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Search Results: 1 - 10 of 194489 matches for " Celso Oliveira de;Luiz "
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Comparison of DNA-extraction methods and Selective Enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR)
Freschi, Carla Roberta;Carvalho, Luiz Fernando de Oliveira e Silva;Oliveira, Celso José Bruno de;
Brazilian Journal of Microbiology , 2005, DOI: 10.1590/S1517-83822005000400011
Abstract: the aim of this study was to compare different dna-extraction methods and selective enrichment broths for their effectiveness to detect salmonella typhimurium in artificially inoculated swine feces samples (100 cfu/g) by polymerase chain reaction. after enrichment in rappaport-vassiliadis, selenite cystine or müller-kauffmann tetrathionate, aliquots were used for dna extraction by three different methods: boiling-centrifugation, phenol-chloroform and salting-out. aliquots of extracted dna were then used as template in pcr. the selective enrichment broths had no effect on the efficiency of pcr when boiling-centrifugation and salting-out were used. on the other hand, phenol-chloroform was superior (p<0.05) when combined to rappaport-vassiliadis. considering cost and efficiency parameters, we encourage the use of müller-kauffmann tetrathionate broth in combination with boiling-centrifugation dna-extraction procedure.
Effect of casting temperature and atmosphere on castability of Ni-Cr alloys: a comparative study with direct flame casting method
Bezzon, Osvaldo Luiz;Barros, Celso de;Pagnano, Valéria Oliveira;Soriani, Natércia Carreira;
Materials Research , 2006, DOI: 10.1590/S1516-14392006000200022
Abstract: the purpose of this study was to compare the effect of casting temperature and atmosphere on the castability of three ni-cr alloys against direct flame casting method. vera bond (vb), vera bond 2 (vb2) and wiron 99 (w99) were cast at three temperatures: vb and vb2 (1310 °c, 1340 °c and 1370 °c); w99 (1400 °c, 1430 °c and 1460 °c) in atmosphere, vacuum and direct flame. each alloy was cast in seven different conditions. castability was assessed by the method that verifies the alloy potential to reproduce a nylon mesh. kruskal-wallis test demonstrated for vb, while there was no influence in the temperature range, castability was greater by vacuum (99.3%) than by direct flame (96.2%) and atmosphere (93.06%). for vb2, castability was greater at evaluated temperatures (1370 °c = 94.4%, 1340 °c = 91.15% and 1310 °c = 87.9%) than direct flame (77.8%); related vacuum (97.33%) obtained better values than atmosphere (84.6%) and direct flame. for w99, while atmosphere had no influence, castability was higher at 1460 °c (84.3%) than at other temperatures (1430 °c = 70.3%, 1400 °c = 37.05%) and direct flame (62.5%). comparison among interactions showed that for all alloys it was possible to significantly increase the filling percentage of the mold in a comparative manner with the direct flame cast method.
O IBGE e as pesquisas populacionais
Oliveira, Luiz Antonio Pinto de;Sim?es, Celso Cardoso da Silva;
Revista Brasileira de Estudos de Popula??o , 2005, DOI: 10.1590/S0102-30982005000200007
Abstract: this article studies the history of the demographic censuses in brazil, as well as civil records and the pnad (national survey by household sampling), with emphasis on the main issues investigated by each of these instruments. the important role played by the state, especially in the development of the demographic censuses, is taken up, as this factor explains their rapid development. in contrast, during their early stages civil records remained dependent on the not always stable relationships between church and state. the increasing importance of the demographic censuses as instruments of analysis of demographic factors is gone into. the 1970 census is considered a milestone with respect to its organization, the wealth of details treated, and the reliability of the results. the role of civil society is also highlighted, as well as of the academic community and the formulators of public policies in this process. the pnad has also played a special role in this process, as it has been carried out between censuses, with multiple investigative purposes, sometimes including demographic characteristics (migration, fertility, marital unions, etc.) and factors related to health.
Efeitos do processamento da alfafa e da adi??o de óleo de soja sobre a digestibilidade total da dieta de eqüinos
Gobesso, Alexandre Augusto de Oliveira;Lorenzo, Celso Luiz Fernandes;Prezotto, Ligia Dias;Rennó, Francisco Palma;
Revista Brasileira de Zootecnia , 2009, DOI: 10.1590/S1516-35982009000400018
Abstract: the effect of alfalfa hay processing (medicago sativa l.) and the addition of soybean oil in diets on the total dry matter digestibility, organic matter, crude protein, ether extract, neutral detergent fiber and acid detergent in horses was evaluated. four crossbred male foals with approximately 14 months of age and 197.25 kg were used and assigned in a (4 × 4) latin square design with a factorial arrangement (2 × 2) composed of two alfalfa haying forms (cubes or branches) and the addition or absence of soybean oil in commercial diets containing concentrated in the form of pellets. the nutrients digestibility was determined by the total feces collection method for three days. the addition of refined soybean oil increased the total digestibility of dry matter, organic matter, ether extract and neutral detergent fiber. the alfalfa processing in the form of cubes increased the total digestibility of crude protein, neutral detergent fiber and acid detergent fiber. the addition of soybean oil in the assessed diets was a practical and safe way to increase the caloric density without reducing the digestibility of organic nutrients. the alfalfa processing in the form of cubes improved the total digestibility of crude protein, detergent fiber and acid detergent fiber in the diet of horses.
