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Search Results: 1 - 10 of 212553 matches for " Christine L. Mummery "
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Human Embryonic and Fetal Mesenchymal Stem Cells Differentiate toward Three Different Cardiac Lineages in Contrast to Their Adult Counterparts
Arti A. Ramkisoensing, Dani?l A. Pijnappels, Sa?d F. A. Askar, Robert Passier, Jim Swildens, Marie José Goumans, Cindy I. Schutte, Antoine A. F. de Vries, Sicco Scherjon, Christine L. Mummery, Martin J. Schalij, Douwe E. Atsma
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0024164
Abstract: Mesenchymal stem cells (MSCs) show unexplained differences in differentiation potential. In this study, differentiation of human (h) MSCs derived from embryonic, fetal and adult sources toward cardiomyocytes, endothelial and smooth muscle cells was investigated. Labeled hMSCs derived from embryonic stem cells (hESC-MSCs), fetal umbilical cord, bone marrow, amniotic membrane and adult bone marrow and adipose tissue were co-cultured with neonatal rat cardiomyocytes (nrCMCs) or cardiac fibroblasts (nrCFBs) for 10 days, and also cultured under angiogenic conditions. Cardiomyogenesis was assessed by human-specific immunocytological analysis, whole-cell current-clamp recordings, human-specific qRT-PCR and optical mapping. After co-culture with nrCMCs, significantly more hESC-MSCs than fetal hMSCs stained positive for α-actinin, whereas adult hMSCs stained negative. Furthermore, functional cardiomyogenic differentiation, based on action potential recordings, was shown to occur, but not in adult hMSCs. Of all sources, hESC-MSCs expressed most cardiac-specific genes. hESC-MSCs and fetal hMSCs contained significantly higher basal levels of connexin43 than adult hMSCs and co-culture with nrCMCs increased expression. After co-culture with nrCFBs, hESC-MSCs and fetal hMSCs did not express α-actinin and connexin43 expression was decreased. Conduction velocity (CV) in co-cultures of nrCMCs and hESC-MSCs was significantly higher than in co-cultures with fetal or adult hMSCs. In angiogenesis bioassays, only hESC-MSCs and fetal hMSCs were able to form capillary-like structures, which stained for smooth muscle and endothelial cell markers.Human embryonic and fetal MSCs differentiate toward three different cardiac lineages, in contrast to adult MSCs. Cardiomyogenesis is determined by stimuli from the cellular microenvironment, where connexin43 may play an important role.
Induction and Enhancement of Cardiac Cell Differentiation from Mouse and Human Induced Pluripotent Stem Cells with Cyclosporin-A
Masataka Fujiwara,Peishi Yan,Tomomi G. Otsuji,Genta Narazaki,Hideki Uosaki,Hiroyuki Fukushima,Koichiro Kuwahara,Masaki Harada,Hiroyuki Matsuda,Satoshi Matsuoka,Keisuke Okita,Kazutoshi Takahashi,Masato Nakagawa,Tadashi Ikeda,Ryuzo Sakata,Christine L. Mummery,Norio Nakatsuji,Shinya Yamanaka,Kazuwa Nakao,Jun K. Yamashita
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0016734
Abstract: Induced pluripotent stem cells (iPSCs) are novel stem cells derived from adult mouse and human tissues by reprogramming. Elucidation of mechanisms and exploration of efficient methods for their differentiation to functional cardiomyocytes are essential for developing cardiac cell models and future regenerative therapies. We previously established a novel mouse embryonic stem cell (ESC) and iPSC differentiation system in which cardiovascular cells can be systematically induced from Flk1+ common progenitor cells, and identified highly cardiogenic progenitors as Flk1+/CXCR4+/VE-cadherin? (FCV) cells. We have also reported that cyclosporin-A (CSA) drastically increases FCV progenitor and cardiomyocyte induction from mouse ESCs. Here, we combined these technologies and extended them to mouse and human iPSCs. Co-culture of purified mouse iPSC-derived Flk1+ cells with OP9 stroma cells induced cardiomyocyte differentiation whilst addition of CSA to Flk1+ cells dramatically increased both cardiomyocyte and FCV progenitor cell differentiation. Spontaneously beating colonies were obtained from human iPSCs by co-culture with END-2 visceral endoderm-like cells. Appearance of beating colonies from human iPSCs was increased approximately 4.3 times by addition of CSA at mesoderm stage. CSA-expanded human iPSC-derived cardiomyocytes showed various cardiac marker expressions, synchronized calcium transients, cardiomyocyte-like action potentials, pharmacological reactions, and ultra-structural features as cardiomyocytes. These results provide a technological basis to obtain functional cardiomyocytes from iPSCs.
