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Search Results: 1 - 10 of 6540 matches for " Chunxue Bai "
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Respiratory diseases call for special attention from clinical and translational science
Chunxue Bai
Translational Respiratory Medicine , 2013, DOI: 10.1186/2213-0802-1-1
Abstract: Respiratory diseases will become one of the top 3 leading causes of estimated mortality in 2020 and become about one third of total causes of estimated mortality. The journal of Translational Respiratory Medicine is a truly international, peer-reviewed journal devoted to the publication of articles on outstanding work with translational potentials between basic research and clinical application to understanding respiratory disease. Translational respiratory medicine will more focus on biomarker identification and validation in pulmonary diseases in combination with clinical informatics, targeted proteomics, bioinformatics, systems medicine, or mathematical science; on different translational strategies of cell-based therapy to clinical application to treat lung diseases; on targeted therapies in combination with personalized medicine; and on distant electronic medicine to monitor a large population of people’s health. Translational Respiratory Medicine is an additional but unique opportunity for scientists and clinicians who work on pulmonary diseases to publish their outstanding findings, initiative results, and critical and perceptive opinions in the journal.
Bioinformatics insights into acute lung injury/acute respiratory distress syndrome
Xiaocong Fang, Chunxue Bai, Xiangdong Wang
Clinical and Translational Medicine , 2012, DOI: 10.1186/2001-1326-1-9
Abstract: In recent years, there is a great increase in genomics and other molecular research which produced a tremendous amount of information related to molecular biology [1-3]. Meantime, various powerful data mining and statistical bioinformatics methods have been propagated for identifying, prioritizing and classifying robust and generalizable biomarkers with high discriminatory ability [4], and some online bioinformatics data libraries, such as Enzyme, KEGG, Gene Ontology, NCBI Taxonomy, SwissProt and TrEMBL were generated for the store and management of data. Bioinformatics now entails the creation and advancement of databases, algorithms, computational and statistical techniques and theory to solve formal and practical problems arising from the management and analysis of biological data.Clinical bioinformatics is a new emerging science combining clinical information, (including patients’ complaints, history, clinical symptoms and signs, physicians’ examinations, biochemical analysis, imaging profiles, pathologies), omics science, mathematics, information technology and library data together. It may play a potentially important role in the discovery of biomarkers, which will facilitate the diagnosis of disease, as well as treatment. A commonly used definition of a biomarker is “a characteristic that is objectively measured and evaluated as an indicator of normal biological process, pathogenic processes, or pharmacologic responses to a therapeutic intervention” [5]. Clinical bioinformatics is also a new way to focus on the combination of clinical symptoms and signs with human tissue-generated bioinformatics to get deep and full understand of the risk factors, pathogenesis and progress of human disease. Recent technological developments in genomics, proteomics, metabolomics, are allowing researchers to take an unbiased ‘big-picture’ approach to further elucidate the molecular pathogenesis of lung injury [6] (Figure 1). Recent years, clinical bioinformatics have been widel
Characteristics of Chinese patients with cough in primary care centre
Qunying Hong, Chunxue Bai, Xiangdong Wang
Journal of Translational Medicine , 2011, DOI: 10.1186/1479-5876-9-149
