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Search Results: 1 - 10 of 405327 matches for " Cecilia Ahlstr?m Emanuelsson "
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A Randomized, Double-Blind, Placebo-Controlled Pilot Clinical Study on ColdZyme® Mouth Spray against Rhinovirus-Induced Common Cold  [PDF]
Mats Clarsund, Marcus Fornbacke, Lena Uller, Sebastian L. Johnston, Cecilia Ahlstrm Emanuelsson
Open Journal of Respiratory Diseases (OJRD) , 2017, DOI: 10.4236/ojrd.2017.74013
Abstract:
Common colds incur significant costs in terms of sick leave and personal discomfort for affected individuals. This study investigated the performance of ColdZyme Mouth Spray (ColdZyme), a protective barrier against common cold, in rhinovirus-inoculated healthy volunteers. This randomized, doubleblind, placebo-controlled pilot study was conducted on 46 healthy volunteers inoculated with rhinovirus 16 via the nose. Subjects self-administered ColdZyme or placebo 6 times daily for 11 days. Symptoms were recorded daily in a diary. Rhinovirus 16 in nasal and oropharyngeal samples at days 0, 3, 4, 6, 7 and 10 were quantified by RT-qPCR. The primary outcome measure was the reduction in viral load in oropharyngeal samples. Rhinovirus 16 was only detected in 35 out of 46 inoculated subjects. Exploratory analysis measuring the total viral load (i.e., area under the curve (AUC)) for days 3 - 10 in successfully inoculated subjects found that ColdZyme treatment resulted in a lower total viral load in the oropharynx (p = 0.023). In subjects who experienced symptomatic common cold, irrespectively, if virus were detected, treatment with ColdZyme resulted in a reduction in the number of days with common cold symptoms from 6.5 to 3.0 days (p = 0.014) in comparison to placebo. ColdZyme reduced virus infection in the oropharynx and reduced the number of days with common cold symptoms and highlights the possible importance of the oropharynx in common cold infections. Suitable outcome measures for a feasible study on ColdZyme are total viral load in the oropharynx in subjects having detectable virus present in nasal or oropharyngeal samples.
Effects of a dual CCR3 and H1-antagonist on symptoms and eosinophilic inflammation in allergic rhinitis
Lennart Greiff, Cecilia Ahlstrm-Emanuelsson, Ash Bahl, Thomas Bengtsson, Kerstin Dahlstr?m, Jonas Erjef?lt, Henrik Widegren, Morgan Andersson
Respiratory Research , 2010, DOI: 10.1186/1465-9921-11-17
Abstract: To examine whether a dual CCR3 and H1-receptor antagonist (AZD3778) affects allergic inflammation and symptoms in allergic rhinitis.Patients with seasonal allergic rhinitis were subjected to three seven days' allergen challenge series. Treatment with AZD3778 was given in a placebo and antihistamine-controlled design. Symptoms and nasal peak inspiratory flow (PIF) were monitored in the morning, ten minutes post challenge, and in the evening. Nasal lavages were carried out at the end of each challenge series and α2-macroglobulin, ECP, and tryptase were monitored as indices of allergic inflammation.Plasma levels of AZD3778 were stable throughout the treatment series. AZD3778 and the antihistamine (loratadine) reduced rhinitis symptoms recorded ten minutes post challenge during this period. AZD3778, but not the anti-histamine, also improved nasal PIF ten minutes post challenge. Furthermore, scores for morning and evening nasal symptoms from the last five days of the allergen challenge series showed statistically significant reductions for AZD3778, but not for loratadine. ECP was reduced by AZD3778, but not by loratadine.AZD3778 exerts anti-eosinophil and symptom-reducing effects in allergic rhinitis and part of this effect can likely be attributed to CCR3-antagonism. The present data are of interest with regard to the potential use of AZD3778 in allergic rhinitis and to the relative importance of eosinophil actions to the symptomatology of allergic rhinitis.EudraCT No: 2005-002805-21.The CC-chemokine receptor-3 (CCR3) is a transmembrane protein that constitutes one of the receptors for CC-chemokines. It is localized to cells of key importance to allergic inflammation including dendritic cells, Th2-lymphocytes, eosinophils, basophils, and mast cells as well as to epithelial, smooth muscle, and neural cells [1-7]. Chemokines interacting with CCR3 include eotaxin-1, 2, and 3, MCP-4, and RANTES [8]. Stimulation of the receptor produces chemotaxis and cellular activation [8]
