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Search Results: 1 - 10 of 559832 matches for " Carolina B. A. Restini "
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The Effect of Extracellular pH Changes on Intracellular pH and Nitric Oxide Concentration in Endothelial and Smooth Muscle Cells from Rat Aorta
Verena K. Capellini, Carolina B. A. Restini, Lusiane M. Bendhack, Paulo R. B. Evora, Andréa C. Celotto
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0062887
Abstract: Aims It has been known for more than a century that pH changes can alter vascular tone. However, there is no consensus about the effects of pH changes on vascular response. In this study, we investigated the effects of extracellular pH (pHo) changes on intracellular pH (pHi) and intracellular nitric oxide concentration ([NO]i) in freshly isolated endothelial cells and cross sections from rat aorta. Main Methods The HCl was used to reduce the pHo from 7.4 to 7.0 and from 7.4 to 6.5; the NaOH was used to increase the pHo from 7.4 to 8.0 and from 7.4 to 8.5. The fluorescent dyes 5-(and-6)-carboxy SNARF-1, acetoxymethyl ester, acetate (SNARF-1) and diaminofluorescein-FM diacetate (DAF-FM DA) were employed to measure the pHi and [NO]i, respectively. The fluorescence intensity was measured in freshly isolated endothelial cells by flow cytometry and in freshly obtained aorta cross sections by confocal microscopy. Key Findings The endothelial and vascular smooth muscle pHi was increased at pHo 8.5. The extracellular acidification did not change the endothelial pHi, but the smooth muscle pHi was reduced at pHo 7.0. At pHo 8.5 and pHo 6.5, the endothelial [NO]i was increased. Both extracellular alkalinization and acidification increased the vascular smooth muscle [NO]i. Significance Not all changes in pHo did result in pHi changes, but disruption of acid-base balance in both directions induced NO synthesis in the endothelium and/or vascular smooth muscle.
Vascular Relaxation Induced by C-Type Natriuretic Peptide Involves the Ca2+/NO-Synthase/NO Pathway
Fernanda A. Andrade, Carolina B. A. Restini, Marcella D. Grando, Leandra N. Z. Ramalho, Lusiane M. Bendhack
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0095446
Abstract: Aims C-type natriuretic peptide (CNP) and nitric oxide (NO) are endothelium-derived factors that play important roles in the regulation of vascular tone and arterial blood pressure. We hypothesized that NO produced by the endothelial NO-synthase (NOS-3) contributes to the relaxation induced by CNP in isolated rat aorta via activation of endothelial NPR-C receptor. Therefore, the aim of this study was to investigate the putative contribution of NO through NPR-C activation in the CNP induced relaxation in isolated conductance artery. Main Methods Concentration-effect curves for CNP were constructed in aortic rings isolated from rats. Confocal microscopy was used to analyze the cytosolic calcium mobilization induced by CNP. The phosphorylation of the residue Ser1177 of NOS was analyzed by Western blot and the expression and localization of NPR-C receptors was analyzed by immunohistochemistry. Key Findings CNP was less potent in inducing relaxation in denuded endothelium aortic rings than in intact ones. L-NAME attenuated the potency of CNP and similar results were obtained in the presence of hydroxocobalamin, an intracellular NO0 scavenger. CNP did not change the phosphorylation of Ser1177, the activation site of NOS-3, when compared with control. The addition of CNP produced an increase in [Ca2+]c in endothelial cells and a decrease in [Ca2+]c in vascular smooth muscle cells. The NPR-C-receptors are expressed in endothelial and adventitial rat aortas. Significance These results suggest that CNP-induced relaxation in intact aorta isolated from rats involves NO production due to [Ca2+]c increase in endothelial cells possibly through NPR-C activation expressed in these cells. The present study provides a breakthrough in the understanding of the close relationship between the vascular actions of nitric oxide and CNP.