Abstract: Approximately 18,000 subjects recruited were having daytime or night symptoms of cough and diagnoses of respiratory disease from February 2005 to April 2006 as Survey 1 and from June 2007 to December 2007 as Survey 2. Patients suffering from respiratory malignancy, hyperthyroidism, hypertension, heart disease, diabetes, severe hypohepatia or renal dysfunction, pregnancy, possible pregnancy or lactation, neutropenia were not eligible. Information regarding demography, history of allergies, symptomatic profile, treatment and curative effects for cough was elicited.8216 questionnaires were collected in Survey 1 and 9711 in Survey 2. The mean values of ages were 25.7 and 22.3 years old, respectively. Symptoms included expectoration (74% and 76%), wheeze (59% and 74%), breathlessness (22% and 26%), chest pain (9% and 13%) and fever (15% and 18%). About 15% and 23% patients had hypersusceptibility, of whom 6% to 17% had a family history. More than 50% of the cases had histories of allergic rhinitis, asthma, conjunctivitis or atopic dermatitis. Asthma, COPD, and bronchitis were dominant etiologies of cough. Procaterol or the combination of antibiotics and steroids were used as the treatment.Causes and outcomes of cough differed with ages and time in this particular national study, while successful and precise diagnosis and management of cough in primary care settings need to be further improved in China.Cough is one of the most common respiratory symptoms encountered by clinicians [1], with the reported prevalence varying between 5% and 40% [2-4]. Cough is also one of the critical factors affecting the quality of life in patients with respiratory diseases. Cough management has massive economic consequences. The first guideline on the management of cough with a significant positive impact was championed in 1998 [5], followed by the publication of other guidelines on chronic cough [6-9]. The Chinese Medical Association published "Diagnosis and treatment guide (draft) of coug
The comparison of the efficacy of swine FMD vaccine emulsified with oil adjuvant of ISA 201 VG or ISA 206 VG  [PDF]
Dong Li, Chunxue Zhou, Daliang She, Pinghua Li, Pu Sun, Xingwen Bai, Yingli Chen, Baoxia Xie, Zaixin Liu
Journal of Biosciences and Medicines (JBM) , 2013, DOI: 10.4236/jbm.2013.13005

The Seppic Company developed a new adjuvant Montanide ISA 201 VG, the upgraded version of Montanide ISA 206 VG, which keep the advantage and added some chemical components on the basis of ISA 206 to improve the cellular responses. The aim of the study is to compare the efficacy of swine FMD (foot-and-mouth) vaccine emulsified with oil adjuvant of ISA 201 or ISA 206 respectively. The pigs were vaccinated with FMD vaccine emulsified with inactive FMD type O antigen and adjuvant ISA 201 or ISA 206 respectively, according to 2.0 ml (1/1 dose), 0.67 ml (1/3 dose), 0.22 ml (1/9 dose) to calculate their PD50. The sera were collected from the vaccination of the day 0, 3, 7, 14, 21, 28 and the ELISA FMD type O antibody were detected. Furthermore, the PD50 were calculated after the pigs were challenged with virulent FMDV type O on 28 days post vaccination. The ELISA antibody titers of 201vaccine were significantly higher than that of 206 (except the third time). The fifty percent of protection dose (PD50) of 201 vaccine (PD50 = 15.59) was higher than that of 206 vaccine (PD50 = 10.05). The above data showed that the efficacy of the FMD vaccine emulsified with ISA 201 was better than which with ISA 206.

Protection against the allergic airway inflammation depends on the modulation of spleen dendritic cell function and induction of regulatory T cells in mice
Wang Yaoli,Bai Chunxue,Wang Guansong,Wang Diane
Genetic Vaccines and Therapy , 2010, DOI: 10.1186/1479-0556-8-2
Abstract: Background Allergen-induced imbalance of specific T regulatory (Treg) cells and T helper 2 cells plays a decisive role in the development of immune response against allergens. Objective To evaluate effects and potential mechanisms of DNA vaccine containing ovalbumin (OVA) and Fc fusion on allergic airway inflammation. Methods Bronchoalveolar lavage (BAL) levels of inflammatory mediators and leukocyte infiltration, expression of CD11c+CD80+ and CD11c+CD86+ co-stimulatory molecules in spleen dendritic cells (DCs), circulating CD4+ and CD8+ T cells, Foxp3+ in spleen CD4+ T cells and spleen CD4+ T cells were measured in OVA-sensitized and challenged animals pretreated with pcDNA, OVA-pcDNA, Fc-pcDNA, and OVA-Fc-pcDNA. Results OVA-Sensitized and challenged mice developed airway inflammation and Th2 responses, and decreased the proliferation of peripheral CD4+and CD8+ T cells and the number of spleen Foxp3+ Treg. Those changes with increased INF-γ production and reduced OVA-specific IgE production were protected by the pretreatment with OVA-Fc-pcDNA. Conclusion DNA vaccine encoding both Fc and OVA showed more effective than DNA vaccine encoding Fc or OVA alone, through the balance of DCs and Treg.