Repeated intranasal TLR7 stimulation reduces allergen responsiveness in allergic rhinitis
Lennart Greiff, Anders Cervin, Cecilia Ahlstrm-Emanuelsson, Gun Almqvist, Morgan Andersson, Jan Dolata, Leif Eriksson, Edward D H?gest?tt, Anders K?llen, Per Norlén, Inga-Lisa Sj?lin, Henrik Widegren
Respiratory Research , 2012, DOI: 10.1186/1465-9921-13-53
Abstract: To evaluate the efficacy and safety of intranasal AZD8848.In a placebo-controlled single ascending dose study, AZD8848 (0.3-600?μg) was given intranasally to 48 healthy subjects and 12 patients with allergic rhinitis (NCT00688779). In a placebo-controlled repeat challenge/treatment study, AZD8848 (30 and 60?μg) was given once weekly for five weeks to 74 patients with allergic rhinitis out of season: starting 24 hours after the final dose, daily allergen challenges were given for seven days (NCT00770003). Safety, tolerability, pharmacokinetics, and biomarkers were monitored. During the allergen challenge series, nasal symptoms and lavage fluid levels of tryptase and α2-macroglobulin, reflecting mast cell activity and plasma exudation, were monitored.AZD8848 produced reversible blood lymphocyte reductions and dose-dependent flu-like symptoms: 30–100?μg produced consistent yet tolerable effects. Plasma interleukin-1 receptor antagonist was elevated after administration of AZD8848, reflecting interferon production secondary to TLR7 stimulation. At repeat challenge/treatment, AZD8848 reduced nasal symptoms recorded ten minutes after allergen challenge up to eight days after the final dose. Tryptase and α2-macroglobulin were also reduced by AZD8848.Repeated intranasal stimulation of Toll-like receptor 7 by AZD8848 was safe and produced a sustained reduction in the responsiveness to allergen in allergic rhinitis.NCT00688779 and NCT00770003 as indicated above.
Detection of Crosslinks within and between Proteins by LC-MALDI-TOFTOF and the Software FINDX to Reduce the MSMS-Data to Acquire for Validation
Christopher A. G. S?derberg, Wietske Lambert, Sven Kjellstr?m, Alena Wiegandt, Ragna Peterson Wulff, Cecilia M?nsson, Gudrun Rutsdottir, Cecilia Emanuelsson
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0038927
Abstract: Lysine-specific chemical crosslinking in combination with mass spectrometry is emerging as a tool for the structural characterization of protein complexes and protein-protein interactions. After tryptic digestion of crosslinked proteins there are thousands of peptides amenable to MSMS, of which only very few are crosslinked peptides of interest. Here we describe how the advantage offered by off-line LC-MALDI-TOF/TOF mass spectrometry is exploited in a two-step workflow to focus the MSMS-acquisition on crosslinks mainly. In a first step, MS-data are acquired and all the peak list files from the LC-separated fractions are merged by the FINDX software and screened for presence of crosslinks which are recognized as isotope-labeled doublet peaks. Information on the isotope doublet peak mass and intensity can be used as search constraints to reduce the number of false positives that match randomly to the observed peak masses. Based on the MS-data a precursor ion inclusion list is generated and used in a second step, where a restricted number of MSMS-spectra are acquired for crosslink validation. The decoupling of MS and MSMS and the peptide sorting with FINDX based on MS-data has the advantage that MSMS can be restricted to and focused on crosslinks of Type 2, which are of highest biological interest but often lowest in abundance. The LC-MALDI TOF/TOF workflow here described is applicable to protein multisubunit complexes and using 14N/15N mixed isotope strategy for the detection of inter-protein crosslinks within protein oligomers.
Vad erbjuds f r kunskap i l rarutbildningen och vad till gnar de studerande sig?