Resveratrol Reverses the Impaired Vasodilation Observed in 2K-1C Hypertension through Endothelial Function Improvement  [PDF]
B. F. M. Pereira, A. C. Scalabrini, T. S. Marinho, C. R. K. Antonietto, C. B. A. Restini
Open Journal of Medicinal Chemistry (OJMC) , 2014, DOI: 10.4236/ojmc.2014.42004
Abstract:
Background: The production of endothelial-derived factors induces either vasoconstriction or vasodilation; nitric oxide (NO) is the most distinguished relaxing factor. Endothelial dysfunction is associated with hypertension. The partial loss in the NO-promoted vasodilation is due to its decreased bioavailability and/or to an activity reduction of endothelium NO synthase (eNOS). Reactive oxygen species (ROS), present in oxidative stress, seize NO and diminish its bioavailability. Transresveratrol (RESV) has been proved to increase NO and eNOS levels. Thus, RESV could be capable of improving NO dependent vascular relaxation on aortic rings isolated from treated 2K-1C animals through ROS damage reduction. Aim: Evaluate the effects of RESV treatment on the relaxation of aortic rings isolated from treated 2K-1C rats while focusing on the effects of the treatment on systolic blood pressure. Methods: Male Wistar rats (180 g) were grouped: two 2K-1C and two Sham groups, one of each was treated with RESV (20 mg/kg, gavage) dissolved in Tween 80 and one of each was treated with water plus Tween 80 (control) for six weeks. The rats had their systolic blood pressure (SBP) measured before and after the treatments. Vascular reactivity studies were conducted in order to observe and compare acetylcholine (ACh)-induced relaxations in the presence and absence of the NOS inhibitor L-NAME (10-4 mol/L). Results: SBP for 2K-1C was significantly reduced in the treated group (179.13 ± 4.90 mmHg, n = 23) when compared to the untreated group (196.66 ± 6.06 mmHg, n = 15, p < 0.01). The maximum relaxation of aortic rings isolated from the 2K-1C treated group showed a higher efficacy (116.63% ± 1.72%, n = 12) than that from the untreated group (85.97% ± 0.69%, n = 6, p < 0.001); L-NAME exposure was responsible for a significant decrease in each group’s maximum relaxation efficacy. Conclusions: SBP reduction observed after RESV treatment in rat renal hypertension could be due to the reestablishment of vascular relaxation depend of NO.
Antioxidant Protective Effects of the Resveratrol on the Cardiac and Vascular Tissues from Renal Hypertensive Rats  [PDF]
Joyce Cristina de Oliveira, Carla Renata Kitanish Antonietto, Angélica Cristina Scalabrini, Talita Sanches Marinho, Larissa Pernomian, José Wilson N. Corrêa, Carolina Baraldi Araujo Restini
Open Journal of Medicinal Chemistry (OJMC) , 2012, DOI: 10.4236/ojmc.2012.23008
Abstract: Background: Accumulating reactive oxygen species (ROS) is involved in cellular signaling and function disturbances due to the oxidative stress, which contributes to several diseases. The consequences of ROS activity represent an im-portant mechanism on the pathogenesis of vascular diseases, such as hypertension. Increased blood pressure observed in renal hypertension of the 2 kidneys-1 clip (2K-1C) model involves increased ROS levels in the cardiovascular system. Resveratrol, a polyphenolic compound primarily found in red wine, has many biological and pharmacological proper-ties. Considering the antioxidant properties of resveratrol, the present study was aimed to investigate the effects of the chronic treatment with resveratrol on cardiovascular system from renal hypertensive rats. Results: 2K-1C hypertension presented increased blood pressure, which was reduced at the end of the fifth week of resveratrol treatment. The cardiac hypertrophy index and the basal levels of ROS in rat aortic rings were also reduced by resveratrol treatment. Conclusions: The present findings clearly show the protective effects from resveratrol on the blood pressure, the car-diac growth and the vascular ROS generation in renal hypertension.
