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OALib Journal期刊

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α-Galactosyl Phytosphingosine 2,6’-Diamide as an Inducer of Invariant Natural Killer T Cell  [PDF]
Ying-Cheng Huang, Wei-Ting Chen, Shu-Fan Tien, Ho-Lien Huang, Chun-Nan Yeh, Kun-I Lin, Chung-Shan Yu
Open Journal of Medicinal Chemistry (OJMC) , 2013, DOI: 10.4236/ojmc.2013.33008
Abstract:

Four a-galactosyl phytosphingosine 2,6’-diamide analogs were prepared from 2,6’-diamino a-galactosylphytosphingosine and the aromatic-bearing carboxylic acids. After purification with High Performance Liquid Chromatography, a flowcytometry for the four compounds for stimulation of human Va24+/Vb11+ NKT cell populations was carried out. Additional keto groups on the acyl chains of the 2,6’-diamide compound was associated with the enhanced stimulating effect.


The Application of Structural Holes Theory to Supply Chain Network Information Flow Analysis
Chun-Nan Lin,Kun-Lin Hsieh,Jinsheng Roan,Chuan-Hung Kao
Information Technology Journal , 2011,
Abstract: This study used Honda supply chain as an illustrative case based on the social network perspective. We firstly examined the extent of structural holes of the chain members and then applied a heuristic approach to alleviate structural holes effects. Two important results were obtained as follows: (1) Many structural holes during the network structure for Honda supply chain because those members do not have many connections with each other. (2) A heuristic based on bridge-building concept was proposed to effectively resolve the structural holes problem of Honda supply chain.
Comparative Analysis of Production Costs and Revenue on the Banana Planting Season in Taiwan  [PDF]
Te-Sheng Lin, Chun-Nan Lin, Ke-Chung Peng
Open Access Library Journal (OALib Journal) , 2019, DOI: 10.4236/oalib.1105180
Abstract:
The planting season of crops has always been an important research topic to the revenue. This research aims to use bananas as a case study and use the principle of break-even analysis to explore the issues of profit and loss and market potential of the spring-summer’s bananas and the autumn-winter’s bananas in Taiwan. The secondary data were collected from Council of Ag-riculture, Executive Yuan Taiwan during 2011-2015. The concept of break-even analysis was applied in this research as the research methodol-ogy. The empirical results found that the break-even points of spring-summer’s bananas and autumn-winter’s bananas during 2010-2015 were decreasing due to the increase of an average market selling price. However, the spring-summer’s bananas have a higher revenue ratio than the other season. Therefore, the bananas’ growers in Taiwan should plant ba-nanas during the spring-summer season in order to gain a higher profit.
Sequence features involved in the mechanism of 3' splice junction wobbling
Kuo-Wang Tsai, Wen-Ching Chan, Chun-Nan Hsu, Wen-chang Lin
BMC Molecular Biology , 2010, DOI: 10.1186/1471-2199-11-34
Abstract: By browsing the Alternative Splicing Database information, we observed that most 3' alternative splice site choices occur within six nucleotides of the dominant splice site and the incidence significantly decreases further away from the dominant acceptor site. Although a lower frequency of alternative splicing occurs within the intronic region (alternative splicing at the proximal AG) than in the exonic region (alternative splicing at the distal AG), alternative AG sites located within the intronic region show stronger potential as the acceptor. These observations revealed that the choice of 3' splice sites during 3' splicing junction wobbling could depend on the distance between the duplicated AG and the branch point site (BPS). Further mutagenesis experiments demonstrated that the distance of AG-to-AG and BPS-to-AG can greatly influence 3' splice site selection. Knocking down a known alternative splicing regulator, hSlu7, failed to affect wobble splicing choices.Our results implied that nucleotide distance between proximal and distal AG sites has an important regulatory function. In this study, we showed that occurrence of 3' wobble splicing occurs in a distance-dependent manner and that most of this wobble splicing is probably caused by steric hindrance from a factor bound at the neighboring tandem motif sequence.Alternative splicing is an important mechanism of gene regulation in the human genome, occurring in around 40-60% of human genes [1]. The 5' and 3' alternative splicing events contribute 25% of all alternative splicing instances and such events often result in frameshift mutations or insertion/deletion of amino acids in the expressed proteins [2]. Recent studies indicated that certain alternative 5'/3' tandem splice site selections are only a few nucleotides apart, and that such short nucleotide length variations can still lead to subtle changes in protein structure through the modification of coding amino acids [3-5]. Interestingly, this phenomenon occu
