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Search Results: 1 - 10 of 465553 matches for " Catherine A. Wardius "
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A generalised module for the selective extracellular accumulation of recombinant proteins
Yanina R Sevastsyanovich, Denisse L Leyton, Timothy J Wells, Catherine A Wardius, Karina Tveen-Jensen, Faye C Morris, Timothy J Knowles, Adam F Cunningham, Jeffrey A Cole, Ian R Henderson
Microbial Cell Factories , 2012, DOI: 10.1186/1475-2859-11-69
Abstract: Here, we report the development of a generalised module, based on an E. coli autotransporter secretion system, for the production of extracellular recombinant proteins. We demonstrate that a wide variety of structurally diverse proteins can be secreted as soluble proteins when linked to the autotransporter module. Yields were comparable to those achieved with other bacterial secretion systems.The advantage of this module is that it relies on a relatively simple and easily manipulated secretion system, exhibits no apparent limitation to the size of the secreted protein and can deliver proteins to the extracellular environment at levels of purity and yields sufficient for many biotechnological applications.
Mutational and Topological Analysis of the Escherichia coli BamA Protein
Douglas F. Browning, Sophie A. Matthews, Amanda E. Rossiter, Yanina R. Sevastsyanovich, Mark Jeeves, Jessica L. Mason, Timothy J. Wells, Catherine A. Wardius, Timothy J. Knowles, Adam F. Cunningham, Vassiliy N. Bavro, Michael Overduin, Ian R. Henderson
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0084512
Abstract: The multi-protein β-barrel assembly machine (BAM) of Escherichia coli is responsible for the folding and insertion of β-barrel containing integral outer membrane proteins (OMPs) into the bacterial outer membrane. An essential component of this complex is the BamA protein, which binds unfolded β-barrel precursors via the five polypeptide transport-associated (POTRA) domains in its N-terminus. The C-terminus of BamA contains a β-barrel domain, which tethers BamA to the outer membrane and is also thought to be involved in OMP insertion. Here we mutagenize BamA using linker scanning mutagenesis and demonstrate that all five POTRA domains are essential for BamA protein function in our experimental system. Furthermore, we generate a homology based model of the BamA β-barrel and test our model using insertion mutagenesis, deletion analysis and immunofluorescence to identify β-strands, periplasmic turns and extracellular loops. We show that the surface-exposed loops of the BamA β-barrel are essential.
Reduced Expression of Inflammatory Genes in Deceased Donor Kidneys Undergoing Pulsatile Pump Preservation
Valeria R. Mas, Kellie J. Archer, Catherine I. Dumur, Mariano J. Scian, Jihee L. Suh, Anne L. King, Megan E. Wardius, Julie A. Straub, Marc P. Posner, Kenneth Brayman, Daniel G. Maluf
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0035526
Abstract: Background The use of expanded criteria donor kidneys (ECD) had been associated with worse outcomes. Whole gene expression of pre-implantation allograft biopsies from deceased donor kidneys (DDKs) was evaluated to compare the effect of pulsatile pump preservation (PPP) vs. cold storage preservation (CSP) on standard and ECD kidneys. Methodology/Principal Findings 99 pre-implantation DDK biopsies were studied using gene expression with GeneChips. Kidneys transplant recipients were followed post transplantation for 35.8 months (range = 24–62). The PPP group included 60 biopsies (cold ischemia time (CIT) = 1,367+/?509 minutes) and the CSP group included 39 biopsies (CIT = 1,022+/?485 minutes) (P<0.001). Donor age (42.0±14.6 vs. 34.1±14.2 years, P = 0.009) and the percentage of ECD kidneys (PPP = 35% vs. CSP = 12.8%, P = 0.012) were significantly different between groups. A two-sample t-test was performed, and probe sets having a P<0.001 were considered significant. Probe set level linear models were fit using cold ischemia time and CSP/PPP as independent variables to determine significant probe sets (P<0.001) between groups after adjusting for cold ischemia time. Thus, 43 significant genes were identified (P<0.001). Over-expression of genes associated with inflammation (CD86, CD209, CLEC4, EGFR2, TFF3, among others) was observed in the CSP group. Cell-to-cell signaling and interaction, and antigen presentation were the most important pathways with genes significantly over-expressed in CSP kidneys. When the analysis was restricted to ECD kidneys, genes involved in inflammation were also differentially up-regulated in ECD kidneys undergoing CSP. However, graft survival at the end of the study was similar between groups (P = 0.2). Moreover, the incidence of delayed graft function was not significant between groups. Conclusions/Significance Inflammation was the most important up-regulated pattern associated with pre-implantation biopsies undergoing CSP even when the PPP group has a larger number of ECD kidneys. No significant difference was observed in delayed graft function incidence and graft function post-transplantation. These findings support the use of PPP in ECD donor kidneys.
