunnels with controlled temperature and relative humidity. for the control of temperature it was used pad-fan evaporative coolers at 28/25oc. for the control of relative humidity it was used fogging during the day at 75% under intermittence of 6s at each 40s. for temperature and relative humidity monitoring, it was installed three aspirate psychrometers in each mini tunnel connected to a data logger for daily acquisition every 60s. temperature and relative air humidity were adequate for plants development, however significant differences were observed between the different environment conditions. for luminosity study, under transparent plastic film (low density polyethylene) of 100μm thickness, it was used four meshes with average shading in the par spectrum band (400 to 700nm) of 69.92%, 50.73%, 29.73%, and 57.77%, being the first three in red color (with top level at 580nm, and abrupt reduction after that), the fourth mesh was black (linear behavior). the fifth tunnel had only transparent plastic film, showing 12.74% of solar radiation interception. these values were obtained from paired samples (mesh and plastic film) for the first four tunnels, and only the transparent plastic film for the last, using a spectral radiometer (400 to 1100nm), with spectral resolution of 2nm. inside and outside of each environment it was obtained global and par irradiance by using calibrated photovoltaic sensors at 9:00, 12:00, and 3:00, under clean and cloudy sky, during the summer of 2004/05, and winter of 2005. the red mesh pr
Shading and periods of acclimatization of micropropagated banana plantlets cv. Grande Naine
Scaranari, Ciro;Leal, Paulo Ademar Martins;Mazzafera, Paulo;
Scientia Agricola , 2009, DOI: 10.1590/S0103-90162009000300008
Abstract: banana plantlets obtained by micropropagation need to be submitted to a period of acclimatization since they do not use light, water, and nutrients in an efficient way. the acclimatization must be carried out under greenhouse conditions where temperature, light, and air humidity are adequate for a gradual hardening of the plantlets. in this study, the development of banana plantlets was evaluated during acclimatization under a full light condition including covered surfaces with red shade cloth (70%, 50%, and 30% shade) and black shade cloth (50% shade), both under a transparent plastic film of 100 μm. temperature, relative air humidity, irrigation, and nutrition conditions were also controlled. physical and physiological parameters were recorded at various stages in the greenhouses after three, six, and nine weeks and also after seven weeks of transplanting to field conditions. treatments were hierarchically graded according to their statistic classification. combined results indicated superior outcomes of plantlets maintained under black 50% shade cloth for nine weeks, both in the summer and winter seasons. similar results, but in a shorter time, were obtained with plantlets cultivated under red 70% shade cloth, for six weeks in the summer.
Correlation between parameters of electrophysiological, histomorphometric and sciatic functional index evaluations after rat sciatic nerve repair
Martins, Roberto Sergio;Siqueira, Mario Gilberto;Silva, Ciro Ferreira da;Plese, José Píndaro Pereira;
Arquivos de Neuro-Psiquiatria , 2006, DOI: 10.1590/S0004-282X2006000500010
Abstract: the rat sciatic nerve is a well-established model for the study of recovery from peripheral nerve injuries. traditional methods of assessing nerve regeneration after nerve injury and repair, such as electrophysiology and histomorphometry, despite widely used in neural regeneration experiments, do not necessarily correlate with return of motor and sensory functions. the aim of this experimental study is to investigate the possible correlation between several parameters of peripheral nerve regeneration after repair of sectioned sciatic nerve in wistar rat. a two-stage approach was used to obtain 17 parameters after electrophysiological, morphometric and sciatic functional index evaluations. pearson's correlation analysis was performed between these results. only two positives correlations between different classes of peripheral nerve assessments were noted, between sciatic functional index and proximal nerve fiber diameter (r=0.56, p<0.01) and between sciatic functional index and distal fiber diameter (r=0.50, p<0.01). the data presented in our study demonstrates that there is a poor correlation between the sciatic functional index and outcome measures of electrophysiological and morphometric evaluations.