Role of endothelium on the abnormal Angiotensin-mediated vascular functions in epileptic rats  [PDF]
Carolina Restini, Rosana Reis, Claudio Costa-Neto, Norberto Garcia-Cairasco, José Cortes-de-Oliveira, Lusiane Bendhack
Journal of Biophysical Chemistry (JBPC) , 2012, DOI: 10.4236/jbpc.2012.32019
Abstract: Epidemiological studies have found that the risk for cardiovascular disease is increased in patients with epilepsy. The Renin Angiontensin System (RAS), an important player in vascular tone control, is also involved in many neurological disorders, including seizures and epilepsy. Although it has been reported that Angiotensin II (Ang II) release and Angiotensin receptors expression are altered in many cerebral areas in patients/animal models with neurological disorders, there are no data on the vascular function. We evaluated Ang I and Ang II-mediated vascular responses and to correlate their contractile responses to the pres- ence of endothelium and the protein levels of components of the RAS (AT1, AT2, Mas and ACE) in aorta isolated from genetically epileptic rats (WAR strain). The major finding was that the vascular contractile response induced by Ang I and Ang II is endothelium-dependent. Ang II induced contractions in aortas from Wistar rats either with intact endothelium (E+) (1.16 ± 0.04 g, n = 6) and endothelium-denuded (E-) (1.24 ± 0.04 g, n = 6). Maximum contractile response (ME) induced by Ang I was lower in Wistar E+ (0.45 ± 0.03 g, n = 6) compared with Wistar E- (1.13 ± 0.08 g, n = 6). Ang I and Ang II failed to induce contraction in WAR E+, whereas the ME induced by Ang I in WAR E- was lower (0.52 ± 0.04 g, n = 11) than in the Wistar. ME induced by Ang II in aortas from WAR was also lower (0.40 ± 0.03 g, n = 11) compared with Wistar. AT1 receptor expression in both E+ WAR and Wistar was lower than in both E- WAR and Wistar. AT2 and Mas receptor expression was higher in Wistar E- and E+ as compared to WAR E- and E+. ACE expression was higher in both E+ WAR and Wistar, but it was lower in both E- WAR and Wistar. Endothelium impairs the contractile response induced by Angiotensin in WAR, suggesting that endothelial relaxing factors play important role on the aorta contraction.
Entre el control y la autonomía: políticas de salud sexual de jóvenes y mujeres en Colombia, 1964-1991
Morales B,María Carolina;
Avances en Enfermería , 2011,
Abstract: since the mid sixties sexual and reproductive health policies were formulated for youngsters and women for birth control, family planning and maternal-infant care. in the mid seventies, sexual education was established, abortion was penalized and core components of sexual and reproductive health currently known in colombia were defined. finally, in the 1990 decade, women's agency and students' mobilization gave way to a social and political change which meant a true preparation for the national constituent assembly (asamblea nacional constituyente) and for their recognition as political actors. the consequential conquest of political rights was an obstacle for sexual and reproductive rights debated in the country since 1994; they were not recognized by the colombian state. this article offers an analysis of the political components of these policies to explain the change from control to autonomy. it has two parts; in the first part, the beginning of the configuration of current sexual and reproductive policies in the country and in the second part, arising and recognition of social-political actors who promoted them: youngsters and women. the political component is highlighted as it is the core of the power movement and a determining factor in the configuration of said policies and concerns existing among youngsters and women, opposite to what has been said about these actors' lifestyle.
Paleogene flora of the Sloggett Formation, Tierra del Fuego, Argentina
Panti,Carolina; Marenssi,Sergio A; Olivero,Eduardo B;
Ameghiniana , 2008,
Abstract: in the southeastern corner of the isla grande of tierra del fuego, southernmost argentina, small outcrops representing late eocene-early oligocene sedimentation in fluvial environments, are referred to the sloggett formation. this unit crops out along the shores of bahía sloggett, where it is represented by carbonaceous mudstones, sandstones and conglomerates. a new paleofloristic collection of this site, originally reported by andersson at the beginning of the 20th, was collected and analyzed, revealing the presence of gymnosperm and angiosperm leaves. conifers are represented by probable podocarpaceae and the presence of araucariaceae is confirmed by leaves with preserved anatomy. angiosperms, although fragmentary, have been grouped in morphotypes, which are referred to the nothofagaceae, myrtaceae and lauraceae. the sloggett paleoflora is similar in composition to other contemporary floras, described from southern south america and that originated in temperate to cold-temperate and humid forest.
Diagnóstico genético en disquinesia ciliar primaria: Revisión bibliográfica
Grau L,Carolina; González B,Sergio; Fonseca A,Ximena;
Revista de otorrinolaringología y cirugía de cabeza y cuello , 2011, DOI: 10.4067/S0718-48162011000100011
Abstract: primary cilliary dyskinesia (pcd) is an heterogeneous genetic disease caused by a structural and/or functional alteration of the ciliary skeleton. it is a diagnostic challenge due to its protean clinical presentation and to the complexity of screening and diagnostic methods. the method hitherto regarded as the gold standard is the analysis of ciliary structure by transmission electron microscopy (tem). this presents limitations because analyzes a limited number of ciliary axonemes, and may exclude cases with typical functional and clinical presentation. in recent years new diagnostic methods have been developed based on novel knowledge of the structural ciliary proteins, the genes encoding these proteins and mutations associated to dcp. these new methods include genetic analysis and the study of protein expression in cilia of the affected patients. this paper reviews dcp pathophysiology, the current diagnostic methods applied, and summarizes the international literature regarding the diagnosis of dcp based on genetic screening.
