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Search Results: 1 - 10 of 12295 matches for " Carmen Australia Paredes Marcondes;Pachnicki "
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Estudo da correla??o do IMC e comprimento do intestino delgado em pacientes obesos submetidos à cirurgia bariátrica
Nassif, Paulo Afonso Nunes;Malafaia, Osvaldo;Ribas, Carmen Australia Paredes Marcondes;Pachnicki, Jan Pawel Andrade;Kume, Márcio Hiroaki;Macedo, Larissa Maria;Rikimaru, Tiele Assis;
ABCD. Arquivos Brasileiros de Cirurgia Digestiva (S?o Paulo) , 2009, DOI: 10.1590/S0102-67202009000300004
Abstract: background: according to the hypothesis of long bowel, the length of small intestine is related to obesity. there are evolutionary, anatomics, neuroendocrines evidences in favor of this assertion. aim: to check the measure of the small intestine length in overweight patients submitted to bariatric surgery and to analyze the correlation between the intestine size and body mass index (bmi). methods: the sample was composed of 30 patients of hospital universitário evangélico de curitiba, obesity service, which had been submitted to bariatric surgery in the period between march and june, 2009. these patients previously had been evaluated, according to their weight, height and bmi. the surgical technique was fobi-capella. the measurement of small bowel was carried through the treitz angle towards the ileocecal junction, during the operation. the instrument used was an intestinal manipulation clamp, which was marked with the measure of 10 cm. the intestinal handles had been measured in the anti-mesenteric edge, applying minimum tension necessary to rectify them. the data were statistically correlated and automatically tabulated, using the pearson correlation method. results: the average intestinal length for males was 582,5 cm and for 509,1 cm, medium length 528,7 cm. pearson correlation coefficient, confirmed the absence of correlation between bmi and the intestinal length. conclusion: the medium length of small intestine was 528,7 cm and it did not have correspondence to bmi.
Regenera??o do fígado de ratos após oclus?o parcial da drenagem venosa hepática
Aguiar, Luiz Roberto Farion de;Nassif, Paulo Afonso Nunes;Ribas, Carmen Australia Paredes Marcondes;Nascimento, Marcelo Mazza do;Wiederker, Julio Cesar;Pachnicki, Jan Pawel Andrade;Stieven-Filho, Edmar;
ABCD. Arquivos Brasileiros de Cirurgia Digestiva (S?o Paulo) , 2009, DOI: 10.1590/S0102-67202009000200005
Abstract: background: hepatic regeneration is a mechanism to overcome the loss of liver functional tissue. this process has been studied through different methods. aim: to evaluate the effect of the partial occlusion of the hepatic venous drainage on the regeneration of the remainder livers in rats submitted to partial hepatectomy.method: liver biopsies from 30 male wistar rats were collected, and after they were submitted two-third hepatectomy. the animals were divided in three groups: a control group and two study groups, which were submitted to different degrees of the right hepatic vein stenosis. after 96 hours of the stimulation for regeneration all rats were submitted to another hepatic biopsy. the analysis of the fragments was performed by immunohistochemistry for the ki-67 and von willebrand factor markers. the reading of the samples was done using the samba 4000 system. collagen deposition was evaluated by the trichromic masson's staining.result: the cellular proliferation of the animals submitted to partial hepatectomy and stenosis of the right hepatic vein persists higher when compared to control group. the label index for ki-67 was significantly higher post-hepatectomy in groups submitted to hepatic vein partial occlusion. the expression of von willebrand factor was strongly decreased after the hepatectomy in all groups. there was little deposit of collagen in the hepatic tissue of animals with hepatic vein stenosis. conclusion: partial occlusion of the hepatic venous drainage in rats submitted to partial hepatectomy prolongs the time of hepatic cells proliferation when compared to the animals with vein of normal caliber. consequently, there will be also a delay in the restoration of the extracellular matrix and in the formation of new sinusoidal vessels.
