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Search Results: 1 - 10 of 5071 matches for " Cecilia Prata "
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Dietary Phenolic Acids Act as Effective Antioxidants in Membrane Models and in Cultured Cells, Exhibiting Proapoptotic Effects in Leukaemia Cells
Laura Zambonin,Cristiana Caliceti,Francesco Vieceli Dalla Sega,Diana Fiorentini,Silvana Hrelia,Laura Landi,Cecilia Prata
Oxidative Medicine and Cellular Longevity , 2012, DOI: 10.1155/2012/839298
Abstract: Caffeic, syringic, and protocatechuic acids are phenolic acids derived directly from food intake or come from the gut metabolism of polyphenols. In this study, the antioxidant activity of these compounds was at first evaluated in membrane models, where caffeic acid behaved as a very effective chain-breaking antioxidant, whereas syringic and protocatechuic acids were only retardants of lipid peroxidation. However, all three compounds acted as good scavengers of reactive species in cultured cells subjected to exogenous oxidative stress produced by low level of H2O2. Many tumour cells are characterised by increased ROS levels compared with their noncancerous counterparts. Therefore, we investigated whether phenolic acids, at low concentrations, comparable to those present in human plasma, were able to decrease basal reactive species. Results show that phenolic acids reduced ROS in a leukaemia cell line (HEL), whereas no effect was observed in normal cells, such as HUVEC. The compounds exhibited no toxicity to normal cells while they decreased proliferation in leukaemia cells, inducing apoptosis. In the debate on optimal ROS-manipulating strategies in cancer therapy, our work in leukaemia cells supports the antioxidant ROS-depleting approach.
Effect of Plasma Membrane Cholesterol Depletion on Glucose Transport Regulation in Leukemia Cells
Cristiana Caliceti, Laura Zambonin, Cecilia Prata, Francesco Vieceli Dalla Sega, Gabriele Hakim, Silvana Hrelia, Diana Fiorentini
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0041246
Abstract: GLUT1 is the predominant glucose transporter in leukemia cells, and the modulation of glucose transport activity by cytokines, oncogenes or metabolic stresses is essential for their survival and proliferation. However, the molecular mechanisms allowing to control GLUT1 trafficking and degradation are still under debate. In this study we investigated whether plasma membrane cholesterol depletion plays a role in glucose transport activity in M07e cells, a human megakaryocytic leukemia line. To this purpose, the effect of cholesterol depletion by methyl-β-cyclodextrin (MBCD) on both GLUT1 activity and trafficking was compared to that of the cytokine Stem Cell Factor (SCF). Results show that, like SCF, MBCD led to an increased glucose transport rate and caused a subcellular redistribution of GLUT1, recruiting intracellular transporter molecules to the plasma membrane. Due to the role of caveolae/lipid rafts in GLUT1 stimulation in response to many stimuli, we have also investigated the GLUT1 distribution along the fractions obtained after non ionic detergent treatment and density gradient centrifugation, which was only slightly changed upon MBCD treatment. The data suggest that MBCD exerts its action via a cholesterol-dependent mechanism that ultimately results in augmented GLUT1 translocation. Moreover, cholesterol depletion triggers GLUT1 translocation without the involvement of c-kit signalling pathway, in fact MBCD effect does not involve Akt and PLCγ phosphorylation. These data, together with the observation that the combined MBCD/SCF cell treatment caused an additive effect on glucose uptake, suggest that the action of SCF and MBCD may proceed through two distinct mechanisms, the former following a signalling pathway, and the latter possibly involving a novel cholesterol dependent mechanism.
