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Search Results: 1 - 10 of 80595 matches for " Chih-Kuan Chen "
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Universal protein fluctuations in populations of microorganisms
Hanna Salman,Naama Brenner,Chih-kuan Tung,Noa Elyahu,Elad Stolovicki,Lindsay Moore,Albert Libchaber,Erez Braun
Quantitative Biology , 2012,
Abstract: The copy number of any protein fluctuates among cells in a population; characterizing and understanding these fluctuations is a fundamental problem in biophysics. We show here that protein distributions measured under a broad range of biological realizations collapse to a single non-Gaussian curve under scaling by the first two moments. Moreover in all experiments the variance is found to depend quadratically on the mean, showing that a single degree of freedom determines the entire distribution. Our results imply that protein fluctuations do not reflect any specific molecular or cellular mechanism, and suggest that some buffering process masks these details and induces universality.
Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses
Tzi-Yuan Wang, Hsin-Liang Chen, Mei-Yeh J Lu, Yo-Chia Chen, Huang-Mo Sung, Chi-Tang Mao, Hsing-Yi Cho, Huei-Mien Ke, Teh-Yang Hwa, Sz-Kai Ruan, Kuo-Yen Hung, Chih-Kuan Chen, Jeng-Yi Li, Yueh-Chin Wu, Yu-Hsiang Chen, Shao-Pei Chou, Ya-Wen Tsai, Te-Chin Chu, Chun-Chieh A Shih, Wen-Hsiung Li, Ming-Che Shih
Biotechnology for Biofuels , 2011, DOI: 10.1186/1754-6834-4-24
Abstract: We have developed an efficient platform that uses a combination of transcriptomic and proteomic approaches to N. patriciarum to accelerate gene identification, enzyme classification and application in rice straw degradation. By conducting complementary studies of transcriptome (Roche 454 GS and Illumina GA IIx) and secretome (ESI-Trap LC-MS/MS), we identified 219 putative GH contigs and classified them into 25 GH families. The secretome analysis identified four major enzymes involved in rice straw degradation: β-glucosidase, endo-1,4-β-xylanase, xylanase B and Cel48A exoglucanase. From the sequences of assembled contigs, we cloned 19 putative cellulase genes, including the GH1, GH3, GH5, GH6, GH9, GH18, GH43 and GH48 gene families, which were highly expressed in N. patriciarum cultures grown on different feedstocks.These GH genes were expressed in Pichia pastoris and/or Saccharomyces cerevisiae for functional characterization. At least five novel cellulases displayed cellulytic activity for glucose production. One β-glucosidase (W5-16143) and one exocellulase (W5-CAT26) showed strong activities and could potentially be developed into commercial enzymes.Cellulosic ethanol produced by microbial fermentation from feedstocks has been proposed to replace fossil fuels in transportation. A key step in cellulosic ethanol production is to break down cellulose into glucose and hemicellulose into xylose, which can subsequently be converted into ethanol by fermentative microbes. Therefore, finding efficient cellulases is important to bioethanol production, as well as for hydrolyzing feedstocks into sugars in general. Neocallimastix species is one of the major anaerobic fungi in the rumen of water buffalo capable of efficiently digesting cellulosic biomass [1-4]. Such anaerobic fungi are potential sources for highly active cellulolytic enzymes that are useful for cellulose hydrolysis [5-7]. Plant cell wall degrading enzymes from rumen fungi such as Neocallimastix patriciarum may
論中國古代「藏書家」的定義:以明代為例 A Discussion on Definitions of Chinese Ancient Book Collectors in Ming Dynasty
Kuan-Chih Chen
Journal of Educational Media & Library Sciences , 2010,
Abstract: 藏書史研究的內容極為豐富,包括自古以來各種藏書機構和私人的藏書活動,以及典冊與圖書本身,還有諸如圖書的收集、購求、鑒別、保藏、研究與散佚等專業範疇,藏書思想與方法理論,也囊括與藏書活動關係密切的刻書、書肆、流通與傳播等社會風尚,和因藏書風尚產生的政治、經濟和社會文化背景等等,都是屬於中國文化史的一部份,非常值得深入探究。但是,藏書史研究往往受限於有關古代「藏書家」的定義,因為累朝歷代對於「典籍」的認知略有不同,才會造成藏書史研究上的根本問題。 反觀數十年來的藏書史研究,還沒有專書或專論針對古代藏書家的定義而作,事實上,在定義時也存有很大的困境。本文試圖從史料當中梳理出一條理路,以明代的藏書家為例,為中國古代藏書家的定義作出部份釐清與說明。 The book collection research contents are extremely rich, including each kinds of collection agencies and private collections since the ancient times, as well as book itself, and such as the book collection, purchase, identification, preservation, research, loss, thoughts and theoretical methods, and those customs are related closely to book collection activities about publishing, bookmen, circulation and dissemination, and the background of politics, economy, social culture that arise from the customs of book collection, they are all belong to the category of Chinese cultural history, and deserve to study deeply.However, the research of book-collecting history are always limited from the definition of ancient book collector, because of the recognition about the books in each period of time in ancient were different, and became a primary question of research in book-collecting history of ancient China. Surveying the research of the book-collecting, there are no any professional books or essays in definiens of Chinese book collectors.In fact, it’s hard to define it clearly. This article helps to realize the ancient book collectors clear by source books, discussing and explaining the definition of book collectors in Ming dynasty.