Antimicrobial Resistance and Plasmid Profiles of Campylobacter Species from Infants Presenting with Diarrhoea in Osun State, Nigeria  [PDF]
Olutoyin Catherine Adekunle, Abiodun A. Onilude
Open Journal of Medical Microbiology (OJMM) , 2015, DOI: 10.4236/ojmm.2015.51003
Abstract: Antibiotic resistance among enteric bacterial pathogens complicates the heavy diarrhoea disease burden. Antimicrobial resistance of Campylobacter spp. to fluoroquinolones, which are generally used for the treatment of bacterial gastroenteritis, has increased during the past two decades, mainly as a result of the approval of this group of antimicrobials for use in food-producing animals. The aim is to determine the frequency of resistance of campylobacter to various antimicrobial agents and the relationship between antimicrobial agents of the isolates and the presence of plasmid. Twenty five Campylobacter isolates gotten from humans were subjected to antibiotics testing using Kirby Bauer disc diffusion method as well as standard E-test method. The plasmid profile of the isolates was determined using the Alkaline phosphatise procedure. The antimicrobial susceptibility testing of these isolates showed that all were sensitive to Erythromycin and Ciprofloxacin while none was sensitive to co-trimoxazole. The standard organisms were sensitive to co-trimoxazole (80%) and ciprofloxacin (65%) but were resistant to erythromycin (70%). No plasmid was found in streptomycin and ampicillin resistant strains, with the exception of four isolates which were co-trimoxazole-resistant and which contained around 24.4kb plasmids.
Incidence of Gonorrhea and Chlamydia in Urban Settings: The Case for Neighborhood Level Analysis in Boston  [PDF]
Catherine Cattley, Paola Massari, Caroline A. Genco
Advances in Infectious Diseases (AID) , 2015, DOI: 10.4236/aid.2015.54020
Abstract: The sexually transmitted infections (STIs) gonorrhea and chlamydia are known to disproportionately affect impoverished communities and communities of color, especially in urban areas. Moreover, socioeconomic and demographic factors such as poverty and race/ethnicity may contribute to a difference in treatment setting choice as well as a delay in care seeking. In an urban metropolitan area such as Boston, the overall gonorrhea and chlamydia rates are higher than national rates, and such differences are even more marked in certain neighborhoods with greater proportions of individuals who are impoverished, young, and of color. Using a retrospective analysis of city wide data, we highlight the effects of socioeconomic and demographic variables on urban STI prevalence. High poverty rates, race/ethnicity and younger adult populations are linked to disproportionately high STI rates. Interestingly, STI rates do not appear to be influenced by the universal health care coverage offered to the whole Massachusetts’ resident populations. We examine the effects of these variables in Boston neighborhoods in conjunction to STI rates and hypothesize that the observed rates are underestimates of the true prevalence of infection. Future studies will investigate how these same socioeconomic and demographic factors influence which treatment settings are chosen and subsequently lead to a delay in treatment.
Medication Use by Runners in Self-Care Situations  [PDF]
David A. Taylor, Catherine D. Santanello
Pharmacology & Pharmacy (PP) , 2017, DOI: 10.4236/pp.2017.88019
Abstract: The benefits of running for cardiovascular health have long been established, but no relationship between runners/non-runners and their usage of prescription and over-the-counter (OTC) medications has been established. A comprehensive survey was sent out via Facebook, Inc., to self-identified runners to assess runners’ first response to 5 common self-care situations. The results were compared to the national average of the U.S. population who take prescription and OTC medications. What was also assessed was the relationship between average weekly miles run and the likelihood to choose OTC medications. 714 runners, residing predominately in the St. Louis, Missouri metropolitan area, completed the survey and their results were drastically different than the national average use for prescription and OTC medication in the general (non-runners) population. Approximately 30% of the runners in this study are on daily prescription medications versus a national average of 70% of the U.S. population. In each of the 5 common self-care situations, less than 50% of runners chose an OTC medication as their first option for self-care vs. the national average of 80%. Results of the study also showed that runners with a weekly mile average of 30 miles or more were less likely to choose an OTC option for self-care than runners with a weekly average of 15 miles or less. Overall, results of this survey suggest that runners are less likely to take medications and may be healthier than the average U.S. citizen overall.