Phytochemical Profile and Nutraceutical Value of Old and Modern Common Wheat Cultivars
Emanuela Leoncini, Cecilia Prata, Marco Malaguti, Ilaria Marotti, Antonio Segura-Carretero, Pietro Catizone, Giovanni Dinelli, Silvana Hrelia
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0045997
Abstract: Among health-promoting phytochemicals in whole grains, phenolic compounds have gained attention as they have strong antioxidant properties and can protect against many degenerative diseases. Aim of this study was to profile grain phenolic extracts of one modern and five old common wheat (Triticum aestivum L.) varieties and to evaluate their potential antiproliferative or cytoprotective effect in different cell culture systems. Wheat extracts were characterized in terms of antioxidant activity and phenolic composition (HPLC/ESI-TOF-MS profile, polyphenol and flavonoid contents). Results showed that antioxidant activity (FRAP and DPPH) is mostly influenced by flavonoid (both bound and free) content and by the ratio flavonoids/polyphenols. Using a leukemic cell line, HL60, and primary cultures of neonatal rat cardiomyocytes, the potential antiproliferative or cytoprotective effects of different wheat genotypes were evaluated in terms of intracellular reactive oxygen species levels and cell viability. All tested wheat phenolic extracts exerted dose-dependent cytoprotective and antiproliferative effects on cardiomyocytes and HL60 cells, respectively. Due to the peculiar phenolic pattern of each wheat variety, a significant genotype effect was highlighted. On the whole, the most relevant scavenging effect was found for the old variety Verna. No significant differences in terms of anti-proliferative activities among wheat genotypes was observed. Results reported in this study evidenced a correspondence between the in vitro antioxidant activity and potential healthy properties of different extracts. This suggests that an increased intake of wheat grain derived products could represent an effective strategy to achieve both chemoprevention and protection against oxidative stress related diseases.
Steviol Glycosides Modulate Glucose Transport in Different Cell Types
Benedetta Rizzo,Laura Zambonin,Cristina Angeloni,Emanuela Leoncini,Francesco Vieceli Dalla Sega,Cecilia Prata,Diana Fiorentini,Silvana Hrelia
Oxidative Medicine and Cellular Longevity , 2013, DOI: 10.1155/2013/348169
Abstract: Extracts from Stevia rebaudiana Bertoni, a plant native to Central and South America, have been used as a sweetener since ancient times. Currently, Stevia extracts are largely used as a noncaloric high-potency biosweetener alternative to sugar, due to the growing incidence of type 2 diabetes mellitus, obesity, and metabolic disorders worldwide. Despite the large number of studies on Stevia and steviol glycosides in vivo, little is reported concerning the cellular and molecular mechanisms underpinning the beneficial effects on human health. The effect of four commercial Stevia extracts on glucose transport activity was evaluated in HL-60 human leukaemia and in SH-SY5Y human neuroblastoma cells. The extracts were able to enhance glucose uptake in both cellular lines, as efficiently as insulin. Our data suggest that steviol glycosides could act by modulating GLUT translocation through the PI3K/Akt pathway since treatments with both insulin and Stevia extracts increased the phosphorylation of PI3K and Akt. Furthermore, Stevia extracts were able to revert the effect of the reduction of glucose uptake caused by methylglyoxal, an inhibitor of the insulin receptor/PI3K/Akt pathway. These results corroborate the hypothesis that Stevia extracts could mimic insulin effects modulating PI3K/Akt pathway. 1. Introduction Stevia rebaudiana Bertoni is a weak perennial shrub belonging to Asteraceae (Compositae) family, native to subtropical regions of Brazil and Paraguay. Its leaves have been used as a sweetener since ancient times and for many other medicinal purposes in Latin America and the Orient for centuries [1, 2]. The “sweet herb” has gained increasing interest from nutritional researchers and commercial area in the last years, due to the growing need to find new natural calorie-free sweeteners alternative to sugar. Indeed, in both industrialized and developing countries, the incidence of type 2 diabetes mellitus and obesity is sharply increasing as a result of dietary behaviours, reduced physical activities, and ageing. These metabolic disorders have become major public health problems worldwide [3, 4]. Glycemic control is fundamental to the management of diabetes since it is associated with significantly decreased rates of retinopathy, nephropathy, neuropathy, and cardiovascular disease, the most common cause of death in diabetic patients. The effort to achieve near-normoglycemia through the key strategy of glycemic control includes recommendations for prevention and control of diabetes, for example, monitoring carbohydrate intake and limiting the consumption
Failure to launch?: The women?s movement struggle for abortion decriminalization in the Portuguese Parliament (1982)
Ex aequo , 2012,
Abstract: in 1982, the communist party presented a proposed law on abortion decriminalization and the women’s movement tactics converged at parliamentary members. despite that, we know little about this contested political process and the attempts for legal change that occurred from the interaction between activists, double-militants, and mps. findings show that women’s organizations remained as ‘outsiders’ and excluded from the political process, but that most of their preferred framings were put forward by their few political allies. the ?failure? to pass abortion reform in 1982 cannot overshadow what the debate ultimately represented - a precursor of the approval of abortion decriminalization in parliament just two years later, in 1984.