The development of an internet-based outpatient cardiac rehabilitation intervention: a Delphi study
Corneel Vandelanotte, Trudy Dwyer, Anetta Van Itallie, Christine Hanley, W Kerry Mummery
BMC Cardiovascular Disorders , 2010, DOI: 10.1186/1471-2261-10-27
Abstract: A three-round Delphi study among cardiac rehabilitation experts was conducted. In the first round, 43 experts outlined opinions they had on the development of an online ORC platform into an open-ended electronic questionnaire. In the second round, 42 experts completed a structured (five-point scale) electronic questionnaire based on first round results, in which they scored items on their relevance. In the third round, the same experts were asked to re-rate the same items after feedback was given about the group median relevance score to establish a level of consensus.After the third round, high consensus was reached in 120 of 162 (74%) questionnaire items, of which 93 (57% of 162 items) also had high relevance according to the experts. The results indicate that experts strongly agreed on desired website content, data obtained from the patient, and level of interaction with patients that should be part of an Internet-based OCR intervention.The high rates of consensus and relevance observed among cardiac rehabilitation experts are an indication that they perceived the development and implementation of an Internet-based ORC intervention as feasible, and as a valuable alternative to face-to-face programs. In many ways the experts indicated that an Internet-based ORC program should mimic a traditional face-to-face program, and emphasize the crucial role of the cardiac rehabilitation manager who interacts with patients from a distance. The present study revealed practical insights into how Internet OCR interventions should be designed and opens the door for the development of such an intervention to be subsequently examined in a longitudinal and experimental study.In developed countries cardiovascular disease (CVD) continues to be the leading cause for mortality and morbidity in men and women and reducing its burden remains an important public health priority [1,2]. Controlling the CVD epidemic requires a multifaceted strategy targeting recognised modifiable risk factors
Activation of the Canonical Bone Morphogenetic Protein (BMP) Pathway during Lung Morphogenesis and Adult Lung Tissue Repair
Alexandros Sountoulidis, Athanasios Stavropoulos, Stavros Giaglis, Eirini Apostolou, Rui Monteiro, Susana M. Chuva de Sousa Lopes, Huaiyong Chen, Barry R. Stripp, Christine Mummery, Evangelos Andreakos, Paschalis Sideras
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041460
Abstract: Signaling by Bone Morphogenetic Proteins (BMP) has been implicated in early lung development, adult lung homeostasis and tissue-injury repair. However, the precise mechanism of action and the spatio-temporal pattern of BMP-signaling during these processes remains inadequately described. To address this, we have utilized a transgenic line harboring a BMP-responsive eGFP-reporter allele (BRE-eGFP) to construct the first detailed spatiotemporal map of canonical BMP-pathway activation during lung development, homeostasis and adult-lung injury repair. We demonstrate that during the pseudoglandular stage, when branching morphogenesis progresses in the developing lung, canonical BMP-pathway is active mainly in the vascular network and the sub-epithelial smooth muscle layer of the proximal airways. Activation of the BMP-pathway becomes evident in epithelial compartments only after embryonic day (E) 14.5 primarily in cells negative for epithelial-lineage markers, located in the proximal portion of the airway-tree, clusters adjacent to neuro-epithelial-bodies (NEBs) and in a substantial portion of alveolar epithelial cells. The pathway becomes activated in isolated E12.5 mesenchyme-free distal epithelial buds cultured in Matrigel suggesting that absence of reporter activity in these regions stems from a dynamic cross-talk between endoderm and mesenchyme. Epithelial cells with activated BMP-pathway are enriched in progenitors capable of forming colonies in three-dimensional Matrigel cultures. As lung morphogenesis approaches completion, eGFP-expression declines and in adult lung its expression is barely detectable. However, upon tissue-injury, either with naphthalene or bleomycin, the canonical BMP-pathways is re-activated, in bronchial or alveolar epithelial cells respectively, in a manner reminiscent to early lung development and in tissue areas where reparatory progenitor cells reside. Our studies illustrate the dynamic activation of canonical BMP-pathway during lung development and adult lung tissue-repair and highlight its involvement in two important processes, namely, the early development of the pulmonary vasculature and the management of epithelial progenitor pools both during lung development and repair of adult lung tissue-injury.