Karl-Georg Ahlstrm
Utbildning & Demokrati : Tidsskrift f?r Didaktik och Utbildningspolitik , 1999,
Abstract:
The identification of allergen proteins in sugar beet (Beta vulgaris) pollen causing occupational allergy in greenhouses
Susanne Luoto, Wietske Lambert, Anna Blomqvist, Cecilia Emanuelsson
Clinical and Molecular Allergy , 2008, DOI: 10.1186/1476-7961-6-7
Abstract: Sera from individuals at a local sugar beet seed producing company, having positive SPT and specific IgE to sugar beet pollen extract, were used for immunoblotting. Proteins in sugar beet pollen extracts were separated by 1- and 2-dimensional electrophoresis, and IgE-reactive proteins analyzed by liquid chromatography tandem mass spectrometry.A 14 kDa protein was identified as an allergen, since IgE-binding was inhibited by the well-characterized allergen Che a 2, profilin, from the related species Chenopodium album. The presence of 17 kDa and 14 kDa protein homologues to both the allergens Che a 1 and Che a 2 were detected in an extract from sugar beet pollen, and partial amino acid sequences were determined, using inclusion lists for tandem mass spectrometry based on homologous sequences.Two occupational allergens were identified in sugar beet pollen showing sequence similarity with Chenopodium allergens. Sequence data were obtained by mass spectrometry (70 and 25%, respectively for Beta v 1 and Beta v 2), and can be used for cloning and recombinant expression of the allergens. As for treatment of Chenopodium pollinosis, immunotherapy with sugar beet pollen extracts may be feasible.The prevalence of allergy is increasing and the causative agents are usually airborne environmental allergens [1], from furry animals (cat, dog etc) and small arthropods (dustmite, cockroach etc) and pollen from grasses, weeds and trees. The pollen type dominating as allergen source varying with the geographical region [2,3]. Occupational allergy constitutes a special problem, since intensive exposure to allergenic sources can result from specialised work processes. Examples are allergenic latex proteins to which health workers may become sensitized via latex-containing disposable gloves, or mouse urinary proteins for animal house attendants.In this study exposure to pollen in greenhouses is addressed. Sugar beet seed is produced in fields as well as in greenhouses. Attending the plants
Brief communication "The aerophotogrammetric map of Greenland ice masses"
M. Citterio,A. P. Ahlstrm
The Cryosphere Discussions , 2012, DOI: 10.5194/tcd-6-3891-2012
Abstract: The PROMICE (Programme for Monitoring of the Greenland Ice Sheet) aerophotogrammetric map of Greenland ice masses is the first high resolution dataset documenting the mid-1980's extent of the Greenland Ice Sheet and all the local glaciers and ice caps. The total glacierized area was 1 804 638 km2 ± 2178 km2, of which 88 083 ± 1240 km2 belonged to local glaciers and ice caps (GIC) substantially independent from the Greenland Ice Sheet. This new result of GIC glacierized area is higher than most previous estimates, and is in line with contemporary findings based on independent data sources. Comparison between our map and the recently released GIMP (Greenland Mapping Project) Ice Cover Mask (Howat and Negrete, 2012) show potential for change assessment studies.
Formation of anthropogenic secondary organic aerosol (SOA) and its influence on biogenic SOA properties
E. U. Emanuelsson,M. Hallquist,K. Kristensen,M. Glasius
Atmospheric Chemistry and Physics (ACP) & Discussions (ACPD) , 2013, DOI: 10.5194/acp-13-2837-2013
Abstract: Secondary organic aerosol (SOA) formation from mixed anthropogenic and biogenic precursors has been studied exposing reaction mixtures to natural sunlight in the SAPHIR chamber in Jülich, Germany. In this study aromatic compounds served as examples of anthropogenic volatile organic compound (VOC) and a mixture of α-pinene and limonene as an example for biogenic VOC. Several experiments with exclusively aromatic precursors were performed to establish a relationship between yield and organic aerosol mass loading for the atmospheric relevant range of aerosol loads of 0.01 to 10 μg m 3. The yields (0.5 to 9%) were comparable to previous data and further used for the detailed evaluation of the mixed biogenic and anthropogenic experiments. For the mixed experiments a number of different oxidation schemes were addressed. The reactivity, the sequence of addition, and the amount of the precursors influenced the SOA properties. Monoterpene oxidation products, including carboxylic acids and dimer esters were identified in the aged aerosol at levels comparable to ambient air. OH radicals were measured by Laser Induced Fluorescence, which allowed for establishing relations of aerosol properties and composition to the experimental OH dose. Furthermore, the OH measurements in combination with the derived yields for aromatic SOA enabled application of a simplified model to calculate the chemical turnover of the aromatic precursor and corresponding anthropogenic contribution to the mixed aerosol. The estimated anthropogenic contributions were ranging from small (≈8%) up to significant