Criopreserva o de ovócitos de bovinos imaturos desnudados ou n o, utilizando o etilenoglicol pelo método da vitrifica o
Costa Eduardo Paulino da,Guimar?es José Domingos,Torres Ciro Alexandre Alves,Fagundes Letícia Martins
Revista Brasileira de Zootecnia , 2002,
Abstract: Objetivou-se avaliar os efeitos da vitrifica o em ovócitos de bovinos após o cultivo in vitro, utilizando o etilenoglicol como crioprotetor. Ovócitos obtidos de ovários de vacas abatidas em matadouro foram distribuídos aleatoriamente em três tratamentos. Tratamento 0 (testemunha): ovócitos n o-desnudados e n o-congelados. Tratamento 1: vitrifica o de ovócitos imaturos n o desnudados, desidratados previamente por cinco minutos em três solu es contendo 20, 20 e 40% de etilenoglicol, acrescidas de 0,3 mol L-1 de trehalose e 20% de PVP, em meio de Talp Hepes. Tratamento 2: vitrifica o de ovócitos imaturos desnudados, conforme o Tratamento 1. Após o descongelamento (imers o em banho-maria a 30oC por 20 segundos), os ovócitos foram reidratados gradativamente, mantendo-os por 6 minutos em cada uma das solu es a seguir, sucessivamente: meio Talp Hepes com 20% de etilenoglicol + 0,3 mol L-1 de trehalose + 10% de PVP e meio Talp Hepes sem etilenoglicol, trehalose e PVP, onde foram lavados três vezes. Posteriormente, os ovócitos foram cultivados a 38,5oC, com 95% de umidade e atmosfera de 5% de CO2 por 24 horas. Após o cultivo, os ovócitos foram fecundados e os embri es cultivados in vitro por sete dias. Foi encontrada uma taxa de matura o nuclear de 81 (68/84), 19 (7/36) e 0% (0/31), nos Tratamentos 0, 1 e 2, respectivamente. As taxas de clivagem e de desenvolvimento embrionário foram de 56,4 (102/181) e 54,9% (56/102), 1,7 (1/60) e 0,0% (1/60), 0,0 (0/71) e 0,0% (0/71), nos Tratamentos 0, 1 e 2, respectivamente. Esses resultados indicam que o procedimento de vitrifica o, segundo os protocolos utilizados, n o é indicado para a criopreserva o de ovócitos de bovinos.
Criopreserva??o de ovócitos de bovinos imaturos desnudados ou n?o, utilizando o etilenoglicol pelo método da vitrifica??o
Costa, Eduardo Paulino da;Guimar?es, José Domingos;Torres, Ciro Alexandre Alves;Fagundes, Letícia Martins;Gioso, Marilú Martins;
Revista Brasileira de Zootecnia , 2002, DOI: 10.1590/S1516-35982002000500008
Abstract: the objective was to evaluate the effects of vitrification of immature bovine oocytes after in vitro culture, by the use of cryoprotectors ethylene glycol. oocytes from cows ovaries from slaughters houses were randomly alocated into three treatments. treatment 0 (control): frozen-thawed undesnude oocytes; treatment 1, immature vitrificated undesnude oocytes dehydrated for 5 minutes in each of the following solutions of 20, 20 and 40% of ethylene glycol, respectively, associated to 0.3 mol l-1 of trehalose and 20% of pvp, in media talp hepes, and, treatment 2, the same as treatment 1, but desnudes oocytes. after frozen-thawed of the oocytes (imersion in water bath at 30oc for 20 seconds), the oocytes were gradually rehydrated, in the following sequence of solutions: media talp hepes with 20% of ethylene glycol + 0.3 mol l-1 of trehalose + 10% of pvp and media talp hepes without ethylene glycol, trehalose and pvp, were washed three times. ultimately, the oocytes were cultured at 38.5oc, with 95% umidity and atmosphere of 5% of co2 for 24 hours. after culture, the oocytes were fertilized and the embryos cultured in vitro for seven days. the nuclear maturation were 81 (68/84), 19 (7/36) and 0% (0/31), for treatments 0, 1 and 2, respectively. the cleavage and development rates were: 56.4(102/181) and 54,9% (56/102), 1,7. (1/60) and 0,0% (1/60), 0,0 (0/71) and 0,0% (0/71), for the treatments 1, 2 e 3, respectively. these results show that the vitrification procedures, by the used protocols, are not indicated for bovine oocytes cryopreservation.
A importancia da endoscopia digestiva alta com solu??o de lugol no diagnóstico de cancer superficial e displasia em es?fago de doentes com neoplasias de cabe?a e pesco?o
TINCANI, Alfio José;BRANDALISE, Nelson;ANDREOLLO, Nelson Adami;LOPES, Luiz Roberto;MONTES, Ciro Garcia;ALTEMANI, Albina;MARTINS, Antonio S.;
Arquivos de Gastroenterologia , 2000, DOI: 10.1590/S0004-28032000000200007
Abstract: head and neck cancer has a high incidence in brazil, with cancer of the oral cavity being one of the five most common cancers among brazilians. alcohol and tobacco consumption may contribute to synchronous or metachronous head and neck cancer and esophageal cancer. a prospective study involving 60 patients with head and neck cancer was carried out at the state university of campinas -unicamp, campinas, sp, brazil to screen for superficial esophageal cancer and dysplasia using endoscopy and a 2% lugol dye solution followed by biopsy of the suspicious areas. five patients (8.3%) had superficial esophageal cancer, which was diagnosed as intraepithelial carcinoma in three of them (5.0%). in four patients, the superficial esophageal cancer was synchronous and in one it was metachronous to head and neck cancer. five patients (8.3%) had dysplasias in the esophageal epithelium (three were classified as mild and two as moderate). these results demonstrate the value of endoscopic screening of the esophagus using lugol dye in patients with head and neck cancer, particularly since superficial esophageal cancer is extremely difficult to detect by conventional methods in asymptomatic patients.