Characterization of a novel rat cholangiocarcinoma cell culture model-CGCCA
Chun-Nan Yeh,Kun-Ju Lin,Tsung-Wen Chen,Ren-Ching Wu
World Journal of Gastroenterology , 2011, DOI: 10.3748/wjg.v17.i24.2924
Abstract: AIM: To characterize a culture model of rat CCA cells, which were derived from a transplantable TTA-induced CCA and designated as Chang Gung CCA (CGCCA). METHODS: The CGCCA cells were cultured at in vitro passage 12 times on a culture dish in DMEM medium. To measure the doubling time, 103 cells were plated in a 96-well plate containing the growth medium. The cells were harvested 4 to 10 d after seeding, and a standard MTT assay was used to measure the growth. The phenotype of CACCA cell and xenograft was determined by immunohistochemical study. We also determine the chromosomal alterations of CGCCA, G-banding and spectral karyotyping studies were performed. The CGCCA cell line was transplanted into the nude mice for examining its tumorigenicity. 2-Deoxy-2-(18F)fluoro-D-glucose (FDG) autoradiography was also performed to evaluate the FDG uptake of the tumor xenograft. RESULTS: The doubling time for the CGCCA cell line was 32 h. After transplantation into nude mice, FDG autoradiography showed that the tumors formed at the cell transplantation site had a latency period of 4-6 wk with high FDG uptake excluding necrosis tissue. Moreover, immunohistochemical staining revealed prominent cytoplasmic expression of c-erb-B2, CK19, c-Met, COX-II, EGFR, MUC4, and a negative expression of K-ras. All data confirmed the phenotypic features of the CGCCA cell line coincide with the xenograft mice tumors, indicating cells containing the tumorigenicity of CCA originated from CCA. In addition, karyotypic banding analysis showed that the diploid (2n) cell status combines with ring and giant rod marker chromosomes in these clones; either both types simultaneously appeared or only one type of marker chromosome in a pair appeared in a cell. The major materials contained in the marker chromosome were primarily identified from chromosome 4. CONCLUSION: The current CGCCA cell line may be used as a non-K-ras effect CCA model and to obtain information and reveal novel pathways for CCA. Further applications regarding tumor markers or therapeutic targeting of CCA should be addressed accordingly.
No Increased Risk of Herpes Zoster Found in Cirrhotic Patients: A Nationwide Population-Based Study in Taiwan
Ping-Hsun Wu, Yi-Ting Lin, Chun-Nan Kuo, Wei-Chiao Chang, Wei-Pin Chang
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0093443
Abstract: Background The association between liver cirrhosis (LC) and herpes zoster has rarely been studied. We investigated the hypothesis that LC, known as an immunodeficiency disease, may increase the risk of herpes zoster using a national health insurance database in Taiwan. Materials and Methods The study cohort included cirrhotic patients between 1998 and 2005 (n = 4667), and a ratio of 1:5 randomly sampled age- and gender-matched control patients (n = 23,335). All subjects were followed up for 5 years from the date of cohort entry to identify whether or not they had developed herpes zoster. Cox proportional-hazard regressions were performed to evaluate 5-year herpes zoster-free survival rates. Results Of all patients, 523 patients developed herpes zoster during the 5-year follow-up period, among whom 82 were LC patients and 441 were in the comparison cohort. The adjusted hazard ratio (AHR) of herpes zoster in patients with LC was not higher (AHR: 0.77, 95% confidence interval: 0.59–1.01, p = 0.06) than that of the controls during the 5-year follow-up. No increased risk of herpes zoster was found in LC patients after stratification by age, gender, urbanization level, income, geographic region, and all comorbidities. Conclusions This large nationwide population-based cohort study suggests that there is no increased risk for herpes zoster among people who have LC compared to a matching population.