「中國現代圖書館運動皇后」──韋棣華女士對我國圖書館事業的貢獻 Mary Elizabeth Wood, the Queen of the Modern Library Movement in China, and Her Contribution to Chinese Library Establishment
陳冠至 Kuan-Chih Chen
Journal of Library and Information Science , 2012,
Abstract: 本文就現存文獻為基礎,闡述美國聖公會傳教士韋棣華女士對我國近現代圖書館事業啟蒙所作出的卓越貢獻及綜論她的歷史功績,給予適度評價。除藉以瞭解相關圖書館事業肇建歷程外,期盼透過強調韋氏的人格典範,重新喚起當時的「圖書館精神」,樹立為我輩當代與後世的楷模。頁次:87-93 This article gives an account, based on existing literature, of the outstanding contribution made by Mary Elizabeth Wood, who was a missionary of the American Episcopal Church, on the modern Chinese library establishment. It aims to offer an appropriate evaluation of her achievements. In addition to understanding the course of development for Chinese Librarianship, the exemplar of Wood’s personality is used to recall a former ‘library spirit’ lost, in hopes of reviving it as a model for the contemporary and generations to come. pp. 87-93
Successful High-Dosage Dexamethasone Treatment of H1N1 Influenza A Pneumonia Complicated with Acute Necrotizing Encephalitis in an HIV-Infected Adult  [PDF]
Kuan-Chih Chen, Chien-Hung Gow, Chia-Jui Yang, Hou-Tai Chang
Case Reports in Clinical Medicine (CRCM) , 2015, DOI: 10.4236/crcm.2015.47048
Abstract:
Neurological manifestations in H1N1 influenza A infection are very rare, especially in adults, and its mechanism of action is still uncertain. Here, we reported the case of a 53-year-old woman with human immunodeficiency virus infection (HIV) who had H1N1 influenza A pneumonia complicated with very rare acute necrotizing encephalitis, although the HIV was under control. With prompt identification and administration of high dosage of dexamethasone, her mental status improved from stupor to clear, with minimal right hemiparesis. Further, brain magnetic resonance image revealed great resolution of mass effect. This dramatic improvement in response to the treatment may improve our understanding of the pathophysiology between H1N1 influenza A infection and acute necrotizing encephalitis.
Optical-Electronic Properties of Carbon-Nanotubes Based Transparent Conducting Films  [PDF]
Kuan-Ru Chen, Hsiu-Feng Yeh, Hung-Chih Chen, Ta-Jo Liu, Shu-Jiuan Huang, Ping-Yao Wu, Carlos Tiu
Advances in Chemical Engineering and Science (ACES) , 2013, DOI: 10.4236/aces.2013.31013
Abstract:

Three coating methods (slot, dip and blade coatings) were used separately to coat a well-dispersed single-wall carbonnanotube (SWCNT) solution on polyethylene terephthalate (PET) film, and the resulting optical and electronic properties were measured and compared. It was found that the sheet resistance and the transparency of the SWCNT coated film decreased as the coating speed increased for dip and blade coatings, but were independent of the coating speed for slot coating. All three coating methods were able to produce transparent conductive film with transparency above 85% and sheet resistance close to 1000 ohm/sq. For industrial production, the slot die coating method appears to be more suitable in terms of high coating speed and uniformity of optical and electronic properties.

Optically Defined Modal Sensors Incorporating Spiropyran-Doped Liquid Crystals with Piezoelectric Sensors
Kuan-Ting Chen,Chin-Kai Chang,Hui-Lung Kuo,Chih-Kung Lee
Sensors , 2011, DOI: 10.3390/s110201810
Abstract: We integrated a piezoelectric sensing layer lamina containing liquid crystals (LC) and spiropyran (SP) in a LC/SP mixture to create an optically reconfigurable modal sensor for a cantilever beam. The impedance of this LC/SP lamina was decreased by UV irradiation which constituted the underlying mechanism to modulate the voltage externally applied to the piezoelectric actuating layer. Illuminating a specific pattern onto the LC/SP lamina provided us with a way to spatially modulate the piezoelectric vibration signal. We showed that if an UV illuminated pattern matches the strain distribution of a specific mode, a piezoelectric modal sensor can be created. Since UV illumination can be changed in situ in real-time, our results confirm for the first time since the inception of smart sensors, that an optically tailored modal sensor can be created. Some potential applications of this type of sensor include energy harvesting devices, bio-chips, vibration sensing and actuating devices.