Correla o entre o doppler da veia hepática direita com a biópsia transcutanea guiada pela ultrassonografia em hepatopatias Correlation between doppler of the right hepatic vein with ultrasound transcutaneous guided biopsy in liver diseases
Gleim Dias de Souza,Luciana Rodrigues Queiroz,Carmen Australia Paredes Marcondes Ribas,Marcelo Mazza do Nascimento
Revista do Colégio Brasileiro de Cirurgi?es , 2012,
Abstract: OBJETIVOS: Correlacionar os achados da biópsia transcutanea hepática guiada por ultrassonografia com os dados ultrassonográficos modo B e Doppler da veia hepática direita; comparar os padr es de onda entre os grupos de estudo (hepatopatas) e controle (sadios); e avaliar se o Doppler da veia hepática direita serve como marcador de hepatopatia cr nica. MéTODOS: Foram estudados 38 pacientes portadores de hepatopatia cr nica comprovada por sorologia e biópsia (grupo de estudo) e dez pacientes sem hepatopatia sorológica (grupo controle), avaliados pela ultrassonografia modo B e Doppler. Os critérios histológicos foram a classifica o da Sociedade Brasileira de Patologia de Hepatite Cr nica. RESULTADOS: A ultrassonografia modo B e o Doppler diferenciaram os indivíduos portadores de hepatopatia cr nica dos normais (p=0,047). Houve diferen a significativa entre o grupo de estudo e o controle na compara o entre os achados histopatológicos, ultrassonográficos modo B e o Doppler nos padr es de onda da veia hepática direita (p=0,001). CONCLUS O: Foi possível correlacionar a biópsia hepática com a ultrassonografia modo B e o Doppler da veia hepática direita; os hepatopatas apresentaram altera o no fluxo da veia hepática direita e os normais n o, sendo que o padr o de onda nos controles saudáveis foi trifásico e nos hepatopatas bifásico ou monofásico; e o Doppler da veia hepática direita serviu como marcador de hepatopatia cr nica. OBJECTIVE: To correlate chronic liver disease diagnosed by transcutaneous liver biopsy guided by ultrasound, with ultrasound findings with B-mode and Doppler of the right hepatic vein; 2) to compare the wave patterns between the study group and the control group; 3) to compare the right hepatic vein Doppler findings with histopathology findings as a possible marker of chronic liver disease. METHODS: Were studied 38 patients with chronic liver disease diagnosed by biopsy and serology (study group) and 10 persons without serologic liver disease (control group), assessed only by ultrasound B-mode and Doppler. The criteria were based on histology classification of the Brazilian Society of Pathology for chronic hepatitis. Chi-square, Fisher's exact and Student t tests were used. RESULTS: The B-mode and Doppler ultrasound were useful in inferring the differentiation between individuals with chronic liver disease from normal. There were significant differences between the study group and the controls when comparing the histopathology findings, ultrasound B-mode and Doppler in relationship to the wave patterns of the right hepatic vein. CONCLUSI
Contagem de plaquetas e caracteriza??o clínica de úlceras de perna anticardiolipinas positivas
Skare, Thelma Laroka;Ribas, Carmen Autralia Paredes Marcondes;Malafaia, Osvaldo;Ribas Filho, Jurandir Marcondes;Nassif, Paulo Afonso Nunes;Nascimento, Marcelo Mazza do;Pachnicki, Jan Pawel Andrade;
Revista do Colégio Brasileiro de Cirurgi?es , 2009, DOI: 10.1590/S0100-69912009000500010
Abstract: objective: to study the prevalence of anticardiolipin antibodies in patients with venous, diabetic and arterial leg ulcers and to verify if platelet count, previous history of venous thrombosis, obstetrical history and the finding of livedo reticularis are markers of this autoantibody positivity. methods: 151 patients with leg ulcer (81 with venous, 50 with diabetic and 20 with arterial ulcers) and 150 controls were included. in both groups search for the presence of igg and igm anticardiolipin and platelet count was done. in the leg ulcer group demographic data, obstetrical history, previous history of venous thrombosis as well as presence of livedo reticularis by physical examination were pointed out. data was grouped in contingency and frequency tables and the tests of fisher and chi-squared were used for nominal variables and mann whitney and kruskall wallis for numerical variables. the adopted significance was of 5%. results: it was found an anticardiolipin prevalence of 7.2% (n=12) in the leg ulcer group and of 1.3% (n=2) in the control group (p=0.01). leg ulcer patients with anticardiolipin did not differ from those without it in gender (p=0.98), previous history of venous thrombosis (p=0.69), previous history of abortions (p=0.67) and platelet count (p=0.67). only two patients had livedo reticularis which precluded any conclusion on this data. conclusion: there is an increased prevalence of anticardiolipin antibodies in the general population with leg ulcers. clinical characteristics of ulcers as well as platelet count do not help in the identification of these patients.