Angiossarcoma de Mama: Relato de Caso
Viviani Renata Silva de Oliveira,Gebrim Luiz Henrique,Nazário Afonso Celso Pinto,Kemp Cláudio
Revista Brasileira de Ginecologia e Obstetrícia , 2000,
Abstract: O angiossarcoma primário da mama é um tumor raro, que incide entre os 14 e 82 anos, com média aos 35 anos de idade. Seu aspecto clínico predominante é o de uma massa indolor, com aumento difuso na mama, que se apresenta com cor violácea ou enegrecida. Como ocorre com outros tipos de sarcoma, o tamanho médio da les o é de aproximadamente 5 cm quando do diagnóstico. Histologicamente, o angiossarcoma caracteriza-se pela prolifera o de células endoteliais que formam canais vasculares comunicantes entre si infiltrando estruturas glandulares e o tecido adiposo. Seu diagnóstico histológico é difícil e nem sempre é estabelecido de imediato, principalmente nos casos com baixo grau de malignidade, devido geralmente à escassez do material biopsiado. Pela dificuldade diagnóstica e pela agressividade, trata-se de neoplasia de prognóstico desfavorável pelas freqüentes metástases. Em nosso servi o, uma paciente de 18 anos procurou atendimento por apresentar nódulo doloroso de rápido crescimento, que foi biopsiado com diagnóstico de hemangioma, sendo indicada ressec o ampla. Três meses após, evoluiu com recidiva tumoral, que foi novamente biopsiada sendo indicada mastectomia, por tratar-se de angiossarcoma de baixo grau de malignidade. Após novas recidivas, indicou-se quimioterapia e, posteriormente, radioterapia. Em vigência desta, evoluiu com novas metástases, indo a óbito por metástase pulmonar.
Carlos SANDER,Fábio Luiz WANKLER,Renato Augusto de Oliveira EVANGELISTA,Celso Henrique MORAGA
Revista ACTA Geográfica , 2012,
Abstract: This article aims to discuss the flooding caused by the Branco river that affect the urban area of the city of Boa Vista. The Boa Vista city is installed on the right bank of the Branco river and is responsible for draining an area of 100,000 km2. The methodology involved a survey of the maximum annual flood of the Branco river in Boa Vista, the application of the Gumbel method to define there currence of flood event sand determining the area inundated by the worst flooding of the Branco river from the association's quota flood with the plantplanialtimetric of Boa Vista city.The results showed that the largest event of flooding of the Branco river occurred in June 2011, reaching the altimetry of 66.42m, covering an area of 6.16km2 (616 ha), but with a recurrence period estimated at 42 years. The second major event of the series occurred in 1976, reaching the altimetry of 65.95m, when affected an area of 5.85km2 and an expected turn around time of 21 years. The study points to the lack of data for defining full secular and the possible influences of engineering works at the level of major floods.
Efeitos da sinvastatina na hipertrofia lipídica da medula óssea induzida pelo uso de corticóides em ratos
Oliveira, Liszt Palmeira de;Penteado, Luiz Otávio Sampaio;Carvalho, Jorge José de;Mesquita, Karlos Celso de;
Revista Brasileira de Ortopedia , 2007, DOI: 10.1590/S0102-36162007001100005
Abstract: objectives: to evaluate the effects of using simvastatin (sv) on the medullary area occupied by adipocytes (maoa) in the femoral head of rats with lipidic hypertrophy induced by hydrocortisone (hc). methods: 40 male wistar rats (rattus norvegicus albinos) were distributed into five groups, each group with eight animals, according to the medication given daily during 15 days: hc (25 mg), hc (25 mg) + sb, hc (1 mg), hc (1 mg) + sv, and saline solution (sf). serum triglyceride (tri), total cholesterol (col), aspartate aminotransferase (ast), and alanine aminotransferase (alt) tests were performed before and after treatment. femoral head maoa was determined by morphometry. results: the groups had similar results of serum values of tri, col, ast, and alt in the beginning of the experiment. the animals that received hc had greater maoa when compared to those receiving sf (p < 0.0001), with statistically significant increases in the serum tri and col readings. maoa was greater in the groups receiving hc than in the groups receiving jc + sv (p < 0.0001). in the group that received hc (1 mg) + sv, there was no significant difference in maoa, when compared to the group that received sf (p = 0.5047), nor in the serum values of tri (p = 0.1907) and col (p = 0.4480), after the treatment. in the animals that received hc (25 mg) + sv, there was a significant difference in maoa, when compared to the group that received sf (p < 0.0001), and in the serum readings of tri (p = 0.0044) and col (p = 0.0025), after the treatment. all groups presented increased serum levels of ast and alt. the use of hc was related to a larger area occupied by adipocytes in the medulla of the femoral head, which was directly proportional to the dose given. this effect was reduced with the use of simvastatin. conclusion: the use of simvastatin to reduce the bone medulla lipidic content induced by hc was significant, and more consistent in the group treated with low hc doses.