SYMBOLIC AND FUNCTIONAL BRAND EFFECTS FOR MARKET SEGMENTATION
Kevin Kuan-Shun Chiu,Ru-Jen Lin,Maxwell K. Hsu,Shih-Chih Chen
Australian Journal of Business and Management Research , 2011,
Abstract: This study investigates the interrelationships among brand personality, brand preference, customer perceived value, and golfers’ performance in the context of Taiwan’s golf clubs market. The theoretical and statisticalrelationships among these constructs are developed and verified. Using survey data from 345 out of 1,000 randomly selected golfers, this study employs ANOVA, Factor Analysis, and Discriminant Analysis to examine the research hypotheses. The findings reveal that (1) notable brand personality factors including Reliability, Fashion, Masculine, Excitement, Wholesome, Leadership, Sentiment, Feminine, and Uniqueness could explain brand preference to a large extent, (2) golfers’ customer perceived value regarding various golf brands areconsiderably dissimilar, and (3) golfers’ deviation in performance significantly relates to brands. In addition to conventional bases for market segmentation (i.e., demographics, psychographics, lifestyles, etc.), this paper clearly provides practical guidelines of implementing brand personality for market segmentation and promotionstrategies. Brand personality proves to be a useful segmenting variable. The findings and the statistical results offer supportive evidence for implementing appropriate branding management on both functional (i.e., customer perceived value and performance) and emotional attributes (i.e., brand personality) in order to enhance competitiveness.
Combination of a Tension-Free Vaginal Tape Procedure and Laparoscopic-Assisted Vaginal Hysterectomy for the Treatment of Benign Uterine Disease Associated with Stress Urinary Incontinence
Hsi-Mi Liang,Kuan-Hui Huang,Fu-Tsai Kung,Chih-Wei Chen
Chang Gung Medical Journal , 2005,
Abstract: Background: Women undergoing hysterectomy for benign uterine disease (BUD) mayexperience stress urinary incontinence (SUI). We performed tension-freevaginal tape (TVT) procedure and laparoscopic-assisted vaginal hysterectomy(LAVH) simultaneously and assessed the feasibility and efficacy of TVTperformed under general anesthesia and the resultant anti-incontinenceeffects following the combined procedures.Methods: Between March, 2000 and March, 2002 inclusively, 63 patients, who sufferedfrom both BUD and SUI, underwent LAVH and TVT. Preoperativeevaluation included history-taking, physical examination and ultrasonography.One-hour pad test, multichannel urodynamics and urinary questionnairewere conducted preoperatively and postoperatively. Details about surgicalprocedures undertaken, hospitalization and urinary problems in the follow-upperiod were recorded.Results: 50 patients completed the study with a mean follow-up period of 34 (25-48)months. The mean age was 49 (39-67) years and mean parity 3 (2-6). Themean surgical duration was 163 (95-240) minutes and blood loss 284 (100-1,500) milliliters. Mean duration of hospital stay was 5.5 days and bladderdrainage 1.9 days. Three patients suffered bladder perforation and onepatient was complicated with excess blood loss. Postoperative urinary problemsincluded transient urine retention, de novo frequency/urgency symptomsand voiding difficultly.Conclusions: The efficacy of concomitant TVT in LAVH procedure remained satisfactoryin treatment of SUI associated with BUD. Both procedures reflect the benefitsof less-invasive surgery. So the combination of LAVH and TVT is probablya good alternative for the patient who needs to undergo hysterectomy andanti-incontinence surgery simultaneously.
A Comparison of Urodynamic Parameters and Lower Urinary Tract Symptoms in Urodynamic Genuine Stress Incontinence Women with or without Stress Urinary Incontinence.
Chih-Wei Chen,Kuan-Hui Huang,Hsi-Mi Liang,Hui-Tsen Hsu
Chang Gung Medical Journal , 2004,
Abstract: Background: The aim of this study was to investigate the urodynamic parameters andlower urinary tract symptoms (LUTS) of women with urodynamic genuinestress incontinence (GSI).Methods: A cross-sectional study involving 3323 women with LUTS was conducted.We recruited 1261 women with urodynamic GSI and were further grouped aswith or without symptomatic stress urinary incontinence (SUI). The LUTSand urodynamic measurements between the two groups were analyzed andcompared.Results: Women with symptomatic SUI had a greater maximal flow rate, larger voidedvolume, larger maximal cystometric capacity, and smaller maximal urethralclosure pressure (MUCP) during both rest and stress. The amount ofurine leakage during the 1-hour pad test of GSI women with symptomaticSUI was much greater than women without symptomatic SUI (26.0g vs 7.2g,p < 0.001). The significant differences in other lower urinary tract symptomsincluding nocturia, diurnal frequency, incomplete emptying, poor stream,urgency, urge incontinence, and post-void dribbling in women without symptomaticSUI were all less than women with symptomatic SUI.Conclusions: The urodynamic parameters and LUTS between the GSI women with orwithout symptomatic SUI are different. Approximate 15% of the GSI womendo not complain of symptomatic SUI. The lower urinary tract symptoms ofwomen with GSI without symptomatic SUI were less prominent than for theGSI women with symptomatic SUI.
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