A short-term educational program improved physicians’ adherence to guidelines for COPD and asthma in Shanghai
Xiaocong Fang, Shanqun Li, Lei Gao, Naiqing Zhao, Xiangdong Wang, Chunxue Bai
Clinical and Translational Medicine , 2012, DOI: 10.1186/2001-1326-1-13
Abstract: A prescription survey was performed in a random sample of 100 COPD and asthma outpatients to assess their pharmacological therapy. Then, an educational program was conducted in young pulmonary physicians from 83 hospitals in Shanghai. The training course was divided into 7 sessions of 2?hours delivered over 4?days from July 2010 to August 2011. Three months later, all of the participants were asked to take a written examination to assess the efficiency of training.Prescription survey among the patients indicated the prescriptions are not consistent with the recommendations of current GOLD and GINA guidelines. The mainly existing issue is the overuse of inhaled glucocorticosteroid. For the educational program, 161 pulmonary physicians have attended the training course, and 110 clinicians finished the tests with an attendance rate of 68.3%. Although most of the clinicians recognized the increasing burden of COPD and asthma, they do not know well about the core elements of guidelines and their clinical practice is not fully in agreement with current recommendations. Through crossover comparison, our results suggested clinicians’ knowledge of the guidelines was improved after training.We concluded that application of continuous educational programs among physicians might promote their adherence to guidelines, and by that improve the quality of healthcare.Chronic obstructive pulmonary disease (COPD) and asthma are both chronic respiratory diseases characterized by the impairment of lung function, and they are also complex multi-component diseases accompanied with mental and physical co-morbidities [1,2]. In recent years, there has been increasing evidences suggesting that COPD and asthma are imposing enormous burden on patients, healthcare professionals and society in terms of morbidity, mortality, healthcare resources utilization and expense worldwide [3], especially in developing countries [4-9].Despite these striking statistics, the management of COPD and asthma is fa
Downregulation of miR-17~92 Expression Increase Paclitaxel Sensitivity in Human Ovarian Carcinoma SKOV3-TR30 Cells via BIM Instead of PTEN
Ting Shuang,Chunxue Shi,Shuang Chang,Min Wang,Cui Hong Bai
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms14023802
Abstract: To better understand the molecular mechanisms of paclitaxel resistance in ovarian carcinoma, we evaluated the expression of miRNAs using miRNA microarray between human ovarian carcinoma SKOV3 cells and paclitaxel resistant SKOV3-TR30 cells. Results showed that 69 miRNAs were upregulated while 102 miRNAs were downregulated in SKOV3-TR30 cells. Using real-time PCR, we further clarified that miR-17~92 was overexpressed in SKOV3-TR30 cells compared with that in SKOV3 cells. We then established stable virally transduced SKOV3-TR30-m-PTIP-Sponge all SKOV3-TR30 cells and its vector-only control SKOV3-TR30-m-PTIP-GFP cells. Real time-PCR revealed that SKOV3-TR30-m-PTIP-Sponge all cells expressed approximately 6.18-fold lower levels of miR-17~92 compared with the control group. Decreased expression of miR-17~92 resulted in cell cycle arrest in the G2/M phase and growth inhibition. After the transduction, the BIM protein level was increased in SKOV3-TR30 cells and luciferase reporter assays revealed that miR-17~92 binds directly to the 3'-UTR of BIM. Results of luciferase reporter assays accompanied with Western Blot showed that although miR-17~92 binds directly to the 3'-UTR of PTEN, the PTEN protein expression level was upregulated slightly while the result is of no statistical significance. Our results showed that miR-17~92 could be a causal factor of the downregulation of BIM in SKOV3-TR30 cells and thus induce the paclitaxel resistance in SKOV3-TR30 cells.