Effect of non-surgical periodontal treatment on chronic kidney disease patients
Artese, Hilana Paula Carillo;Sousa, Celso Oliveira de;Luiz, Ronir Raggio;Sansone, Carmelo;Torres, Maria Cynésia Medeiros de Barros;
Brazilian Oral Research , 2010, DOI: 10.1590/S1806-83242010000400013
Abstract: chronic kidney disease (ckd) is a debilitating systemic condition. our working hypothesis is that ckd predialysis patients with periodontitis would respond poorly to periodontal treatment owing to immunologic compromise. twenty-one predialysis patients (group 1) and 19 individuals without clinical evidence of kidney disease (group 2) with chronic periodontitis were subjected to non-surgical periodontal treatment with no antibiotics. clinical periodontal and systemic parameters were evaluated at baseline and 3 months after treatment. both groups showed significant and similar post-treatment improvements in all periodontal parameters examined. most interestingly, periodontal treatment had a statistically significant positive effect on the glomerular filtration rate of each individual (group 1, p = 0.04; group 2, p = 0.002). our results indicate that chronic periodontitis in predialysis kidney disease patients improved similarly in patients with chronic periodontitis and no history of ckd after receiving non-surgical periodontal therapy. this study demonstrates that ckd predialysis patients show a good response to non-surgical periodontal treatment.
Comparative study of intermaxillary relationships of manual and swallowing methods
Alvarez, Márcia Cristina;Turbino, Miriam Lacale;Barros, Celso de;Pagnano, Valéria Oliveira;Bezzon, Osvaldo Luiz;
Brazilian Dental Journal , 2009, DOI: 10.1590/S0103-64402009000100014
Abstract: this study compared the mandibular displacement from three methods of centric relation record using an anterior jig associated with (a) chin point guidance, (b) swallowing (control group) and (c) bimanual manipulation. ten patients aged 25-39 years were selected if they met the following inclusion criteria: complete dentition (up to the second molars), angle class i and absence of signs and symptoms of temporomandibular disorders and diagnostic casts showing stability in the maximum intercuspation (mi) position. impressions of maxillary and mandibular arches were made with an irreversible hydrocolloid impression material. master casts of each patient were obtained, mounted on a microscope table in mi as a reference position and 5 records of each method were made per patient. the mandibular casts were then repositioned with records interposed and new measurements were obtained. the difference between the two readings allowed measuring the displacement of the mandible in the anteroposterior and lateral axes. data were analyzed statistically by anova and tukey's test at 5% significance level. there was no statistically significant differences (p>0.05) among the three methods for measuring lateral displacement (a=0.38 ± 0.26, b=0.32 ± 0.25 and c=0.32 ± 0.23). for the anteroposterior displacement (a=2.76 ± 1.43, b=2.46 ± 1.48 and c=2.97 ± 1.51), the swallowing method (b) differed significantly from the others (p<0.05), but no significant difference (p>0.05) was found between chin point guidance (a) and bimanual manipulation (c). in conclusion, the swallowing method produced smaller mandibular posterior displacement than the other methods.
Effect of plaque accumulation and salivary factors on enamel demineralization and plaque composition in situ
Tenuta, Livia Maria Andaló;Lima, José Eduardo de Oliveira;Cardoso, Celso Luiz;Tabchoury, Cínthia Pereira Machado;Cury, Jaime Aparecido;
Pesquisa Odontológica Brasileira , 2003, DOI: 10.1590/S1517-74912003000400006
Abstract: this study evaluated the effect of some plaque and salivary factors on caries progression in situ. the salivary secretion rate, buffering capacity and mutans streptococci counts from 13 volunteers were determined. for three distinct periods of time, 4, 7 and 10 days, each of them wore a palatal appliance containing 4 bovine enamel blocks. they used a non-fluoridated dentifrice during the experiment and a 20% sucrose solution was dripped onto the blocks 10 times a day. mutans streptococci (ms), calcium (ca), and insoluble polysaccharide (ip) were quantified in the dental plaque formed on the enamel blocks, after each period. enamel demineralization was assessed by surface microhardness, and the percentage of surface microhardness change (%smc) in relation to the baseline values was calculated. enamel demineralization occurred after each period of plaque accumulation (p < 0.05), and the %smc increased with time (from 13.8 to 48.3%). the concentrations of ca and ip in plaque were not statistically different among the experimental times, but significant correlations were found between these concentrations and %smc. neither the salivary factors assessed initially nor mutans streptococci in plaque presented statistically significant correlations to %smc. the results suggest that enamel demineralization is time-dependent and is more related to the composition of the biofilm formed than to the salivary factors studied.
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