Avalia??o da capilaroscopia usando Endotelina-1 como um marcador de ativa??o endotelial na les?o microvascular e úlceras cutaneas
Michaelis, Thiago;Andretta, Marianne;Albers, Carolina;Skare, Thelma Larocca;Ribas, Carmen Australia Paredes Marcondes;Moreira, Luciana Bugmann;
Revista do Colégio Brasileiro de Cirurgi?es , 2012, DOI: 10.1590/S0100-69912012000200008
Abstract: objectives: to evaluate the presence of et-1 in patients with scleroderma and its correlation with the level of disease activity; to verify if the levels of endothelin are associated with the clinical profile and autoantibodies of scleroderma, and even if there is an association with microvascular injury detected by nailfold capillaroscopy. methods: a total of 74 patients, 37 patients with scleroderma, the remaining being controls, were subjected to measurement of et-1 by elisa. patients with scleroderma were evaluated through a questionnaire about characteristics of the disease and determination of autoantibodies. disease severity was defined by the criteria of medsger and microvascular disease was accessed through nailfold capillaroscopy. results: of the 37 patients with scleroderma, three (8.1%) were men and 34 (91.89%) women, with a mean age of 48.97 ± 13.36 years and mean disease duration of 42.54 ± 13, 35. the amounts of et-1 in the controls was 0.41 to 5.65 pg / ml (median of 2.26 pg / ml) and, in the scleroderma group, from 0.41 to 8.82 pg / ml (median, 0.41 pg / ml), with p = 0.0007. there was no correlation with disease duration, patient age and the degree of skin involvement. no correlation was found between serum levels of et-1 and disease severity (p = 0.13). higher levels of et-1 were observed in the form of overlap (1.49 to 6.82 pg / ml). conclusion: the levels of et-1 in scleroderma were inferior to controls. there was no association of et-1 levels with the variables studied.
Significado clínico-patológico das express?es citofotométricas do Ki-67 e Caspase-3 no carcinoma de células escamosas do es?fago
Silva, Gilmar Pereira;Malafaia, Osvaldo;Cuenca, Ronaldo Máfia;Ribas-Filho, Jurandir Marcondes;Nassif, Paulo Afonso Nunes;Ribas, Carmen Australia Paredes Marcondes;Zeve, Jorge Luiz de Matos;
ABCD. Arquivos Brasileiros de Cirurgia Digestiva (S?o Paulo) , 2008, DOI: 10.1590/S0102-67202008000200008
Abstract: background: the esophageal squamous cell carcinoma treatment strategy is still based on the tumor staging, where tumor histopathologic charac-teristics are the major determinants. in parallel, studies have been developed in order to better understand the tumor biology using immunohistochemical meth-ods with manual quantification evaluating the proliferative and apoptotic activi-ties of the cells. the disadvantages related to the manual method rose the de-velopment of computerized ways to do the image analysis. objetives: to verify the expressions of the markers ki-67 (proliferative) and caspase-3 (apoptotic) and to correlate them with the clinic and pathologic characteristics of the tumor. methods: twenty-nine paraffin embedded blocks were studied, each one con-taining tissue samples from patients with esophageal squamous cell carcinoma submitted to esophagectomies. the clinic and pathological data were obtained from histopathologic informations and from medical records. the slides were prepared following the routine immunohistochemical method until the point to utilize the specific antibodies (mib-1 and cpp32). positive quantification of the immunoreactivity to the proteins ki-67 and caspase-3 was performed by the software for computerized image analysis samba (systeme d' analyse micro-photometrique a balayage automatique). statistical analysis was done having p<0.05 considered significant. results: there was predominance of males (82.7%); age over 50 years; moderately differentiated tumors (68.98%); tumor stage iii (72.42%); diameter of lesions >3cm; and lesions located in the lower third of the organ. the mean score indexes found were 62.05% for ki-67 and 86.06% for caspase-3 and there was no correlation with the clinic or pathologi-cal characteristics as gender, age and tumor staging. there was significant dif-ference of ki-67 expression among the histological grades (p=0.047) and corre-lation between the evaluated indexes (r=0.41 and p=0.032). conclusion: the p