Electrophysiologic assessment of regeneration in rat sciatic nerve repair using suture, fibrin glue or a combination of both techniques
Martins, Roberto Sergio;Siqueira, Mario Gilberto;Silva, Ciro Ferreira da;Godoy, Benedito Ortiz de;Plese, José Píndaro Pereira;
Arquivos de Neuro-Psiquiatria , 2005, DOI: 10.1590/S0004-282X2005000400009
Abstract: we evaluated the repair of seccioned rat sciatic nerve by the comparison of electrophysiologic parameters. the repair was effected with suture (group a), fibrin glue (group b) or a combination of both techniques (group c). the amplitude, latency and conduction velocity of the motor and nerve action potentials were assessed before the nerve section and at reoperation after 24 weeks. there was no difference between the groups when the nerve action potential was evaluated. rats of group b presented better results than those of group a (p<0.05) when latency and the nerve conduction velocity assessed at the reoperation, and the ratio between the conduction velocity at the reoperation and before the nerve section in the motor action potential evaluation were measured. animals of group c presented better results than those of group a when the ratio between the conduction velocity of motor action potential at the reoperation and before the nerve division was considered (p<0.05). no difference between groups b and c was found. we conclude that repair using fibrin glue presented better results than suture following transection of sciatic nerve when the motor action potential was evaluated in the rat experimental model.
Vitrifica??o de ovócitos desnudados ou n?o e previamente maturados in vitro
Fagundes, Letícia Martins;Costa, Eduardo Paulino da;Torres, Ciro Alexandre Alves;Amaral Filha, Wald'ma Sobrinho;Silva, Trícia Osório da;Gioso, Marilú Martins;
Revista Brasileira de Zootecnia , 2004, DOI: 10.1590/S1516-35982004000500004
Abstract: this study aimed at the evaluation of the effects from cryopreservation of bovine oocytes in vitro matured, by using ethylene glycol (eg) associated to trehalose and polyvinylpyrrolidone (pvp), of ovary oocytes of slaughtered cows, randomly assigned to three treatments. treatment 0 (t0 - control): oocytes that were desnuded and not vitrified. treatment 1 (t1): cryopreservation of in vitro matured oocytes with cumulus oophorus. tratamento 2 (t2): cryopreservation of in vitro matured desnuded oocytes. the percentage of recovered oocytes after cryopreservation and with normal morphology was different for vitrified oocytes (94.7 and 76.8%; 69.5 and 48.85% for t1 and t2, respectively). the main changes ultrastructural in vitrificated oocytes were prematurely released of cortical granules. later, all normal oocytes were fecundated and cultivated at 38.5oc in atmosphere with 5% co2 for seven days. the fecundation and cleavage rates for treatments were different (56.2, 41.7 and 12.5%; 36.3, 0.0 and 0.0%, for t0, t1 and t2, respectively). morulas and blastocysts were obtained only in t0 (34.5%). these results indicate that, the used protocols, for vitrification procedure is not indicated for cryopreservation of matured bovine oocytes.
Resistencia a la enfermedad de cría yesificada por colonias de Apis mellifera con eficiente comportamiento higiénico (Hymenoptera, Apidae)
Invernizzi, Ciro;
Iheringia. Série Zoologia , 2001, DOI: 10.1590/S0073-47212001000200016
Abstract: in an apiary composed of 14 hygienic and 7 non-hygienic colonies of apis mellifera linnaeus, 1758 the presence of visible and capped mummies was recorded, one hygienic and 4 non-hygienic colonies showed symptoms of chalkbrood. twenty-eight days after a massive contamination of the colonies with pollen patties containing ascosphaera apis olive & spiltoir, 1955, the situation was almost identical to that at the beginning: the same 4 non-hygienic colonies still were infected and one hygienic colony that was healthy became infected. the high proportion of hygienic colonies that eliminated the disease symptoms suggests that they could maintain themselves healthy in spite of the presence of colonies with chalkbrood in the apiary.
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