Qualitative and quantitative research into the development and feasibility of a video-tailored physical activity intervention
Corneel Vandelanotte, W Kerry Mummery
International Journal of Behavioral Nutrition and Physical Activity , 2011, DOI: 10.1186/1479-5868-8-70
Abstract: Five focus groups (n = 30), aimed at males and females, aged between 35 and 60 years, that do not meet the physical activity recommendation, were conducted to allow in-depth discussion of various elements related to the development of an online video-tailored intervention. In addition, a series of questions were delivered to a random sample (n = 1261) of Australians, using CATI survey technology, to gain more information and add a quantitative assessment of feasibility related to the development of the intervention. Focus group data was transcribed, and summarised using Nvivo software. Descriptive and frequency data of the survey was obtained using SPSS 18.0.Nearly all of the focus group participants supported the concept of a video-tailored intervention and 35.8% of survey participants indicated that they would prefer a video-based over a text-based intervention. Participants with a slow internet-connection displayed a lower preference for video-based advice (31.9%); however less than 20% of the survey sample indicated that downloading videos would be slow. The majority of focus group and survey participants did not support the idea of using mobile phones to receive this kind of intervention and indicated that video-tailored messages should be shorter than 5 minutes. Video-delivery of content is very rich in information, which increases the challenge to appropriately tailor content to participant characteristics; focus-group outcomes indicated a large diversity in participant preferences. 52.4% of survey participants indicated that the videos should be convincing and motivating.These results provide valuable information to develop an innovative video-tailored physical activity intervention. The results support the feasibility of such intervention, both in terms of users being ready to participate in it, as well as from a point of view whereby current internet infrastructure is able to cope with the demands of downloading videos. Though promising, a number of specif
Non-volant mammals of the Estación Biológica Allpahuayo: Assessment of the natural history and community ecology of a proposed reserve
Christine L. Hice
Mastozoolog?-a neotropical , 2004,
New technologies for people aging with a disability
Johanne L. Mattie,Christine Flegal
International Journal of Integrated Care , 2012,
A reconnaissance botanical survey of the Lower Orange River
H. Bezuidenhout,Christine L. Jardine
Koedoe : African Protected Area Conservation and Science , 2001, DOI: 10.4102/koedoe.v44i1.180
Abstract: In the past, the alluvial floodplains of the Orange River were covered with riverine shrubland and woodland. However, there are indications that this has changed and that this vegetation and habitat has been influenced by a number of factors, namely: expansion of irrigated crop farming; grazing pressure; and upstream dam construction. A botanical assessment was carried out along the Orange River between Blouputs and Onseepkans, in the Northern Cape to determine the nature and extent of the changes to the riverine vegetation, as well as to investigate the distribution of Combretum erythrophyllum and Rhus lancea. Only two specimens of Combretum erythrophyllum were noted whereas no Rhus lancea specimens were noted. There are indications that both the expansion of irrigated crop farming and grazing pressure has affected the vegetation of the plain and riverine area along the Orange River. Downstream from the farm Raap-en-Skraap (2819DA Skuitdrif), the natural vegetation of the riverbanks and islands is being replaced by alien plant species.
A course redesign project to change faculty orientation toward teaching
Susan Eliason,Christine L. Holmes
The Journal of Scholarship of Teaching and Learning , 2012,
Abstract: This article discusses the development, implementation, and outcomes of a Faculty Course Redesign Camp for full-time and adjunct faculty members. The purpose of the camp was to educate and coach faculty in effective strategies to promote learner-centered teaching skills. Evaluation results show that the participants changed their orientation toward teaching in the dimension of their role in instruction, but they made little change in balance of power and responsibility for learning.
ENDOGLIN Is Dispensable for Vasculogenesis, but Required for Vascular Endothelial Growth Factor-Induced Angiogenesis
Zhen Liu, Franck Lebrin, Janita A. Maring, Sander van den Driesche, Stieneke van der Brink, Maarten van Dinther, Midory Thorikay, Sabrina Martin, Kazuki Kobayashi, Lukas J. A. C. Hawinkels, Laurens A. van Meeteren, Evangelia Pardali, Jeroen Korving, Michelle Letarte, Helen M. Arthur, Charles Theuer, Marie-José Goumans, Christine Mummery, Peter ten Dijke
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0086273
Abstract: ENDOGLIN (ENG) is a co-receptor for transforming growth factor-β (TGF-β) family members that is highly expressed in endothelial cells and has a critical function in the development of the vascular system. Mutations in Eng are associated with the vascular disease known as hereditary hemorrhagic telangiectasia type l. Using mouse embryonic stem cells we observed that angiogenic factors, including vascular endothelial growth factor (VEGF), induce vasculogenesis in embryoid bodies even when Eng deficient cells or cells depleted of Eng using shRNA are used. However, ENG is required for the stem cell-derived endothelial cells to organize effectively into tubular structures. Consistent with this finding, fetal metatarsals isolated from E17.5 Eng heterozygous mouse embryos showed reduced VEGF-induced vascular network formation. Moreover, shRNA-mediated depletion and pharmacological inhibition of ENG in human umbilical vein cells mitigated VEGF-induced angiogenesis. In summary, we demonstrate that ENG is required for efficient VEGF-induced angiogenesis.
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