Regenera??o do fígado após hepatectomia parcial em ratos submetidos à hipertens?o portal pós-hepática
Aguiar, Luiz Roberto Farion de;Nassif, Paulo Afonso Nunes;Ribas, Carmen Australia Paredes Marcondes;Czeczko, Nicolau Gregori;Ribas, Mauricio Marcondes;Marinho Júnior, Carlos Hespanha;Wendler, Eduardo;
ABCD. Arquivos Brasileiros de Cirurgia Digestiva (S?o Paulo) , 2011, DOI: 10.1590/S0102-67202011000200011
Abstract: background: the normal adult liver is quiescent and only a small percentage of its cells is subjected to cell division at any time, but can quickly initiate cell proliferation in response to a stimulus. this process can be triggered by partial hepatectomy. aim: to evaluate the effect of portal hypertension caused by partial occlusion of hepatic venous drainage on regeneration of remnant liver of rats after partial hepatectomy. methods: it was performed two-thirds hepatectomy in 50 adult male wistar rats. the animals were divided into five groups: a control group and four study groups were subjected to different degrees of plication of inferior vena cava-hepatic above. after 240 hours of the stimulus for regeneration took place relaparotomy with measurement of portal pressure and inferior vena cava, and liver biopsy. fragments were analyzed by immunohistochemistry for the markers ki-67 and von willebrand factor. the collagen deposition was evaluated by masson's trichrome staining and liver function using serological markers. results: cell proliferation in animals subjected to partial hepatectomy and portal hypertension persisted in varying degrees higher when compared to the control group. the proliferation index for ki-67 was higher in the groups submitted to the elevation of portal pressure. the expression of von willebrand factor was markedly elevated after partial hepatectomy in groups with higher degree of portal hypertension. there was little collagen deposition in liver tissue in animals of the four groups with partial plication of the inferior vena cava above-liver, but the deposition was more intense in the group with lower portal pressure. conclusions: rats underwent 70% partial hepatectomy and portal hypertension, after 240 hours, presented: 1. delay in the regenerative process directly proportional to pressure levels in the portal system; 2. after ten days, there was the proliferation of hepatocytes proportionally more intense the higher the elevation of
Avalia??o da express?o da caspase-3 no adenocarcinoma colorretal através de um sistema computadorizado de análise de imagem
Gomes, Monres José;Ribas-Filho, Jurandir Marcondes;Malafaia, Osvaldo;Czeczko, Nicolau Gregori;Ribas, Carmen Australia Paredes Marcondes;Nassif, Paulo Afonso Nunes;Bittencourt, Leandra Pauletto Muniz;
ABCD. Arquivos Brasileiros de Cirurgia Digestiva (S?o Paulo) , 2008, DOI: 10.1590/S0102-67202008000100007
Abstract: background: colorectal cancer is one of the most common causes of death for malignant neoplasm in the occidental world, corresponding colorectal adenocarcinoma to represent more than 90% of malignant tumors in large bowel. caspase-3 cysteine protease, one of the most important enzymes related in the apoptosis process, may be used as a tumor marker. aim: to identify and quantify caspase-3 expression in colorectal adenocarcinoma samples and to correlate the marker expression using an image computerized analysis system. method: caspase-3 expressions were studied by immunohistochemistry in 55 colorectal adenocarcinoma sampled-tissues, prepared in paraffin blocks. samba 4000 system was used for the immunostained tissues analysis. microscopic evaluation was performed by the imunno 4.00 software, which analyzed 8 to 10 fields in order to determine two parameters: labeling index (li), indicating the percentage of cells specifically stained by immunohistochemistry, and mean optical density (mod), representing enhance intensity, measured by positive pixels. for the statistical analysis, t-student test and pearson coefficient verified the possible correlation concerning li and mod. results: all samples presented positivity for caspase-3 expression (li 85,24% ± 8,71% positive cells). caspase-3 expression revealed statistically significance correlation between li and mod (p = 0,013). conclusions: the intensity of caspase-3 expression and the number of positive cells expressing such a marker showed a positive and significant correlation.