Antiplatelet Effect and Selective Binding to Cyclooxygenase (COX) by Molecular Docking Analysis of Flavonoids and Lignans
Chien-Ming Wu,Shu-Chun Wu,Wan-Jung Chung,Hsien-Cheng Lin,Kun-Tze Chen,Yu-Chian Chen,Mei-Feng Hsu,Jwu-Maw Yang,Jih-Pyang Wang,Chun-Nan Lin
International Journal of Molecular Sciences , 2007, DOI: 10.3390/i8080830
Abstract: The known flavonoids ginkgetin (1), taiwanhomoflavone A (2),taiwanhomoflavone B (3), and taiwanhomoflavone C (4) and eight known lignans:justicidin B (9), justicidin C (10), justicidin D (11), chinensinaphthol methyl ether (12),procumphthalide A (13), procumbenoside A (15), and ciliatosides A (16) and B (17) wereisolated from Cephalotaxus wilsoniana and Justicia species, respectively. The antiplateleteffects of the above constituents on human platelet-rich plasma (PRP) were evaluated. Ofthe compounds tested on human PRP, compounds 1, 4, 9, and 11 showed inhibition ofsecondary aggregation induced by adrenaline. Compound 1 had an inhibitory effect oncyclooxygenase-1 (COX-1). Molecular docking studies revealed that 1 and the related compounds apigenin (5), cycloheterophyllin (6), broussoflavone F (7), and quercetin (8) were docked near the gate of active site of COX-1. It indicated that the antiplatelet effect of 1, 4, 9, and 11 is partially owed to suppression of COX-1 activity and reduced thromboxane formation. Flavonoids, 1, 5, 6, 7, and 8 may block the gate of the active site of COX-1 and interfere the conversion of arachidonic acid to prostaglandin (PG) H2 in the COX-1 active site.
Local anesthesia with ropivacaine for patients undergoing laparoscopic cholecystectomy
Yu-Yin Liu, Chun-Nan Yeh, Hsiang-Lin Lee, Shang-Yu Wang, Chun-Yi Tsai, Chih-Chung Lin, Tzu-Chieh Chao, Ta-Sen Yeh, Yi-Yin Jan
World Journal of Gastroenterology , 2009,
Abstract: AIM: To investigate the effect of pain relief after infusion of ropivacaine at port sites at the end of surgery.METHODS: From October 2006 to September 2007, 72 patients undergoing laparoscopic cholecystectomy (LC) were randomized into two groups of 36 patients. One group received ropivacaine infusion at the port sites at the end of LC and the other received normal saline. A visual analog scale was used to assess postoperative pain when the patient awakened in the operating room, 6 and 24 h after surgery, and before discharge. The amount of analgesics use was also recorded. The demographics, laboratory data, hospital stay, and perioperative complications were compared between the two groups.RESULTS: There was no difference between the two groups preoperatively in terms of demographic and laboratory data. After surgery, similar operation time, blood loss, and no postoperative morbidity and mortality were observed in the two groups. However, a significantly lower pain score was observed in the patients undergoing LC with local anesthesia infusion at 1 h after LC and at discharge. Regarding analgesic use, the amount of meperidine used 1 h after LC and the total used during admission were lower in patients undergoing LC with local anesthesia infusion. This group also had a shorter hospital stay.CONCLUSION: Local anesthesia with ropivacaine at the port site in LC patients significantly decreased postoperative pain immediately. This explains the lower meperidine use and earlier discharge for these patients.