Cuando la "puesta en el juego" es un hijo que aún no ha nacido.
Giuliana Prata
Revista de la Asociación Espa?ola de Neuropsiquiatría , 1990,
Abstract: Los juegos de la pareja pueden influir en sus "puestas" un hijo que no ha nacido o que ni siquiera ha sido concebido. Síntomas tales como esterilidad, abortos espontáneos o muertes del ni o durante el parto son examinados en este artículo a la luz de esta hipótesis.
La maniobra del miembro ausente en las primeras sesiones de consulta. Cómo evitar errores irreparables.
Giuliana Prata
Revista de la Asociación Espa?ola de Neuropsiquiatría , 1985,
Abstract: Este artículo trata del problema que se presenta a los terapeutas cuando una familia citada a sesión durante la fase de evaluación aparece sin uno de sus miembros. La respuesta no varía: no se hace la sesión. Sin embargo, el terapeuta trata de obtener, de los miembros presentes, la mayor calidad de información posible a efecto de comprender el tipo de relación existente en esa familia en particular. Los otros elementos del encuadre terapéutico, horario, honorarios, etc., se manejan de acuerdo a aquello que pueda ser más efectivo y tener más impacto en el sistema. No se vuelve a dar una cita, se deja a la familia la iniciativa. Es un mensaje absolutamente claro en cuanto que es la familia la que debe decidir si va a venir y cuándo quiere hacerlo. Pero, si decide hacerlo, debe aceptar las indicaciones del terapeuta en el sentido de qué personas deben concurrir a la sesión. Esta contramaniobra se ha mostrado, en los casos que se detallan, absolutamente eficaz.
Del juego simétrico de la pareja al juego psicótico de la familia.
Giuliana Prata
Revista de la Asociación Espa?ola de Neuropsiquiatría , 1988,
Abstract: En este artículo, el autor, a través del informe de una historia familiar, hace hincapié en ciertas modalidades de interacción que se encuentran frecuentemente en familias con transacción psicótica. El confrontamiento entre la experiencia clínica y la observación informal de una familia ayuda a comprender mejor la evolución posible del 'juego simétrico" de la pareja hacía el 'juego psicótico" de la familia
The role of the scientific research in the control of schistosomiasis in endemic areas
Prata, Aluízio;
Memórias do Instituto Oswaldo Cruz , 2004, DOI: 10.1590/S0074-02762004000900002
Abstract: the way the researches established the lines of direction for considering fight against schistosomiasis on the double aspect of transmission and morbidity control is outstanding. chemotherapy in the morbidity control is emphasized. the research priorities for schistosomiasis control are mentioned.
Evolution of the clinical and epidemiological knowledge about Chagas disease 90 years after its discovery
Prata, Aluízio;
Memórias do Instituto Oswaldo Cruz , 1999, DOI: 10.1590/S0074-02761999000700008
Abstract: three different periods may be considered in the evolution of knowledge about the clinical and epidemiological aspects of chagas disease since its discovery: (a) early period concerning the studies carried out by carlos chagas in lassance with the collaboration of other investigators of the manguinhos school. at that time the disease was described and the parasite, transmitters and reservoirs were studied. the coexistence of endemic goiter in the same region generated some confusion about the clinical forms of the disease; (b) second period involving uncertainty and the description of isolated cases, which lasted until the 1940 decade. many acute cases were described during this period and the disease was recognized in many latin american countries. particularly important were the studies of the argentine mission of regional pathology studies, which culminated with the description of the roma?a sign in the 1930 decade, facilitating the diagnosis of the early phase of the disease. however, the chronic phase, which was the most important, continued to be difficult to recognize; (c) period of consolidation of knowledge and recognition of the importance of chagas disease. studies conducted by laranja, dias and nóbrega in bambuí updated the description of chagas heart disease made by carlos chagas and eurico villela. from then on, the disease was more easily recognized, especially with the emphasis on the use of a serologic diagnosis; (d) period of enlargement of knowledges on the disease. the studies on denervation conducted in ribeir?o preto by fritz k?berle starting in the 1950 decade led to a better understanding of the relations between chagas disease and megaesophagus and other visceral megas detected in endemic areas.
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