11th Intelligent Systems for Molecular Biology 2003 (ISMB 2003)
Catherine A. Abbott
Comparative and Functional Genomics , 2003, DOI: 10.1002/cfg.336
Abstract: This report profiles the keynote talks given at ISMB03 in Brisbane, Australia by Ron Shamir, David Haussler, John Mattick, Yoshihide Hayashizaki, Sydney Brenner, the Overton Prize winner, Jim Kent, and the ISCB Senior Accomplishment Awardee, David Sankov.
Génétique formelle des pigmentations humaines à variations continues: beaucoup d'hypothèses, peu de conclusions
Catherine Bonaiti, A Langaney
Genetics Selection Evolution , 1981, DOI: 10.1186/1297-9686-13-1-37
High quality sperm for nonhuman primate ART: Production and assessment
Catherine A VandeVoort
Reproductive Biology and Endocrinology , 2004, DOI: 10.1186/1477-7827-2-33
Abstract: The necessity of high quality sperm preparations may appear less important as ART advance in nonhuman primates. However, the study of intracytoplasmic sperm injection (ICSI) in humans and nonhuman primates has revealed that the use of randomly selected sperm for this procedure can result in various anomalies of sperm decondensation and embryonic development [1]. In a review of human clinical ICSI reports, chromosomal and genetic abnormalities are increased, but are most likely a result of underlying parental risk of the couples that require this procedure to achieve pregnancy [2]. These studies have underscored the importance of sperm quality for ART procedures.The processing of rhesus monkey (Macaca mulatta) semen for recovery of high quality spermatozoa has its basis in methods developed more than 20 years ago when in vitro fertilization (IVF) was first achieved for rhesus monkey oocytes [3]. The medium used for washing spermatozoa from the seminal plasma as a modification of Tyrode's medium supplemented with bovine serum albumin (BSA) that was originally developed by Bavister [4] for use in hamster IVF. This medium was improved with the addition of lactate and pyruvate, known as TALP, by Bavister and Yanigamachi [5] also for use in hamster IVF experiments.Macaque spermatozoa do not spontaneously capacitate and acquire the ability to bind to the zona pellucida as do human and some other species of spermatozoa. It was critical to the development of methods for macaque IVF that sperm be capacitated and capable of completing fertilization. Therefore, the next important modification in macaque spermatozoa processing was the discovery that cAMP and caffeine were required for rhesus monkey sperm to acquire fertilizing ability [6]. This chemical method for capacitation is often referred to as "activation."The development of the TALP medium and the addition of the cAMP and caffeine set the stage for successful IVF of macaque oocytes. The first successful IVF in the rhesus
Teachers Building Dwelling Thinking with Slideware
Catherine A Adams
Indo-Pacific Journal of Phenomenology , 2010,
Abstract: Teacher-student discourse is increasingly mediated through, by and with information and communication technologies: in-class discussions have found new, textually-rich venues online; chalk and whiteboard lectures are rapidly giving way to PowerPoint presentations. Yet, what does this mean experientially for teachers? This paper reports on a phenomenological study investigating teachers’ lived experiences of PowerPoint in post-secondary classrooms. As teachers become more informed about the affordances of information and communication technology like PowerPoint and consequently take up and use these tools in their classrooms, their teaching practices, relations with students, and ways of interpreting the world are simultaneously in-formed – conformed, deformed and reformed – by the given technology-in-use. The paper is framed in light of Martin Heidegger’s “Building Dwelling Thinking” (1951) and “The Thing” (1949). In these writings, Heidegger shows how a thing opens a new world to us, revealing novel structures of experience and meaning, and inviting us to a different style of being, thinking and doing. Indo-Pacific Journal of Phenomenology, May 2010, Volume 10, Edition 1
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