Liver angiosarcoma, a rare liver malignancy, presented with intraabdominal bleeding due to rupture- a case report
Chin-Ying Chien, Cheng-cheng Hwang, Chun-nan Yeh, Huang-yang Chen, Jui-Teng Wu, Siu-Cheung Chan, Chih-Lang Lin, Cho-li Yen, Wen-yen Wang, Kun-Chun Chiang
World Journal of Surgical Oncology , 2012, DOI: 10.1186/1477-7819-10-23
Abstract: Hepatic angiosarcoma is a very rare disease, accounting for only 2% of primary liver malignancy [1-3]; however, it still ranks as the third place in the list of most common primary liver malignancies [2,3]. Hepatic angiosarcoma originates from endothelial cells and usually presents as an abdominal mass with unspecific symptoms and signs [4], making it difficult to diagnose in the early stage. The survival of hepatic angiosarcoma is very poor, which is attributable to its rapid progress, high recurrence rate, and resistant to traditional chemotherapy and radiotherapy [5-7]. Even liver transplantation could not benefit patients with liver angiosarcoma [8]. To date, the therapeutic guideline for liver angiosarcoma has not been set up; partial liver resection to remove tumor radically still remains to be the cornerstone of treatment options. Here we reported a case with primary liver angiosarcoma presenting with hemoperitoneum due to tumor rupture and active bleeding treated by TAE and subsequent partial liver resection.An 83 year-old female complained about lower abdomen pain for two months. After one episode of fierce right upper abdomen pain occurred on July 12th, 2011, she was brought to our emergency department for aid by her families. In our emergency department (ER), unstable systolic blood pressure of 90 mmHg with tachycardia of 110 beats/min was found. Resuscitation was then given. Subsequent blood examination showed leukocytosis (161000/μL), anemia (9 g/dL), and thrombocytopenia (85000/μL). Abdomen distension with mild tenderness while pressed was also noted. Under the impression of internal bleeding, bedside echo was then conducted, which revealed intra-abdomen fluid accumulation and a liver mass. Abdomen computer tomography(CT) was thereafter arranged for this patient with contrast injection, which indicated a 3 cm hypervascular mass over segment 5 of liver with active bleeding (Figure 1). An emergent angiogram of celiac trunk was then conducted and showed a
Reappraisal of the Therapeutic Role of Celecoxib in Cholangiocarcinoma
Chun-Nan Yeh, Kun-Chun Chiang, Horng-Heng Juang, Jong-Hwei S. Pang, Chung-Shan Yu, Kun-Ju Lin, Ta-Sen Yeh, Yi-Yin Jan
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0069928
Abstract: Cholangiocarcinoma (CCA), a lethal disease, affects many thousands worldwide yearly. Surgical resection provides the best chance for a cure; however, only one-third of CCA patients present with a resectable tumour at the time of diagnosis. Currently, no effective chemotherapy is available for advanced CCA. Cyclooxygenase-2 (COX-2) is a potential oncogene expressing in human CCA tissues and represents a candidate target for treatment; however, COX-2 inhibitors increase the risk of negative cardiovascular events as application for chemoprevention aim. Here, we re-evaluated the effectiveness and safety of celecoxib, one widely used COX-2 inhibitor, in treating CCA. We demonstrated that celecoxib exhibited an anti-proliferative effect on CGCCA cells via cell cycle arrest at G2 phase and apoptosis induction. Treatment for 5 weeks high dose celecoxib (160 mg/kg) significantly repressed thioacetamide-induced CCA tumour growth in rats as monitored by animal positron emission tomography through apoptosis induction. No obviously observable side effects were noted during the therapeutic period. As retrospectively reviewing 78 intrahepatic mass-forming CCA patients, their survival was strongly and negatively associated with a positive resection margin and high COX-2 expression. Based on our result, we concluded that short-term high dose celecoxib may be a promising therapeutic regimen for CCA. Yet its clinical application still needs more studies to prove its safety.
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