Genetic variants in the TIRAP gene are associated with increased risk of sepsis-associated acute lung injury
Zhenju Song, Chaoyang Tong, Zhan Sun, Yao Shen, Chenling Yao, Jinjun Jiang, Jun Yin, Lei Gao, Yuanlin Song, Chunxue Bai
BMC Medical Genetics , 2010, DOI: 10.1186/1471-2350-11-168
Abstract: A case-control collection from Han Chinese of 298 healthy subjects, 278 sepsis-associated ALI and 288 sepsis alone patients were included. Three tag single nucleotide polymorphisms (SNPs) of the TIRAP gene and two additional SNPs that have previously showed association with susceptibility to other inflammatory diseases were genotyped by direct sequencing. The differences of allele, genotype and haplotype frequencies were evaluated between three groups.The minor allele frequencies of both rs595209 and rs8177375 were significantly increased in ALI patients compared with both healthy subjects (odds ratio (OR) = 1.47, 95% confidence interval (CI):1.15-1.88, P = 0.0027 and OR = 1.97, 95% CI: (1.38-2.80), P = 0.0001, respectively) and sepsis alone patients (OR = 1.44, 95% CI: 1.12-1.85, P = 0.0041 and OR = 1.82, 95% CI: 1.28-2.57, P = 0.00079, respectively). Haplotype consisting of these two associated SNPs strengthened the association with ALI susceptibility. The frequency of haplotype AG (rs595209A, rs8177375G) in the ALI samples was significantly higher than that in the healthy control group (OR = 2.13, 95% CI: 1.46-3.09, P = 0.00006) and the sepsis alone group (OR = 2.24, 95% CI: 1.52-3.29, P = 0.00003). Carriers of the haplotype CA (rs595209C, rs8177375A) had a lower risk for ALI compared with healthy control group (OR = 0.69, 95% CI: 0.54-0.88, P = 0.0003) and sepsis alone group (OR = 0.71, 95% CI: 0.55-0.91, P = 0.0006). These associations remained significant after adjustment for covariates in multiple logistic regression analysis and for multiple comparisons.These results indicated that genetic variants in the TIRAP gene might be associated with susceptibility to sepsis-associated ALI in Han Chinese population. However, the association needs to be replicated in independent studies.Acute lung injury (ALI) and its more severe form, the acute respiratory distress syndrome (ARDS), are syndromes of acute respiratory failure that are characterized by acute pulmonary ed
A linear polyethylenimine mediated siRNA-based therapy targeting human epidermal growth factor receptor in SPC-A1 xenograft mice
Pinghai Zhang , Nuo Xu , Lei Zhou, Xin Xu, Yuehong Wang, Ka Li, Zhaochong Zeng, Xiangdong Wang, Xin Zhang and Chunxue Bai
Translational Respiratory Medicine , 2013, DOI: 10.1186/2213-0802-1-2
Abstract: The novel modality of siRNA-based therapy targeting EGFR may be of great potential in NSCLC treatment.
Dexamethasone Reduces Sensitivity to Cisplatin by Blunting p53-Dependent Cellular Senescence in Non-Small Cell Lung Cancer
Haiyan Ge, Songshi Ni, Xingan Wang, Nuo Xu, Ying Liu, Xun Wang, Lingyan Wang, Dongli Song, Yuanlin Song, Chunxue Bai
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0051821
Abstract: Introduction Dexamethasone (DEX) co-treatment has proved beneficial in NSCLC patients, improving clinical symptoms by the reduction of side effects after chemotherapy. However, recent studies have shown that DEX could render cancer cells more insensitive to cytotoxic drug therapy, but it is not known whether DEX co-treatment could influence therapy-induced senescence (TIS), and unknown whether it is in a p53-dependent or p53-independent manner. Methods We examined in different human NSCLC cell lines and detected cellular senescence after cisplatin (DDP) treatment in the presence or absence of DEX. The in vivo effect of the combination of DEX and DDP was assessed by tumor growth experiments using human lung cancer cell lines growing as xenograft tumors in nude mice. Results Co-treatment with DEX during chemotherapy in NSCLC resulted in increased tumor cell viability and inhibition of TIS compared with DDP treated group. DEX co-treatment cells exhibited the decrease of DNA damage signaling pathway proteins, the lower expression of p53 and p21CIP1, the lower cellular secretory program and down-regulation of NF-κB and its signaling cascade. DEX also significantly reduced DDP sensitivity in vivo. Conclusions Our results underscore that DEX reduces chemotherapy sensitivity by blunting therapy induced cellular senescence after chemotherapy in NSCLC, which may, at least in part, in a p53-dependent manner. These data therefore raise concerns about the widespread combined use of gluocorticoids (GCs) with antineoplastic drugs in the clinical management of cancer patients.
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