CD34 e caspase-3: compara??o, correla??o de express?o e quantifica??o imunoistoquímica no adenocarcinoma gástrico
Silva, Paulo Henrique Freitas Farias;Cuenca, Ronaldo Mafia;Ribas-Filho, Jurandir Marcondes;Ribas, Carmen Australia Paredes Marcondes;Malafaia, Osvaldo;Dobrowolski, Samuel;Bora, Fabio Roberto;
ABCD. Arquivos Brasileiros de Cirurgia Digestiva (S?o Paulo) , 2008, DOI: 10.1590/S0102-67202008000400006
Abstract: background: gastric cancer continues to defy the modern medicine in the understanding of its biological behavior and surgical cure. aim: to determine, compare and correlate the expression of caspase-3 and cd34 in gastric adenocarcinoma with clinical and pathological factors and proximal/distal gastric tumors. methods: imunohistochemistry quantitative analysis of the antibodies anti-caspase-3 polyclonal of rabbit and cd43 mouse's monoclonal, to mark adenocarcinoma cells in 26 cd34's cases and 22 caspase-3 paraffined blocks were used. the parameters were: labeling index in percentage and optic density. results: caspase-3 and cd34 expression were elevated; the average and the deviation standard for labeling index were respectively 87,72% ±7,89 and 84,86% ±9,17; for optic desity 60,75±7,46 and 49,84±8.48. when markers were compared with optic density it was found significant differences between caspase-3 (60,94) and cd34 (50,27). caspase-3 average (60,94) was higher than cd34 (50,27), p<0.001. the correlation among markers was positive for cd34's optic density versus labeling index of cd34 (p=0.006, r=0,520) and for optic density of caspase-3 versus caspase-3 labeling index (p=0.015, r=0.512). the markers had no correlation in the comparison of labeling index and optic densities with the gastric curvature, tumor differentiation, borrmann classification and lymphatic invasion. gender and age also did not correlate with the markers. the correlation with the tumor location had differences favoring the ones on distal part. conclusion: caspase-3 and cd34 expressions are elevated in gastric adenocarcinoma. caspase-3 optic density is higher than cd34's optic density. there is no expression and quantification correlation between markers and the clinical and pathological factors, but there are evidences that they have biological differences when in different gastric anatomical parts.
Action of tacrolimus on Wistar rat kidneys implanted with Walker 256 carcinosarcoma
Inácio, Cristiano Machado;Dietz, Ulrich Andréas;Malafaia, Osvaldo;Ribas Filho, Jurandir Marcondes;Nassif, Paulo Afonso Nunes;Czeczko, Nicolau Gregori;Marcondes, Carmen Australia Paredes;
Acta Cirurgica Brasileira , 2010, DOI: 10.1590/S0102-86502010000100020
Abstract: purpose: to evaluate the development of walker 256 tumor in male wistar rats treated with tacrolimus using an experimental kidney tumor model. methods: 40 male wistar rats were divided into four groups: tumor group (tu) (n=10), tacrolimus-tumor group (tt) (n=10), tacrolimus group (tc) (n=10) and control group (c) (n=10). treatment with tacrolimus was performed in groups tt and tc. under anesthesia, the right kidney of each animal of tu and tt was accessed through a supraumbilical incision and inoculated with a 0.1ml solution containing 2x106 tumor cells (walker 256 carcinosarcoma tumor cells). group tc was treated with a saline solution. all the animals of groups tc and tt were treated with tacrolimus (5mg/kg/day) by gavage for 15 days. tu group animals received saline by gavage for 15 days. on the 15th postoperative day, all animals were submitted to euthanasia and blood sampling for analysis of serum creatinine (cr) and blood urea nitrogen (bun). abdominal gross examination was performed, the right kidney removed and prepared for histological analysis by hematoxylin-eosin staining. the resulting data were submitted to statistical analysis by anova. results: statistical significance was found when comparing creatinine level between groups tu, tt and tc -tt group culminated with a marked increased in creatinine levels (cr=1.013 ± 0.3028 mg/ml), tu group (cr=0.5670 ± 0.03536 mg/dl) p=0.00256, tc group (cr =0.711 ± 0.1653 mg/ml) p= 0.02832. statistical significance was found when comparing bun levels in tt group (71.32 ± 17.14 mg/ml), compared with tu group (45.83 ± 5.046 mg/dl), p=0.000318. there were no statistically significant differences between groups tt and tc (61.23 ± 9.503 mg/ml) p=0.7242. histological analysis showed a poor evolution in tt group with multiple foci of hemorrhage and cortical invasion by the walker tumor. conclusion: the tacrolimus-treated group developed a more aggressive tumor and a drug-related nephrotoxic effect.
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