fraction (>50%) providing a suitable range to study the effect of aerosol composition on the aerosol volatility (volume fraction remaining (VFR) at 343 K: 0.86–0.94). The aromatic aerosol had higher oxygen to carbon ratio O/C and was less volatile than the biogenic fraction. However, in order to produce significant amount of aromatic SOA the reaction mixtures needed a higher OH dose that also increased O/C and provided a less volatile aerosol. The SOA yields, O/C, and f44 (the mass fraction of CO2+ ions in the mass spectra which can be considered as a measure of carboxylic groups) in the mixed photo-chemical experiments could be described as linear combinations of the corresponding properties of the pure systems. For VFR there was in addition an enhancement effect, making the mixed aerosol significantly less volatile than what could be predicted from the pure systems. A strong positive correlation was found between changes in volatility and O/C with the exception during dark hours where th
Early phase resolution of mucosal eosinophilic inflammation in allergic rhinitis
Lena Uller, Cecilia Emanuelsson, Morgan Andersson, Jonas S Erjef?lt, Lennart Greiff, Carl G Persson
Respiratory Research , 2010, DOI: 10.1186/1465-9921-11-54
Abstract: To determine mucosal eosinophilia, apoptotic eosinophils, general cell apoptosis and cell proliferation, and expression of CCL5 and CCL11 (eotaxin) in human allergic airway tissues in vivo at resolution of established symptomatic eosinophilic inflammation.Twenty-one patients with intermittent (birch and/or grass) allergic rhinitis received daily nasal allergen challenges for two seven days' periods separated by more than two weeks washout. Five days into these "artificial pollen seasons", nasal treatment with budesonide was instituted and continued for six days in a double blinded, randomized, placebo-controlled, and crossover design. This report is a parallel group comparison of nasal biopsy histochemistry data obtained on the final day of the second treatment period.Treatments were instituted when clinical rhinitis symptoms had been established. Compared to placebo, budesonide reduced tissue eosinophilia, and subepithelial more than epithelial eosinophilia. Steroid treatment also attenuated tissue expression of CCL5, but CCL11 was not reduced. General tissue cell apoptosis and epithelial cell proliferation were reduced by budesonide. However, apoptotic eosinophils were not detected in any biopsies, irrespective of treatment.Inhibition of CCL5-dependent recruitment of cells to diseased airway tissue, and reduced cell proliferation, reduced general cell apoptosis, but not increased eosinophil apoptosis, are involved in early phase steroid-induced resolution of human allergic rhinitis.Airway tissue signs of established inflammation in asthma and allergic rhinitis include increased mucosal tissue cell turnover, eosinophilia, and increased chemokine production. Institution of steroid treatment eventually reduces both symptoms and eosinophilic inflammation in allergic airways diseases [1]. However, early resolution effects in vivo of this mainstay class of airway drugs are incompletely understood.In recent years, based largely on observations in vitro, the view that est
GCM characteristics explain the majority of uncertainty in projected 21st century terrestrial ecosystem carbon balance
A. Ahlstrm,J. Lindstr?m,M. Rummukainen,B. Smith
Biogeosciences Discussions , 2012, DOI: 10.5194/bgd-9-13685-2012
Abstract: One of the largest sources of uncertainties in modelling of the future global climate is the response of the terrestrial carbon cycle. Studies have shown that it is likely that the extant land sink of carbon will weaken in a warming climate. Should this happen, a~larger portion of the annual carbon dioxide emissions will remain in the atmosphere, and further increase the global warming, which in turn may further weaken the land sink. We investigate the potential sensitivity of global terrestrial ecosystem carbon balance to differences in future climate simulated by four general circulation models (GCMs) under three different CO2 concentration scenarios. We find that the response in simulated carbon balance is more influenced by GCMs than CO2 concentration scenarios. Singular Value Decomposition (SVD) analysis of sea surface temperatures (SSTs) reveals differences in the GCMs SST variability leading to decreased tropical ecosystem productivity in two out of four GCMs. We extract parameters describing GCM characteristics by parameterizing a statistical replacement model mimicking the simulated carbon balance results. By sampling two GCM-specific parameters and global temperatures we create 60 new "artificial" GCMs and investigate the extent to which the GCM characteristics may explain the uncertainty in global carbon balance under future radiative forcing. Our analysis suggests that differences among GCMs in the representation of SST variability and ENSO and its effect on precipitation and temperature patterns explains the majority of the uncertainty in the future evolution of global terrestrial ecosystem carbon.
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