Diagnóstico genético en disquinesia ciliar primaria: Revisión bibliográfica Genetic diagnosis in primary ciliary dyskinesia: Literature review
Carolina Grau L,Sergio González B,Ximena Fonseca A
Revista de Otorrinolaringología y Cirugía de Cabeza y Cuello , 2011,
Abstract: La disquinesia ciliar primaria (DCP) corresponde a una enfermedad genética heterogénea, que se produce por una alteración estructural o funcional de los cilios. Es de difícil diagnóstico tanto por su variada sintomatología como por la existencia de métodos de screening y diagnóstico complejos. El método que hasta ahora ha sido considerado como gold standard es el análisis de la estructura ciliar por medio de la microscopía electrónica de transmisión (MET). Esta técnica tiene limitaciones porque permite analizar un número limitado de axonemas ciliares y puede excluir del diagnóstico a pacientes con axonema normal pero con alteración funcional y clínica clásicas. En los últimos a os se han desarrollado métodos diagnósticos sobre la base de un mejor conocimiento de la estructura proteica de los cilios, de los genes que codifican estas proteínas y de las mutaciones asociadas a DCP. Estos nuevos métodos consisten en un análisis genético y un estudio de la expresión de proteínas ciliares en los pacientes afectados. Esta publicación tiene como objetivo realizar una revisión de la fisiopatología de la DCP, los métodos diagnósticos actuales y resumir el desarrollo del diagnóstico genético en la literatura internacional y su posible aplicación en nuestro medio. Primary cilliary dyskinesia (PCD) is an heterogeneous genetic disease caused by a structural and/or functional alteration of the ciliary skeleton. It is a diagnostic challenge due to its protean clinical presentation and to the complexity of screening and diagnostic methods. The method hitherto regarded as the gold standard is the analysis of ciliary structure by transmission electron microscopy (TEM). This presents limitations because analyzes a limited number of ciliary axonemes, and may exclude cases with typical functional and clinical presentation. In recent years new diagnostic methods have been developed based on novel knowledge of the structural ciliary proteins, the genes encoding these proteins and mutations associated to DCP. These new methods include genetic analysis and the study of protein expression in cilia of the affected patients. This paper reviews DCP pathophysiology, the current diagnostic methods applied, and summarizes the international literature regarding the diagnosis of DCP based on genetic screening.
Genetic diversity and population structure of Prochilodus costatus and Prochilodus argenteus preceding dam construction in the Paraopeba River, São Francisco River Basin, Minas Gerais, Brazil  [PDF]
Tatiana M. Barroca, Fábio P. Arantes, Bárbara F. Magalh?es, Flávia F. Siqueira, Carolina C. R. Horta, Isabella F. Pena, Jorge A. Dergam, Evanguedes Kalapothakis
Open Journal of Genetics (OJGen) , 2012, DOI: 10.4236/ojgen.2012.22017
Abstract: Curimat?-pioa (Prochilodus costatus) and curimat?-pacu (Prochilodus argenteus) are migratory fish species endemic to the S?o Francisco River Basin in Brazil. Both species play important roles in local fisheries and ecology in the Paraopeba River. A dam was recently constructed on this river and to help in the development and conservation programs, we characterized the genetic variation of both species before dam construction. Complex hypervariable repeats micro-satellite was used to asses genetic variation for both species within and between the five collection sites in order to detect population substructuring. Nucleotide substitutions and insertion/deletion polymorphisms (indels) resulted in 35 P. costatus haplotypes (sample size = 89) and 22 P. argenteus haplotypes (sample size = 32). Significant genetic diversity and population differentiation was detected between five sampling sites for both species. Therefore, each of the five sites should be regarded as a group comprising significant genetic differences in species conservation and maintenance plans. Comparing these results to genetic diversity measures after dam construction will be critical for future management in this region.
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